κb inhibitor suppresses ark5 expression in an htlv 1 infected t cell line
Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
Ngày tải lên :
13/08/2014, 09:21
... of HTLV- 1- infected T- cells Constitutive tyrosine phosphorylation of Stat3 and Stat5 in HTLV- 1- infected T- cell lines We first examined HTLV- 1- infected T- cell lines [MT-2, HUT -10 2 and ED-40 515 (-)] ... Western blot Actin expression served as a loading control Figure T- cell lines Constitutive activation of Stat3 and Stat5 in HTLV- 1- infected Constitutive activation of Stat3 and Stat5 in HTLV- 1infected ... Stat3 and Stat5 by inhibiting three Jak kinases in HTLV- 1- infected T- cell lines Figure HTLV- 1- infected T- cell lines and primary ATL cells AG490 inhibits constitutive activation of Jak and Stat in...
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Báo cáo y học: "Severe hypercalcaemia and lymphoma in an HTLV-1 positive Jamaican woman: a case report" pot
Ngày tải lên :
11/08/2014, 10:23
... http://www.jmedicalcasereports.com/content /1/ 1/56 Competing interests The author(s) declare that they have no competing interests Authors' contributions VL, EK and TJA were all involved in managing the case and in preparing ... of HTLV- 1- induced acute adult T cell leukaemia/lymphoma (ATLL) Discussion ATLL is an aggressive malignancy that is aetiologically linked with the infection caused by HTLV- 1[ 1] HTLV- 1 infection ... opportunistic protozoal and fungal infections HTLV1 carriers have high rates of Strongyloides stercoralis infection [12 ] In ATLL this gut pathogen is often associated with hyperinfection and fatal...
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báo cáo khoa học: " High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-κB-complex-dependent gene expression in human heart failure" pps
Ngày tải lên :
11/08/2014, 12:20
... performed and western blotted for p53, STAT3 and RELA (f) Stat3-/- MEF cells and Stat3-/- MEF cells that were re-constituted with wild-type Stat3 were treated with or without DFX and quantification ... binds RELA through the RELA transactivation domain Purified recombinant GST-RELA peptides (amino terminus, DNA binding domain; carboxyl terminus, transactivation domain; in the left panel, the ... Page 13 of 14 10 11 Competing interests The authors declare that they have no competing interests Authors' contributions MKC and RF carried out the cellular, genetic and immunoprecipitation experiments...
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báo cáo khoa học: " Differential gene expression in an elite hybrid rice cultivar (Oryza sativa, L) and its parental lines based on SAGE data" doc
Ngày tải lên :
12/08/2014, 05:20
... 66.00 14 .00 8.00 2 51 3706 224 200 3473 246 9 311 _Chr07 _17 12 GATCCGTCTC 9 311 _Chr06 _15 45 GTACTGTCTG 9 311 _Chr03 _14 01 TTCCCCCATT 9 311 _Chr05_0842 CTGTATTACT Down-Regulated Tags (>2-fold) 9 311 _Chr 11_ 0807 ... 9 311 _Chr 11_ 0807 GAATATTGGA 9 311 _Chr10_ 218 5 TATCATTACA 9 311 _Chr07 _12 31 CACATAAATT 13 13 11 41 19 47 47 55 22 94 7.23 3.44 2.93 2 .14 12 88 249 2 61 1030 12 38 3 61 150 994 40 38 43 16 9 26 19 7 .17 5.50 5.33 ... microarray data Gene Model Tag Na Up-Regulated Tags (≥2-fold) 9 311 _Chr08_ 215 6 GATTTGTATA 9 311 _Chr06 _15 23 TCATTTCAGT 9 311 _Chr06 _11 42 ATCTGTTGCT Ratiob Tag Number Pa La Microarray Signal Na Pa 0 33 14 66.00...
