zas zinc finger proteins the other κb binding protein family

... the phospholipid and membrane binding of the other members of the PEBP family Consequently, the binding characteristics of PEBP proteins, including those of the binding of IC to phospholipid ... liposomes No binding of d1–7IC to PtdIns(3)P ⁄ PC was detected, and the KD value of d1–7IC binding to PtdIns(3,4)P2 ⁄ PC was increased 13-fold For the other liposomes, the KD values of the mutant ... the binding affinity and specificity (Table 2) clearly A suggest that the CPY -binding sites [8] and the phospholipid recognition site of IC overlap, and that the N-terminal segment at the CPY-binding...

Ngày tải lên: 19/02/2014, 05:20

... defend against infection by S enterica and possibly other bacterial pathogens [15] Although no other proteins from the guanylate -binding protein family are known to exist as more than one splice ... variants, which form two different proteins [16] These two proteins differ with respect to the presence or absence of the C-terminal 97 amino acids including the C-terminal geranylgeranylation ... oligomers rather than monomers Human guanylate binding protein (hGBP5) belongs to the family of interferon-c-induced p65 GTPases, which has seven members in the human genome [5] This family of...

Ngày tải lên: 15/03/2014, 10:20

... to or lower than the corresponding ones for other nonthermophilic proteins Therefore, there seems to be no shift in the stability curve with the hyperthermophilic HUTmar protein The average specific ... in the absence of DNA and not very clearly defined in the X-ray picture of the protein (Fig 1) We report here the results of an extensive thermalstability study of a hyperthermophilic protein, the ... proton transfer between the protein and the buffer, it is reasonable to assume that the heat capacities of both the native and unfolded states depend upon neither the pH nor the protein Ó FEBS 2004...

Ngày tải lên: 23/03/2014, 12:20

... exists between the amide group of amino acid 35 and the c-phosphate in the mutant proteins, in the GTPcS-complex the free energy differences DG° and thus the equilibrium populations of the three stereoisomers ... on the presence of Ras, but may be also due to other protein impurities occurring in low concentrations in the Ras preparations In contrast to the situation observed for wild-type protein in the ... Influence of the nucleotide bound on the Ras conformational states 31 P NMR spectroscopy allows us to probe the conformational states of nucleotide -binding proteins, such as Ras-related proteins, ...

Ngày tải lên: 30/03/2014, 09:20

... only the area of interest, the medial side of the left knee The surgery began with a small skin incision starting from the distal femur and extending to the proximal tibia on the medial side of the ... knee The subcutaneous tissue was dissected throughout the length of the skin incision The deep fascia that connects the parapatellar fascia to the biceps femoris was separated exposing the medial ... of the joint including the medial collateral ligament (MCL) and the joint capsule In the partial meniscectomy (PMX) surgical paradigm both the MCL and the joint capsule were incised to reveal the...

Ngày tải lên: 09/08/2014, 01:22

... and MS2-fusion proteins, these molecules will tether to the same MS2 RNA -binding site (MS2BS) on the mRNA This is a native property of the bacteriophage coat protein that binds the MS2BS hairpin ... and contain several other components such as ganglioside GM1, glycophosphatidylinositol-anchored (GPI-anchored) proteins, tyrosine kinases of the Src family and others Because the Gag and Staufen1 ... In the first case the VN is expressed as a fusion MS2-VN protein and can tether to the RNA reporter in proximity to the psi sequence (Figure 7A) hGag-VC will interact with the psi domain of the...

Ngày tải lên: 12/08/2014, 23:23

... functionally replace each other in planta when expressed in the same cells and localized at the same side of the cell [16,19] The second major PIN gene subfamily encodes proteins with the central hydrophilic ... specific PIN proteins in auxin management at the cellular level The low pH in the apoplast (the region outside the cell membrane comprising the plant cell wall) is maintained by the activity of the plasma ... homeostasis [13] The precise origin of PIN proteins in the evolutionary history of plants is not known The basal split of the Viridiplantae - that is, the separation of the Streptophyta (the clade containing...

