Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... 407, 39 1–4 12. Mechanisms of glyphosate resistance L. Pollegioni et al. 2766 FEBS Journal 278 (2011) 275 3–2 766 ª 2011 The Authors Journal compilation ª 2011 FEBS REVIEW ARTICLE Molecular basis of glyphosate ... molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of EPSPS EPSPS catalyzes the tra...

Ngày tải lên: 14/02/2014, 14:20

... consists of 10 amino acids, seven of which are conserved within the species (Fig. 2C) and two of which are conserved within all ACAD family of enzymes, forming a highly conserved motif of Gly–Gly–X–Gly. ... encodes a protein homologous to bacte- rial and baculovirus chitinases. Insect Biochem Mol Biol 33, 74 9–7 59. 30 Laemmli UK (1970) Cleavage of structural proteins during a...

Ngày tải lên: 18/02/2014, 04:20

... alignment of the APUM proteins with the PUF domains most similar to Drosophila PUF domain. (A) APUM proteins of group I. (B) APUM proteins of group II. C. W. Francischini and R. B. Quaggio PUF proteins ... a new class of RNA-binding proteins. RNA 3, 142 1–1 433. 35 Cheong CG & Hall TM (2006) Engineering RNA sequence specificity of Pumilio repeats. Proc Natl Acad Sci USA 103,...

Ngày tải lên: 18/02/2014, 06:20

... system with constraints – molecular- dynamics of N-alkanes. J Comput Phys 23, 32 7–3 41. 40 Pastor IWR, Brooks BR & Szabo AJ (1998) An analy- sis of the accuracy of Langevin and molecular- dynamics algorithms. ... computational chemistry approaches to identify the molecular determinants of ligand specificity of CtCBM11. By means of NMR studies, we have analyzed vari...

Ngày tải lên: 18/02/2014, 17:20

... interferon-c-inducible protein- 10; MAPK, mitogen-activated protein kinase; MCP, monocyte chemoattractant protein; Mig, monokine induced by interferon-c; MIP, macrophage inflammatory protein; MMP, matrix metalloproteinase; ... synovial tissues. Chemokines In humans there are more than 50 types of chemokines – small ( 8–1 0 kDa) heparin-binding proteins – that were originally i...

Ngày tải lên: 18/02/2014, 18:20

... 0. 5–1 % of the population. RA causes progressive joint destruction that leads to the restriction of activities of daily living and deterioration of quality of life. Although the pathogenesis of ... disease that affects 0. 5–1 % of the popu- lation and causes progressive joint destruction that leads to the restriction of activities of daily living and deterioration of...

Ngày tải lên: 18/02/2014, 18:20

... activity, such as control of their synthesis or activity, including the inhibition of protein protein interactions in the activating signaling cascade of the transcription factor of interest. Specific ... etc. Glucocorticoids [ Science (1995) 270, 28 6–2 90, Science (1994) 265, 95 6–9 59, Science (1994) 270, 28 3–2 86] [ Mol Cell Biol (1995) 15, 94 3–9 53] Fig. 2. NF-jB activ...

Ngày tải lên: 18/02/2014, 18:20

... o nly the first 30 min of incubation, whereas t hat o f t he human Table 1. Summary of the purification of DNase I from 4 g of pancreas of H. japonicus. Purification step Protein (mg) Total activity (U) Volume (ml) Specific ... the cotranslational protein targeting pathway: hydrophobicity of the central core and t he presence of an a-helical stru cture in the signal s equence r...

Ngày tải lên: 19/02/2014, 16:20

... University of Crete, Department of Biology, Heraklion, Crete, Greece 2 Institute of Molecular Biology and Biotechnology, Heraklion, Crete, Greece 3 Democritus University of Thrace, Department of Molecular ... shown with different shades of gray in the topology diagram of Fig. 1A. The N-ter- minal part of the protein (residues 2–1 49) adopts a Rossmann fold motif. Thi...

Ngày tải lên: 19/02/2014, 00:20

... presumably because of the large number of potential combinations of generated mutants. Fig. 1. Comparison of the structure of 5,6-dihydrofolate (DHF) and trimethoprim (TMP). Of particular interest ... concentrations of TMP in the range of 1 6–3 2 times that of the susceptible form [17]. Some of the DHFR mutants generated in this study were resistant to TMP concentrations...

Ngày tải lên: 19/02/2014, 02:20