Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... of Met8P has shown that this protein has an aspartate residue (Asp141), which is important for both chelatase and dehydrogenase function [17]; interestingly, this aspartate, Asp129, is also conserved 98 kDa M ... Authors Journal compilation ª 2010 FEBS 4955 NirF is a periplasmic protein that binds d 1 heme as part of its essential role in d 1 heme bi...

Ngày tải lên: 15/02/2014, 01:20

... NS & Woude GF (2002) Apoptosis and melanogenesis in human melanoma cells induced by anthrax lethal factor inactivation of mitogen-activated protein kinase kinase. Proc Natl Acad Sci USA 99, 3052–3057. 21 ... effect of cleaving these mitogen-activated protein kinase kinases is to interfere with extracellu- lar signal-related kinase (ERK), p38 and c-Jun N-terminal kinase signali...

Ngày tải lên: 16/02/2014, 09:20

... The Authors Journal compilation ª 2010 FEBS bacitracin contains at least nine different peptides, of which bacitracin A is the most abundant, and it is mainly used as an antibiotic against infections ... well as the isolated catalytic a domain of PDI. Both the PDI a domain and DsbA have a catalytic site, with an associated substrate-binding site, but lack an inde- pendent...

Ngày tải lên: 16/02/2014, 14:20

... PA-TM-RING family have a protease- associated (PA) domain and a RING finger domain separated by a transmembrane (TM) domain. PA domains are 120–210 amino acid sequences located in the noncatalytic ... determinants on tobacco chitinase and barley lectin [14], establishing that in plants the PA domain can serve as a ligand-binding domain. The best-characterized mammalian PA-TM-RI...

Ngày tải lên: 18/02/2014, 08:20

... proteins), that differ substantially in their biochemical properties and intracellular localization. DAP-kinases (DAPk), Ca 2+ ⁄ calmodulin-regulated and Ser ⁄Thr kinases, activate signaling pathways that lead ... proteins plays major roles in a multitude of basic pathways during cell life and death, in both health and disease. The ubiquitin–proteasome pathway involves conjugati...

Ngày tải lên: 19/02/2014, 02:20

... studied human cathepsins [1]. This enzyme is abundantly expressed in a variety of tissues where it takes part in protein degradation and processing. It is involved in a number of physiological and pathological ... cathep- sin B structures are almost 10 A ˚ apart. It is concluded is that the occluding loop is rather flexible and will adapt to structural features...

Ngày tải lên: 18/02/2014, 04:20

... specificity of protein degradation is also a plausible mechanism to explain our data. In general, not all proteins are degraded to the same extent and at the same rate. The autophagy route to degradation, which ... i.e. the ethanol flux under anaerobic conditions at glucose excess, was mea- sured in an off-line assay. Because the fermentative capacity was measured in an off-line a...

Ngày tải lên: 18/02/2014, 06:20

... aeruginosa NirE The recombinant P. aeruginosa NirE protein was pro- duced either as a fusion protein carrying a C-terminal His-tag (NirE-His) or as a fusion protein carrying both N-terminal thioredoxin ... in Affymetrix microarray analyses of anaerobically grown Fig. 5. Growth curves of wild-type Pseudomonas aeruginosa PAO1 and of P. aeruginosa strain PAO1 ID35553. P....

Ngày tải lên: 18/02/2014, 06:20

... ligand binding analysis (data not shown) revealed a 50% decrease in B max , whereas the affinity remained unchanged. This surpris- ing finding led us to hypothesize that the large multi- valent Man-6-P-based ... Recombinant human IGF-II was a gift of Lilly Research Laboratories (Indianapolis, IN, USA). The pseudoglycoprotein PMP-BSA was pre- pared as described previously [34]. Radi...

Ngày tải lên: 18/02/2014, 08:20

... and functional properties of RNase and participates in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek Jura 3 , Barbara ... was hybridized with a radioactively labelled MCPIP cDNA probe and visualized by autoradiography. A b-actin probe served as a control for equal loading. A single 2.0 kb band...

Ngày tải lên: 18/02/2014, 14:20