Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... the chemical inhibitor SB-431542 to block activin ⁄ TGF-b signaling in murine stem cell cultures. Both experi- ments resulted in a strong growth inhibition of the stem cells but, fascinatingly, did not interfere ... neuroepithelial cells into astrocytes, by binding and hyper activating the promoter of glial fibrillary acidic protein (GFAP). In parallel, BMP signals lead to enhanced expre...

Ngày tải lên: 19/02/2014, 00:20

... occur only in initial position in CVC words, certain strings such as NT occur only in final position, while some, such as T, occur in both positions. The initial and final consonant strings of ... the internal-consonant string is an ad- missible initial-consonant string. The words in Class III have the mirror image property that the internal-con- sonant string is an admissi...

Ngày tải lên: 19/02/2014, 19:20

... easily gain access to the RNA protein interface to increase the dissociation process. The promiscuity often reported for proteins M. Doetsch et al. Transient RNA protein interactions in RNA folding FEBS ... through transient interactions with the RNA. Transient RNA protein interactions in RNA folding M. Doetsch et al. 1636 FEBS Journal 278 (2011) 1634–1642 ª...

Ngày tải lên: 14/02/2014, 19:20

... plays a driving role in transient pro- teinÆnucleic acid interactions, as well as in selecting and stabilizing the specific ones. Indeed, it may be the most important factor in the indirect readout ... investing time and effort in preparing and analyzing the full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 a...

Ngày tải lên: 14/02/2014, 19:20

... RNA- cleaving or DNA-cleaving DNAzymes have been obtained by in vitro selection. However, engineering an allosteric DNAzyme with dual RNA- cleaving and DNA-cleaving activities is very challenging. ... applications. In conclusion, we have described a key residue graft- ing strategy for generating RNA- cleaving activity in a self-cleaving DNAzyme. The DNAzyme with DNA- cleaving and RN...

Ngày tải lên: 16/02/2014, 15:20

... effects detected at 96 h in cultures transfected with vectors containing or lacking an insert indicate that after 48 h, other interacting factors could modify the molecular phenotypic effect of using this vector. ... trophozoite stage. In the early stages (3 h), dsRNA-G6PD was highly effective at silencing, decreasing mRNA levels by up to 86%. Nevertheless, this silencing was transient,...

Ngày tải lên: 19/02/2014, 07:20

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... and interleukin-6, and increased hepatic mRNA levels of tumor necrosis factor-a and interleukin-6 were decreased by depletion of KCs. Our findings suggest that ablation of KCs prote...

Ngày tải lên: 14/02/2014, 18:20

... protein kinase; MAPKK, mitogen-activated protein kinase kinase; MBP, myelin basic protein; OXI1, oxidative signal-inducible 1; PDK1, 3-phosphoinositide-dependent kinase 1; PTI1, Pto-interacting ... nuclear EIN3 by bifurcate MAPK cas- cades in C2H4 signalling. Nature 451, 789–795. 36 Benjamins R, Ampudia CS, Hooykaas PJ & Offringa R (2003) PINOID-mediated signaling involves calcium- bind...

Ngày tải lên: 14/02/2014, 19:20

... variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, induction of endoplasmic ... Pharmigen, San Diego, CA, USA) binding and PI staining were performed according to [44]. Following incubation with 1.0 lgÆmL )1 annexin V and 4.0 lgÆmL )1 PI for 20 min, cells were anal...

Ngày tải lên: 14/02/2014, 22:20

... ligand-binding domain; a transmembrane domain; an intracellular tyrosine kinase domain; and a C-terminal regulatory domain [2]. The extracellular domain is subdivided further into four domains. ... EGFR (including EGF, transforming growth factor-a, amphi- regulin and epigen); (b) those that bind to EGFR and ERBB4 (including betacellulin, heparin-binding EGF and epiregulin); and (c) neuregulin...

Ngày tải lên: 16/02/2014, 09:20