Ngày tải lên: 09/08/2014, 20:21

... MCM proteins [57-59] Frontiers Now that roles for ORC proteins have been established at other points in the cell cycle than simply the G1/S boundary, it is of primary interest to determine the ... between Orc1, Orc2, Orc4, and Orc5 proteins and origin DNA [44] Given the lack of such conserved origin sequences in other eukaryotes, it is not surprising that other means by which ORC association ... in other organisms, the sequential association of the ORC, Cdc6, Cdt1, and Mcm2-7 at origins appears to be conserved in other eukaryotes, including S pombe and Xenopus (reviewed in [23]) Furthermore,...

Ngày tải lên: 14/08/2014, 21:20

... myosin II binding proteins (Mlc1, Mlc2) in yeast were among the 28 up-regulated proteins (P < 0.0006) To globally delineate the impact of Gis2p expression on the fate of mRNA targets and proteins ... In the case of the Pumilio/FBF (PUF) family proteins in yeast, flies and human, the PUF RNA -binding domain is well-conserved as is the sequence motif to which this domain binds; however, the ... previous detailed analysis of the RNA- and DNA -binding properties of ZnF proteins Analogous to GWW repeats, certain classes of DNA -binding ZnF proteins contact the three adjacent nucleotides...

Ngày tải lên: 09/08/2014, 22:23

... infection inocula Figure shows the expression patterns for genes encoding DEAD box proteins, and other genes encoding RNA helicases and RNA binding proteins, as assigned by the gene ontology database ... yeast gene [26] Other genes encoding proteins with RNA splicing /binding and RNA transport activity were also up regulated during this period, including the methylated mRNA cap binding proteins EIF4G1 ... is correct, then the selective targeting of such DEAD box factors might offer another means of ending HIV latency and for depleting latent HIV reservoirs The DEAD box proteins and other RNA helicases...

Ngày tải lên: 13/08/2014, 13:20

... [10] Although the occurrence of family 33 CBPs has been known for some time [23], the present results provide only the third demonstration of the accessory function of these proteins The effect ... the real natural substrates of the CBP proteins differ from the substrates used here and in other studies There may exist composite natural chitinous substrates that are more susceptible to the ... from S marcescens The fact that the longer part of the substrate extends towards the + side of the catalytic centre is compatible with the notion that the C-terminal substrate -binding domains are...

Ngày tải lên: 18/02/2014, 08:20

... of the aglycone -binding site, probably subsite +1, because they influence the binding of the first glucose unit of the aglycone On the other hand, M453 may be positioned in subsite +2 because these ... non-reducing end of the aglycone was called the ‘first glucose unit’ and the other glucose units were sequentially named in the direction of the reducing end of the Fig Binding energies for the interactions ... the aglycone -binding site of Sfbgly and interact with the initial pentyl moiety formed by methylene groups to of the alkyl-type aglycones On the other hand, the binding of alkyl moieties in the...

Ngày tải lên: 18/02/2014, 17:20

... suggests a possible role in the enzymatic reaction which will be discussed later The structure of the active site, the type of protein ligands, the zinc- binding motif, the presence of a water FEBS ... formation Sections (6 A thick) of the protein surface that illustrate the shape and size of the active site The sphere represents the zinc ion (A) The main body of the active site cavity is formed ... with related proteins BcZBP shares significant sequence similarities with the two proteins of known structure from the Pfam02585 family (Fig 6), namely TT1542 (1UAN.pdb) from Thermus thermophilus...

