The most severe lesions caused’ by strains N..ipomoeae LNS and F. miscanthi KT212 were
estimated for 25% of the leaf surface (equivalent to infection level 5). At this stage, whole leaves
wilted and fell out.
Ay Editorial System
_ Plant Protection Research ˆ
Table 1. Leaf-associated diseases on Panax ginseng and the causal pathogens
Disease Symptoms Panax ginseng Reported The causal Reference variety planting pathogen
Alternaria Necrotic lesions of Panax America Alternaria Putnam and Toit,area
blight 2 to 20 mm in quinquefolium L. Canada panax 2003;
diameter with a Hill and Háubeck, dark brown 2009.
margin and a
ellow-green halo
Leaf spot Brown oval/round Panax ginseng China Alternaria Lee et al. 2019;
spots of 3to9mm Meyer alternata Wang et al. 2024 in diameter with Boeremia
white/yellow exigua
halos in the center, Panax japonicus China Boeremia You et al, 201 = : leading to leaf exigua ;
withering and Panax America Pestalotiopsis Civanparanage `falling, and plant —_guinguefolius nanjingensis wilting.
Anthracnose Dark spotlesions | Panax ginseng
(might be with a Meyer
hole in the center) Panax ginseng that can be Meyer
enlarged and fused ~Panax
to make blight quinquefolium L.
areas, leading to defoliation
Water-soaked,
concentric lesions
start at the leaf Panax ginseng ˆ _ €
tipsandproceed Meyer ' '“ ..
back along the leaf Se để j mid-rib, resulting
in enlarged blight
zones.
a al., 2004
, Mc.Partland, Hosoya, 1998;
Guan et al., 2021b
Grey mold tàu cinerea
* Botrytis cinerea Wang et al., 2022
Foliar soft Irregular water- ˆ' Pang ⁄ ằ `ằ Vietnam Neocosmospora This study
rot soaked rotten < ~ ‘vietnamensis Cc ipomoeae
lesions of 5 to 1 ; < Fusarium mm in diameter. ` miscanthi
As the disease
progressed, the ..
lesions enlarged ˆ (up to 25% leaf surface), and the `
infected leayes ˆ
turned wilting
black, easily
ara
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Plant Protection Research
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nNo
= °
Quang Nam
- Kon Tum Average disease incidence (%) ° Foliar rot Anthracnose disease disease
Quang Nam - Kon Tum Lai Chau - Lao Cai
Fig. 1: Survey areas (A) and disease incidence (B) of two main foliar diseases detected on
Panax vietnamensis. bồ we 5
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System
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Fig. 2: Symptoms of the foliar soft rot disease (A — on Ngoc Linh variety; C — on Lai Chau variety) and anthracnose disease (B — on Ngoc Linh variety; D — on Lai Chau variety) on Panax vietnamensis.
_ Figure 3ˆ eee tin : ee Le
‘Bownlead source fle (25.82 MB) Plant Protection Research -
LN5-12d
Fig. 3: Infection tests to prove the pathogenicity of the two isolates LN5 and KT2.1.2. A-D
~The first round performed on ginseng leaves in a microclimate chamber; F-l — The second round performed on 2-year-old ginseng plants in a net house. E, K — Negative controls . (ginseng leaves or plants treated with a sterile medium only). Lesions are pointed by arrows.
FB EditorialSystem
Neocosmospora variasi
(NR 177139.1)
(MWS534061.1)
tonkinensis
‘Neocosmospora paraeumartil
(MG189941.1) cara ey (MW534059.1)
ioetran eee Ti &
falciformis
(KT313633.1)
‘Neocosmospora )
(NR 154227.1)
'Neocosmospora solani
crassa
(AB674261.1)
(GU170647.1) J170643.1
{vis )
'N@OCosmospora ipomoese (JX435206.1) (AB426286.1)
% || LNS (PP422928.1)
vasinfocta
(JQ265758,1)
Neocosmospore ipomoeee. 5 (LR583718.1) tenuicristata Laan at ()Q285761.1)
(HM534894.1) 'Allernaria ventricosa
Bối vã (MF070251.1) „001
Fig. 4: Morphology and taxonomic position of strain LN5. A — Colony on PDA; B = Colony, on LCA; C — Septate mycelia; D — Unbranched conidiophore; E — Macro- and.microco idia.
Scale bars 10 um. F, G, H — Phylogenetic trees based on comparative analyses f the ITS, TEF1, and BT2 sequences, respectively (assession numbers f theses ences aree:'sequ
indicated in parentheses next to the strain name).
ti hi PEERS
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Picard
Fusarium oxysporum s „ (MW594320.1)(KP132221.1) _ N1. ng 50 (Mz921886.1)
Fusarium oxysporum |. sp. batates Fuserion dúoseptaTim:° Hi855101.1) = (MW594327.1)
Fusarlum curvatum 100 Fuserium curvatum
(MZ890510.1) (MZ921863.1) Fusarium odoratissimum 98 Fusarium odoratissimum
(MW016597.1) (MW894352.1)
Fusarium nirenbergiae Fusarium commune
(MW016588.1) Cy) (MW620162.1)
KT2.1.2 (PP422929.1) KT2.1.2 (PP458366.1) Fusarium miscanthi Fusarium paranisikadol
2 : (MW016565.1) (MW594317.1)
Fusarium nisikadoi Fusarium miscanthi
83 (MH862661.1) (MW594318.1)Fusarium paranisikadoi Fusarium hipposidericola
(MW0165631) (OR025993.1)
-Nectria dematiosa ôi
cạn) (HQ728152.1)
H Fusarium fabacearum
(OM960818.1) Fusarium acutatum
a (OM960822.1)
Fusarium duoseptatum (OM960817.1) Fusarium elaeidis
(MZ325283.1)
(LC171239.1) (OP901506.1) KT2.1.2
001
Fig. 5: Morphology and taxonomic position of strain KT2.1.2. A = Colony’r ơn PDA: B- Colony
on LCA; C — Septate mycelia; D — Unbranched conidiophore; E o-.and microconidia.
