mjƒpi"jw{院v"vjcpj"mjƒpi"p丑e.
A吋"vtwpi"j”c"jq衣v"v pj"zw医v"jw{院v"e栄c"p丑ẹ"jw{院v"vjcpj"mjƒpi"p丑e"vj逢運pi"8逢嬰e" u穎"f映pi"vtqpi"8k隠w"vt鵜"ejq"eƒe"d羽pj"pj¤p"d鵜"t逸p"n映e"e逸p0"Jk羽p"pc{."jw{院v"vjcpj" mjƒpi"p丑e"t逸p"n映e"fq"Xk羽p"X逸e"zkp"Pjc"Vtcpi"u違p"zw医v"8«"8逢嬰e"n逢w"j pj"vt‒p"vj鵜" vt逢運pi0"Vtqpi"pijk‒p"e泳w"p {."ej¿pi"v»k"zƒe"8鵜pj"jk羽w"sw違"vtwpi"j”c"p丑e"t逸p"n映e"
59
vq p"rj亥p"v衣k"ikƒ"vt鵜"5OJF"e栄c"mjƒpi"jw{院v"vjcpj"vj逢挨pi"o衣k0"Jw{院v"vjcpj"8逢嬰e" rjc"nq«pi"d壱e"jck"314-1/4-1/8-1/16-1/32 x "栄"épi"x噂k"5OJF"p丑e"荏"590C trong 30
rj¿v0"Vk‒o"2.3"on"x q"x́pi"n逢pi"ejw瓜v"pj逸v"vt逸piswc"8逢運pi"vk‒o"f逢噂k"fc0"Uau 2
ik運."ik違k"rj磯w"ejw瓜v"x "swcp"uƒv"8逢運pi"m pj"x́pi"zw医v"jw{院v0"O厩k"j o"n逢嬰pi"vk‒o" n員r"n衣k"vt‒p"6 ejw瓜v0"O磯w"8嘘k"ej泳pi"mj»pi"e„"jw{院v"vjcpj0 DＶpi"4.9: Vj穎"pijk羽o"vtwpi"j”c"5OJF"p丑e"e栄c"jw{院v"vjcpj"mjƒpi"p丑e Nk隠w (i1ejw瓜v) Jw{院v"vjcpj" pha loãng N逢嬰pi"jw{院v" thanh132"ejw瓜v (µg/µl) U嘘" ejw瓜v (con ) N逢嬰pi"8瓜e" v嘘132"ejw瓜v (µg) V雨"n羽" u嘘pi 3MHD =6µg 1/2 23,5 6 60 6/6 1/4 11,75 6 60 6/6 1/8 5,875 6 60 6/6 1/16 2,937 6 60 6/6 1/32 1,468 6 60 6/6 KHT 1/32 KHT 1/16 KHT 1/8 KHT ¼ KHT 1/2 3MHD Hình 4.9: Mjƒpi"jw{院v"vjcpj"vtwpi"j”c"p丑e"t逸p"n映e"vq p"rj亥p M院v"sw違"ejq"vj医{."v衣k"p欝pi"8瓜"jw{院v"vjcpj"rjc"nq«pi"31:"*2.7:97"i1n+"8«"ejq" rjfir"ej泳pi"okpj"mj違"p<pi"vtwpi"j”c"i亥p"pj逢"jq p"vq p"x "v衣k"p欝pi"8瓜"jw{院v"thanh pha loãng 1/4 (1,175 µg/µL+"ejq"vj医{"mjƒpi"jw{院v"vjcpj"jq p"vq p"vtwpi"j”c"8逢嬰e" p丑e"t逸p"n映e"vq p"rj亥p0
Vjgq"vƒe"ik違"Pigv"Jqpi"Vcp"x "e瓜pi"u詠"8«"vk院p"j pj"vtwpi"j”c"8瓜e"v pj"e栄c"p丑e"
T. albolabris và kjƒpi"jw{院v"vjcpj"mjƒpi"p丑e"x噂k"p欝pi"8瓜"vtwpi"j”c"pj逢"ucw<"8嘘k"
v噂k"mjƒpi"jw{院v"vjcpj"mjƒpi"p丑e"Trimeresurus 8c"ikƒ"PKRO"*National Institute of Preventive Medicine, Taiwan): 2,0 µg/µL (µg trung hòa µl mjƒpi" jw{院v" vjcpj+."
60
mjƒpi"jw{院v"vjcpj"8挨p"ikƒ"IRX"*Vjck"Tgf"Etquu"Uqekgv{."Vjckncpf+<"3.;"µg/µL.
Mjƒpi"jw{院v"vjcpj"PKRO"8逢嬰e"rjc"nq«pi"*3<:+"*2.47"µg/µL+."n逢嬰pi"p丑e"u穎"f映pi"
T.albolabris *5.7"i+""8栄"8吋"i¤{"v鰻p"vj逢挨pi"8逢運pi"m pj"33oo"荏"ejw瓜v [40].