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Tài liệu Báo cáo khoa học: Expression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum docx
Ngày tải lên :
19/02/2014, 16:20
... determined incubating the purified protein at temperatures from 25 to 70 °C in 50 mm sodium acetate for 30 and then measuring the remaining enzymatic activity adding mutan For optimum pH determination ... and perhaps between the two biotypes In connection with this idea, it is worth mentioning that both strains display clear distinctive host range and antagonistic abilities in controlled assays ... mutan Experimental procedures Strains and culture conditions T asperellum CECT20539, T harzianum CECT2 413 and Streptococcus mutans CECT4034 were obtained from the Spanish Type Culture Collection...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
Ngày tải lên :
31/03/2014, 07:20
... C-terminus of rat VRL -1 It is important to mention that this antibody also detects the mouse variant [11 ] The anti-VRL -1 antibody specifically detected a protein band at 80 kDa (Fig 1) This band ... 5¢-ACTCGGTGC TGGAGATCATC-3¢ and VRL -1/ 1897R: 5¢-TGAGAAG GACGTAGGCCAAC-3¢ to obtain fragment C with 836 bp; rVRL -1 16 18F: 5¢-TTCCTGCTGGTCTACCTG GT-3¢ and rVRL -1 2251R: 5¢-CTTCCTCTGAGGCACT GTTC-3¢ to ... show that the F -11 cells not only express the rat VRL -1, but also the mouse VRL -1 and that the mouse variant is derived from the F -11 parental cell line N18TG2 This finding is of interest when...
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báo cáo hóa học: " Endotoxin-induced cytokine and chemokine expression in the HIV-1 transgenic rat" docx
Ngày tải lên :
19/06/2014, 22:20
... Interleukin 12 ; IL -13 : Interleukin 13 ; IL -13 r 1: Interleukin 13 receptor alpha 1; IL -15 : Interleukin 15 ; IL -16 : 19 ` Interleukin 16 ; IL -17 B: Interleukin 17 B; IL -18 : Interleukin 18 ; IL-1F5: Interleukin ... greater in the HIV-1Tg rats than in the F344 In the ET HIV-1Tg and F344 (LL+L) rats in the spleen, the LPSinduced increase in pro-inflammatory cytokines was diminished and that of the anti-inflammatory ... Institutional Animal Care and Use Committee; IFN-β: Interferon beta; IFN-γ: Interferon Gamma; IL -10 : Interleukin 10 ; IL -10 rα: Interleukin 10 receptor, alpha; IL -11 : Interleukin 11 ; IL -12 : Interleukin...
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Tự học Indesign CS2 : In ấn part 1 ppt
Ngày tải lên :
25/07/2014, 10:20
... Các trang in Trang 11 đến trang cuối t i liệu T t trang trở lên bao gồm trang 11 Chỉ trang 11 T t trang trở lên bao gồm trang 11 T t trang t trang thứ 11 đến trang cuối t i liệu Trang 1, cộng ... nhìn thấy t t tài liệu sách Print thấy, sách Các t y chọn trang Các t y chọn trang hộp thoại Print • All : in t t trang t i liệu Đây thi t lập mặc định • Range : xác định phạm vi trang để in t i ... với t T ộ ới trang t đế đến Trang 1, cộng với trang t đến T t trang section có nhãn “Sec1” Trang đánh số ( không thi t trang thứ section g g g ) section có nhãn “Sec2:” Trang đánh số section...
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Báo cáo y học: " Substitution of the Rev-response element in an HIV-1-based gene delivery system with that of SIVmac239 allows efficient delivery of Rev M10 into T-lymphocytes" ppsx
Ngày tải lên :
10/08/2014, 05:21
... marginal increase in titer over that obtained in the absence of any 'transport' protein expression construct These results suggest that both packaging and gene transfer vector constructs must contain ... pMD.G and pCMVtat The titers of the resultant virus stocks were determined using Jurkat T- cells The SEAP-adjusted vector titers are summarized in Table Attempts to package an HIV -1 RRE containing ... positivity determined by flow cytometry The bottom panel depicts mean p24 levels in the vector containing supernatants The titers and p24 levels were normalized to SEAP activity present in the vector...
