Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... surprising that TH8 is in the core because it is buried in the structure. Within the whole toxin, TH5 is partly covered by the C-terminal part of the receptor (R) domain [2,3]. Within the isolated T domain, ... to the functional state, which initi- ates the translocation of the catalytic domain. Here, the data allowed identification of the core of the...

Ngày tải lên: 16/02/2014, 09:20

... delta in mammalian replication, with the fast sedimenting Triton- extracted fraction. In a first experiment we employed this protocol thereby demonstrating that important components of the ‘classical’ prereplication ... nuclei by Triton X-100 contain- ing buffer, and in fraction 3, the chromatin fraction, all proteins remain that resist Triton extraction. We supposed that the latt...

Ngày tải lên: 21/02/2014, 00:20

... has received great attention over the past years using knockout and transfection technologies. In marked contrast, studies addressing the effect that iron loading of hepatic cells has on the expression ... class I molecule localizes in the cytoplasm of human epithelial cells of the gastrointestinal tract and liver, two central organs in the regulation of iron metabolism [2...

Ngày tải lên: 19/02/2014, 16:20

... causes the basicity of the histidine to increase, so that it is able to accept/abstract the Oc proton from the catalytic serine. Transferring a proton from the catalytic serine to the catalytic histidine ... recipient for the serine proton is the catalytic histidine. Most models use the histidine to shuttle protons: first, between the serine and the leaving group; and the...

Ngày tải lên: 20/02/2014, 23:20

... about 50% in macrophages exposed to hypoxia, thus indicating a major change in the CytOX content in these cells. These results suggest that the CytOX activity in the two cell types is differentially ... also seen that the level of ATPase complex as determined by immunoblotting with antibody to the F 1 ATPase did not change under these conditions (Fig. 3B). Quantification...

Ngày tải lên: 20/02/2014, 23:20

... were collected in the supernatant and their concentration was determined using the Bio-Rad Protein Assay (Bio-Rad). All experiments were run in triplicate. In this way, three independent samples ... of a-synuclein are present, either as a consequence of transfection or of dopamine treatment (Fig. 1), sug- gesting that a-synuclein could at least contribute to the deactivation...

Ngày tải lên: 15/02/2014, 01:20

... allowing identification of the core domain. Then, it is necessary to confirm that the protein has the target O-glycosyla- tion by an alternative method, although it remains difficult to confirm the O-glycan ... method to identify carrier proteins with the specific lectin epitope. Using this strategy, we have identi- fied many glycoproteins containing glycan structures that are altered...

Ngày tải lên: 16/02/2014, 08:20

... generated inducible transgenic mice that express a dominant-neg- ative SNARE domain selectively in astrocytes to block the exocytotic events in astrocytes. Using the trans- genic mice, they demonstrated ... gliotransmitters through which astrocytes can actively regulate synaptic transmission. ATP differs from glutamate in that it inhibits rather than potentiates synaptic transmi...

Ngày tải lên: 16/02/2014, 09:20

... data strongly argue that the therapeutic effect of the modified IVIg was not due to better neutralization of the injected LPS: first, the binding of the preparation to LPS in its two forms was ... salt concentration in the buffer. In contrast, this interac- tion was mostly independent of the salt concentration in the case when the low-pH buffer-exposed Z2 was left to...

Ngày tải lên: 16/02/2014, 15:20

... whereas the bound GCA mostly in uenced the C-terminal region of the protein, in agreement with the site selectivity observed for the two ligands. Interestingly, the heterotypic complex, in which the ... as in the adaptation to various pathological conditions, contributing to the maintenance of bile acid homeostasis and functional distribution within the cell....

Ngày tải lên: 18/02/2014, 06:20