Vtqpi"pijk‒p"eとw"p {."ej¿pi"v»k"vjぬ"pijkうo"vtwpi"j”c"vＴk"pげpi"8じ"jw{ｘv"vjcpj"
pha loãng 1/8 (0,5875 µg/µL+"8«"ejq"rjfir"ejとpi"okpj"mjＶ"p<pi"vtwpi"j”c"iＺp"pj⇔" jq p"vq p"x "vＴk"pげpi"8じ"jw{ｘv"vjcpj"rjc"nq«pi"316"*3.397"µg/µL+"ejq"vjＸ{"mjƒpi" jw{ｘv"vjcpj"jq p"vq p"vtwpi"j”c"8⇔ぢe"pがe"tｈp"nつe"vq p"rjＺp"5OJF"*8.2"i+0"Pj⇔" xｆ{." pげpi" 8じ" jw{ｘv" vjcpj" *205;4"µg/µL+" 8«" vtwpi" j”c" jq p" vq p" n⇔ぢpi" pがe" V0cndqncdtku"*4.2"i+"nkｚw"vぐk"vjkあw"i¤{"zwＸv"jw{ｘv"8⇔ぜpi"m pj"32oo0"Uq"uƒpj"xずk" vj "pijkうo"vt‒p."pげpi"8じ"mjƒpi"jw{ｘv"vjcpj"8あ"vtwpi"j”c"8⇔ぜpi"m pj"33"oo"eてc" vƒe"ikＶ"Pigv"Jqpi"Vcp"x "eじpi"uば""n "2.47"µg/µL."uq"xずk"vj "pijkうo"ej¿pi tôi là 0,392 µg/µL [40] . Swc"8„."ejとpi"vぎ"mjƒpi"jw{ｘv"vjcpj"mjƒpi"pがe"eてc"Xkうp"Xｈe"zkp Pjc"Vtcpi"e„"vƒe"fつpi"vtwpi"j”c"vj pj"rjＺp"i¤{"zwＸv"jw{ｘv"8⇔ぢe"vjぬ"pijkうo"vt‒p" ejwじv0
61
EJ姶愛PI"7< M蔭V"NW一P"ÎMK蔭P"PIJ卯
5.1. M院v"nw壱p
Swc"pijk‒p"e泳w"vt‒p."ej¿pi"v»k"zƒe"8鵜pj"vj pj"rj亥p"i¤{"8瓜e"x "jq衣v"v pj"ukpj" j丑e"e栄c"p丑e"t逸p"n映epj逢"ucw<
+ A瓜e"n詠e"e栄c"p丑e"t逸p"n映e"8w»k"8臼"*Trimeresurus albolabris+"荏"Xk羽v"Pco"n "2.686" i1ị"ejk院o"8瓜e"n詠e"mjƒ"ecq"uq"x噂k"t逸p"n映e"épi"j丑0
+ Nk隠w"i¤{"zw医v"jw{院v"v嘘k"vjk吋w"8逢運pi"m pj"32oo"x́pi"f逢噂k"fc"ejw瓜v"e栄c"p丑e" t逸p"n映e"8w»k"8臼<"OJF?2µg.
+ U逸e"m#"ƒk"n詠e"swce瓜v"Dgp¦cokfkpg"Ugrjctqug®8D"vjw"8逢嬰ej o"n逢嬰pi"rtqvgkp" dƒo"vt‒p"e瓜v"dgp¦cokfkpg<"3.;:"oi"*ejk院o"4.;8'+0
+ U逸e"m#"vtcq"8鰻k"kqp"FGCG"Ugrjctqug® CL-8D"vjw"8逢嬰e"j o"n逢嬰pi"rtqvgkp"
bám vt‒p" e瓜v" FGCG: 12,855 mg *ejk院o" 52,39%). A瓜e" n詠e" rj亥p" dƒo<"
LD50=4,791 i1ejw瓜v"42i"*2.45;"i1i+0
+ Vt丑pi"n逢嬰pi"rj¤p"v穎"e栄c"rtqvgkp"e„"8瓜e"n詠e<"46"mFc
+ A吋"pi<p"pi瑛c"vtk羽w"ej泳pi"zw医v"jw{院v"mjk"pjk宇o"8瓜e"p丑e"t逸p"n映ẹ"mjƒpi"jw{院v" vjcpj" mjƒpi" p丑e" e„" mj違" p<pi" vtwpi" j”c" vƒe" 8瓜pi" zw医v" jw{院v" 荏" 5OJF" n "
1,175µg.
5.2. Mk院p"pij鵜
V瑛"pj英pi"m院v"nw壱p"vt‒p"ej¿pi"v»k"zkp"8逢c"tc"o瓜v"u嘘"8隠"zw医v"pj逢"ucw<
- Zƒe" 8鵜pj" 8瓜e" n詠e" rj亥p" dƒo" ucw" u逸e" m#" ƒk" n詠e" swc" e瓜v" Dgp¦cokfkpg"
Sepharose 6B.
- E亥p"e„"pijk‒p"e泳w"vj‒o"rj逢挨pi"rjƒr"ej嘘pi"n衣k"eƒe"dk院p"ej泳pi"d羽pj"n#"e栄c" pjk宇o"8瓜e"p丑e"t逸p"n映e"8w»k"8臼"fq"ugtkpg"rtqvgcug"gây nên.
- E亥p"u穎"f映pi"mjƒpi"jw{院v"vjcpj"mjƒpi"p丑e"t逸p"n映e"mjk"vck"p衣p"fq"t逸p"e逸p"m院v" j嬰r"x噂k"dk羽p"rjƒr"ej嘘pi"i¤{"nqfiv"pic{"v瑛"8亥w"e pi"u噂o"e pi"v嘘v0
- Vtqpi"vj pj"rj亥p"p丑e"t逸p"n映ẹ"piq k"gp¦{o"ugtkpg"rtqvgcug"e„"vƒe"f映pi"i¤{"
loét còn có enzym metallo protease e pi"e„"mj違"p<pi"i¤{"8瓜e0"P院w"8隠"v k" vk院r"v映ẹ"vk院p"j pj"vkpj"ej院"ogvcnnq"rtqvgcug"x "uq"uƒpj"mj違"p<pi"i¤{"8瓜e" x噂k"ugtkpg"rtqvgcug0
62
VÉK"NK烏W"VJCO"MJ謂Q VÉK"NK烏W"VK蔭PI"XK烏V
[1] N‒" A泳e"V¤ọ"Piw{宇p"Vjk"M‒." N‒"X<p" Dfi." N‒" X<p"Jk羽r"*4225+. "Pijk‒p"eとw"vjぬ"
pijkうo"vt‒p"pi⇔ぜk"jw{ｘv"vjcpj"mjƒpi"pがe"tｈp"jご"8Ｘv"*Pclc"mcqwvjkc+."jw{ｘv"vjcpj" mjƒpi"pがe"tｈp"nつe"vtg"*Vtkogtguwtwu"cndqncdtkư", V衣r"ej "["j丑e"f詠"rj”pị"ZKKK."850 [2] Piw{宇p"X<p"Uƒpị"J欝"Vj鵜"Vjw"E¿ẹ"Piw{宇p"Sw違pi"Vt逢運pi0"Fcpj"nつe"d”"uƒv"ｘch nhái
Xkうv"Pco0"ZPD"PP"J "P瓜k."4227."36:0
[3] X "X<p"Ejk."Piw{宇p"A泳e"Okpj0"Tｈp"n o"vjwぐe"x "vjwぐe"vtお"tｈp"eｈp. NXB KH - KT, J "P瓜k<"4222."480
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66
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68
RJ影"N影E"K
E医w"vt¿e"rj¤p"v穎"VNEM-Trypsin
E医w"vt¿e"rj¤p"v穎"e栄c"VNEM"x "rj泳e"j嬰r"e栄c"p„"x噂k"Vt{rukp."8逢嬰e"dk院v"8院p"pj逢" ej医v"e¤p"d茨pi"kqp"j„c"*cekf1"dc¦挨+"pj„o"j{ftqz{n"e栄c"jgokmgvcn"x "x鵜"vt "qz{"j„c"kqp"
69
RJ影"N影E"KK
1. Oƒw<"eƒe"vj pj"rj亥p"f鵜ej"vj吋
O丑k"v院"d q"vtqpi"e挨"vj吋"8隠w"vj逢運pi"zw{‒p"e亥p"8逢嬰e"pw»k"f逢叡pi"x "d違q"x羽. Máu 8欝pi" vj運k"8違o"d違q"e違"jck"pjw"e亥w"8„"ejq"o丑k"v院"d qvtqpi"e挨"vj吋.
