2. MATERIALS AND METHODS
-1098 ATCTATGAGG GTGCCCGTGT TTAAGGCTTT GGGGAGGTAT CTGGTAGGTT CATTGATAAT 1038 TTGTGTGAGG TTGAGGGCAT CAAGTTTAGA TTGTAGGATG GCTGGGGTGT TAAGCATGTT
-1038 TTGTGTGAGG TTGAGGGCAT CAAGTTTAGA TTGTAGGATG GCTGGGGTGT TAAGCATGTT -978 CCAGTTTAGG TCGCCTAGCA GCACAAGCTC TGAAGATAGA TGGGGGGCAA TCAGTTCACA -918 TATGGTGTTC AGAGCACAGC TGGGGGCAGA GGGTGGTCTA AAGCAGGCGG CAACGGTGAG -858 AGACTTGTTT TTAGAGAGGT GGATTTTTAA AAGTAGAAGT TCAAATTGTT TGGGTACAGA -798 CCTGGATAGT AGGACAGAAC TCTGCAGGCT ATCTTTGCAG TAGATTGCAA CACCGCCCCC -738 TTTGGCAGTT CTATCTTGTC TGAAAATGTT GTAGTTTGGA ATTATGTGTA TTATTATGAA -678 AATGTGACAC ACATAACCCT ATATGCCACC CTTCATGTAG GATTACATTT CCTTAATGAT -618 TTGGGGTGGC CTTATGAAGG GAGTCTGAAG GCCTTATAAG ATATACTTTG TTTAAAGTGT -558 GACCTGACAT GCTGACCAGA CCGATCACGA GTGTCTACGT GCTACATCGC ACGCATGTTG -498 ATTTTGTCCA CCCACACCAG ACGTAATCAG GACATGCAGG TTGAAATATC AAAACAAACT -438 CTGACCCAAC TATATTAATT TGGGGACATG TCGAAAAGTT CTAAACATGT ATGTCTATTT -378 AGCTAGCTAG TGTGCTGTTG CTGGCTAATT TGTCCTGGGA TATGAACATT GGGTTATTAT -318 TTTACCAGAA ATGCACAGGG TCCTCTACTC CGACAATTAA TCCACAGATA AAAGGGTAAA -258 CCAAGTTTGT TTCTAGTAAT CTCTCCTCCT TCAGACTTCT TCTTCTTCTT TGGACTTTAT -198 ATGGCAGTTA GCAACCAACT TTAAGGTGCA TTACCACCAC CAACTGGACT GGAGTGTGGA -138 CCTCTGTTCA TCGTTCAATC ACCCACAAGG GTATTTGCTC CTAAAAACCA ATGAGGAGAT -78 AGGAGAGGCG GGACTTGCAG CGCGTCAAGC ATCACAAATA GAACCAAGTT CTATTTTAGT -18 GCCTGGCTAT GCAGACGCGC ACGAGCAGTA TGTGTGTGTC TAATCATTGA ATAACATCGG 43 TGTAAATGTA TTTTGTAACG CTTGCGCATG TGCTGAGAGC GGTGTGGTCA GAATGTAACA 103 TGCCGATCAG AGCGCTGGCA CATGCGCCAT CGCAAGCATG TTGATTTTGT CCACCCACAC 163 CAGACGCGAT CAGGACACGC AGGTTGAAAT ATCAAAACGA ACTCTGAACC AACTATATTA 223 ATTTGGGGAC AGGTTGAAAA GCATTAAGTA TTTATGGCAA TTTAGCTAGC TAGCTTGCCT 283 TTGCTAGCTA ATTTGTCTTG GGACTGGACT GTGGACCTCA GTTCATCTTT CAATCACCCA 343 CGCGGGTATA TGCTCCTAAA AACCAAGGAG ACGGCACATT GGTAACATGC TTACCACACC 403 GCTCGTGTCG CATGCACGGG TGTTGCAAAA TAAATATACA CATACATGAT ATTCAATCAT 463 TACACCCACA CTGCTCGTGC ACGTCTGCGT AGCCAGGCGC TAAAATAGAA CTTGGTTCTA 523 TTCTGCTTGA CACGCTGCAA GTCCCGCCTC TCCCATCTCC TCATTGTTTT TTAGGAGCAT 583 ATACACGTGG GTGATTGAAA TATGAACTGA GGTTCACACG CCAGTCTAGT TGTTGGTAGT 643 AATGTACCTT AAAATTGGTT GCCAACCACC ATATAAAGTC CAAAGAAGAA GAAGCCTGAA 703 GGAGGATAAT TAATCTGTGG AATAATTGTT AGAGTAGAGG ATCTTGTGCA TTTCAGGTAA 763 AATAACATCC CATGACAAAT TAGATAGCAA CAGCAAGCTA GCTAAATTGC CATATATTTT 823 TTATGCTTTT CAACATGTCC CCGAATTAAT ATAGTTGGTG CAGAGTTTGT TTTGATATTT 883 CAACCTGCCT GTCCTGAATG CCTCTGGTGT TGGTGGACGC ACGCGTGCGA GGGGTCTGAT 943 CAGCATGTGT TTCCCTGCTG TGTCGCAGAT TCCTGAAGGG ACCTATTCTC CTTGTGGAAG 1003 CATTATGTAC TGCACCCACT TAAAGACGCT GCAACTGGTC GTCATGGCAA CGCTGTGGGT 1063 GAC
Figure 8: The sequence of the 2.2 kb PCR fragment of the csFSHβ subunit gene
The presumed partial sequence of the cDNA is shown in red and the sequence of the 1 kb csFSHβ fragment is shown in blue The first base at the 5’ end of the cDNA sequence (Suzuki and Hew, unpublished) has been designated +1. The sequence of FSH β subunit gene in black represents intron 1. The two possible exon-intron junctions are indicated by yellow and green arrow heads, respectively.