Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... sub- strate and quantified by using the DE- 81 filter paper S103 Y 112 R207 /19 9 K173 /16 5 G145/D137 D142 /13 4 E1 41/ 133 L75/67 D2 01/ 193 G77/69 G197 /18 9 G145/D137 A B Fig. 2. Superposition of PRTFDC1 with ... 10 6 ± 1. 0 · 10 6 (10 0%) Guanine PRTFDC1 36 .1 ± 14 .3 2.9 ± 0.7 1. 36 ± 0.34 3.9 · 10 4 ± 9.3 · 10 3 (0.09%) HPRT 9.9 ± 0.2 899 ± 11 7 406 ± 53 4.5 · 10...

Ngày tải lên: 15/02/2014, 01:20

... 77 61 55 β3 β4 α2 1 α3 β6 α4 β7 13 β8 α5 12 η2 η3 α6 1 β9 10 11 β2 1 β5 11 1 Active site 12 0 14 7 15 5 227 17 3 213 247 234 205 19 9 19 5 2 1 5 5 12 8 13 9 10 11 A B C Fig. ... <I hj > is the jth observation of reflection h and <I h > is its mean intensity. 2 1 3 4 3 1 6 18 9 18 5 17 9 17 8 207 16 7 16 5 10 3 98...

Ngày tải lên: 18/02/2014, 14:20

... K i C AB B A A B E204 E204 T197 T197 A C F133 F133 T1 31 T1 31 A C D E A K102 D104 K102 D104 D104 K102 C E D Fig. 3. (A) Interactions between subunits A, B and C. (B) Hydrophobic interaction between the a4 helices ... 0.05 and 0.85 ± 0.05, respectively (Fig. 9). At 1 mm ATP, the K 0.5,app (UMP) and V max,app values for F133N were 11 00 ± 15 0 lm and 10 7 ± 15 lmolÆ min )1...

Ngày tải lên: 18/02/2014, 16:20

... revised 11 July 2006, accepted 27 July 2006) doi :10 .11 11/ j .17 42-4658.2006.05430.x The vertex of the adenoviral capsid is formed by the penton, a complex of two proteins, the pentameric penton base and ... clathrin-coated pits and endosomes [14 ,16 ]. It is thought that upon integrin binding and before endocytosis, the fiber protein is shed from the virus [13 ,17...

Ngày tải lên: 19/02/2014, 06:20

... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 in Fig. 5) defines the allowable movements of the FMN subdomain and thus controls the FMN–NOSoxy ... 4 419 5, USA Fax: 1 216 636 010 4 Tel: +1 216 445 6950 E-mail: stuehrd@ccf.org (Received 13 March 2009, revised 18 May 2009, accepted 28 May 2009) doi :10 .11 11/ j...

Ngày tải lên: 18/02/2014, 11:20

... 2.76 a 0. 51 ± 0.04 [15 ,18 ] MR b-NADH 6.8 ± 0 .1 a 7.4 ± 1. 5 a 0 .12 ± 0.09 [15 ,18 ] MR cylohexen -1- one 3.5 ± 0.2 )0.5 ± 1. 8 4 ± 2 [15 ] GO 2-deoxyglucose $10 b $0 b $10 g [73] CO choline 10 .6 ± 0.6 b 1. 0 ... Manchester M1 7ND, UK Fax: +44 16 1 306 8 918 Tel: +44 16 1 306 515 2 E-mail: nigel.scrutton@manchester.ac.uk (Received 23 December 2008, revised 28 April 2009, acce...

Ngày tải lên: 18/02/2014, 11:20

... hydride transfer [11 4– 11 7 ,11 9]. S223 and Y235 at the AnFNR 2¢P-AMP site are critical in determining the specificity and efficient coenzyme orientation [99 ,11 5 ,12 1]. The 15 5 16 0 and 2 61 268 loops, which ... affinity and selectivity [11 7 ,11 8 ,12 2 12 6]. Structural studies have allowed us to postulate a stepwise mechanism in which the nucleotide must bind to a...

Ngày tải lên: 18/02/2014, 11:20

... a Fig. 2. The CUB domain. The CUB domains of PDGF-C and PDGF-D aligned with the prototype CUB domains of human neuropilin (acces- sion no CAI402 51) and human bone morphogenic protein- 1 (BMP -1, accession ... urchin EGF-like protein and bone morphogenic protein 1 [16 ]. These proteins are often referred to as the prototype CUB domains. Like the prototype CUB...

Ngày tải lên: 19/02/2014, 07:20

... IFP–F13 IFP–wt IFP–F13 Secondary structure percentage a-Helix 57 ± 3 66 ± 3 60 ± 4 64 ± 3 Disorder 12 ± 2 11 ± 3 12 ± 3 11 ± 3 b-Sheet 24 ± 1 20 ± 2 22 ± 1 22 ± 1 b-Turn 7 ± 1 3 ± 2 6 ± 1 3 ± 1 Helix ... in the region 10 18 and 20– 26. (Lower) Helical segments can b e observed i n 1 11 a nd 14 –28 by contiguous (i,i +3) interactions. The absence of (i,i +3) cross-pe...

Ngày tải lên: 19/02/2014, 16:20

... 29–33) and C (position 35) as compared to GenBank AF 111 246 [ 61] . In the EST clone, the asparagine at position 42 of GenBank AF 111 246was replaced with a lysine. The cDNA was digested with NdeI and ... activity (lmolÆmin )1 Æmg )1 ) CuOOH GPx activity (lmolÆmin )1 Æmg )1 ) Activity of purified enzyme Human MGST1 [ 71, 72] 1. 9 0.04 Rat MGST1 [43] 2 0.08 Xenopus lae...

Ngày tải lên: 19/02/2014, 17:20