I V yiaB tfafi awt affr?ft vwaa Qblltt
t 0^ u^v-'U olil uuc dy i^
":.*^ c i ^ rjtfi^oo troRi; C|tc n*cu .:^nc nhqu (0£*,V07, c hi 270" ^1Z 33X 1<^0*>. ^'Vv^) k h i th'?y c|<3i v l v r l cv^ k i c h t h | c b
d l c u ^hiCn ụ" on? ux'tftfn t b y C'u:'» 1SZ_ 90 270' 79:^ \n 190' > 2SZ u 25'/, 180' •'•'•'•••'•^•'•'> 0 - ^vhi :^lch t h l c h e i ^
k h i e n cT'iC dypt vsio coc
s y i vlbxlr^M^tf p a l o cj»u(ii vo p h i - .; (Cy^ Otfl chJu kx': ., ic'r htfni;(iJ)»
1= + + 270. m.. /0b 3dX wz HLJOJt 970' 28X i \J£2 21190" 160 H2Z b - 4 b l ^i^icr t; l e y ^lt»u khie:^^ Gct=^ OyU; vticr coc ^
J5tfl ^' P'-^ỉ^ crt;.n (i^) ^^
P'QIO i-V'i ^ " i ^ ^ ^ V ^^^^
K i c ; ci;ịCí: r.tfn^.. ( i i ) .
GblSu d n l n y i ten vryn b^
try^ y^n^ tfn.^v5ị^ty I g
so l.jn^u<5 c t r ont j biXit*, *
« 100 «
Xhl k l c b t h l c h dlcu .-tbiln vo kich t h l c h biAie; d§c voo coc syl kboc boâ^, dlni, thtfi kleh thlcb d i l u k h l l n tf pblo t r S n , 5 c chl tbtfflteG ảniEt bl^n GTODG btftfne ^° ( c h i l e 54> >* tlof;; so lan tfc
chl acuat blgn) vo htftfCi;, SÔ (chllm 51 ,. cln,^ no l l n 5c ebl
s u i t h l g n ) . linage I g i , nlu kich tulctidiSu khlln tf pbla dtftfl k i c b t b l c b btfn«^, chl uc c h l x u l t b l | n nbllu tf htf&ig 14*0° (chllm 42 -^ tlnrjiol l l n tfc chl x u l t hl^u) vn btftfnt^ --70^ ( c b l l a 28 >
tcmi s i iSn 5c c h l x u l t bl§n)# íutfnc, QUon gltfo coe hi/tfnc^ tfc
che vo v i t r l kleu t n l c h d i l u khlln ( b l l u uhj tlxio ty e l >> ) troot; trtftfn^: bffp uoy dtfj^ mlnb boo t r e n blnh 59b#
libtf vgy 0 d p i da s i ntfron dtftfl onb biiitfng cua klob t b l e b d l l u kblln ctftfUt, tSe &c c h l x u l t bl^n tf btftfu^T ngt.'sfe v l ^ cbleu
cuo kich t h l c h dJeu kblen* Dllu do cuce:; co cghlo lo btftfng nbpy coEfl chlnh cuo ntfron trunt, vtfl btftfng vo t r l n h ctf ngbiSiâ
cuo coc ss^'l chj^u k l c n t b l c h d i l u khl«jn vo ^Icb t d l c h btfnt*
Ooc k i t quo triln dfiy cho pbep chunt\ t o l ne;bl C^n tlnb
c h i t ebun^; cua coc ntfron n ^ I^HQ nhou t cnc ntfron naa (^l^n nbou
CO btftfng nb^y câ chun£^ ctftfl anb btftfng: cuo kich titlcb t|? nbl@n« s l n b CbIt nay glup cbo coc ntfron co kha naofj: cur<; t l l p nb§tt
101 «
TAG DUNG CUA PICKOTOXIK DOI VOI TEUOfiG TW CÂ
VA SU BIEK DOI TUm hhAl CAki Tiifc,0 HUÔG CVA WOKOH
»btf t r o n g pbSn I I ebuna t o l dZ t r l n b boy, coc a l c o l o l t
b l c u c u l l n vo p i c r o t o x i n (coc c h i t del kbang cua GAB4) co toe
dyng IsBJ giom anb btftfot; tfc cbe cuo c h i t trung gian hoo hpc
va do do lam tbay d c i coc d§c t l n b cua coc ntfron trong vo bsn c l u d^i noọ
Mhtfutj van de d^t ro di ngbiSn ctfu tron^; cbtftfng noy nblffi tlffi bieu onh btftfot^ cuo p i c r o t o x i n d l i vtfl trtftfng tby
