Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... changes in the cytoskeleton that may occur during the initial 6 h window of infection. We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 min and 6 h postinfection, ... play a role in viral spread by mediating cell-to-cell fusion [32,33], we also decided to examine the role of nectin-1 in the fusion of RPE cells with viral glyco...

Ngày tải lên: 18/02/2014, 14:20

... compilation ª 2011 FEBS 2317 Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction Tae-Hoon Kim*, Sang-Ho Lee* and Sun-Mee Lee School of Pharmacy, Sungkyunkwan University, ... factor-a and interleukin-6 were decreased by depletion of KCs. Our findings suggest that ablation of KCs protects against hepatic drug- metabolizing dysfunc...

Ngày tải lên: 14/02/2014, 18:20

... have indi- cated that the CagA PY -induced cell elongation pheno- type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well- known actin-binding proteins ... of distinct EPIYA-independent domains within CagA that play essential roles in protein targeting and alteration of host-cell transcription signalling pathways. Another important conseque...

Ngày tải lên: 14/02/2014, 19:20

... role in skin wound healing. After a skin injury, PPARb expression is rap- idly elevated in the epidermis at the wound edges; dele- tion of a single PPARb allele results in delayed wound healing ... cultured human keratinocytes [14]. In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in a mouse...

Ngày tải lên: 18/02/2014, 18:20

... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits was observed, coincident ... with monensin [5]. In addition, both drugs comparably inhibited generation of the CT-A1 peptide. In rat liver, chloroquine accumulates in hepatic endosomes, leading to an increas...

Ngày tải lên: 19/02/2014, 02:20

... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNA binding, Bcl-X L expression, ... protein-tyrosine kinases in vivo and in vitro. Cell 57, 1109–1122. 18 Holgado-Madruga M, Emlet DR, Moscatello DK, Godwin AK & Wong AJ (1996) A Grb2-associated...

Ngày tải lên: 19/02/2014, 06:20

... not interact with R124 in the ternary complex [20]. Hence, the mechanism of binding of fosfomycin by reinforcement of an initial binding site (mainly K22 and R397) through recruit- ment of secondary ... charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a sing...

Ngày tải lên: 19/02/2014, 13:20

... on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. Some s pecial inhibitors ... Phe53 rings are in both o rientations stacked by p–p interaction with Phe153 rings of the neighbouring protein dimer in crystal. The alternative positions of ph...

Ngày tải lên: 19/02/2014, 16:20

... periods of times (0–60 min) at 37 °C. The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In the absence of ... measuring the binding of [ 125 I]Streptavidin [30]. No Ig binding was observed in cells transfected with the vector alone (Fig. 4B). As shown in Fig. 4B, quantification of cel...

Ngày tải lên: 21/02/2014, 00:20

... unit of enzyme activity was defined as the reduction of 2 lmolÆ- min )1 of metronidazole (corresponding to the decarboxyl- ation of 1 lmolÆmin )1 of pyruvate on the assumption that the bleaching of ... characterization of enzymes that transfer electrons to ferredoxins in vivo is of particular importance for the improvement of this coupled assay and also with respect to obta...

Ngày tải lên: 16/02/2014, 09:20