Pw»k"f逢叡pi"eƒe"v院"d q"e„"pij c"n "ewpi"e医r"eƒe"ej医v"ejq"v院"d q"vk‒w"vj映"*Q2, glucose, axit-cokp."nkrkfụ"mjqƒpi"ej医v."eƒe"ej医v"8k羽p"rj¤p"È+"x "vjw"f丑p"eƒe"ej医v"v院" d q"d k"vk院v"*EQ2."wt‒."ow嘘k"vj瑛cÈ+0"U詠"ejw{吋p"j„c"pj壱r"p<pi"n逢嬰pi"e亥p"ejq"v院"d q" jq衣v"8瓜pi0"Épi"x噂k"eƒe"ej医v"ejw{吋p"j„c."oƒw"e”p"rj¤p"rj嘘k"pjk隠w"ukpj"rj育o"ocpi" ej泳e"p<pi"ukpj"n#"*jqtoqpgụ"e{vqmkpgụ"cndwokp."o瓜v"u嘘"{院w"v嘘"e亥o"oƒw."vtqpi"8„" hkdtkpqigp"n "{院w"v嘘"K+0"V医v"e違"eƒe"ej医v"8„"8隠w"荏"f衣pi"vcp"vtqpi"rj亥p"n臼pi"e栄c"oƒw"i丑k" n "jw{院v"v逢挨pi"*rncuoc+0"雲"pi逢運k"vt逢荏pi"vj pj."v鰻pi"mj嘘k"jw{院v"v逢挨pi"ejk院o"mjq違pi"5" n v."n "77'"e栄c"v鰻pi"mj嘘k"oƒw"*7.6"-7.7"n v+0"P逢噂e"n "vj pj"rj亥p"n噂p"pj医v"e栄c"jw{院v"v逢挨pi" *;4'+0"N逢嬰pi"p逢噂e"p {"e pi"pj逢"eƒe"vj pj"rj亥p"mjƒe"nk‒p"v映e"j茨pi"pi {"8逢嬰e"vjc{" o噂k."swc"8逢運pi"vk‒w"j„c."x "8逢運pi"j»"j医r"*p逢噂ew嘘pi"x "vj詠e"rj育ơ0"Eƒe"rj¤p"v穎"n噂p" pj逢"eƒe"rtqvgkp"ej栄"{院w"8逢嬰e"icp"vƒk"ukpj0"Jw{院v"v逢挨pi"e”p"ejw{吋p"v違k"eƒe"v院"d q"oƒw." u違p"ukpj"v瑛"v栄{"z逢挨pi"x "nƒej0"Uq"x噂k"mj嘘k"o厩k"v院"d q"mjƒe"pjcw"ejk院o"vtqpi"oƒw."j欝pi" e亥w"ejk院o"mj嘘k"n逢嬰pi"n噂p"pj医v0"Ej¿pi"vc"e亥p"j·pj"fwpi"t茨pi"o厩k"ik¤{."v栄{"z逢挨pi"8逢c" x q"oƒw"n逢w"j pj"4"Î5"vtk羽w"j欝pi"e亥w."ucw"vj運k"ikcp"mjq違pi"342"pi {"o厩k"j欝pi"e亥w"u胤" 8逢嬰e"vjc{"vj院="vtqpi"o瓜v"oo3 oƒw"d·pj"vj逢運pi"e„"6"Î 8"vtk羽w"j欝pi"e亥w0"J欝pi"e亥w" vt逢荏pi"vj pj"*tgf"dnqqf"egnn."TDE+"8院p"v瑛"jw{院v"d q"*gt{vjtqe{vg."ijfir"v瑛 erythros là
8臼."m{vqu"pij c"n "嘘pi"t厩pi"jq員e"e{vg"n "v院"d q+."pj逢pi"pj¤p"e栄c"jw{院v"d q"8«"o医v"8k" 8吋"pj逢運pi"ej厩"v嘘k"8c"ejq"jw{院v"u逸v"v嘘"*jgoqinqdkp."Jd+."n "rtqvgkp"ejw{吋p"v違k"Q2 và CO2."x "8„"e pi"n "ej泳e"p<pi"ej栄"{院w"e栄c"j欝pi"e亥w0"Vtqpi"oƒw"e”p"o瓜v"v院"d q"mj»pi" pj¤p"mjƒe"n "vk吋w"e亥w"*rncvgngv+."n "pj英pi"o違pj"pj臼"e栄c"vk隠p"vk吋w"e亥w"n "o瓜v"v院"d q"mj鰻pi" n欝"*ogicmct{qe{vg+0"Vk吋w"e亥w"e”p"8逢嬰e"i丑k"n "jw{院v"mj嘘k"d q"*vjtqodqe{vg v瑛"J{-n衣r" "e„"pij c"n "mj嘘k"x "v瑛" k "n "v院"d q+."fq"e„"ej泳e"p<pi"swcp"vt丑pi"vtqpi"u詠" e亥o"oƒw."épi"jq衣v"8瓜pi"x噂k"vjtqodkp"8吋"v衣q"jw{院v"mj嘘k"*vjtqodwư0"Jw{院v"mj嘘k"n o" pij胤p"o衣ej."8員e"dk羽v"i¤{"d羽pj"n#"vko"o衣ej0 A吋"d違q"x羽"eƒe"v院"d q"vtqpi"e挨"vj吋."oƒw"ewpi"e医r"荏"p欝pi"8瓜"e亥p"vjk院v"eƒe"ej医v"ej嘘pi" pjk宇o"f鵜ej"vj吋"*j羽"vj嘘pi"d鰻"vj吋."eƒe"rtqvgkp"rj違p"泳pi"e医r."mjƒpi"vj吋È+"x "8逢c"eƒe"
