Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in fish pdf

... downregulates aromatase expression [37]. Overall, it is now clear that, at least in fish Dmrt1- regulating aromatase expression and by implication the estrogen ⁄ androgen balance that would also feedback ... abundant dmrt1 expression during preparatory and prespawning and spermatogenesis periods was seen, in contrast to a gradual decrease thereafter during spawning ⁄ spe...

Ngày tải lên: 14/02/2014, 19:20

... reference genes: elongation factor 2 (EF-2), 5¢-GACATCACCAAGGGTGTGCA-3¢ (forward) and 5¢-TTCAGCACACTGGCATAGAGGC-3¢ (reverse); GAPDH,5¢-CCGAGCCACATCGCTCAGAC-3¢ (forward) and 5¢-GTTGAGGTCAATGAAGGGGTC-3¢ (reverse). ... in degradation of IL-1b mRNA Danuta Mizgalska 1 , Paulina We˛ grzyn 1 , Krzysztof Murzyn 2 , Aneta Kasza 1 , Aleksander Koj 1 , Jacek Jura 3 , Barbara Jarza˛b 4 and Jol...

Ngày tải lên: 18/02/2014, 14:20

... specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria Raka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta and Sampa ... of enzymes. Three papain-like cysteine proteases, ervatamin -A, ervatamin-B and ervatamin-C, have been isolated and purified from the latex of a medicinal plant Erva...

Ngày tải lên: 18/02/2014, 16:20

... termed Adracalin or AAAS) and nup62, can be added to the expanding list of mutated nucler lamina proteins causing these diseases. Muta- tions in the WD-repeat ALADIN NPC protein cause the Triple A ... that are deficient in lamins, and that this fragility could ultimately lead to nuclear damage and cell death [68]. The idea of enhanced nuclear fragility is particularly a...

Ngày tải lên: 19/02/2014, 02:20

... of the initial response to DSBs is the rapid activation of ATM (product of the ataxia telan- giectasia mutated gene), the protein kinase that is mutated in the human hereditary disease ataxia ... precipitation was a uniquely effective means to collect and classify the factors aggregative with aberrant DNA. Characterization of DNA aggregation-promoting factors We...

Ngày tải lên: 19/02/2014, 06:20

... coenzyme analog lacking the ade- nine ring in the upper axial ligand; a model of damaged cofactors) for free adeninylpentylcobalamin (AdePeCbl) (an inactive coenzyme analog containing the adenine ring ... min )1 at 37 °C from the initial rate. The time course of the reactivation of glycerol-inacti- vated holoenzyme by the reactivase in the presence of ATP and...

Ngày tải lên: 15/02/2014, 01:20

... molecular basis for new therapeutic strate- gies targeting specific pathways to treat human heart disease. Materials and methods Animals Neonatal Wistar rats (1-3 days) were purchased from the Animal ... congestive heart failure. Apoptosis is a highly regulated programme of cell death and can be mediated by death receptors in the plasma membrane, as well as in the mitochondri...

Ngày tải lên: 16/02/2014, 09:20

... respectively, and pcitrine-N1. phPGK1–7aa–cerulean and phPGK1–5aa–cerulean were constructed using the primers 5¢-CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and 5¢-GGCGG ATCCA TAATA TTGCT GAGAG CATCC A- 3¢, and ... 5¢- CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and 5¢-CGACC GGTAT AATAT TGCTG AGAGC AT- CCA-3¢, respectively, and pQE16–hPGK1 as template DNA. After digestion of the...

Ngày tải lên: 16/02/2014, 09:20

... insights into the engineering of DNAzymes. Experimental procedures Materials The DNA sequences (PL DNAzyme, 5¢-GAATTCTAATAC GACTCAGAATGAGTCTGGGCCTCTTTTTAAGAAC-3¢; 8–17 variant DNAzyme, 5¢-AATACTCCGAGCCGGTCG GGCCTC-3¢; ... RNA-cleaving activity in a self-cleaving DNAzyme. The DNAzyme with DNA- cleaving and RNA-cleaving activities was constructed by incorporating the catalytic d...

Ngày tải lên: 16/02/2014, 15:20

... with ROS Cysteine-oxidation and Zn-release rates relatively similar [38] Reaction with Pt compounds Reaction with Cys, Pt bound to Cys Cisplatin and transplatin react faster [36] Cisplatin and transplatin ... domains (a b-domain for the N-terminal cluster and an a- domain for the C-terminal cluster). In the absence of metals, apo-thionein (apo-T) is predominantly unstruc-...

Ngày tải lên: 16/02/2014, 15:20