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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... changes in the cytoskeleton thatmay occur during the initial 6 h window of infection.We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 min and 6 hpostinfection, ... play a role in viralspread by mediating cell-to-cell fusion [32,33], we alsodecided to examine the role of nectin-1 in the fusion of RPE cells with viral glycoprotein-expressing cells. In asemiquantitative, ... HSV-1 infection of all target cells, includ-ing RPE cells, our study provides new starting pointsfor understanding viral pathogenesis in the retina andadvancing novel therapies to control retinal...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... compilation ª 2011 FEBS 2317 Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction Tae-Hoon Kim*, Sang-Ho Lee* and Sun-Mee LeeSchool of Pharmacy, Sungkyunkwan University, ... factor-a and interleukin-6 weredecreased by depletion of KCs. Our findings suggest that ablation of KCsprotects against hepatic drug- metabolizing dysfunction by modulation of the in ammatory ... onabnormalities in microsomal drug- metabolizing func-tion during the late phase of sepsis [6]. However, theunderlying mechanisms involved in hepatic dysfunction during sepsis remain elusive.Among...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin-binding proteins ... of distinctEPIYA-independent domains within CagA that playessential roles in protein targeting and alteration of host-cell transcription signalling pathways.Another important consequence of ... them unsuitable for the investigation of b-cate-nin signalling [29]. In support of this aspect, usingMadin–Darby canine kidney cells that expressE-cadherin and b-catenin without any mutations,...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... role in skin woundhealing. After a skin injury, PPARb expression is rap-idly elevated in the epidermis at the wound edges; dele-tion of a single PPARb allele results in delayed woundhealing ... culturedhuman keratinocytes [14]. In this study, we examined the interaction betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation ... regulation of Cerlevels is involved in enhanced cell survival resultingfrom activation of PPARb.Activation of PPARb by LD induces CERK mRNAexpression and increases CerK activity in SP1 cells To investigate...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf

... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits wasobserved, coincident ... withmonensin [5]. In addition, both drugs comparablyinhibited generation of the CT-A1 peptide. In rat liver,chloroquine accumulates in hepatic endosomes, leadingto an increase in the lag ... have shown here thatexpression of cathepsin D in the cathepsin D-deficient3Y1-Ad12 cell line led to an increase in CT action,whereas siRNA of cathepsin D in MCF-7 cells reducedCT sensitivity....
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNAbinding, Bcl-XLexpression, ... protein-tyrosine kinases in vivo and in vitro. Cell 57, 1109–1122.18 Holgado-Madruga M, Emlet DR, Moscatello DK,Godwin AK & Wong AJ (1996) A Grb2-associateddocking protein in EGF- and insulin-receptor ... of c-Fos, a member of the Fosfamily, AP1 may be implicated in the transcription of bcl-XLinduced by EGF. We then studied the effects of EGF on AP1 DNA binding in TMK-1 cells. The resultsof...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... notinteract with R124 in the ternary complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22 and R397) through recruit-ment of secondary ... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino...
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Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx

Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx

... onconformation of inhibitor main chain. Thus, differentinhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the bindingtunnel. Some s pecial inhibitors ... Phe53 rings are in both o rientationsstacked by p–p interaction with Phe153 rings of theneighbouring protein dimer in crystal. The alternativepositions of phenylalanine rings a re formed in concert ... 271) 4453Inhibitor binding. The inhibitor OE b inds into the p roteasebinding tunnel in an e xtended c onformation in two oppositedirections (Fig. 4). The flaps are locked over the inhibitor...
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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf

Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf

... periods of times (0–60 min)at 37 °C. The concentration of the biotinylated Ig remainingon the cell surface was then quantified by measuring thebinding of [125I]Streptavidin. In the absence of ... measuring thebinding of [125I]Streptavidin [30]. No Ig binding wasobserved in cells transfected with the vector alone (Fig. 4B). As shown in Fig. 4B, quantification of cell surfaceexpression of ... Theywere then incubated with 0.25 mL of incubation mediumcontaining 2 lgÆmL)1 of biotinylated R5T-34 monoclonalIg [30] at 4 °C for 30 min. In some cases, monensin 40 lMwas added to the incubation...
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... unit of enzyme activity was defined as the reduction of 2 lmolÆ-min)1 of metronidazole (corresponding to the decarboxyl-ation of 1 lmolÆmin)1 of pyruvate on the assumption thatthe bleaching of ... characterization of enzymes thattransfer electrons to ferredoxins in vivo is of particularimportance for the improvement of this coupled assayand also with respect to obtaining a deeper under-standing of ... 3A was< 10% that of the [4Fe-4S]1+clusters in Fig. 3B(determined by comparing the double integrals of theEPR spectra), indicating that a substoichiometricamount of the [3Fe-4S] cluster...
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