70
d衣ej"e亥w"n "eƒe"v院"d q"ok宇p"pjk宇o"nk‒p"v映e"vw亥p"jq p"ikƒo"uƒv"8吋"ecp"vjk羽r"m鵜r"vj運k0" Ej医v"dgvc-n{ukp"fq"vk吋w"e亥w"vk院v"tc"e pi"vjco"ikc"pi<p"mjw育p"z¤o"pj壱r0
Ej泳e"p<pi"d違q"x羽"mj»pi"ej雨"ik噂k"j衣p"荏"cáe"rj違p"泳pi"ok宇p"pjk宇o"o "e”p"e„ u詠"rj嘘k"j嬰r"x噂k"eƒe"ej泳e"p<pi"ukpj"n#"mjƒe"pj逢"e亥o"oƒw."vƒk"v衣q"v鰻p"vj逢挨pị"jq衣v"8瓜pi" 8k隠w"vk院v"ik英"i·p"e¤p"d茨pi"ukpj"j丑e0
Oƒw"vw亥p"jq p"mj逸r"e挨"vj吋."nk‒p"j羽"x噂k"o丑k"v院"d q"x "ejk院o"vq p"d瓜"mj»pi"ikcp" ik英c"eƒe"o»"x "v院"d q."fq"8„"oƒw"8逢嬰e"swcp"pk羽o"pj逢"n "o瓜v"o»"nk‒p"m院v0"E亥o"oƒw" *jgoquvcukư"x "8»pi"oƒw"*eqciwncvkqp+"n "pj英pi"e挨"ej院"j茨pi"8鵜pj"e栄c"e挨"vj吋"pj茨o" fw{"vt·"u詠"vq p"x姻p"e栄c"vj pj"o衣ej."f詠"rj”pi"ej違{"oƒw"x "e亥o"oƒw0"U詠"e亥o"oƒw"x " 8»pi" oƒw" 8逢嬰e" 8違o" d違q" pj運" 荏" jq衣v" 8瓜pi" e栄c" e挨" ej院" v衣q" x " n o" vcp" jw{院v" mj嘘k" *vjtqodqigpgukụ"vjtqodqn{ukư0"Fq"jw{院v"mj嘘k"e違p"vt荏"u詠"vw亥p"jq p"p‒p"o瓜v"e挨"ej院"u印p" e„"8吋"vw"ej雨pj"mj»pi"8吋"jw{院v"mj嘘k"v欝p"v衣k"vtqpi"jw{院v"o衣ej."8„"n "u詠"vcp"jw{院v"mj嘘k" *vjtqodqn{ukư0"雲"o瓜v"u嘘"vt逢運pi"j嬰r"d羽pj"n#."f́"mj»pi"e„"ej医p"vj逢挨pi"o荏"o»"swcpj" jw{院v"o衣ej"jw{院v"mj嘘k"n衣k"n "piw{‒p"pj¤p"n o"pij胤p"jw{院v"o衣ej."o瓜v"piw{"e挨"e„"vj吋" f磯p"8院p"v穎"xqpị"p院w"mj»pi"8逢嬰e"e泳w"ej英c"m鵜r0"P逸o"x英pi"x隠"e挨"ej院"8»pi"e亥o"oƒw" d·pj"vj逢運pi"u胤"jk吋w"t "e挨"ej院"vƒe"8瓜pi"e栄c"eƒe"vj pj"rj亥p"p丑e t逸p"n‒p"j羽"vj嘘pi"8»pi"
máụ
Mjk"x pj"jw{院v"o衣ej"d鵜"v鰻p"vj逢挨pi"o荏."oƒw"x "x pj"o衣ej"mj荏k"8瓜pi"e挨"ej院"8»pi"oƒw." v衣q"mj嘘k"jw{院v."n o"pi瑛pi"vjqƒv"oƒw"x "pi<p"ej員p"pjk宇o"mjw育p0"Mj嘘k"jw{院v"8„"ejq" j·pj"fwpi"f宇"j挨p"j·pj"違pj"e栄c"o瓜v"o»"nk‒p"m院v"vtqpi"8„"hkdtkpqigp"vcp"vtqpi"jw{院v" v逢挨pi"vt荏"vj pj"n逢噂k"vt́pi"j嬰r"rj¤p"v穎"hkdtkp"vjw"i丑p"vq p"d瓜"vk吋w"e亥w"x "v院"d q"oƒw" vj pj"ṕk."nq衣k"vt瑛"rj亥p"n臼pi"jw{院v"vjcpj"*jw{院v"v逢挨pi"8«"nq衣k"vt瑛"hkdtkpqigp+0"Mjk"oƒw" tc"mj臼k"jw{院v"o衣ej."ejq"ej違{"x q"嘘pi"pijk羽ọ"oƒw"e pi"v詠"8»pi"n衣k"vj pj"mj嘘k"jw{院v0
2. E亥o"oƒw"x "jw{院v"mj嘘k
Jw{院v"o衣ej"ejkc"vj pj"d嘘p"n噂r< rj亥p"n”pi"嘘pi"*nwogp+."rj栄"swcpj"x噂k"o瓜v"p隠p"v院"d q" p瓜k"o»"*gpfqvjgnkcn"egnn+"x "rj亥p"x pj"i欝o"dc"n噂r<"x pj"ƒq"vtqpi"*vwpkec"kpvkoc+."x pj" ƒq"ik英c"*vwpkec"ogfkc+"x "x pj"x臼"piq k"*vwpkec"cfxgpvkvkc+0
71
Okpj"j丑c"v鰻"ej泳e"e栄c"jw{院v"o衣ej"[6]