caro cua ntfron vung c b l l u thuyc ctf quan nh|n cam v i b r i s s va
d l i Vtfi sg* bien d l i t l n b nh^iy cm theo biftfnc;; dtf^c blnh thonh
dtftfl toe dyni cua tfc cbl d l l u khiSn btftfng oara, Qun do co t b l bleu dtftfc ban c h i t cuo tfc c h l biitfu^; cam va vol t r o eug no d l i Vtfl s|/ hlnh tbanb cae d§ic t i n h cuo nôon. Srong cbtftfng nay chun<;:: ti^i t l e n bonh ph^n t l c h k i t quo nghien ctfu dtfqfc tren 25 ntfron, tron^:' dS co ^ ntfron n£;uyeD pbot ^CÍH,
I / - i^AO DU^G MO jUCaSG TkihOịi, ThU GAM ChA PICKOIOZl^'
1)91 VOI QAO maoh VUJ^-JG GHIJ U THUQU CO QU^U
hilMi QAii^ g'G CAG SOl VUiJiXUS
Blnb thtftfog, kbl cbtfa co t a c dyn^ cun p i c r o t o x i n trtftfng
thy COO) tf phan l 5 n cae ntfron boo r>um kbogng 2 cin 5 B0.
v i b r i s s . Tuy nblen cbun^^ t d i cung do g|ip 6 ntfron co trtftfng tby coa kba 3?<?ngt chiJa t r S n 10 sj^i v i b r i s s * i^btfng sg^l noy dIu tf ©yt ben pblo d l i dlgn vtfi vun^ vo noo dújyc gbl dign t h l ntfron.
Kbi kleh t b l c b cae sg^l kboc nbau nam trong: trtftfng tby com coc otfron d i p tfng vtfi c^rtfn^ dy vo tbtfU;;ion tiem tgng kboc nbau, Dieu noy do dtf^^ t r l n h boy trong cac chtftfni, t r § n • Qba
B 1 0 2 «
cac cbtftfng nay cbune to t h I y r o r a n g , d l l vtfl coc s ^ kbdng nao tront; trironc tby coa, t b l kbl klcb t h l c h cbun^;, bo§c
kbdng gay onb btftfn^v^-1 din hôt d$ng phot xun^ cua coc ntfron,
hôc c b l l a s thoy d l l dlgn t h l t a i chd.
Cac k i t quo n/:^hien ctfu tronp; pbon nSy cbo thIy sou t a c d§np cuo p i c r o t o x i n 5 pbut (tbtfl gion doô m^jcb dong vi d i | n
dl lô5-5 p h u t , c t J ^ : dy i;run£ blnh cua dong ll 50 nA),trtfSng
thy cam eua t i t ca cac ntfron dIu Eijtf ryng r o r y t .
Cy the cbo tbay t
- D I I Vtfl cae ntfron trtftfc do co trtftfng thy cora c h l glo
2 den 5 s y l v i b r i s s , dtf5l anb b{f0n^ cue don^ vl dlyn d l
p i c r o t o x i n trtftfu^;, tby ooui cuo cbung atf r$Sng va gloj blu b i t
tac CO coe stfi nam tf b§n pblo d l l dijn vo đl khi con l a o
ryng ra den c y t v a i vunt: nbo t r e n do oyt vo ê»
- Doi vtfi Cac ntfron trtftfc day co crtftfn(^: thy cam ttftfng d l i ryng (gSm t r e n 10 s y l vlbrlGs)sou tac dyng coa dong v l
dlfn dl p i c r o t o x i n trtftfrc- tby cam boo i^m khong M»l c h l r l e n g cac s9^l b§n phla cJol dlyn, Bo con lan r$D,j gen bet vung myt
va c c . Dye b i ^ t tf hoi ntfron trtftfu^^ thy caD dn rotf ryng sang vun£, tfl^t cung ben. Kleh t h l c h cae sýi v l b r l a s cung- b§n ntfron da t r o I t f l , tuy etftfc^:. dy eua pbon tfn^; ylu btfn vo tbtfl glon tlScD tong d a l btfn so v5l phan tfng kbl klcb thlch cae s??l ben 4I1 d i ^ n .
