C Apoptosis
1 GGTATCAACG CAGCCTCCCG GCGGGAGGGA GAGGAGCAGG GAAGATGGCG GCTGTGGTGG 6 AGA ATGTAGT GAAGCTCCTT GGGGAACAGT ACTACAAAGA TGCCATGGAA CAGTGCCACA
121 ATTACAATGC CCGCCTCTGT GCTGAGCGTA GCGTGCGTCT GCCTTTCTTG GACTCACAGA 181 CTGGAGTAGC CCAGAGCAAT TGTTATATCT GGATGGAAAA GCGACACCGG GGACCAGGAT 241 TGGCCTCTGG ACAGTTGTAC TCCTACCCTG CCCGGCGCTG GCGTAAAAAG CGTCGAGCTC 301 ACCCACCTGA GGATCCCAGG CTTTCCTTTC CATCTATTAA ACCAGACACA GACCAGACCC 361 TGAAGAAAGA GGGGCTTATA TCTCAGGATG GCAGCAGTTT AGAGGCTCTA CTGCGT ACTG 421 ACCCTCTGGA GAAACGAGGT GCTCCAGATC CCCGTGTTGA TGATGACAGC CTGGGCGAGT 481 TTCCTGTCAC CAACAGTCGA GCACGGAAGC GGATCCTTGA ACCTGATGAC TTCCTAGATG 541 ATCTTGATGA TGAAGACTAT GAAGAAGATA CTCCAAAACG TCGGGGAAAG GGGAAATCCA 601 AGAGTAAGGG TGTGAGCAGT GCCCGGA AGA AACTGGATGC TTCCATCCTG GAGGACCGTG 661 ATAAGCCCTA TGCCTGTGAC ATTTGTGGAA AACGCTACAA GAATCGACCT TGCCTCAGTT 721 ACCACTATGC CTATTCCCAC CTGGCTGAGG AGGAGGGAGA GGACAAAGAA GACTCTCAAC 781 CACCTACTCC TGTTTCCCAG AGGTCTGAGG AGCAGAAATC CAAGAAAGGA CCTGATGGAT 841 TAGCCCTGCC CAACAACTAC TGTGACTTCT GCCTGGGAGA CTCAAAAATC AACAAGAAGA 901 CAGGGCAGCC TGAGGAGCTA GTGTCCTGTT CTGACTGTGG CCGCTCAGGG CACCCGTCCT 961 GCCTGCAGTT CACCCCCGTG ATGATGGCGG CTGTGAAGAC CTACCGCTGG CAGTGCATCG 1021 AGTGCAAGTG CTGCAACCTC TGCGGCACTT CGGA GAATGA CGACCAGCTG CTCTTCTGTG 1081 ATGACTGTGA CCGTGGCTAC CACATGTACT GTCTCACCTC ATCCATGTCG GAGCCTCCTG 1141 AAGGAAGTTG GAGCTGCCAC CTGTGTCTGG ATCTGCTGAA GGAGAAAGCG TCCATCTACC 1201 AGAACCAGAG CTCCTCCTGA TGTGCCACCC GGCTCCCCAC ACACCTAAGG CTGTTGCTCT 1261 CCTCTACCTT GGTTTTCATA CCCCTCTTCT TCTTCTTCTT TCACTCTGGT AGTTCTGCCC 1321 AACTGCCTTT GGCAACAGCA CAGGGAAGGT GGCAACTCTT GACTGCCTCT GGTCCCAAGC 1381 CCTCAGGGAG TAAGGAGCAG CATGCTGCCC CAGGCTGATC TGTGGGCCCA GCTTCTCTCT 1441 GCTCTCCAAG AAGTGCATTC ACTCTGCTTG CCTTGGGCCC AAGTCCCTGG TAATCACAGG 1501 GTTCAAATGG GTTCCTCTAA GAAGTATGAG AGCAGCTCAC TTGTCTCAAG CCTGGCCTAC 1561 CCCTCCTCCC CCTCTGGTGT CCAGAGTTTT ACCCCAGGGG TGAGCCAGGC CTAACCTTTG 1621 CTTGGAGCAC CTGGAGTGAT CAGACTGAGG TGGCACTTGC TAGGACCCTT TCCTACCCCT 1681 TGTTCTGCTT CACTTTGCCT CTGCCAAAGC AGTCCTGTGT CTTCTGTCAT GCTACATGGG 1741 GTCCTGTGCT TGCACTGTGA TGCTCTCAGG CACCTCCTGG CTCTGTCCTG TTCTGCCCAG 1801 TCCCACAAAG AGACAAGCAG CTTCACCTGC CCTTCCCGTG CTTGGCTGGC GCGCTCACAG 1861 GTGGTCTCTG GCAATCCAAA CATTTCCCAT CCTCAGACTT TTGAGTCTTC TGCCTCCTTC 1921 CTTGTTCCCT TTGGTTTTGT GGGGGAGAGG GACAATGTCA GGGGGCCCTG CCAGAAGCTT 1981 GGGGACCACA AGAAGTTGGA TAATGTGCCT GTTTTTTAAC TCGATAAAAA TGCCTACCTC 2041 CAAAATCCCC TTTTCGTTCT TCCTGGACCT GGGCATTCAG CCTCCTGCCC TTAACTGAAT 2101 CAGGAGCCTC TGCCTCCTAC TGTGTATCCT GGCTCCCAGG AGAGAGGATG GTCCCCTTTC 2161 CTTGCACACT AGCTAGCAGC TGGTAAAGTC TTCTTTCCCT GATTTTTGTT TCCTGCTTAG 2221 TGGCACTGAC ATTAAGTAGG AGGGGACAGT CCATGCCAGA ACACTCTGGA ATGGCCTTCC 2281 TCCTTGGCTG TGGGCAGGCC CTGACTTGTT TTCTGCAAAG TTGAGGCCCC TCCTCCTATC 2341 CTTAGTTCCT GTATCCAAAA CATTAGTAAG AATAAACATT TTTACACAGA AAAAAAAAAA 2401 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2461 AAAAAAAAAA AAAAAAAAAA AA
Nucleotide sequence of C. griseus Requiem cDNA. Underlined sequence denotes the open reading frame of REQUIEM.
