II. TIm hi<i'u 'mh hlidng cua chitosan chie'u x~ lieu 400 kGy len slj tai sinh
CVA CHITOSAN CRIEU X4.
I. Anh hlidng cua vanadium I~n sl/ sinh trlidng cua dly hoa cuc
Chrysanthemum indiclll1lL. in vitro
8()c tlnh vanadium d. dllCic xac dinh r6 lr~n Iua mi, dai mach, b~p cai, Ilia Ill(Oc, v.v, Tuy nhien, cac c6ng boxac djnh dqc linh vanadium dt(qc thtrc
hi~n tmng di6u ki~n kh6ng v6 trung. VI v~y, c6 th<i c6 nhung nhi~u loan gay ra do vi sinhv~t,anh h,ldng de'n tlnh chinh xac cua ke't qua thinghi~m. Do d6, chung toi tie'n hanh thi nghiem xilc dinh d6c tinh vanadium tmng di6u ki~nin
vitroM tranh caenhi~uloan do vi sinh v~tgay ranh~mkhilng dinh them d()c
tinh cua kim loai nay.
Trong thi nghi~m nay, chung t6i cung dilOg cae d6t ngon ctla diy d6i chungM thi nghi~m nh~mbaD dam d() d6ng d6u v6 tu5i sinh Iy cua cay. M6i tnlong cci ban s(1 dung la LS b5 sung vanadium theo cae n6ng d6: 1,0; 2,5; 5,0; 10,0; 30,0; 50,0 va 100 mg!1 (tlnh theo V trong Nt4VO,), dllQc ky hieu la VIII, VII" VII" VII4.VII" VII" VII" va VII,. Sau 30 ngay nu6i cily, ke't qua nhu sau:
Bdng 7. Anh hudng eua vanadium liin51!sinh truongcityhoa cue
Chrysanthemum indicumL.
Nghi~m N6ng d() Chi6u caD Phat sinh H~s6 TInh trang
th(lc ( mg!1 ) ( em ) r~( % ) nhan cay VIII 0 5,51 100 4.75 ++ VII2 1.0 416 100 301 ++ VII3 2.5 2.28 100 1.49 + VII4 5.0 1.75 0 1.25 + VII, 10.0 1.57 0 1.26 + VII, 30.0 1.50 0 1.25 + VII, 50.0 1.34 0 1.03 + VII, 100.0 - - - -
(Ohi chu: +++: Cay t6t, ++: Cay tmng binh, +: Cay ye'u, -: Cay che't)
Chuong V. Vqe linh Vva taed~nggidi dqe eila chilosan chie'u X<;l 60
Qua bang ket qua, chung toi nh~n thily:
Tu n6ng a(l vanadium l,O mg/I tn) ai, cay b~t a~u bi (Ie ehe sinh tnldng. Chi~u cao cay cfing nhl1h~ so nhan giam thea chieu tang eua ndng d(j
vanadium, tham chi ngtlng pMt triGn hoan toan (nghi~mthuc VIJs,VIJ" VII,) va ehet (nghiem thuc VIJ,). nnh trang cay d cac nghi~m thue e6 xu 19 vanadium yeu: cay e6 mau xanha~m,dn e6i, la nho. Tai n6ng a(l 100mg/I, cay chet hoan toan sau 4tu~n nuoi ciy.
C5 n6ng a(l vanadium tu 5 Mn 50 mg/I, Sll phan h6a r~ bi (Ie ehe hoan
toano £)~1I r~ va dO'.l1l than diy khi tiep xuc v(jj m6i trt(ong bi chuy~n sang mau aen va khong phiit triGn n["la. He r~ Clla cay bj t5n thtWngd~n aen cay khong thG hut dinh Mong ngoai moi tnlong. f)i~ua6 gay ra ue
che sinh tntdng va phil tri&ndiy.