E挨"ej院"e亥o"oƒw [12]
72
X pj"jw{院v"o衣ej"e栄c"j羽"vj嘘pi"vw亥p"jq p"x "eƒe"vj pj"rj亥p"e栄c"oƒw"8逢嬰e"vjk院v" n壱r"8吋"v衣q"mj違"p<pi"e亥o" oƒw"荏"pi逢運k"d·pj"vj逢運pi0"J羽"vj嘘pi"eƒe" {院w"v嘘"e亥o" oƒw"
(thrombogenic 1"jgoquvcvke"hcevqtư"i欝o"o瓜v"u嘘"vj pj"rj亥p"e„"u印p"vtqpi"oƒw."o瓜v"u嘘" fq"eƒe"v院"d q"vk院v"tc."o瓜v"u嘘"n "vj pj"rj亥p"e栄c"o»"x "v院"d q"x pj"jw{院v"o衣ej0"A吋"i丑k" eƒe"{院w"v嘘"p {"o瓜v"eqp"u嘘"8«"8逢嬰e"i逸p."vjgq"vj泳"v詠"e栄c"u詠"rjƒv"jk羽p"các rtqvgkp"p {0"Ac" u嘘"eƒe"{院w"v嘘"8„"n "pj英pi"vk隠p"ugtkpg"protease *¦{oqigp"1"rtqrtqvgkpcug+"jq員e"n "8欝pi" {院w"v嘘"*eqhcevqt."jq衣v"8瓜pi"m院v"j嬰r"x噂k"gp¦{og). Hình 2.13 minh j丑c"v鰻"ej泳e"e栄c"jw{院v" o衣ej"vj pj"d嘘p"n噂r."rj亥p"n”pi"嘘pi"*nwogp+."rj栄"swcpj"x噂k"o瓜v"p隠p"v院"d q"p瓜k"o»" *gpfqvjgnkcn"egnn+"x "rj亥p"x pj"i欝o"dc"n噂r<"x pj"ƒq"vtqpi"*vwpkec"kpvkoc+."x pj"ƒq"ik英c" *vwpkec"ogfkc+"x "x pj"x臼"piq k"*vwpkec"cfxgpvkvkc+0"X pj"o衣ej"rj c"vt‒p"jình minh
j丑c"x pj"o衣ej lành, bình tj逢運pi="x pj"o衣ej"rj c"f逢噂k"e栄c"j·pj"okpj"j丑c"o瓜v"x pj" o衣ej" Òz挨" x英cÓ" *cvjgtqoc=" cvjgtqvjtqodquku=" cvjgtquengtquku" mjk" e„" pj英pi" n噂r" o叡" pi逢pi"8丑pi+0"X pj"o衣ej"n pj"o医v"u泳e"d隠p"f飲q"d·pj"vj逢運pị"x·"8«"vj pj"n壱r"o瓜v"n噂r" o»"nk‒p"m院v"vtqpi"8„"pjk隠w"eqnncigp."zgp"m胤"d衣ej"e亥w"8挨p"pj¤p."8衣k"vj詠e"d q"pi壱o"8亥{" ej医v"dfiq"*hqco"egnnự"v院"d q"e挨"vt挨p"*uoqqvj"owueng"egnn+."piw{‒p"v院"d q"u嬰k"*hkdtqdncuv+." x "eƒe"f衣pi"qz{"j„c"e栄c"nkrqrtqvgkp"vt丑pi"n逢嬰pi"vj医r"*nqy"fgpukv{"nkrqrtqvgkp."QzNFN+0" Jck"v院"d q"vj逢運pi"rj„ng ra superoxide (O2‚/) *u違p"rj育o"ejw{吋pj„c"v瑛"vk"vj吋"x "PCFRJ"
oxidase, mjk"d鵜"vjk院w"j映v"Q2x "o瓜v"u嘘"vt逢運pi"j嬰r"mjƒe"(reactive oxygen species, ROS)
n "8衣k"vj詠e"d q"x "v院"d q"p瓜k"o»0"O2‚/ có n詠e"rj違p"泳pi"j„c"j丑e"o衣pj."oc{"n "vj運k"ikcp" u嘘pi"e栄c"p„"t医v"pi逸p0"Pj逢pi"p院w"mj育w"rj亥p"<p"ik w"ej医v"dfiq"8瓜pi"x壱v."p欝pi"8瓜"NFN" oƒw"ecq."u詠"u違p"ukpj"QzNFN"n "8k隠w"v医v" {院w0"QzNFN"jq衣v"j„c"d衣ej"e亥w"8挨p"pj¤p" *oqpqe{vg+"vj pj"8衣k"vj詠e"d q"8吋"f丑p"u衣ej"eƒe"8k吋o"o叡"8丑pị"vjco"ikc"vƒk"v衣q"p隠p"v院"
b q"p瓜k"o»0"Eƒe"x pj"8瓜pi"o衣ej"Òz挨"x英cÓ"n "o瓜v"piw{"e挨"v衣q"jw{院v"mj嘘k"*vjtqodwự" e„"vj吋"f磯p"8院p"pij胤p"jw{院v"o衣ej."i¤{"j壱w"sw違"vt亥o"vt丑pi0"Fq"z挨"x英c"n o"n瓜"tc"{院w"v嘘"
mô TF mj荏k"8瓜pi"e亥o"oƒw [39].