Ngày tải lên: 19/02/2014, 00:20

... The two proteins are found in the same cells and are indistinguishable in terms of DNA -binding specificity (Fig 5A,B), and both proteins bound independently to the GA motif (Fig 5C) All Sp proteins ... or Sp3 to regulate the expression of the promoter of the gene for p21Waf1/Cip1 [28,29] Therefore, we examined the effect of p300 on the promoter activity of the Dnmt1 gene in the presence of pCMV-Sp1 ... investigated the binding to various GST–p300 fusion proteins by the GST-pull down assay [25] Only the C-terminal region of p300 (amino acids 1572–2414), which included the C/H region and E1Abinding...

Ngày tải lên: 22/02/2014, 07:20

... experiments Binding of rFnBPB N2N3 to immobilized Fn fragments The binding site in Fn for S aureus FnBPs is located in the N-terminus [20] However, another binding site in the C-terminal gelatin -binding ... and may therefore play an important role in the ‘docking’ step of Fg binding In order to determine whether the predicted ligandbinding trench plays a role in the interaction between the A domain ... important role in the binding of the A domain of FnBPB to Fn Binding of rFnBPB N2 and rFnBPB N3 to immobilized Fn In order to localize the Fn -binding site in the N2N3 subdomain of FnBPB, the recombinant...

Ngày tải lên: 06/03/2014, 00:20

... in the binding of the ligand to the other repeats that share a common epitope Other inhibition mechanisms cannot be excluded; among these, there is the possibility that, through the effect of the ... staphylococcal Fn -binding proteins, besides promoting the production of LIBS antibodies, has the potential to elicit the generation of blocking antibodies Thus, the repetitive motifs of Fn -binding proteins ... both proteins in the presence or absence of Fn (or the NTD), all of the mAbs showed strong anti-LIBS activity However, these results not rule out the possibility that the immune system of the...

Ngày tải lên: 06/03/2014, 22:21

... anesthetic action Results Binding of the volatile anesthetic halothane to the hydrophobic core of porcine odorant binding protein The binding of halothane to the porcine odorant binding protein ... General anesthetic binding to a b-barrel protein binding protein (Kd ¼ 100 ± 10 lm) is therefore comparable to the findings in the latter two studies For both anesthetics, the free energy of binding ... odorant binding protein (pOBP) with isoflurane, showing the calorimetric response as successive injections of ligand are added to the reaction cell The lower panel depicts the binding isotherm of the...

Ngày tải lên: 07/03/2014, 16:20

... certain amino acids in the intrarepeat loops abrogates the binding of some B subunits but not others [13,28] The results here de®ne two distinct PP2A binding domains in the B subunits that are ... Schematic summary of the binding properties of the B56a fragments The empty bar represents full-length B56a or its fragments, and the gray boxes represent the deduced A subunit -binding domains (B) ... included in the pro®les The binding of the two conserved regions from B and PR72 to GST-A To test whether these two conserved regions of B subunits found in B/B55/CDC55 and PR72/B¢¢ family members...

Ngày tải lên: 08/03/2014, 10:20

... into the evolution of the molecular sites involved in the two modes of aglycone binding The amino acid sequence of Zm-p60.1 was compared with the sequences of 22 other members of the GH1 family ... F200K and P2 Furthermore, the F193A and W373K mutations not result in any change in the thermostability of the enzyme (Table S2), and thermal unfolding of the wild-type and both the F193A and W373K ... separated from other proteins in their respective preparations by native PAGE using 10% (w ⁄ v) gels [33] They were then subjected to in-gel activity staining (zymography) by incubating the gels for...

Ngày tải lên: 16/03/2014, 04:20

... of the minor light-harvesting protein, CP29, at the Thr6 residue [43] However, the direct substrates of these two protein kinases remain to be determined The common structural features of all the ... together with two copies of the minor chlorophyll a ⁄ b -binding (Cab) proteins CP29 and CP26, with each pair symmetrically related by the twofold axis of the core dimer This is the basic highly conserved ... transition therefore occurs in response to over-reduction of the PQ pool, resulting in the activation of specific thylakoid-bound kinase(s) This activation involves the binding of PQH2 to the quinol-binding...

Ngày tải lên: 16/03/2014, 06:20