1 MAAVVENVVK LLGEQYYKDA MEQCHNYNAR LCAERSVRLP FLDSQTGVAQ SNCYIWMEKR 61 HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP DTDQTLKKEG LISQDGSSLE 61 HRGPGLASGQ LYSYPARRWR KKRRAHPPED PRLSFPSIKP DTDQTLKKEG LISQDGSSLE 121 ALLRTDPLEK RGAPDPRVDD DSLGEFPVTN SRARKRILEP DDFLDDLDDE DYEEDTPKRR 181 GKGKSKSKGV SSARKKLDAS ILEDRDKPYA CDICGKRYKN RPCLSYHYAY SHLAEEEGED
Zinc finger C2H2-type domain 241 KEDSQPPTPV SQRSEEQKSK KGPDGLALPN NYCDFCLGDS KINKKTGQPE ELVSCSDCGR
Zinc finger PHD-type domain 1 301 SGHPSCLQFT PVMMAAVKTY RWQCIECKCC NLCGTSENDD QLLFCDDCDR GYHMYCLTSS
Zinc finger PHD-type domain 2 361 MSEPPEGSWS CHLCLDLLKE KASIYQ N QSS S
Amino acid sequence of C. griseus REQUIEM protein. The zinc finger C2H2-type domain is highlighted in dark grey while the zinc finger PHD-type domains are highlighted in light grey . There is a potential N-glycosylation site at Asn387.
CHO 1 MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKRMouse 1 MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKR Mouse 1 MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKR Human 1 MAAVVENVVKLLGEQYYKDAMEQCHNYNARLCAERSVRLPFLDSQTGVAQSNCYIWMEKR CHO 61 HRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLE Mouse 61 HRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLE Human 61 HRGPGLASGQLYSYPARRWRKKRRAHPPEDPRLSFPSIKPDTDQTLKKEGLISQDGSSLE CHO 121 ALLRTDPLEKRGAPDPRVDDDSLGEFPVTNSRARKRILEPDDFLDDLDDEDYEEDTPKRR Mouse 121 ALLRTDPLEKRGAPDPRVDDDSLGEFPVSNSRARKRIIEPDDFLDDLDDEDYEEDTPKRR Human 121 ALLRTDPLEKRGAPDPRVDDDSLGEFPVTNSRARKRILEPDDFLDDLDDEDYEEDTPKRR CHO 181 GKGKSKSKGVSSARKKLDASILEDRDKPYACDICGKRYKNRPCLSYHYAYSHLAEEEGED Mouse 181 GKGKSKSKGVSSARKKLDASILEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGED Human 181 GKGKSKGKGVGSARKKLDASILEDRDKPYACDICGKRYKNRPGLSYHYAHSHLAEEEGED CHO 241 KEDSQPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGR Mouse 241 KEDSRPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGR Human 241 KEDSQPPTPVSQRSEEQKSKKGPDGLALPNNYCDFCLGDSKINKKTGQPEELVSCSDCGR CHO 301 SGHPSCLQFTPVMMAAVKTYRWQCIECKCCNLCGTSENDDQLLFCDDCDRGYHMYCLTSS Mouse 301 SGHPSCLQFTPVMMAAVKTYRWQCIECKCCNLCGTSENDDQLLFCDDCDRGYHMYCLTPS Human 301 SGHPSCLQFTPVMMAAVKTYRWQCIECKCCNICGTSENDDQLLFCDDCDRGYHMYCLTPS CHO 361 MSEPPEGSWSCHLCLDLLKEKASIYQNQSSS Mouse 361 MSEPPEGSWSCHLCLDLLKEKASIYQNQNSS Human 361 MSEPPEGSWSCHLCLDLLKEKASIYQNQNSS
Alignment of amino acid sequences of C. griseus, M. musculus (NM_011262.1) and