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báo cáo khoa học: " All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein" pdf
Ngày tải lên :
10/08/2014, 10:20
... Mechanisms of resistance to imatinib mesylate in gastrointestinal stromal tumors and activity of the PKC 412 inhibitor against imatinib-resistant mutants Gastroenterology 2005, 12 8:270-279 19 Collins ... Upon the importance of KIT protein, we examined whether ATRA can suppress KIT activity in GIST -T1 cells We treated GIST -T1 cells with 18 0 μM ATRA for the indicated duration Total cell lysates ... Ebeling P, Zeth M, et al: Resection of residual disease in patients with metastatic gastrointestinal stromal tumors responding to treatment with imatinib Int J Cancer 2005, 11 7: 316 -325 DeMatteo...
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báo cáo khoa học: "Evaluation of HER2 and p53 expression in predicting response to docetaxel-based first-line chemotherapy in advanced breast cancer" docx
Ngày tải lên :
10/08/2014, 10:21
... the tumor cells (1+ ); complete staining of the membrane with weak or moderate intensity in more than 10 % of the neoplastic cells (2+); and strong staining in more than 10 % (3+) Cases with or 1+ ... Positive 22 17 Negative 13 Positive 23 16 ER expression ER expression 17 15 I-IIA 17 15 IIB-III 16 10 17 21 Positive” Ki67 Negative 22 15 Positive 14 10 CR+PR 15 11 SD+PD 21 14 13 23 18 21 18 15 ... FDArecognized test, the Herceptest™ (DAKO), describes four categories: no staining, or weak staining in fewer than 10 % of the tumor cells (0); weak staining in part of the membrane in more than 10 % of the...
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Báo cáo y học: "Evolution of antibody landscape and viral envelope escape in an HIV-1 CRF02_AG infected patient with 4E10-like antibodies" docx
Ngày tải lên :
12/08/2014, 23:22
... corresponding neutralization epitopes may be restricted to the conformational Env context of particular virus variants that induce these antibodies in natural infection [11 -14 ] In the present study ... Competing interests The authors declare that they have no competing interests Authors' contributions TD performed the majority of the experimental work and the data analysis and drafted the manuscript ... A: Anticardiolipin, anti-beta(2)glycoprotein I, antiprothrombin antibodies, and lupus anticoagulant in patients with systemic lupus erythematosus with a history of thrombosis J Rheumatol 19 97,...
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Báo cáo y học: "ATF3, an HTLV-1 bZip factor binding protein, promotes proliferation of adult T-cell leukemia cells" docx
Ngày tải lên :
13/08/2014, 01:20
... represent open reading frame (ORF) Two distinct ATF3 transcripts that encode the same ORF are reported[32] (B) ATF3 mRNA from P1 and P2 transcripts in HTLV- 1 infected cell lines and ATL cell lines ... suppresses Taxmediated ATF/CRE-dependent transcription both of cellular genes and the HTLV- 1 LTR ATF3 has growth promoting activity in ATL cells To investigate the functional significance of ATF3 expression ... necessary for their interaction The ATF3 promoter is constitutively activated in ATL cell lines Next, we checked the expression level of ATF3 mRNA and protein in ATL cell lines The ATF3 gene has two Page...
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Báo cáo y học: " MicroRNA miR-146a and further oncogenesis-related cellular microRNAs are dysregulated in HTLV-1-transformed T lymphocytes" ppsx
Ngày tải lên :
13/08/2014, 05:21
... AFF1 RDX RIT1 USP 31 ARHGEF1 SERTAD2 24 14 6a 2 1 2 1 1 1 1 1 1 1 1 1 3 2 3 1 1 1 1 1 1 1 1 1 3 2 2 2 1 1 2 2 1 1 1 1 Gene ontology (GO) terms for biological processes 15 51 2 41, 2, 14 6a1, 15 53 211 ,2, ... 2 41, 2 15 53 14 6a3 213 2 41, 2,3, 15 53 14 6a1 1, 2, 15 51, 2,3 24 2 41 211 ,2, 2 41, 2, 14 6a3 14 6a1,3 211 , 2 41 243 243, 14 6a1,2, 15 51 1553 211 , 2 41, 15 5 213 15 51 signal transduction cell adhesion regulation ... Using mutated forms of Tax and the coexpression of a dominant active NF -κB inhibitor, we were able to pinpoint the transactivation as being mediated via NF -κB These findings are in line with...
