Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... For the superposi- tion of the substrate- free PhyK with substrate- free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate- free PhyK and the substrate- loaded AppA the averaged distance ... ions bound at the active site, a model of phytate bound to PhyK was calculated, so that the sulfate positions mark the sites of phytate phosphate gr...

Ngày tải lên: 16/02/2014, 09:20

... weak homodimer, as observed for MaL, that may determine the properties of these asco-laccases at high protein concentrations. Database Structural data are available in the Protein Data Bank database ... (asco-lac- cases) have been rather unexplored, and to date only the crystal structure of Melanocarpus albomyces laccase (MaL) has been published. We have now solved the cr...

Ngày tải lên: 14/02/2014, 18:20

... B z is the normalized B factor, B i is the B factor of atom i, l j is the mean B factor (excluding water molecules and peptide atoms) and r j is the standard deviation of B factors. The normalized ... importin -a structure that incorporates the N-terminal autoinhibitory domain (PDB:1IAL, rmsd of 0.20 A ˚ across 425 C a atoms in residues 72–496). The major binding...

Ngày tải lên: 14/02/2014, 19:20

... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The angles around the metal cofactor sug- gested ... octahedral coordination around the metal cofactor. The absence of an anomalous Fourier map demon- strated that the active site in apo-ApeSOD did not c...

Ngày tải lên: 14/02/2014, 22:20

... extracellular matrix, it seems more likely that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ... active site and are named according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- a...

Ngày tải lên: 19/02/2014, 00:20

... crystal structure. The binding of acetate in the active site is a further indication that the enzyme may be involved in deacetylation because acetate is one of the reaction products. Asp14, a residue of the ... Asp residues (Asp15 and Asp12, respectively), adopt a main chain conformation that deviates less from the stand- ard values. It appears that t...

Ngày tải lên: 19/02/2014, 00:20

... 1THM share an ion pair arrangement, Asp56–Arg95 and Asp59–Arg95 (numbers relate to 1SH7), connecting the surface loop that hosts their common calcium- binding site to a site proximate to the substrate- bind- ing ... active site of 1SH7 consists of the catalytic triad Asp37, His70 and Ser220, and substrate recognition and binding sites that are well conserv...

Ngày tải lên: 19/02/2014, 16:20

... 5¢-CAGCCAAGCG CCTAGGGAGCGAGA AC-3¢, containing a BamHI site (underlined) and starting 31 nucleotides downstream of the stop codon. The total volume of the amplification mixture was 50 lL containing 1 ... Biochemical studies with the Trypanosoma cruzi axenic amastigote intracellular form also suggest that carbohydrate catabolism is its major source of energy [4]. The glyco...

Ngày tải lên: 20/02/2014, 02:21

... decarboxylase, suggesting that the interactions with the cofactor thiamin diphosphate and the catalytic mechanisms are very similar. The substrate binding site in indolepyruvate decarboxylase contains ... protein atoms. The final R-values and other refinement statistics are given in Table 1. The X-ray data and the atomic coordinates have been deposited at the Protein Dat...

Ngày tải lên: 20/02/2014, 11:20

... to the catalytic H113, is in a simi- lar orientation to that in the active site of nonspecific RNases. These novel architectural features lead to new electrostatic interactions at the active site ... were analyzed in a semi-automated iterative manner by cyana 2.1 [38]. The NOE coordinates and intensities used as input for automated analysis were generated auto- ma...

Ngày tải lên: 18/02/2014, 08:20