ảr§o blnb ^ (pban occllograom tf dtf5l) mlnb bya i^t
trong nbtfng tirtftfne: hyjJ.'^ttur COB cua ntfron dtf^c srtf rj^ng dtftfl anh btftfng cua dong v l dlyn dl p l c r o t D x l n .
Blnb tbtftfn^:, lelcb t h l c h d l l u k b i l o (C) chl gfiy b i l n d l l dlfn t h l t p l chd cbtfa din rotfc laEi cho ntfron phat xung
(OeellograrotD!' c y t 2 ) . Sou t a c d^Jog cuo p i c r o t o x i n ntfron khdng nbimg t a n g dop tfnc: d e l vtfi klcb t h l c h biJng (A)f oa con dop^ t/n^; 1^1 k i c h chlcb d l l u k b l l n bang coc chuio xuns(oecllogracjm tf <$t 5 ) • Qua ^0 phut sau k b l ngiJne dong vi dign d l p i c r o t o x i n .
a 103 3
dap tfne cuântfron del vtfl k i c h thlch dieu k h i l n vo kleh t h l c h hSng^lpi trtf ve otfc c u . Kich thlch d l l u k h l l n kbdn^v gSy phon tfng tf ntfron nlio (occilogromtria tf cyt ^ ) ,
Kbtf v§y p i c r o t o x i n do l a o mlt gob htftfnp; tfc chl olo GABA
va do do mtf r^n^: trtftfnt; tby cnm cuo ntfron tbu^ic vvn{^ c h l l u ctf
quon nbyn cod ttf cac s y l v i b r i s s .
1 1 / - 'rAC Dum C\XH irichQi:oxm BOI VOI SU BIIJ; UOI
De t l o hieu ay b i l n đi t i n h nhpy com theo btftfng cua ntfron dtftfl onh btftfuf; cua picroi;o>:ln chtftfnc t r l n h t h l ngbifai cuo chuari t u l cuo fsdi ntfron nhif sau ;
- Gbl dap im^ cuo rjôron doi vol coc kich thlch rlSng l e (si^i A) tront bin hiftfn^ ctf ban (cOt 1 cua do t h l ) .
- Tien hanh ghi dap tfnc eua noT-on doi vtri coc kleh t h l c h đi (B-A hoyc C-A) VO phan t l c h syr xuat hi§n t i n h nhpy coo theo btftfng, cua ntfron trong dieu kign nol tren (cyu 2 ) .
- Cbo donfj; vi difn di p i c r o t o x i n cnc d^nii, len ntfron.
Thtfi g i a n donp; oych io 5-5 pbut. Gtftfng dj? cua dong ctf 5^ den
90 n 4 ( (tbtftfn^ l o 50 nk).
- ^goy sau kbl nf-tfni dong vl difn d i , thtf l a i onb btftfng cu3 kich t h l c h đl va theo doi stf tboy dSi t l n b nbay co«8 theo htftfnp; cuo ntfron ( c g t 3 ) .
- Itboong 50-^0 pbut k l ttf luc nctfnt: uoc dynt: cuo dong v l i l f n d i , t l l n bonh k i c h t b l c h đl t l l p . qual^at stf b l l phyc cuo t i n h nhpy cam theo btftfrn:; ( c y t 4 ) .
Kit qua t h l nLhiym cho thIy p i c r o c o x i n do lam mat txnb Dhgy caK theo htftfnG tf nóron UfjUyen phot fcCTH vo eo tf coc ntfron nguíen phat KI^CTH, nhtfng ulnb c h i t nay da dtfýc hinh th^nh t r o n g qua t r i n h kich chich d d i .
104
^ Dtftfl d§y c h i n g t d i l l n Itf^t t r l n h bay c | e k i t qua ngfaifiii Ctfu dtfyc t r o n g l o ^ t t h l nghigto n a y .
! • gPt. V0i cnc ntfron n^-uyen phnfc liC^R.
Dtftfl anh biftfng cue k l e h t h l c h d d l cnunt, t d l unl^ 3 t r o n g s i 4 ntfron c o t i n h nh^y cao trtf nen r o n e t ban, r i e n g d l l vtfi ntfron thtf ttf eo stf t h a y d d l I p i t i n h nhyy cam t b e o btftfng.
Sau k h i cho done v l d l f n dl p i c r o t o x i n t a c dyng t i t co