Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... authors. Regulation of cytosolic 5¢-nucleotidase R. Pesi et al. 4872 FEBS Journal 277 (2010) 4863–4872 ª 2010 The Authors Journal compilation ª 2010 FEBS Active and regulatory sites of cytosolic 5¢-nucleotidase Rossana ... (MI:0071) Abbreviations cN-II, cytosolic 5¢-nucleotidase; cN-III, cytosolic 5¢-nucleotidase III; cN-IA, cytosolic 5¢-nucleotidase IA; cN-IB,...

Ngày tải lên: 15/02/2014, 01:20

... DNA and RNA were performed as described previously [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the ... Characterization of MAPEG members FEBS Journal 272 (2005) 1688–1703 ª 2005 FEBS 1699 BamHI and EcoRI and then the reformatted BamHI-EcoRI fragment was subcloned into BamHI and Ec...

Ngày tải lên: 19/02/2014, 17:20

... set and Annotation Corpus: Two college-educated native speakers of Arabic annotated 2855 sentences from Part 1 V 3.0 of the PATB. The sentences make up the first 400 documents of that part of PATB amounting ... expression of opinions, evaluations, feelings, and speculations (Banfield, 1982; Wiebe, 1994) and thus incorporates sentiment. The process of subjectivity classification...

Ngày tải lên: 20/02/2014, 05:20

... concentrations and metabolic fluxes, with the frequency of oscillation and with the majority of other experimentally known kinetic and dynamical variables ODE sim, fit, osc Own software BM 61, ... a key role and allosteric inhibition of PFL and altered pfl gene expression are important in the regulation of the shift ODE sim, fit NG NG Time series of proteins measured by ELISA...

Ngày tải lên: 14/02/2014, 14:20

... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and conformation of proteins. I. Effect of urea ... Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, Universi...

Ngày tải lên: 14/02/2014, 22:20

... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with coordination distances of approximately 2.4 A ˚ . The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino ... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using th...

Ngày tải lên: 15/02/2014, 01:20

... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were ... Homology model of HYD Js . (A) Docking of D-p-HPH to the HYD Js active site. (B) The SGLs of HYD Bp are shown as magenta lines and those of HYD Js are shown in green. The...

Ngày tải lên: 18/02/2014, 08:20

... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational change rates versus rates of FMNH 2 formation ... Model and simulations of cytochrome c reduction by NOS enzymes. (A) Scheme of cytochrome c reduction. The model uses four kinetic rates: dissociation (k 1 ) and association (...

Ngày tải lên: 18/02/2014, 11:20

... & Buckmann AF (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo- specificity of 7-alpha-hydroxysteroid and 12-alpha-hy- droxysteroid dehydrogenase. ... isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. Biochem...

Ngày tải lên: 18/02/2014, 11:20

... by docking of Fld onto FNR (Fld in green, light orange and pink correspond to docking solutions ranked 1, 3 and 5 respectively). (B) Detail of the proposed FNR active site centre in a model of ... and pyridine nucleotides is highly dependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FN...

Ngày tải lên: 18/02/2014, 11:20