Scale bars: 10 um. F, G, H— Phylogenetic trees based on comparative analyses of the ITS,
TEF1, and BT2 sequences, respectively (assession numbers of these sequences are
indicated in parentheses next to the strain name)
Editorial
8 svete
Fig. S1: Disease index shown by the fungal pathogens Neocosmospora ipomoeae LNS (AD) and Fusarium miscanthi KT212 (E-H) on 2-year-old ginseng plants under net house
conditions. A, E — Beginning of the infection; B, F — Disease level 1;.C, Fe Disease jevel 3;
D, H— Disease level 5.
ẹ Editorial SAL
Tin : : ứt :
Index - hp Đội dc
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Manuscript body
Download source file (81.64 kB) Tables
Table 1 - Download source file (37.95 kB)
Table 2 - Download source file (39.22 kB)
Table 3 - Download source file (17.8 kB)
Figures
Figure 1 - Download source file (91 MB)
Fig. 1: Survey areas (A) and disease incidence (B) of two main foliar diseases detected
on Panax vietnamensis.
Figure 2 - Download source file (31.93 MB)
Fig. 2: Symptoms of the foliar soft rot disease (A — on Ngoc Linh variety; C — on Lai Chau variety) and anthracnose disease (B — on Ngoc Linh variety; D — on Lai Chau variety) on
Panax vietnamensis.
Figure 3 - Download source file (23.82 MB) om
Fig. 3: Infection tests to prove the pathogenicity of the two isolates LN5 and KT2.1.2. A-D ˆ
~The first round performed on ginseng leaves in a microclimate chamber; F-è —Theằ<_—
second round performed on 2-year-old ginseng plants in a net house..E, K Negative
controls (ginseng leaves or plants treated with a sterile medium only). Lesions are pointed
by arrows. — me ì
Figure 4 - Download source file (51.82 MB) eS = ge Fig. 4: Morphology and taxonomic position of strain LN5. A— Colony on PDA; B — Colony on LCA; C — Septate mycelia; D — Unbranched conidiophore; E —Macro- and
microconidia. Scale bars 10 um. F, G, H— Phylogenetic trees based on comparative analyses of the ITS, TEF1, and BT2 sequences, respectively (assession numbers of
these sequences are indicated in parentheses next to the strain name).
Figure 5 - Download source file (50.08 MB) "ý.
Fig. 5: Morphology and taxonomic position of strain KT2.1.2. A— Colony on PDA; B- Colony on LCA; C — Septate mycelia; D — Unbranched conidiophore; E — Macro- and
microconidia. Scale bars: 10:um. F, G; H —- Phylogenetic trees based on comparative
analyses of the ITS, TEF1, and BT2 sequences, respectively (assession numbers of
these sequences are indicated in parentheses next to the strain name).
Figure 6 - Download 'source file (20.01 MB)
Fig. S1: Disease index shown by the fungal pathogens Neocosmospora ipomoeae LNS
(A-D) and Fusarium miscanthi KT212 (E-H) on 2-year-old ginseng plants under net house
conditions. A, E — Beginning of the infection; B, F — Disease level 1; C, G — Disease level
3; D, H— Disease level 5.
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Kết quả nghiên cứu Khoa học BVTV - Số 3/2024
bronzing: a new disease affecting jackfruit caused by Pantoea sfewarti (Smith) Mergaert et al. Annals of
Tropical Research, 36(1), 17-31.
5. Ham, H., Oh, G. R., Park, D. S., & Lee, Y. H.
(2022). Survey of oxolinic acid - resistant Erwinia amylovora in Korean apple and pear orchards, and the fitness impact of constructed mutants. The Plant Pathology Journal, 38(5), 482.
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Hakiman, D. S. Karam, and D. Zulperi. 2020. Draft
genome sequencing data of a pathogenic Pantoea
stewartii subspecies stewarti strain SQT1 causing
bronzing disease of jackfruit in Malaysia. Data Brief.
30:105634.
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10. Võ Thị Ngọc Hà, Phạm Kim Huyền, Đinh Thị Thảo Quyên (2023). Hiện trạng và tác nhân gây bệnh
đen xơ trên mít thái (artocarpus heterophyllus lam.) tại
thành phố Hồ Chí Minh. Trường Đại học Nông Lâm TP. Hồ Chi Minh.
11. Zhao, Y., Xiao, M., Yan, C., Hu, F. C., Zhang, S., Wang, X., & Fan, H. Y. (2023). First Report of Bronzing Disease Caused by Pantoea sfewarii on Jackfruit in China. Plant Dis.
12. ZhaoJunior, J. C. R., Tamanini, R., Soares, B. F., De Oliveira, A. M., De Godoi Silva, F., Da Silva, F. F., & Beloti, V. (2016). Efficiency of boiling and four other methods for genomic DNA extraction of deteriorating spore-forming
bacteria from milk. Semina: Ciéncias
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Phan bién: PGS.TS. Nguyén Van Vién