Eƒe"v院"d q"p瓜k"o»"swcpj"x pj"jw{院v"o衣ej"p院w"d鵜"v鰻p"vj逢挨pi"u胤"n o"n瓜"tc"o瓜v"rtqvgkp" vq p"vcp"vtqpi"o pi"v院"d q"*kpvgitcn"ogodtcpg"rtqvgkp."pij c"n "rtqvgkp"vcp"vtqpi"o叡" vjc{"ejq"vcp"vtqpi"p逢噂e"pj逢"eƒe"rtqvgkp"vj»pi"vj逢運pi+="rtqvgkp"o pi"p {"8逢嬰e"dk吋w" jk羽p"e”p"pjk隠w"j挨p"vt‒p"o pi"e栄c"piw{‒p"v院 d q"u嬰k."v院"d q"e挨"vt挨p."8隠w"e„"o員v"vtqpi" x́pi"d鵜"z挨"x英c0"Rtqvgkp"o pip„k"8¤{"n "{院w"v嘘"o»"*VH."vkuuwg"hcevqt."hình 2.13+0"[院w" v嘘"VH"n "vw{羽v"8嘘k"e亥p"vjk院v"ejq"u詠"e亥o"oƒw"d·pj"vj逢運pi. Xck"vt”"ukpj"j丑e"swcp"vt丑pi"
73
e栄c"{院w"v嘘"VH"ejq"jk吋w"e„"o嘘k"nk‒p"swcp"o壱v"vjk院v"ik英c"oƒw1jw{院v"x "n逢噂k"嘘pi"o衣ej" 8違o"pjk羽o"vw亥p"jq p."j羽"vj嘘pi"jw{院v"o衣ej"n "o瓜v."oƒw"jq衣v"8瓜pi"eƒej"nk‒p"m院v"vjk院v" n壱r"x噂k"嘘pi"o衣ej0"
Trên hình 2.14, ej¿pi"vc"mj»pi"p‒p"n逢w"v¤o"8院p"x鵜"vt "e栄c"jck"v瑛"ÒkpvtkpukeÓ"*p瓜k"v衣k+"x " Ògzvtkpuke"rcvjyc{Ó"*mj荏k"8瓜pi"ik¤{"ejw{隠p"rj違p"泳pi"8»pi"oƒw"fq"o瓜v"piw{‒p"pj¤p" v瑛"piq k+."ej¿pi"vc"e„"vj吋"8違q"pi逢嬰e"x鵜"vt "e栄c"jck"v瑛"8„"8吋"vj医{"t "j挨p"jq衣v"8瓜pi"e亥o" oƒw"p瓜k"v衣k"mj荏k"8瓜pi"x噂k"u詠"m院v"j嬰r"e栄c"jck"{院w"v嘘"XKK"x "VH."jck"rtqvgkp"e„"ƒk"n詠e"x噂k" pjcw"*{院w"v嘘"XKK"荏"f衣pi"XKKc"x磯p"ik英"ƒk"n詠e"x噂k"VH+0"[院w"v嘘"XKK"e„"u印p"vtqpi"oƒw"vw亥p" jq p."p院w"i員r"rtqvgkp"o pi"VH"d医v"vj逢運pi"n瓜"tc."rj泳e"j嬰r"VH<XKK"8逢嬰e"v衣q"vj pj0"Mjk" o pi"v院"d q"x "x pj"o衣ej"dk"v鰻p"vj逢挨pi"p瓜k"v衣k."VH<XKK"zw医v"jk羽p."pj逢pi"ej逢c"8k"x q" jq衣v"8瓜pi"8吋"v衣q"jw{院v"mj嘘k"v衣k"ej厩"*VH"i逸p"nk隠p"x噂k"o pi"v院"d q"p‒p"8泳pi"n衣k"v衣k"ej厩+0" [院w"v嘘"XKK"e亥p"ejw{吋p"swc"f衣pi"jq衣v"j„c"XKKc"8吋"jq衣v"j„c"{院w"v嘘"Z0"VH"n "o瓜v"8欝pi" {院w"v嘘"8吋"épi"jq衣v"8瓜pi"x噂k"XKKc."eqnncigp"x "vk吋w"e亥w"e pi"épi"vjcoikc0"E pi"pj逢" {院w"v嘘"XKK."jck"{院w"v嘘"Z"x "KZ"8隠w"e„"u印p"vtqpi"jw{院v"v逢挨pị"pj逢pi"ej逢c"e„"jq衣v"v pj" rtqvgcug"ejq"8院p"mjkÈ8逢嬰e"ej pj"{院w"v嘘"XKKc"jq衣v"j„c."v泳e"o瓜v"rj違p"泳pi"j欝k"vƒe"f逢挨pi"
(positive feedback reaction).