Tai n6ng a(l vanadium 2,5 mg/l, ehi~u eao cay, he s6 nhan giam xilp xi 50 %. C5 nghiem th(le nay (VII!,), ty I~ phiit sinh r~ la 100% nhllng r~
phiit triGn rilt kem. R~ dai 0,5 em, so voi a6i chung la 5- 8 em. f)~u r~ e6 mau aen. C5 nghiem thuc nay e6 hi~ntUOng hlnh thanh cac r~ con th(l sinh <1 cae a6t phia tren g~n ngQn. Cac r~ th(1 sinh nay VlWn ra khoang khong gian phia tren moi truong M hut dllOng khi, cho Mn khi cham phai moi truong c6 ehtla kim loai gay a(le thi bi t5n thuong va r~ th(1 sinh moi lai hlnh thanh. Tuy v~y, ai~u nay khong tao nen kha quan cho su s6ng etla cay. Cay v~n bi (Ieehe sinh u·udng 50 %. Nhu vay, li~u (Ieehe 50 %sinh trudng LG 50 (Limited Dose 50) etla vanadium u·en cay Cue la 2,5 mg/1.
8 - - r - - - ,
§ 5.51
6 -
0.00
VB 1 VB 2 VB 3 VB 4 VUS VU6 VU? VB8
Nghi~m thac
Biiu d8 7a. Anhhuangeila vanadiumlenchi€ucaD trung blnh cay hoa cue Chrysanthemum indicumL.
Chudng V. DQe /(nh V va Ide dl:'ng giiii dge eua chi/osan ehie'uX(l c 6 . 4.75 C~ -"c 4 3.01 '<0 ~ 1.49 1.25 1.26 1.25 .".:I: 2 1.03 0 0
YUI YU2 VU3 YU4 YU5 YU6 YU7 YUS
Nghiem th((c
Bi€u dt 7b. Anh huang cua vanadiumlenh~so'nhan eay hoa cue Chrysanthemum indieumL.
§: 150· 100 100 100 t<~ 100 . -"c .<;; 50· ,;;; -" a a a a a 0.. a .
VU 1 VU2 VU3 YU4 YU5 VU6 VU7 YUS
ghiem th((c
Bi€u dt 7e. Anh huiJng eua vanadium ten phdl sinh rl ciiy Jwa cue
Chrysanthemum indicumL.
61
Nhu' v~y,c6 th6kh~ng dinh vanadium la nguyen to' gay dQC cho cay Cuc
in vilro.6 1,0mgll b~tdfill c6 tac dung tYc che'. N6ng dQ 2,5 mg/lac che'50%
sinh tnldng.Titn6ng dQ 5 mgll Stl sinh [l1ldng cua cay bi (IC che' hoan toan do he r€ cua chung bj [5n lhtlong nghiem lrQng.
Chuong V. Dqc rinhvva racd~nggiai dqc clla chilosan chif!'u X(l 62
• I.S
e
III
/-flnh 20a. Anh huang clla vanadium len sl! sinh rruang
Chrysanthemum indicumL.
/-flnh 20b. Anh huang clla vanadium lenS'!sinh truong ciiy hoa cue
Chrysanthemum indicumL.
Chuang V. Dqe tfnh Vveltde d¥ng giiti dqe eita ehitosan ehie'uX(i 63 Thi nghi~mda khhg dinh r6 rang doc Hnh cua vanadium. EJitu dang 10
ng~i Ia hi~n nay, cae chilt lhai kim lo~i nang ltOng d6 c6 vanadium dang ngay cang gia tang, gay 0 nhi6m moi Wong sinh lMi, anh hl(dng de'n s((c khoe con ngt(oi. Do d6 nhitu nghien ccru giai dQc kim loai nang, lrong d6 giai phap khtY dQc kimlo~i nang lrong cac chilt thai nho cac hqp chill c6 hoal tlnh sinh hoc vi du hydratcarbon, dadu'qc tie'n hanh. MQI sa tac gia da cong ba kha nang t~o phcrc vdi kim lo~i nang cua chilOsan va dac biet chitosan khi duqc phan gilii thanh cac phan doan chitosan ng~n hdn, vdi ltOng luqng phan ltY nho hdn, c6 kha nang b~tkim lo~icao hdn. Do d6, chung loi tie'n hanh lIm hiliu kha nang
giai dQc kim lo~i n~ng vanadium lfen cay cuc Chrysanthemum indieum L.
duqcgay dQc vanadium nM chilosan elll machb~ngbcrcx~ gamma.