yy1 s oncogenic regulation in prostate cancer

... Cell Adhesion Proteins in Prostate Cancer 617 Chapter 25 Integrins in Prostate Cancer Invasion and Metastasis 619 Paulynn Chin Suyin, Joanne Louise Dickinson and Adele Frances Holloway Chapter ... re‐ sponses would probably elicit similar results 4.4 Stemness in PCa: MFV as a “Stem Cell Virus” 4.4.1 Prostate Cancer stem Cell or Cells ? Essential aspects of PCa have been here discussed ... Advances in Prostate Cancer type of viruses have been shown to be capable of persisting in infected patients and ani‐ mals for several months/years [174] Furthermore and as discussed in the next section,...

Ngày tải lên: 22/03/2014, 22:20

... findings of a pilot study, investigating urinary exosomes in prostate cancer patients We had two main aims in the study; firstly to assess the feasibility of using urine as an exosome source in ... decrease in the amount of exosomes present Prostate Cancer cell lines produce typical exosomes, positive for prostate and cancer- associated antigens Two prostate cancer cell lines were maintained in ... functions for cancer exosomes, assisting cancers evade immune attack [21-24] Cancer exosomes may also contribute to angiogenic processes [25], may disseminate metastatic potential in certain settings...

Ngày tải lên: 18/06/2014, 15:20

... for prostate cancer, it has poor specificity to cancer PSA is highly expressed in noncancerous prostatic tissues as well as in cancerous tissues [18-20] as shown in Figure 2B in the present study ... specimens of two prostate cancer cases other cases, peptide-specificity was assessed by ELISPOT assay CTLs of case could release interferon-γ in response to AMACR1, and peptides, but not in response ... 0.05 was considered to indicate statistical significance Results AMACR Expression in Normal Tissues, Prostate Cancer Cell Lines and Cancer Tissues First the expression profile of AMACR in normal...

Ngày tải lên: 18/06/2014, 15:20

... virus (XMRV) is a member of the gammaretrovirus family that was first detected in human prostate tumors [1] Although initial studies supported the presence of XMRV in prostate cancer tissues [2-4], ... immunoblotting using anti-ApoC29 to assist in A3G identification in the gel for mass spectrometry The gel bands corresponding to A3G protein were excised and were subjected to in- gel trypsin digestion ... its precursor Gag in our experiment (Figure 3A) Subsequently, we analyzed A3G expression in these cells by western blot analysis Intriguingly, XMRV infection resulted in a substantial downregulation...

Ngày tải lên: 18/06/2014, 18:20

... receptors in cells and tissues Frozen sections of human prostate tissues (normal and tumour) were sectioned at a thickness of μm using a cryostat The sections were mounted on super frost plus microscope ... the same was clearly demonstrated Over-expression of TGase-4 in prostate cancer cells diminishes the action of MDA-7/IL-24 in prostate cancer cells -Motility assays Here, an ECIS based wounding ... human prostate tissues [14] As part of our continuing studies to investigate proteins interacting with TGase-4 using immunoprecipitation of proteins from the prostate gland, we identified a small...

Ngày tải lên: 18/06/2014, 19:20

... receptors in cells and tissues Frozen sections of human prostate tissues (normal and tumour) were sectioned at a thickness of μm using a cryostat The sections were mounted on super frost plus microscope ... the same was clearly demonstrated Over-expression of TGase-4 in prostate cancer cells diminishes the action of MDA-7/IL-24 in prostate cancer cells -Motility assays Here, an ECIS based wounding ... human prostate tissues [14] As part of our continuing studies to investigate proteins interacting with TGase-4 using immunoprecipitation of proteins from the prostate gland, we identified a small...

Ngày tải lên: 20/06/2014, 03:20

... (TCP) is modelled as a Poisson distribution [39] In such a geometrical setting it is defined as: TCP  e  n i SFi i SF i is the surviving fraction within the single subvolume with the running index ... whole prostate dose, low g50 (CN + definition), Fowler s a/b In contrast, one may assume a parameter set with slightly less optimal conditions for a SIB Table summarizes the results when assuming ... dose concept of the M D Anderson trial [1]), a/b is low and g50 is less steep than the corresponding ASTRO value Based on these assumptions the gain of a SIB is low, as the initial TCP is again...

Ngày tải lên: 09/08/2014, 08:22

... to gain insight into a combination effect on apoptosis induction we subsequently assessed DNA-fragmentation by using flow cytometry and caspase-activation by using Western blot analysis In PC3 ... Bcl-2 proteins As shown in previous investigations, ErPC3 induces apoptosis via the intrinsic mitochondrial pathway [16] We therefore next examined whether the differences in apoptosis sensitivity ... our results show that the Akt-inhibitor ErPC3 increases radiation-induced apoptosis in prostate cancer cells The most prominent combination effects were obtained in LNCaP cells that did not show...

Ngày tải lên: 09/08/2014, 09:20

... volumes Statistical analysis Using a significance level of 5%, the Pearson s correlation test (SPSS 16.0, SPSS, Cary, NC) assessed whether correlations between variables were significant Results ... ANNs are inspired by the biological nervous system and consist of interconnected processes utilizing parallel computations, analogous to the biological neurons being the brain s processing units ... consists of many identical nodes, or neurons, mainly consisting of nonlinear activation functions The nodes are interconnected by weights, representing the interneuron synapses in the brain Further,...

