... that the salt optima of the enzymes are fine-tuned to match the salinity of their environment The outer membrane and cell wall of Gram-negative bacteria not restrict passage of ions, and the periplasmic ... energy of activation Results from the thermodynamic calculations (Table 2) reveal that there is a slight difference in the free energy of activation between the two enzymes originating from both ... enzyme must sacrifice some of its stability It has previously been suggested that the lower thermal stability of cold-adapted enzymes is simply a consequence of the lack of selective pressure for...
Ngày tải lên: 19/02/2014, 05:20
... sequence of the a-chain of abrin-a, a toxic protein from the seeds of Abrus precatorius Agric Biol Chem 52, 10951097 40 Kim Y & Robertus JD (1992) Analysis of several key active site residues of ricin ... at 60 C) with 20 lL of m aniline-acetic (pH 4.5) or 20 lL of H2O for nonaniline-treated controls The reactions were stopped by the addition of 0.1 vol of NH4OAc and 2.5 vol of 100% ethanol and ... were calculated from deconvolution of the CD spectra using the program selcon [29] employing a database of 42 proteins Biological activity in vivo of the rPAB The biological activity of the recombinant...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... Structure–function studies of E cloacae IDPC (Eur J Biochem 270) 2323 Fig Scheme of the postulated biosynthesis pathway of indole-3-acetate from L-tryptophan in E cloacae including the keto-enol tautomerism of ... integral of with respect to the indole protons is consistent with the occurrence of the enol form of indolepyruvate (Fig 1) In the course of the establishment of the equilibrium % 85% of the enol ... of the cofactors The enzyme is destabilized at low ionic strength The stability of EcIPDC in aqueous solutions is higher than that of other PDCs The rate constant of inactivation of PDC from Pisum...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot
... a ˚ 3-A dissociation of subunits interacting by a1 The base of dTTP is inserted between the a1-helix of two subunits and is stacked between the rings of Phe18 and Phe34 from the adjacent subunit ... case of Ba-TK, several hydrogen bonds form between sidechain atoms and main-chain atoms of the neighboring helices: NH1 of Arg27 is hydrogen-bonded to mainchain oxygen of Ser19, and NH1 of Arg31 ... lack of substrate in Bc-TK leaves the lasso-loop flexible The open conformation of the lasso-domain in Bc-TK resembles the lasso-loop of Ca-TK [17], where part of the loop is missing because of...
Ngày tải lên: 23/03/2014, 09:21
Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx
... rescue studies remains elusive We plotted log(krescue k0) of R507A and K512A against the pKa of the rescue agent taking data from Table 2, assuming that all of the listed derivatives of guanidine ... bridge with Lys540 Results from 31P-NMR studies revealed an indirect interaction of Glu638 with the 5Â-phosphate group of the cofactor [41] and suggested participation of the glutamate in establishing ... concentration of phosphate was varied at a constant concentration of a,a-trehalose (400 mm) in the absence or presence of different constant concentrations of vanadate Enzymatic rates of hydrolysis of a,a-trehalose...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo hóa học: " Decoding subtle forearm flexions using fractal features of surface electromyogram from single and multiple sensors" docx
... consisting of FD and MFL of single channel sEMG are indicators of relative strength of contraction of Figure Estimated shape of MUAPs at the surface when they originate from different distances from ... significant variations of FD of a muscle with small changes of muscle contraction Page of 10 A new feature, the maximum fractal length (MFL) of the signal, as a measure of the muscle activation ... separation of the different classes This provides a qualitative analysis of the data Results Figure is the sample representation of the MFL of multiple channels for different wrist flexions From this...
Ngày tải lên: 19/06/2014, 08:20
báo cáo hóa học: " Multiple myeloma and farming. A systematic review of 30 years of research. Where next?" pdf
... farming and Multiple Myeloma from published case control studies Individual and pooled OR for farming and Multiple Myeloma from published case control studies Plotting the standard error of the OR ... analysis of female farmers reported a RR of 1.38; 95% CI 1.27 to 1.43 The sub group of thirteen cohort studies had a pooled RR of 1.13; 95% CI 1.09 to 1.17 However, they did not include an analysis of ... rubber We present a meta analysis of case control studies of occupation and MM This first article reports our analyses of those studies reporting the effect of farming and other agricultural...
