Tài liệu Báo cáo khoa học: Reductive nitrosylation of ferric human serum heme-albumin docx

... et al. Reductive nitrosylation of HSA-heme-Fe(III) FEBS Journal 277 (2010) 2474–2485 ª 2010 The Authors Journal compilation ª 2010 FEBS 2485 Reductive nitrosylation of ferric human serum heme-albumin Paolo ... endows human serum albumin (HSA) with heme-protein-like reactiv- ity and spectroscopic properties. Here, the kinetics and thermodynamics of reductive nitros...

Ngày tải lên: 16/02/2014, 14:20

... problem of unscrambling English affixes. The role of affixes is crucial in the study of word-breaking practice. In the automatic determination of the parts of speech (a central feature of automatic ... determination of parts -of- speech assignments E. The determination of the number of phonetic syl- lables corresponding to a written English word In the first case, we hav...

Ngày tải lên: 19/02/2014, 19:20

... classification of the weak suffixes by degree of weakness as a means of approaching the suffix-in- context problem. Table 2, which reviews the prefixes and suffixes de- fined by Resnikoff and Dolby, ... English," an algorithm for the structural definition of affixes was developed and applied to data consisting of all the words of the form CVCVC in the Shorter Oxford Dict...

Ngày tải lên: 19/02/2014, 19:20

... molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of EPSPS EPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate ... however, was devoid of substrate or inhibitor, and consequently did not reveal the nature of the active site or the mode of action of glyphosate. A dec- ade later, the cry...

Ngày tải lên: 14/02/2014, 14:20

... domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform of tau in the human central ... of tau with Fyn-SH3. We also show that tau interacts with the SH2 domain of Fyn, and that this associa- tion, unlike that of Fyn-SH3, is influenced by Fyn-mediated tyrosine ph...

Ngày tải lên: 14/02/2014, 14:20

... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL )1 and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution. Initial screens ... buffer at pH 6.0 (25 °C). Structural formulas of the substrates are presented. Redox potentials (E°)ofT1 coppers of the laccases and redox potentials of the substrates at pH 4.5 and...

Ngày tải lên: 14/02/2014, 18:20

... myoglobin, where the S -nitrosylation of a Cys residue causes a structural domain shift [33]. Whether the S -nitrosylation of Cys234 results in a similar structural rearrangement of CLIC4, and provides ... reservoir consisted of 500 lL of 0.7 m sodium citrate, 10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Drops consisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL )1 , 0....

Ngày tải lên: 14/02/2014, 19:20

... of that pathogen against that host background. It was found that 99.99% of all possible 11-mers, 70% of all 15-mers and 5% of all 18-mers are present in the human genome [16]. A select few human- blind’ ... specific amplification of DENV cDNA in the presence of excess human DNA. The mass ratio of DENV to human DNA was 1 : 1000 and the molar ratio was 1 : 0.005 (a molar ratio...

Ngày tải lên: 14/02/2014, 19:20

... tempera- ture or the speed of synthesis and decay of the RNA molecule [15,16], are major determinants of the folding kinetics. Further factors that affect the speed and reac- tion route of RNA folding are ... to millimolar range, because the off-rates are high (k off ‡ 0.2 s )1 ) [25]. A further way of describing macromolecular complexes is by the molecular interface of the intera...

Ngày tải lên: 14/02/2014, 19:20

... (Fig. 3A). Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor. Figure 3D shows the superimposition of the active site structures of...

Ngày tải lên: 14/02/2014, 22:20