Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... from A. pernix K1 FEBS Journal 278 (2011) 59 8–6 09 ª 2010 The Authors Journal compilation ª 2010 FEBS 609 Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights ... 2010) doi:10.1111/j.1742-4658.2010.07977.x Aeropyrum pernix K1, an aerobic hyperthermophilic archaeon, produces a cambialistic superoxide dis...

Ngày tải lên: 14/02/2014, 22:20

... in IPDC extends from the thiazolium ring of the cofactor to the surface of the protein. The entrance of the active site cleft is covered by the C-terminal helix and this part of the polypeptide ... crystallography. The fold of the subunit is similar to that of ScPDC [14] and ZmPDC [15]. However, the packing of the two dimers in the tetramer is dif...

Ngày tải lên: 20/02/2014, 11:20

... them difficult to handle. The crystals were approximately 0. 3–1 mm long and 0.1 5–0 .5 mm wide, and the thickness of the crystals was always under 0.1 mm. Data collection and structure refinement Before ... [21], and the more recently published novel two-domain laccases [2 2–2 4]. The other phylum of the fungi, the Ascomycota or sac fungi, is much less studied...

Ngày tải lên: 14/02/2014, 18:20

... mgÆmL )1 and 1 lLof reservoir. These conditions resulted in a 7.6 times molar excess of the CLIC4 NLS peptide over importin-a (7 0–5 29). There was no incubation of peptide and protein other than the ... were recalculated. The normalized B factors from apo importin-a were then subtracted from those in the importin-a:CLIC4 NLS structure, and the difference was again...

Ngày tải lên: 14/02/2014, 19:20

... is the only direct contact between the protein backbone and the substrate. The side chain of Thr292 is found to recognize the 2-phos- phate in both the binding model of PhyK and the crys- tal structure ... which three of six phosphate groups of phytate perfectly fitted into the electron density occupied by sulfate ions in the crystal structure, suggesting...

Ngày tải lên: 16/02/2014, 09:20

... reviews [1,2,10]. S1 The following segments border the S1-specificity pocket of DESC1: Asp189–Gln192 (the basement of the pocket), Ser214–Gly219 (the entrance frame), Lys22 4– Tyr228 (the back of the pocket) and the ... planes of the bonds between Trp215–Gly216 and Cys191–Gln192 sandwich the phenyl ring of benzamidine. The DESC1 S1 pocket resembles the thr...

Ngày tải lên: 19/02/2014, 00:20

... infectiousness of the latter. The objective of the present study is to shed light on the structure, function and the structure function relationships of one B. cereus protein, a pro- duct of the bc1534 ... accessibility and determines the shape of the active site entry. (e) The structure of the active site is essentially identical with the active sit...

Ngày tải lên: 19/02/2014, 00:20

... album Limber and thermi- tase from the thermophilic eubacterium Thermoactino- mycetes vulgaris. Results The crystal structure of the Vibrio proteinase The obtained Vibrio proteinase crystals formed ... 1. (A) Model of the crystal structure of the Vibrio proteinase. The residues of the catalytic triad, S220, H70 and D37 are shown in yellow, the calcium i...

Ngày tải lên: 19/02/2014, 16:20

... residues 6 5–7 4, 9 9–1 03 and 11 7–1 21 in monomer C and 9 9–1 02 in monomer B and several residues at the N-terminus and C-terminus, which are poorly resolved. The stereochemistry of the structure is ... Despite the large number of crystallographically determined 3D structures of GAPDHs from several organ- isms [1 6–2 9], there is none giving the detail...

Ngày tải lên: 20/02/2014, 02:21

... activity of mutants L160T, L160P, K13 3A, K13 3L, K13 3S and K13 3T dropped to less than 5%. When guanine was used as the substrate, the activity of L160T, K13 3A, K13 3T, K13 3V fell to 18%, 14%, 13.8% and ... and crystals. The N-terminus of each subunit of the tetra- mer forms crystal contacts with the active site of a subunit of the neighboring tetram...

Ngày tải lên: 23/03/2014, 07:20