Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... 1661 Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe ... S-transferase; HMW, high-molecular- weight; KIND, kinase noncatalytic C-lobe domain; KIND1, first kinase noncatalytic C-lobe...

Ngày tải lên: 14/02/2014, 19:20

... comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present ... nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its quenching within the cells. Right: the various cell lines indi...

Ngày tải lên: 18/02/2014, 13:20

... timing of steps involved in dimer formation and a ppearance of thermostabilization by phosphate has been examined. The role of the cofactor 5¢-phosphate group in the induction of stability and stabil- ization ... [17]. Enzyme–oxyanion interactions occur a t a protein site different from the active site, and thermostabilization is the result of a protein con...

Ngày tải lên: 19/02/2014, 16:20

... IgGFc-binding to the S -domain, and albumin-binding to the C-repeats [31]. Recombinantly expressed fragments rA–S and rS–C3 are indicated. (D) The M1 protein and the A–S and S–C3 fragments of M1 ... protein and protein H appear to be involved in the interaction as the binding of 125 I-labelled DS was reduced to isogenic derivatives of the AP1 strain lac...

Ngày tải lên: 20/02/2014, 11:20

... and its 3D mutant on the kinetics of actin polymerization. Samples containing 1–4 l M of actin (10% of pyrene-labeled protein) were incubated for 5 min in the absence (1) or in the presence of ... The quantity of actin in the pellet was plotted against the total quantity of actin in the sample. Results Heat denaturation of actin Before starting the...

Ngày tải lên: 20/02/2014, 23:20

... yielding the supernatant LS2, containing the cytosol-enriched fraction, and the pellet LP2, which is enriched in synaptic vesicles. The protein concentrations were measured using the bicinchoninic ... sample buffer, and incubated for 30 min at 37 °C. The dithioerythreitol reduces the disulfide bridge in the cross-linker between biotin and the target proteins, a...

Ngày tải lên: 14/02/2014, 21:20

... 1C). Increased nucleolin/C23 protein levels in Hsp70-transfected cells in response to H 2 O 2 -induced apoptosis We next examined the expression of nucleolin ⁄ C23 protein in these cells in response ... by forming large molecular complexes with other factors, such as casein kinase II, c-Myb, midkine, histone H1, nucleophosmin, p53 and protein phosphatase 1. A number of...

Ngày tải lên: 16/02/2014, 09:20

... protease cathepsin D, as indicated by the following observa- tions: (a) the inhibitor profile of the endosomal ETA-degrading activity was very similar to that of cathepsins B and D [30]; and (b) the ... Lipoprotein binding and endosomal itinerary of the low density lipoprotein receptor-related protein in rat liver. Proc Natl Acad Sci USA 86, 931 8–9 322. T. El Hage...

Ngày tải lên: 18/02/2014, 04:20

... loop 2 of ribotox- ins in protein functionality and protein protein and protein lipid interactions has been demonstrated [10,2 9–3 1]. Thus, the first segment of the long loop 2 in a-sarcin has ... of the various groups in determining the dependence of the activity on pH. Comparison with other structures: structural properties of HtA regions involved in...

Ngày tải lên: 18/02/2014, 08:20

... levels continued to increase in virgin queens up to 60 days of age [36]. It was of interest to determine whether recep- tor protein expression paralleled transcript abundance in these virgin queens. The ... via the VgR include: (a) functional VgR translational machinery, which may be negatively regulated by low levels of JH in virgin queens; and (b) the correct locali- z...

Ngày tải lên: 18/02/2014, 08:20