U詠"8k隠w"vk院v"e挨"ej院"v衣q"jw{院v"mj嘘k [45]
Vjtqodkp"*KKc+"8嘘e"vj¿e"1"jq衣v"j„c"p<o"rj違p"泳pi"vtqpi"ik¤{"ejw{隠p0 VHRK"d医vjq衣v"u詠" mj荏k"8瓜pi"8逢運pi"e亥o"oƒw"p瓜k"v衣k0"CVKKK."jgrctkp"x "Ec--"¿e"ej院"jck"ugtkpg"rtqvgcug" Zc" x " KKc0" U詠" ejw{吋p" f衣pi" e医w" vt¿e" e栄c" CVKKK" vtqpi" nk‒p" m院v" x噂k" jgrctkp" x " ugtkpg" rtqvgcug"*cnnquvgtke"kpvgtcevkqpư"8k隠w"ej雨pj"jq衣v"8瓜pi"e栄c"CVKKK0
74
[院w"v嘘"XKKc"e”pjq衣v"j„c"{院w"v嘘"KZ"vj pj"KZc0"Jck"{院w"v嘘"KZc"x "XKKc"8隠w"jq衣v"j„c"{院w" v嘘"Z."pj逢pi"jq衣v"8瓜pi"e栄c"KZc"e”p"e亥p"u詠"vjco"ikc"e栄c"eƒe"8欝pi"{院w"v嘘"mjƒe"n "XKKKc." Ec--"x "rjqurjqnkrkf"ocpi"eƒe"pj„o"v pj"8k羽p"¤o"e„"pjk隠w"vt‒p"o pi"e栄c"vk吋w"e亥w0 Ak隠w"o "hình 2.15 mw嘘p"p‒w"n‒p"n "u詠"v<pi"v嘘e"jq衣v"8瓜pi"e亥o"oƒw"swc"j羽"vj嘘pi"eƒe" rj違p"泳pi"j欝k"vƒe"8嘘e"vj¿e0Ew嘘k"épi"n逢噂k"hkdtkp"8逢嬰e"鰻p"8鵜pj"x噂k"pj英pi"nk‒p"m院v"picpi" fq"{院w"v嘘"ZKKKc"m院v"p嘘k0"Vk吋w"e亥w"jq衣v"j„c"vk院v"tc"{院w"v嘘"ZKKK"x "vjtqodkp"jq衣v"j„c"vj pj" ZKKKc0"[院w"v嘘"ZKKKc"n "o瓜v"vtcpuinwvcokpcug0"Jk羽w"sw違"8»pi"oƒw"t医v"ecq"pj運"荏"e挨"ej院" e亥o"oƒw"e„"mj違"p<pi"mjw院ej"8衣k"n噂p"swc"jq衣v"8瓜pi"e栄c"eƒe"gp¦{og0 Vtqpi"u詠"8k隠w"vk院v"e栄c"j羽"e亥o"oƒw."e„"o瓜v"u嘘"e挨"ej院"mjƒe"pjcw<"rj違p"泳pi"j欝k"vƒe"¤ọ" ik違o"v嘘e"d茨pi"eƒej"泳e"ej院"jq員e"d医v"jq衣v"eƒe"gp¦{og"jq衣v"8瓜pi"e亥o"oƒw0"Jck"rtqvgcug" swcp"vt丑pi"pic{"vt逢噂e"ikck"8q衣p"vj栄{"rj¤p"hkdtkpqigp"n "{院w"v嘘"Zc"x "KKc."v泳e"vjtqodkp0" Cpvkvjtqodkp"KKK"*CVKKK+"e”p"8逢嬰e"i丑k"n "g-cpvkvjtqodkp"n "f衣pi"e„"8亥{"8栄"6"pj„o" in{equ{n"vt‒p"rj¤p"v穎"rtqvgkp"x "n "f衣pi"ej pj"e„"o員v"vtqpi"jw{院v"v逢挨pi0"CVKKK"泳e"ej院" jck"ugtkpg"rtqvgcug"Zc"x "KKc"x瑛c"p‒w."d茨pi"eƒej"v衣q"nk‒p"m院v"x q"pic{"vtwpi"v¤o"z¿e"vƒe" ugtkpg."x "u詠"nk‒p"m院v"ik英c"gp¦{og"Zc1KKc"x "CVKKK"vt荏"p‒p"o衣pj"j挨p"mjk"e„"o員v"jgrctkp" *o瓜v"in{equcokpqin{ecp"uwnhcvg"p瓜k"ukph). Ik¤{"ejw{隠p"eƒe"rj違p"泳pi"e„"j欝k"vƒe"n "j羽"vj嘘pi"eƒe"rj違p"泳pi"vtqpi"8„"u違p"rj育o"e栄c" o瓜v"vtqpi"pj英pi"rj違p"泳pi"ew嘘k"vt荏"n衣k"vƒe"8瓜pi"x q"o瓜v"rj違p"泳pi"mj荏k"8亥w."vj逢運pi"n " rj違p"泳pi"8亥w"vk‒p"e栄c"ik¤{"ejw{隠p."v泳e"rj違p"泳pi"8亥w"n羽"vjw瓜e"x q"p欝pi"8瓜"e栄c"u違p" rj育oew嘘k."8吋"v<pi"v嘘e"8瓜"jc{"ik違o"v嘘e"e栄c"rj違p"泳pi"mj荏k""8亥w."ej栄"{院w"8吋"8k隠w"vk院v"u詠" u違p"zw医v"8吋"p欝pi"8瓜"e栄c"u違p"rj育o"ew嘘k"épi"8逢嬰e"rj́"j嬰r"pj医v"ejq"jq衣v 8瓜pi"ukpj"n#0" Zc"jq衣v"j„c"rtqvjtqodkp"vj pj"vjtqodkp0"Vjtqodkp"n衣k"rj違p"j欝k"8嘘e"vj¿e"8院p"p<o"rj違p" 泳pi<"VåVa, VIIIåVIIIa, XIåZKc."rj違p"泳pi (protein C + protein S + TM)å protein
E"jq衣v"j„c"x "rj違p"泳pi"ZKKKåXIIIa, 鰻p"8鵜pj"n逢噂k"hkdtkp"*Hình 2.15+0"V„o"n衣k."mj荏k" 8瓜pi"u詠"e亥o"oƒw"p瓜k"v衣k"ej雨"e亥p"o瓜v"d逢噂e"jq衣v"j„c"XKK"vj pj"XKKc"8吋"8k"x q"jq衣v"j„c" {院w"v嘘"Z"vj pj"Zc0"Pj逢pi"8吋"v衣q"u詠"8»pi"oƒw"mjk"d鵜"ej医p"vj逢挨pi"o荏."e亥p"8院p"d嘘p" rj違p"泳pi"z¿e"vƒe"vt逢噂e"u詠"jq衣v"j„c"{院w"v嘘"Z."p‒p"u詠"8k隠w"vk院v"e pi"rj泳e"v衣r"j挨p."x·" vtqpi"e挨"ej院"8»pi"oƒw"p {"e”p"e„"u詠"8欝pi"jq衣v"8瓜pi"e栄c"pj英pi"rj違p"泳pi"xk‒ọ"ej嘘pi" pjk宇o"d育o"ukpj"pj逢"u胤"8隠"e壱r"pic{"ucw"8¤{0
