Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc

... Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer fluorescence lifetime imaging microscopy in living ... dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) was visualized in living CHO-K1 cells by fluorescence resonance energy trans...

Ngày tải lên: 16/02/2014, 09:20

... rationalized by invoking better binding of protein to ssDNA compared to dsDNA under these conditions, thereby leading to suppression of ssDNA renaturation, as two interacting protein–ssDNA com- plexes ... protein coats single stranded DNA (ssDNA) as a helical filament (three nucleotides per protein monomer) resulting in stretching of DNA by 1.5 times its original length. This conformatio...

Ngày tải lên: 19/02/2014, 07:20

... nucleolar protein involved in ribosomal RNA processing in Xenopus laevis oocytes and mammalian cells. Downregulation of Gua using small interfering RNA (siRNA) in HeLa cells resulted in 80% inhibition ... regulated by protein–protein interactions. To determine the RPL4 domains involved in Gua interaction, we generated FLAG-tagged human RPL4 deletion mutants (Fig. 4A,D), exp...

Ngày tải lên: 20/02/2014, 01:20

... substrate binding pocket in such a way as to interfere with substrate binding when masked by interaction with polyanions. Interactions between modifiers and elastase-2 P. Schenker and A. Baici 2488 ... glycosaminoglycans is best represented in this situation by measuring the surface available to enzyme binding, as reported in a study of cysteine pep- tidases binding to insolu...

Ngày tải lên: 16/02/2014, 14:20

... proteins [13] and binding interactions [14]. FRAP is a conventional technique used to study the kinetic properties of proteins in a cell by measuring the fluorescence recovery rate in a bleached ... microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudor coding sequences were joined to the C-terminal region of the GFP or RFP coding sequen...

Ngày tải lên: 18/02/2014, 16:20

... out by heterologous expression in yeast. Results and Discussion P450 genes induced by elicitors in the cell culture of G. max Accumulation isoflavones phytoalexin glyceollins, in the seedling infected ... saponins are glycosides of cyclic C30 terpe- nes and include a number of active constituents of medicinal plants, as exemplified by glycyrrhizin in Glycyrrhiza glabra, ginsen...

Ngày tải lên: 19/02/2014, 07:20

... T-cell kinase (ITK) could in uence the infectivity of HIV and also have anti -in ammatory activity. Since 2006, several patients carry- ing a fusion protein, originating from a translocation joining ... mutation in the BTK PH domain; arginine 28 is in dark blue, encircled in red. Bottom left: SH2 domain. Right: kinase domain. The mutated residues are indicated in yellow, a-heli...

Ngày tải lên: 14/02/2014, 18:20

... threonine and one serine in embryonic kid- ney, three serines and two threonines in leukemia; whereas two tyrosine residues were found in both leu- kemia and lung cancer and one serine in both ... [44] and gangliosides [14]. ENOA and most of these proteins have C-terminal lysines predominantly responsible for plasminogen acti- vation [45]. Interaction of the plasminogen...

Ngày tải lên: 14/02/2014, 19:20

... GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrase domain; GSGB, glucosyltransferase-I containing a full-length glucan-binding domain; GTF, glucosyltransferase. FEBS ... water-insoluble a-(1 fi 3)-glucan, respectively. They share highly homologous amino acid sequences and comprise the N-terminal glucansucrase domain (GSd) and the C-term...

Ngày tải lên: 14/02/2014, 22:20

... The intensity of the band of the protease domain was quantified using bovine serum albu- min (BSA) as a standard. The enterokinase-treated pro-TMPRSS13 obtained from COS-7 cells was separated by ... lipoprotein (LDL) receptor class A domain (L) between the Kunitz domains (Fig. 2A). Inhibition by aprotinin was compared with that by HAI-1, because aprotinin has been shown to efficiently...

Ngày tải lên: 15/02/2014, 01:20