2.1.1 Collection and Preprocessing of Clinical Data
2.1.1.4 Summary of Clinical and Immunological Data used for Analyses
Table 2.1: Overview of generated tables which were eventually used for analyses. 1=1st visit; 2=2nd visit; 3=3rd visit.
Table Name Cases Information included Description
EDEN_TOTAL 453 Clinical Data 1
DENGUE_TOTAL 133 Clinical Data 1, 2, 3
SEVERITY_TOTAL 125 Clinical Data 1, 2, 3 PLT_CLASS (low/high) EDEN_IMMUN_TOTAL 291 Clinical & Cytokine Data 1
DENGUE_IMMUN_TOTAL 95 Clinical & Cytokine Data 1, 2, 3
SEVERITY_IMMUN_TOTAL 89 Clinical & Cytokine Data 1, 2, 3 PLT_CLASS (low/high)
Table 2.2: Overview and explanation of the immunological parameters used for analyses. 1=1st visit;
2=2nd visit; 3=3rd visit.
Abbreviation Unit Explanation
IP_10 1,2,3 [pg/ml] Interferon γ inducible protein 10 (IP-10)
I_TAC 1,2,3 [pg/ml] Interferon inducible T cell α chemoattarctant (I-TAC) IFN_ALPHA 1,2,3 [pg/ml] Interferon α (IFN-α)
GM_CSF 1,2,3 [pg/ml] Granulocyte macrophage colony-stimulating factor (GM- CSF)
IFN_GAMMA 1,2,3 [pg/ml] Interferon-γ (IFN- γ)
IL_1 1,2,3 [pg/ml] Interleukin-1 (IL-1) IL_10 1,2,3 [pg/ml] Interleukin-10 (IL-10) IL_12 1,2,3 [pg/ml] Interleukin-12 (IL-12) IL_2 1,2,3 [pg/ml] Interleukin-2 (IL-2) IL_4 1,2,3 [pg/ml] Interleukin-4 (IL-4) IL_6 1,2,3 [pg/ml] Interleukin-6 (IL-6) IL_8 1,2,3 [pg/ml] Interleukin-8 (IL-8)
TNF 1,2,3 [pg/ml] Tumor necrosis factor α (TNF- α)
Table 2.3: Overview and explanation of the clinical parameters used for analyses. 1=1st visit; 2=2nd visit; 3=3rd visit (NIH, 2007).
Abbreviation Unit Explanation
ID None Sample identification number
TEMP 1,2 °C Body temperature
PULSERATE 1,2 Beats per
minute Pulse rate
SYSTOLICBP 1,2 mmHg Systolic blood pressure DIASTOLICBP 1,2 mmHg Diastolic blood pressure
WBC 1,2,3 Cells/microliter
[x103]
White blood cell count. It is almost always part of the CBC (complete blood count); Normal count is between 4,500- 10,000 white blood cells/àl.
RBC 1,2,3 Cells/microliter
[x106]
Red Blood Cell count (varies with altitude):
Male: 4.7 to 6.1 million cells/àl; Female: 4.2 to 5.4 million cells/àl
HGB 1,2,3 Grams/deciliter Hemoglobin (varies with altitude):
Male: 13.8 to 17.2 g/dL; Female: 12.1 to 15.1 g/dL
HCT 1,2,3 Percentage [%]
The hematocrit is the percent of whole blood that is composed of red blood cells. The hematocrit is a measure of both the number of red blood cells and the size of red blood cells; (varies with altitude):
Male: 40.7 to 50.3 %; Female: 36.1 to 44.3 %
MCV 1,2,3 Femtoliter MCV is the mean corpuscular volume or the average size
of the red blood cells: Normal MCV: 80 to 100 femtoliter.
MCH 1,2,3 Picograms/cell
MCH is the mean corpuscular hemoglobin or the hemoglobin amount per red blood cell (MCH = HGB/RBC).
Normal MCH: MCH: 27 to 31 picograms/cell
MCHC 1,2,3 Grams/deciliter
MCHC is the mean corpuscular hemoglobin concentration or the hemoglobin concentration (hemoglobin amount relative to the size of the cell) per red blood cell (HGB/HCT).
Normal MCHC: 32 to 36 grams/deciliter
PLT 1,2,3 Number/microli
ter [x103]
PLT is the Platelet count or thrombocyte count.
Normal PLT: 150,000 to 400,000/àl
LYMPH_PCT 1,2,3 Percentage [%] The percentage of the WBC belonging to lymphocytes.
Normal LYMPH_PCT: 20% to 40% of the WBC
MXD_PCT 1,2,3 Percentage [%]
The percentage of the WBC belonging to monocytes, eosinophiles & basophiles.
Monocytes: 2%-8%; Eosinophils: 1%-4%; Basophils:
0.5%-1%
NEUT_PCT 1,2,3 Percentage [%] The percentage of the WBC belonging to neutrophiles.
Normal NEUT_PCT: 40% to 60% of the WBC LYMPH_NO 1,2,3 Cells/microliter
[x103]
The absolute number of lymphocytes calculated by multiplying LYMPH_PCT with WBC. (LYMPH_PCT x WBC)
Table 2.3 (continued): Overview and explanation of the clinical parameters used. 1=1st visit; 2=2nd visit; 3=3rd visit.
Abbreviation Unit Explanation MXD_NO 1,2,3 Cells/microliter
[x103]
The absolute number of monocytes, eosinophiles &
basophiles calculated by multiplying MXD_PCT with WBC. (MXD_PCT x WBC)
NEUT_NO 1,2,3 Cells/microliter [x103]
The absolute number of neutrophiles calculated by multiplying NEUT_PCT with WBC. (NEUT_PCT x WBC)
RDW_CV 1,2,3 Percentage [%]
Red blood cell distribution width is a measure of the variation of red blood cell volume that is reported as part of a standard complete blood count. Usually red blood cells are a standard size. It’s a coeffiecient of variation ((Standard deviation of red cell volume/mean cell volume)
× 100); Normal range: 11.5-14.5%
RDW_SD 1,2,3 Percentage [%] Standard deviation of RDW_CV.
PDW 1,2,3 Femtoliter Platelet distribution width.
MPV 1,2,3 Femtoliter Mean platelet volume is a machine calculated
measurement of the average size of the platelets.
P_LCR_PCT 1,2,3 Percentage [%] Platelet Large Cell Ratio
DV_PCR 1 None Detection of dengue Virus by PCR. (positive/negative) DV_IG_G 1 None Detection of dengue virus antibodies: marker for secondary
infection. (positive=secondary/negative=primary)
CT_COLLECTION 1 Number of cycles
The Ct-value is the number of cycles used to reach a specific threshold in the RT-PCR. High Ct-value means a low amount of template and a low Ct-value represents a large amount of template.
HOSPITALIZATION None Hospitalization status of dengue positive patients (yes/no).
PLT_CLASS None Severity classification of patients defined with regard to the platelet count (low/high); (For more details see Results;
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