Tác giả tiếp tục phát triển các mô hình đa kênh dẫn microfluidic kết hợp sử dụng hệ các ống dẫn nano thay cho màng lựa chọn ion để gia tăng và điều khiển sự tập trung nồng độ phân tử DNA nói riêng và các phân tử/ tế bào sinh học nói chung. Sử dụng kết hợp phương pháp Lagrangian và phương pháp biên nhúng để mô hình hóa các phân tử sinh học, sự biến dạng và chuyển động của chúng trong hệ điện hóa.
31 TÀI LIỆU THAM KHẢO
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33 PHỤ LỤC
A1. Các bài báo đã công bố
[1] V. B. Nguyen and V. S. Pham, “Study and modeling DNA-preconcentration microfluidic device,” J. Sci. Technol., vol. 143, pp. 001-006, 2020.
[2] Q. V. Do, V. B. Nguyen, P. K. Nguyen, and V. S. Pham, “An Immersed Boundary Method OpenFOAM Solver for Structure – Two-phase Flow Interaction,” J. Sci. Technol., vol. 138, pp. 028–032, 2019.
[3] V. B. Nguyen, Q. V. Do, and V. S. Pham, “An OpenFOAM solver for multiphase and turbulent flow,” Phys. Fluids, vol. 32, no. 4, p. 043303, 2020.
[4] Viet Bac Nguyen and Van Sang Pham, Develop immersed boundary method OpenFOAM solver for multiphase and turbulent flow, The 30th International Symposium on Transport Phenomena conference, 2019.
[5] Pham Van Sang and Nguyen Viet Bac "A Study on Ions Transports Through Charged Nanopores" International Conference on Fluid Machinery and Automation Systems - ICFMAS2018, Hanoi, Vietnam, 2018.
A2. Tham số điều kiện biên
// Khai báo thông tin về tên lưới, tên các biên, và thuộc tính vật lí của các biên.
Grid {
filename = StudyCases/JST2020/mesh/flatMem_5_2_2.msh;
inlet = [inlet]; inlet dummy = [inlet dummy];
outlet = [outlet]; outlet dummy = [outlet dummy];
membrane = [membrane1,membrane2]; membrane dummy = [membrane dummy];
electrode = [electrode]; electrode dummy = [electrode dummy];
symmetry = [symmetry]; symmetry dummy = [symmetry dummy];
wall = [wallLeft,wallRight]; wall dummy = [wallRight dummy];
empty = [empty];
periodic = [periodic]; periodic shadow = [periodicshadow];
}
// Khai báo giá trị các đại lượng không thứ nguyên.
Parameter {
faraday constant = 96485.3415;
length scale = 20E-6;// in meter concentration scale= 10;//mol/m3 velocity scale = 1;//m/sec
temp = 300.0;
wall surface charge = 0.0065;//C/m2 average diffusivity = 1E-9;
34 charge number = 1;
stokes critical = -10;
dynamic viscosity=8.9E-4;//pas.sec g = [0.0,0.0,-9.8];
}
// Khai báo giá trị khởi tạo, thuộc tính của các biến Variable_1
{
name = Na+;
type = ion;
unit = mol/m3;
diffusivity = 1.33E-9;
chargenumber = 1;
initial value = 1;
boundary_1(name = inlet; value = 1;type = fixedvalue;);
boundary_2(name = outlet; value = 1;type = fixedvalue;);
boundary_3(name = wallLeft; value = 0;type = fixedionicflux;);
boundary_4(name = wallRight; value = 0;type = fixedionicflux;);
boundary_5(name = membrane1; value = 1.5;type = fixedvalue;);
boundary_6(name = membrane2; value = 1.5;type = fixedvalue;);
}
Variable_2 {
name = Cl-;
type = ion;
unit = mol/m3;
diffusivity = 2.03E-9;
chargenumber = -1;
initial value = 1;
boundary_1(name = inlet; value = 1;type = fixedvalue;);
boundary_2(name = outlet; value = 1;type = fixedvalue;);
boundary_3(name = wallLeft; value = 0;type = fixedionicflux;);
boundary_4(name = wallRight; value = 0;type = fixedionicflux;);
boundary_5(name = membrane1; value = 0;type = fixedionicflux;);
boundary_6(name = membrane2; value = 0;type = fixedionicflux;);
}
Variable_3 {
name = Phi;
type = potential;
35 unit = Vol;
initial value = 0;
boundary_1(name = inlet; value = 35;type = fixedvalue;);
boundary_2(name = outlet; value = 0;type = fixedvalue;);
boundary_3(name = wallLeft; value = 0;type = fixedgradient;);
boundary_4(name = wallRight; value = 0;type = fixedgradient;);
boundary_5(name = membrane1; value = 0;type = fixedvalue;);
boundary_6(name = membrane2; value = 0;type = fixedvalue;);
}
Variable_4 {
name = U;
type = velocity;
unit = m/s;
initial value = 0;
boundary_1(name = inlet; value = 0;type = fixedgradient;);
boundary_2(name = outlet; value = 0;type = fixedgradient;);
boundary_3(name = wallLeft; value = 0;type = fixedvalue;);
boundary_4(name = wallRight; value = 0;type = fixedvalue;);
boundary_5(name = membrane1; value = 0;type = fixedvalue;);
boundary_6(name = membrane2; value = 0;type = fixedvalue;);
}
Variable_5 {
name = V;
type = velocity;
unit = m/s;
initial value = 0;
boundary_1(name = inlet; value = 0;type = fixedgradient;);
boundary_2(name = outlet; value = 0;type = fixedgradient;);
boundary_3(name = wallLeft; value = 0;type = fixedvalue;);
boundary_4(name = wallRight; value = 0;type = fixedvalue;);
boundary_5(name = membrane1; value = 0;type = fixedvalue;);
boundary_6(name = membrane2; value = 0;type = fixedvalue;);
}
Variable_6 {
name = Pre;
type = pressure;
36 unit = N/m2;
initial value = 0;
boundary_1(name = inlet; value = 0;type = fixedgradient;);
boundary_2(name = outlet; value = 0;type = fixedvalue;);
boundary_3(name = wallLeft; value = 0;type = fixedgradient;);
boundary_4(name = wallRight; value = 0;type = fixedgradient;);
boundary_5(name = membrane1; value = 0;type = fixedgradient;);
boundary_6(name = membrane2; value = 0;type = fixedgradient;);
}
Variable_7 {
name = DNA;
type = dna;
unit = mol/m3;
diffusivity = 0.0454E-9;
chargenumber = -50;
initial value = 0.1;
boundary_1(name = inlet; value = 0;type = fixedgradient;);
boundary_2(name = outlet; value = 0;type = fixedgradient;);
boundary_3(name = wallLeft; value = 0;type = fixedvalue;);
boundary_4(name = wallRight; value = 0;type = fixedvalue;);
boundary_5(name = membrane1; value = 0;type = fixedvalue;);
boundary_6(name = membrane2; value = 0;type = fixedvalue;);
}
Equation {
name = PNPNS;
make staggered grid = 0;
variables = [Na+,Cl-,Phi,U,V,Pre];
dynamic boundary condition(variable = Phi;type =
potential/*current*/;boundary = [inlet];first value = 35;last value = 40; increment = 1;);
time(timeend = 0.15; timestep = 0.0001;);
time dependent = yes;
dimensionless output = yes;
inlet boundary name = inlet;
outlet boundary name = outlet;
} Solver {