Ngày tải lên: 20/06/2014, 00:20
Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
... at multiple testing sites Conclusion Figure subtypes RT-PCR amplification of NA gene fluids One-stepusing animal samples from allantoic from all NA One-step RT-PCR amplification of NA gene from ... of 1,101 fulllength NA sequences of the subtypes, of a range of host species and from different geographical locations were retrieved and aligned using Biological Sequence Alignment editor software ... extracts from clinical NPA specimens were tested The clinical specimens were from reported cases of viral respiratory disease from Sydney in the period of 2003–2004 Viral RNA was extracted from these...
Ngày tải lên: 20/06/2014, 01:20
Báo cáo vật lý: "Preliminary Studies for Production of Fatty Acids from Hydrolysis of Cooking Palm Oil Using C. rugosa Lipase" pot
... withdrawn from the oil at every 30 from each flask 2.3 Determination of Degree of Hydrolysis (Conversion) and Rate of Hydrolysis The degree of hydrolysis was determined by titration of the oil ... Hydrolysis Profile Figure shows a typical hydrolysis profile of palm oil at temperature of 45ºC for an initial palm oil concentration of 0.1 g palm oil/ml iso-octane at agitation speed of 200 rpm ... indicator The degree of hydrolysis, X is calculated as below: X,% = (ml NaOH used) (molarity of NaOH) (average molecular weight of fatty acid) 10 (weight of sample) (1) The rate of hydrolysis, r...
Ngày tải lên: 07/08/2014, 14:20
Báo cáo khoa học: "Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog" pps
... amplification Preparation of canine blood cell A normal healthy adult dog served as a blood donor PBMCs were separated from approximately 10 ml of venous blood supplemented with 1.14 ml of CPDA as anticoagulant ... stranded cDNA was made from total RNA of LPSstimulated PBMCs and amplified using the polymerase chain reaction with il-6S and il-6R primers derived from the sequences of canine IL-6 mRNA Agarose ... region of canine IL-3 and IL-6 from the blood cells of a dog by PCR The canine IL3 open reading frame begins with an ATG start codon and ends at a TGA stop codon A computer-assisted alignment of...
Ngày tải lên: 07/08/2014, 15:20
Báo cáo khoa học: "From the “Annales de l’École Nationale des Eaux et Forêts” to the “Annals of Forest Science”, eighty years of forestry science publications in France" docx
... (1995) 521–680 Eighty years of forestry science publications Figure Annual evolution of the percentage of articles from foreign authors an the percentage of articles in English from 1923 to 2001 ... shame, in terms of speed of diffusion of scientific information that the forest managers have turned away from this type of publication The problem of simplifying the results of scientific research ... 393–622 The referees are of various nationalities and in 2001, 57% of the total number of referees were foreigners During this profound evolution, apart from the nationality of the authors, the change...
Ngày tải lên: 08/08/2014, 14:21
báo cáo khoa học: " Rethinking the conceptual terrain of AIDS scholarship: lessons from comparing 27 years of AIDS and climate change research" ppt
... to elucidate why the task of understanding the root causes of the uneven impacts of AIDS (and of climate change) has not been (or has failed to remain) at the forefront of dominant research or ... MB acknowledges the support of Carleton University, and AW acknowledges the support of the University of KwaZulu-Natal, and the DFID Addressing the Balance of Burden of AIDS Research Partners Consortium ... This led to a re-medicalization of HIV/AIDS and increasing international pledging of resources (see Figure 1) The development of generic drugs meant the price of medicine had fallen to about...
Ngày tải lên: 11/08/2014, 14:21
Báo cáo khoa học: "A Toxicity of polymyxins: a systematic review of the evidence from old and recent studies" ppsx
... effect of the use of polymyxins Occurrence of haematuria, proteinuria, cylindruria, or oliguria may also be associated with the administration of polymyxins In addition, Page of 13 Old studies (from ... with the use of polymyxins, mainly of colistin, revealed a high incidence of nephrotoxicity The majority of the studies in the older literature referred to intramuscular administration of colistimethate ... assessment of polymyxin-induced toxicity Dosage adjustment of polymyxins in the presence of impaired renal function and prompt discontinuation of polymyxins after development of early signs of their...
Ngày tải lên: 12/08/2014, 23:21
Báo cáo y học: "Data from necropsy studies and in vitro tissue studies lead to a model for allometric scaling of basal metabolic rate" pot
... approximates the sum of terms with similar values of βi This midpoint is estimated as the midpoint of the values of ki plus the midpoint of the values of bi because values of ki are not available ... scaling of the brain and the epithelial tissues of the skin and gastrointestinal tract, the midpoint of the values of bi is 0.71 The midpoint of the values of ki is -0.14, the midpoint of the ... avMm0.86 At this value of M, the epithelium- Second-order polynomial regression of log10(BMR) yields a coefficient of [log10(M)]2 of 0.038 with 95% CI of 0.026 - 0.049 from the data of Heusner and yields...
