Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... (sense) and 5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT ACTT-3¢ (antisense). Caspase 3 activity Cells ... Relative fluorescence intensity was determined using imagemaster 2d elite software 4.01 (Amersham Bioscience, Uppsala, Sweden). Statistical analysis Data in bar graphs are expressed as the me...

Ngày tải lên: 18/02/2014, 18:20

... Differential glycan profiling between cancer and normal cells enables identification of aberrant glycosylation in cancer [indicated as a red triangle in (B) and (C)] as an alteration in lectin signal pattern. ... MS. Abbreviations AAL, Aleuria aurantia lectin; AFP, a- fetoprotein; GGDB, GlycoGene Database; GlycoProtDB, GlycoProtein Database; GMDB, Glycan Mass Spectral Database; H...

Ngày tải lên: 16/02/2014, 08:20

... Narayanan and Jurafsky, 2002; Hale, 2001; Crocker and Brants, 2000). Jurafsky (1996) proposed a probabilistic model of HSPM using a parallel beam-search parsing technique based on the stochastic ... probability at each word break and probability re-ranking, if any. Note that the run- ning probability at the beginning of a sentence will be 1, and will keep decreasing at each word b...

Ngày tải lên: 20/02/2014, 11:21

... (4). (4) AG('-,r AAXr ~ AF(- ,a AAX (a AAF(r AAX( ,r AAF (a AAX ,a) ))))) Example (3b) parallels (lb) in being closer to CTL than its (a) counterpart. Nevertheless, (3b) is ontologically richer ... on the particu- lar task within a generic application domain, as well as on the class of users. As a final remark, we wish to point out that there may be advantages in id...

Ngày tải lên: 20/02/2014, 19:20

... PCR using the MluI ⁄ XhoI tailed primers DARSfw (5¢-ACTACGCGTAGTCCAAGAGAGGAGAAACC -3¢) and DARSrv (5¢-ACTCTCGAGCCCGGAGCGCTGGCG GCCGC-3¢), and NFKBIAfw (5¢-ACTGAGCTCCCGA CGACCCCAATTCAAATCG-3¢) and ... reverse, 5¢-TGTCACATGGCAGGGATGGC-3¢; and CASP4 for- ward, 5¢-GTCTGGCAACCCCTGTTGAAT-3¢; CASP4 reverse, 5¢-GCCTGCTGGCTCTGAAGAGTATC-3¢. Ampli- fication of a coding region part of the intron...

Ngày tải lên: 18/02/2014, 18:20

... Kaneko 1 , Yuanying Ding 1 , Kazuhiro Ogawa 2, *, Miki Yoshizawa 1 , Masaki Kawamura 1 , Kazuhisa Takeda 1 , Tadashi Yoshida 3 and Shigeki Shibahara 1 1 Department of Molecular Biology and Applied ... (Amersham Biosciences, Piscataway, NJ, USA). Expression of a- tubulin was examined as an internal control using a- tubulin mono- clonal antibody (NeoMarkers, Fremont, CA, USA). Assay fo...

Ngày tải lên: 19/02/2014, 06:20

... can increase the amount of available phosphate by interacting together. Additionally, fusing PhyH-DI to a single-domain phytase appears to be an efficient way to improve the activity of the latter. Introduction Phytate ... InsP 6 gradually into InsP 5 , InsP 4 , and the final product – Ins(2,4,6)P 3 and Ins(1,3,5)P 3 – via two alternative pathways [14]. Bacterial BPPs containing two tande...

Ngày tải lên: 14/02/2014, 15:20

... impor- tant for cellular ion relationships in natural conditions. Increasing in ux, decreasing efflux or both can maxi- mize K + uptake to maintain K + homeostasis in plants [32,33]. Using the noninvasive ... critical for the bio- genesis of microRNAs and Arabidopsis development [24,25]. Arabidopsis TEBICHI, containing an N-termi- nal DELH box RNA helicase domain and a C-termina...

Ngày tải lên: 14/02/2014, 18:20

... A, Milella M et al. (2009) An integrated humoral and cellular response is elicited in pancreatic cancer by alpha-enolase, a novel pancreatic ductal adenocarcinoma-associated antigen. Int J Cancer ... Sahara H, Miyazaki A, Nabeta Y, Hiroh- ashi Y, Kanaseki T, Yamaguchi A, Yamada N, Hiray- ama K, Suzuki M et al. (2002) Natural antigenic peptides from squamous cell carcinoma recognize...

Ngày tải lên: 14/02/2014, 19:20

... T, Sawodny O, Ederer M & Heyer AG (2010) Mathematical model- ling of the central carbohydrate metabolism in Arabid- opsis thaliana reveals a substantial regulatory in uence of vacuolar invertase ... simulation A mathematical model was developed, representing central carbohydrate metabolism in leaves of A. thaliana. The model was based on the following system of ordinary...

Ngày tải lên: 14/02/2014, 22:20