Ngày tải lên: 09/08/2014, 09:20

... Dickinson LSRII instrument (BD Biosciences) We included single-stain controls for further offline compensation Measurement and subsequent analysis was performed using FACSDiva (BD Biosciences) ... patients and treatment related factors, Pearson s correlation coefficients were calculated For statistical analysis Statsoft s STATISTICA version and SPSS Statistics version 18 were used All tests ... surrounding healthy tissue 3DCRT should increase the rate of tumor control, while also decreasing side effects In spite of this focus, a higher dose to the prostate implies that the surrounding...

Ngày tải lên: 09/08/2014, 09:20

... Saitoh JI, Takahashi T, Mitsuhashi N: Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival ... cells were harvested and subjected to protein extraction The shown results represent one of three assays In controls incubated in FCS containing media and in controls incubated in media with FCS-withdrawal ... use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein...

Ngày tải lên: 09/08/2014, 09:22

... obtained To estimate the risk for seminal vesicle (SV) invasion, the Roach formula [18] was calculated for each patient If the risk for SV invasion was > 15%, as it was the case in 21 patients, ... was included in the 95% isodose and the maximum dose was limited to 105% To limit the dose to the organs at risk (OAR), a template was used with a set of dose constraints Daily treatment was intended ... was defined as complications occurring three months after the end of treatment Late toxicity at last follow-up visit was determined and was termed last late toxicity This was done to assess whether...

Ngày tải lên: 09/08/2014, 09:22

... using JMP v7.0 (SAS Institute, Cary, NC, USA) Tests for sub-groups were performed using the paired t-Test Results Figure Basic definitions of the different error components Basic definitions ... bladder distension and errors of prostate position [6] Nevertheless, we assume that image-guidance is crucial and improves the clinical outcome An assessment of patient position is based on skeletal ... analysis is standardized so that the interobserver variability is low Therefore implanted markers and EPID based methods are used for targeting in radiotherapy of prostate cancer with increasing...

Ngày tải lên: 09/08/2014, 09:22

... trends was examined using repeated measure analysis Time was used as a continuous variable in the model and t-test was used to assess statistical significance The results are presented in Table ... In a study of 16 observers of different professional disciplines (radiation oncologists, radiation therapists and physicists) using images of various anatomical sites and a 5point scale to assess ... the individuals independently performing the measures Lewis et al [9] investigated variability among observers in assessing patient movement during pelvic EBRT These authors demonstrated that interobservers...

Ngày tải lên: 09/08/2014, 10:21

... Thus, somatostatin can induce apoptosis by p53-dependent and -independent mechanisms SSTR2 induces apoptosis in a tyrosine phosphatase SHP-1-dependent manner Currently, several somatostatin analogues ... that somatostatin up-regulated the expression of SSTR1-5, and that apoptosis was activated mainly via the induced expressions of SSTR2 and SSTR3 The effects of somatostatin on the prostate cancer ... for symptomatic treatment of hormone secreting neuroendocrine tumours It has higher affinity for SSTR2 and shows significant anti-neoplastic actions in tumours expressing SSTR2 [38] It remains...

Ngày tải lên: 10/08/2014, 10:20

... immune responses A preventive or inhibitory activity of PDF on carcinogenesis and metastasis has also been demonstrated in mice [12], suggesting its antitumor activity Moreover, a chemosensitizing ... using a scan densitometer (Silk Scientific, Oregon, UT) Statistical analysis All data are presented as the mean ± SD (standard deviation), and statistical differences between groups were assessed ... addition, particularly in clinical CaP cases, IFN therapy has several drawbacks such as high cost and repeated administration [6] These disadvantages thus limit its use in clinical practice, and...

Ngày tải lên: 10/08/2014, 22:20

... validation of previous and future studies Materials and methods Prostate tissues and plasma samples from patients Prostate tissues and plasma samples were obtained from Mayo Clinic Biospecimen Core ... study using XMRV-infected rhesus macaques and sensitive ELISA detection assays showed that infected animals rapidly develop antibodies against XMRV proteins, including gp70 (Env), p15E (transmembrane), ... RNASEL R462Q in our clinical samples (Table 5) Phylogenetic analyses of MLV-like sequences in prostate tissue DNA XMRV has been PCR amplified from prostate cancer samples in a number of studies...

Ngày tải lên: 13/08/2014, 01:20

... exhibiting dasatinib-responsive expression patterns were examined using a previously published prostate tumor data set consisting of 52 tumor samples [16] As shown in Figure 4b, nearly 44% (23/52, sample ... of these 174 genes on the 16 cell lines was visualized by cluster analysis As shown in Figure 1c, these 16 cell lines were separated into two major groups (the dasatinib-sensitive cell lines are ... TM, Slawin KM: Association of the circulating levels of the urokinase system of plasminogen activation with the presence of prostate cancer and invasion, progression, and metastasis J Clin Oncol...

Ngày tải lên: 14/08/2014, 08:20