Ngày tải lên: 13/08/2014, 23:20
constructing shared understanding - a grounded theory exploration of team case formulation from multiple perspectives
... Assessment of risk of bias in included studies All studies selected were assessed for risk of bias using the Cochrane Collaboration Risk of Bias tool (Higgins et al., 2011a) to ascertain the validity of ... majority of results of between-group comparisons were non-significant More importantly, risk of bias within individual studies was high Only three studies were judged low in terms of risk of bias, ... characteristics of included studies (including participants, interventions, methodology and risk of bias) The outcomes of the studies were summarized by grouping them under areas of outcome and...
Ngày tải lên: 22/12/2014, 21:57
Estimating the attributable mortality of ventilatorassociated pneumonia from randomized prevention studies.
... 1-RR) of VAP and mortality and their corresponding 95% confidence intervals by pooling all studies, studies with a statistically significant reduction of VAP, studies with comparable RRR of VAP ... low levels of heterogeneity (Table 3) Statistically significant RRRs of mortality were also not obtained from the pooled analyses of studies reporting statistically significant reductions of VAP (RRR, ... apparent outliers of 26% for studies being “highly effective” in preventing VAP and studies with a high incidence of VAP DISCUSSION Based on the data of 58 randomized comparisons of preventive measures...
Ngày tải lên: 31/07/2015, 18:38
Automated combination of probabilistic graphic models from multiple knowledge sources
... accomplishment of this thesis Their enthusiasm and kindness will forever be remembered Professor Peter Haddawy from Asian Institute of Technology (Thailand) and Professor Marek Druzdzel from University of ... represents the instantiation of the variable Xi and πi represents the instantiation of the parents of Xi Any JPD of a set of random variables can be dened in terms of a set of smaller Conditional ... of the advantages of MSBN is good at supporting multi-agent systems We can see an example of MSBN from Figure 2.1, from which we can see such architecture is good at providing communication of...
Ngày tải lên: 30/09/2015, 14:24
Structure and mechanism studies of sugar epimerase from dictyoglomus turgidum
... School of Konkuk University TABLE OF CONTENTS List of Tables v List of Figures vi Abstract ix Chapter Structure and mechanism studies of sugar epimerase from ... Mechanism Studies of Sugar Epimerase from Dictyoglomus turgidum Pham, Tan Viet Department of Advanced Technology Fusion Graduate School of Konkuk University Cellobiose 2-epimerase from Dictyoglomus ... Structure and Mechanism Studies of Sugar Epimerase fromDictyoglomus turgidum A Dissertationsubmitted to the Department of Advanced Technology Fusion and the Graduate School of Konkuk University...
Ngày tải lên: 20/05/2016, 21:08
Removal of congo red from wastewater by adsorption onto waste red mud
... Fe(III)/Cr(III) Namasivayam of dairy (11) and coworkers from employed solutions of using containing congo fly ash and coal(g) et al (12) have of nickel of this a textile such of chlorophenol study ... initial with stages lapse of of time 90 uptake for all the dye of dye decreased concentrations curves monolayer increased from were single, smooth coverage of on dye and outer of adsorbent 20 40 60 ... remove prepared with for from the adsorbent 50 100 mL 50 i.e The any Then of The out mL of as dye for an agitation discarded to carried with 250 mg of adsorbent solution washed studies 100 min, dye...
Ngày tải lên: 23/09/2012, 14:47
Báo cáo y học: "Antimicrobial Susceptibilities of Brucella Isolates from Various Clinical Speciemens"
... hospital of the Medical Faculty of Van Yuzuncu Yil University (Van, Turkey) Identification methods: Identification of species was made on the basis of the requirement of CO2 for growth, production of ... administration of TIG, the conservation of TIG because of promising results of its use in the treatment of multiresistant bacterial infections, and overall cost were considered as limitations of such ... the results of MICs of TIG The MIC50 and MIC90 values of relevant antibiotics are shown in Table http://www.medsci.org Int J Med Sci 2011, 200 Table MIC range, MIC50 and MIC90 values of antimicrobial...
Ngày tải lên: 25/10/2012, 11:10