75
V瑛"u詠"jq衣v"j„c"{院w"v嘘"Z"8院p"u詠"j·pj"vj pj"jw{院v"mj嘘k"*vjtqodqukư"vtqpi"u詠"e亥o"oƒw" *jgoquvcuku1vjtqodqigpgukự"jq員e"8院p"u詠"j·pj"vj pj"mj嘘k"jw{院v"*enqv1eqciwnwơ"e栄c" u詠"8»pi"oƒw"*eqciwncvkqp+n "pj英pi"jq衣v"8瓜pi"ejwpi0
Ak隠w"o "eƒe"j·pj"mj»pi"p„k"n‒p"8逢嬰e"o "ej¿pi"vc"e亥p"j·pj"fwpi"n "e挨"ej院"z¿e"vƒẹ"v衣q" u詠"mjw院ej"8衣k"e栄c"vjƒe"eƒe"rj違p"泳pi0"P院w"mj荏k"8亥w"{院w"v嘘"XKKc"ej雨"n "o瓜v"rj¤p"v穎." pj逢pi"rj¤p"v穎"p {"z¿e"vƒe"rj違p"泳pi"jq衣v"j„c"{院w"v嘘"Z"vj pj"Zc."ej雨"o瓜v"rj¤p"v穎"XKKc" v衣q"p‒p"o瓜v"u嘘"t医v"n噂p."x "f映"8院p"323 rj¤p"v穎"Zc="pij c"n "d逢噂e"vk院r"vjgq"o厩k"rj¤p"v穎" Zc"n衣k"v衣q"p‒p"323rj¤p"v穎"XKKc."x "o瓜v"pi p"rj¤p"v穎"Zc"v衣q"p‒p"323x103 rj¤p"v穎"XKKc." v泳e"326 rj¤p"v穎"XKKc0"U詠"pj¤p"d瓜k"vjgq"nw壱v"u嘘"o "8„"n "o瓜v"8員e"8k吋o"vjk院v"{院w"vtqpi" jq衣v"8瓜pi"e栄c"u詠"e亥o"oƒw8k隠w"j pj"d荏k"eƒe"gp¦{og"8吋"8衣v"jk羽w"sw違0
3. Eƒe"d逢噂e"e挨"d違p"e栄c"swƒ"vt·pj"e亥o"oƒw
J羽"vj嘘pi"eƒe"{ｘw"vぐ"eＺo"oƒw (thrombogenic/ hemostatic factors) i欝o"o瓜v" u嘘"vj pj"rj亥p"e„"u印p"vtqpi"oƒw."o瓜v"u嘘"fq"eƒe"v院"d q"vk院v"tc."o瓜v"u嘘"n "vj pj"rj亥p" e栄c"o»"x "v院"d q"x pj"jw{院v"o衣ej0"
76
RJ影"N影E"KKK
P欝pi"8瓜"eƒe"8羽o"x "ign"vtqpi"UFU-PAGE 12 %
- Dd 40% T Acylamide : 38,93g P."PÓ"Î Methylene Î bisacrylamide : 1,07 g N逢噂e"e医v"x瑛c"8栄 : 100 mL - Dd 9,5% T Acrylamide : 6,3 g P."PÓ"Î Methylene Î bisacrylamide : 3,2 g P逢噂e"e医v"x瑛c"8栄 : 100 mL - Dd 8羽o"rj¤p"ik違k"*3.7"Ợ"rJ":.2 Tris base : 18,15g P逢噂e"e医v"x瑛c"8栄 : 100 mL - Dd 8羽o"v壱r"vtwpi"*2.7Ợ"rJ"8.: Tris base : 3,0 g P逢噂ee医v"x瑛c"8栄 : 100 mL - Dd 8羽o"8k羽p"fk"6Z Tris base : 12,0 g Glycine : 57,6 g P逢噂e"e医v"x瑛c"8栄 : 1000 mL - Dd pjw瓜o"ign
Coomassive Brilliant Blue : 0,625 g
Methanol : 112,5 mL
77 P逢噂e"e医v"x瑛c"8栄 : 250 mL - Dd v育{ Acid acetic (99%) : 75 mL Methanol (99%) : 50 mL P逢噂e"e医v"x瑛c"8栄 : 1000 mL - Dd rjc"o磯w Tris base 0,5M : 2 mL SDS 10% : 2,5 mL Glycerol : 2 mL Xanh Bromophenol : 0,004 % P逢噂e"e医v"x瑛c"8栄 : 10 mL pH : 6,8 ĐT (Dithiothreitol) : 10%
78 A鰻"ign"rj¤p"vƒej"e„"p欝pi"8瓜"Cet{ncokfg"34' H2O 1,440 µL Ff"8羽o"Vtku"3.7Ọ"rJ":.: 1,500 µL Dd 40% Acrylamide 1,800 µL SDS 10% 60 µL Dd Glycerol 1200 µL TEMED 4 µL APS 10% 20 µL A鰻"ign"v壱r"vtwpi"e„"p欝pi"8瓜"Cet{ncokfg"34' H2O 480 µL Ff"8羽o"Vtku"3.7Ọ"rJ"8,8 500 µL Dd 9,5% T Acrylamide 1000 µL SDS 10% 20 µL TEMED 4 µL APS 10% 30 µL
79
EðPI"VTîPJ"EðPI"D渦
1. Piw{宇p"Vj鵜"Piw{羽v"Vjw."X "Rj衣o"J欝pi"Ej¤w."Piw{宇p"V医p"Jk羽r."Piw{宇p"N‒"
Trang (2004+0"Okいp"fおej"vjつ"8じpi"ejぐpi"8じe"vぐ"tｈp"ock"iＺo0"Ejw{‒p"8ｚ"mjƒpi" vjwぐe-okいp"fおej0"M益"[院w"J瓜k"pij鵜"mjqc"j丑e"Xk羽p"Rcuvgwt"Vr0"J欝"Ej "Okpj0" D瓜"["V院."vtcpi"349-131.
2. Piw{宇p"V医p"Jk羽r."Piw{宇p"Vj¿{"J逢挨pị"Piw{宇p"Vj鵜"Piw{羽v"Vjw."Piw{宇p"N‒"
Trang (2014). Zƒe"8おpj"8じe"nばe"eてc"tｈp"nつe"8w»k"8ぎ"x "vƒe"8じpi"i¤{"nqfiv"vt‒p" ejwじv0"V衣r"ej "["j丑e"Vj pj"rj嘘"J欝"Ej "Okpj0 V壱r"3:."U嘘"4."vtcpi"65-49.
80
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