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Báo cáo y học: " Increased proviral load in HTLV-1-infected patients with rheumatoid arthritis or connective tissue disease" potx
Ngày tải lên :
13/08/2014, 13:20
... disease and from HAM/TSP with in the peripheral blood from HTLV- 1infected patientsor asymptomatic controls HTLV- 11 HTLV- 1 proviral load in the peripheral blood from HTLV- 1- infected patients with RA ... lymphocytes isolated from patients with rheumatoid arthritis Arthritis Rheum 19 88, 31: 1230 -12 38 Panayi GS, Lanchbury JS, Kingsley GH: The importance of the T cell in initiating and maintaining the ... etiological factors might secondarily enhance HTLV- 1 proviral load through cell activation, with subsequent migration of HTLV- 1- infected cells into the joint or CNS The accumulation of HTLV- 1infected lymphocytes...
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Recommendations for Use of Antiretroviral Drugs in Pregnant HIV-1-Infected Women for Maternal Health and Interventions to Reduce Perinatal HIV Transmission in the United States pdf
Ngày tải lên :
05/03/2014, 13:20
... ART for their own health and those who not require therapy but opt to start ART to prevent sexual transmission It is important to recognize that no single method (including treatment of the infected ... Panel recommendations Other guidelines These guidelines focus on HIV -infected pregnant women and their infants Other guidelines outline the use of ARV agents in non-pregnant HIV -infected adults ... enrolled 1, 763 HIV -infected participants with CD 4cell counts between 350 and 550 cells/mm3 and their HIV-uninfected partners The infected participants were randomized to immediate initiation of ART...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... GCCGTGTGCGTGC-3¢, antisense 5¢-ACTTTCTTCATT TCCACCTTTGCC-3¢; MnSOD [40], sense 5¢-CTTCA GCCTGCACTGAAGTTCAAT-3¢, antisense 5¢-CTGAA GGTAGTAAGCGTGCTCCC-3¢; 36B4, sense 5¢-GTGA TGTGCAGCTGATCAAGACT-3¢, ... soluble intracellular antioxidant defences but not ROS flux in the studied q0 cells, and that there are cell line- to -cell line variations in intracellular antioxidant defences and ROS homeostasis In ... 10 4 10 3 10 4 10 1 10 2 FL1-H 10 3 10 4 10 1 10 2 FL1-H 10 3 10 4 60 40 Counts 20 80 10 0 60 40 20 0 10 0 10 2 FL1-H 10 0 Counts 60 40 20 Counts Lung 10 1 80 10 0 10 0 80 10 0 10 0 60 80 10 0 20 0 Counts 10 2 FL1-H...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
Ngày tải lên :
17/03/2014, 23:20
... with isotype control antibody and stained with the fluorescent antibody against the other protein, on ice The double-stained cells were analyzed for cocapping by a Zeiss Axiovert 13 5 TV invert ... with other membrane microdomains (e.g those accumulating TrfR) at the surface of the T cell lines investigated Supporting the detergent-resistance data, CD25 and CD48 also exhibited a detectable, ... enhanced binding of anti-(P-tyrSTAT3) Ig (more than fourfold) and anti-(PtyrSTAT5) Ig (more than sixfold), respectively, relative to their basal level (detected in control, unstimulated T cells) This...
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Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
Ngày tải lên :
23/03/2014, 13:20
... in L-MAT cell apoptosis Results Inhibition of TCDD-induced L-MAT cell apoptosis by rottlerin, an nPKC inhibitor 12 -O-Tetradecanoyl phorbol -13 -acetate (TPA) was used in combination with TCDD to ... not treated with TCDD (as indicated in the text), were fractionated Then, 40 lg of the particulate (membrane + cytoskeleton) fraction protein was examined by Western blotting to determine whether ... fractionating L-MAT cells into cytosol and particulate fractions and then examining the translocation of each of the nPKC isoforms in L-MAT cells treated with TCDD (by 905 PKCh in TCDD-induced signaling...
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