Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... 2010 The Authors Journal compilation ª 2010 FEBS Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver ... constitutes the initiating event in the process of lipotoxicity. Stearate has to be activated in order to be toxic The first enzyme inv...

Ngày tải lên: 14/02/2014, 22:20

... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidative stress on the surrounding tissue, releasing large amounts ... cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform activities and gene express...

Ngày tải lên: 14/02/2014, 18:20

... in skin barrier disruption (Fig. 1A). PPARb mRNA expression was determined in keratinocytes isolated from the epidermal layer of these skins, and was found to be significantly increased in skin ... and increases CerK activity in SP1 cells To investigate the role of CerK and its regulation of Cer levels in the effect of PPARb activation on cell survival, the expressi...

Ngày tải lên: 18/02/2014, 18:20

... of alternative promoters in DNASE1 results in no generation of protein isoforms. The 5¢-UTR of eukaryotic mRNA in uences the initi- ation step of protein synthesis and thereby in part determines ... respectively. Con- ditions for these amplifications were 95 °C for 9 min, 37 cycles of 94 °C for 1 min, 65 °C for 1 min, and 72 °C for 2 min, followed by incubation...

Ngày tải lên: 19/02/2014, 06:20

... Histograms of the height differences between the top of SERCA and the surface of the membrane. Statistical section analy- ses of SERCA were performed with the data obtained in the pres- ence of ... ATP hydrolysis of SERCA linearly increased with ATP concentrations of  1 lm [26]. The lifetime of the elevated conformation (i.e. peak width) in the presence...

Ngày tải lên: 14/02/2014, 14:20

... by the proline in the cis confor- mation. Indirectly, we have confirmed that proline isomeriza- tion is at the root of the slow conformational change coupled to tetramerization by measuring the ... dependence of the kinetics [123]. The value for the activation energy of 28 ± 3 kcalÆmol )1 observed for the P79S mutant tetramer formation is consistent with the...

Ngày tải lên: 14/02/2014, 18:20

... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric protein decreased, whereas the bands for the higher ... Nota- bly, in the presence of the neurotoxin, there was a delay in the lag time for fibrillation. The lag time increased from 74.9 h in case of a -syn...

Ngày tải lên: 14/02/2014, 19:20

... Cyclo- philin D binding decreased both ATP synthesis and hydrolysis rates. In the present study, we reaffirm these findings by demonstrating that, in intact mouse liver mitochondria energized by ATP, the ... [1,2], is of a sufficient size (cut-off of $ 1.5 kDa) to allow the pas- sage of solutes and water, which may also result in rupture of the outer membrane. The i...

Ngày tải lên: 14/02/2014, 19:20

... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-cadherin and b-catenin ... membrane proteins to migrate apically. Transcytosis of integrins would therefore enable H. pylori with the intriguing possibility of targeting the integrin b 1 receptor at api-...

Ngày tải lên: 14/02/2014, 19:20

... course of dextran-independent glucan synthesis We examined the kinetics of insoluble glucan synthesis from sucrose by GS and GSGB in the absence of dex- tran (Fig. 3A,B). For this purpose, we incubated sucrose ... branches on the primer dextran bound to the C-terminal glucan-binding domain. We investigated the GTF- I-catalyzed glucan synthesis reaction in the abs...

Ngày tải lên: 14/02/2014, 22:20