xenobiotic metabolizing human cells as tools for pharmacological and toxicological research

... Heinrich ganglia neurons and bovine chromaffin cells and assumed to Betz for his support and for critical reading of the manuscript be selective for a3b2 and a3b4 interfaces and unable to inhibit ... oocyte-expressed human nAChR subunit combinabrain [64] and has proven to be a powerful radioligand in tions and seems to have a similar selectivity and activity as numerous binding and autoradiography ... addiction and neurological disorders such as Parkinson’s and Alzheimer’s disease For the nonselective nAChR agonist nicotine, analgesic, anxiolytic and cytoprotective properties are seen, as well as...

Ngày tải lên: 19/02/2014, 12:20

... OH, USA) (1 U for XmnI, 12 U for DraIII, 2.5 U for XbaI) and incubating for h at 37 °C Phosphatase was inactivated for 20 at 65 °C DNA was then ethanol precipitated and resuspended in Tris 10 mm ... complex formation was observed using both the pyrimidine and purine motif triple helices In particular, the influence of heating conditions and of the length of the stem has been studied as well as ... specificity and the sensitivity of this approach for DNA detection Fig Sequence of targets and TFOs used in this study The central part of TG and TC TFOs as well as the target sequences inserted in plasmids...

Ngày tải lên: 20/02/2014, 03:20

... plasma cells was noted under the condition of lupus Jacobi and colleagues evaluated the correlation of CD27high plasma cells and disease activity in lupus patients [51] Patients with high disease ... significantly increased frequency of CD19+/CD27high plasma cells and had a predictive value for disease activity that was greater than traditional humoral/clinical measurements (for example, anti-dsDNA, ... nephritis was inversely associated with anti-La (SS-B) and associated with anti-dsDNA It has been repeatedly shown that anti-dsDNA, single anti-Ro antibodies as well as Sm antibodies [10] are associated...

Ngày tải lên: 09/08/2014, 14:22

... methods for ODE’s, http://www.eng.mu.edu/corlissg/Pubs/03Lyngby/Slides/ [2] N S Nedialkov, Interval tools for ODEs and DAEs, http://www.cas.mcmaster.ca/∼ nedialk/PAPERS/intvtools/intvtools.pdf ... methods for classes of problems may be a feasible approach • An efficient method for stiff problems is needed • An “interval version“ of DETEST, a test set for assessing approximate solvers for IVP ... http://valencia-ivp.com/ [7] VNODE-LP, http://www.cas.mcmaster.ca/∼ nedialk/vnodelp/ [8] DAETS • Demo version at http://www.cas.mcmaster.ca/∼ nedialk/daets/ • Academic and commercial versions at Flintbox http://www.flintbox.com...

Ngày tải lên: 12/01/2014, 21:46

... prostanoids and other mediators as well as adhesion molecules such as intercellular adhesion molecule (ICAM-1) [15] In this context, ICAM-1 is particularly important in inflammatory diseases such as asthma, ... binding and transcriptional activity in A549 cells [19] Both TNFa and IL-1b produced a robust increase in reporter activity, measured h poststimulation as described previously [21] In each case, ... luciferase levels (Fig 4B) However decreased luciferase activity was observed with 10 lM SB203850 and this appeared to reach a plateau by 100 lM With PD098059 and UO126 a variable effect was observed...

Ngày tải lên: 07/03/2014, 15:20

... and the Inertial Spectrum of Turbulence 4.4 Random Measures and Random Fourier Transforms 4.5 Prediction for Stationary Stochastic Processes 4.6 Data Assimilation 4.7 Exercises 4.8 Bibliography ... view two random variables η1 and η2 as a single vectorvalued random variable η = (η1 , η2 ) = η(ω) for ω ∈ Ω We say that η is measurable if the event η ∈ S with S ⊂ R2 is measurable for a suitable ... slightly different form Let the random variables ηi for i = 1, 2, be independent and have each the pdf f , with mean and variance as above, and construct the following sequence of random variables:...

Ngày tải lên: 23/03/2014, 23:20

... cloning and embryonic stem cell research under the Research Involving Human Embryos Act and the ISASI R.M., KNOPPERS B.M Prohibition of Human Cloning Act These two acts limit research on human ... other than by the fertilization of a human egg by a human sperm and the creation of human embryos for research purposes Therefore, creating a human embryo for purposes other than achieving pregnancy ... produce human fetuses based on ethics and safety With respect to research using human embryonic stem cell lines for the cloning of human tissues, the academy is of the opinion that human cells, ...

Ngày tải lên: 28/03/2014, 16:20

... specialists and a data manager Inter-rater reliability assessments formed part of this training The intra-class correlation value for interrater reliability of the lay research assistants was 0.98 ... screening for current MDD was 22 This achieved a sensitivity of 73% and PPV of 76% (Figure 3A) For the AUDIT, the optimum cut-off score for screening AUDs was 24 for women (sensitivity of 60% and PPV ... AUD cases from non-cases respectively (AUROC for CES-D = 0.78, and for AUDIT = 0.98 for women and 0.75 for men respectively), indicating that these were accurate screening tools The difference...

Ngày tải lên: 11/08/2014, 15:22

... expression has been demonstrated in alveolar and bronchial epithelial and vascular endothelial cells [23,31,32] Sha et al demonstrated that ECs express mRNA for all TLR and that several known TLR ligands ... activation of NF-B and MAPK, whereas TRIF and TRAM are critical for late activation of NF-B as well as the activation of IRF-3 [9] There is no consensus about the expression and role of CD14 in ... -18R signalling A role for IL-1 and IL-18 pathways in this response was excluded, as bronchoconstriction, inflammation, and protein leak were normal in IL-1R1 and caspase-1 (ICE)-deficient mice Furthermore,...

Ngày tải lên: 12/08/2014, 14:20

... solutions in two different cases, and leave other cases as exercises for your fun and exploration © 2001 by CRC Press LLC Case Here, a = –0.99, and we consider the cases b = and b = 0.98 The starting ... the functional form of the homogeneous and particular solutions are the same, use the same functional form for the solutions as in the case of multiple roots for the characteristic polynomial.) ... usually assumed to be zeros On the other hand, in the difference equation formulation of this class of problems, the present output y(k) is expressed as a linear combination of the present and m...

Ngày tải lên: 13/08/2014, 02:21

... parameters b Holding the graph for the case R = 100Ω, sketch L1 and L2 again for R = 50Ω and R = 500Ω How the values of the voltage and the current change as R increases; and decreases? c Determine the ... required to describe the random variable X In-Class Exercises Pb 3.20 In each of the following cases, find the entropy: a N = 32 and p( xi ) = for all i 32 1 1 1 1  b N = and p =  , , , , , , , ... log( A) for x ≤ / A y=± + log( A x ) + log( A) for ≤ x ≤1 A Here, the + sign applies when x is positive and the – sign when x is negative x = vi/V and y = vo/V, where vi and vo are the input and...

Ngày tải lên: 13/08/2014, 02:21

... iterative equations for D(k) and D2(k), as given respectively by Eqs (4.16) and (4.30), and with the initial conditions for the function and its derivative The first elements for the y, D, and D2 arrays ... title('Modulated Signal') Dphase(1)=0; for k=1:p-1 Dphase(k+1)=(2/dt)*(phase(k+1)-phase(k))- Dphase(k); end md=Dphase/(2*pi*kf); subplot(2,2,4) plot(t,md) title('Reconstructed Message') As can be observed ... plot(t,y,t,u,' ') The dashed curve is the temporal profile of the source term In-Class Exercise Pb 4.38 Plot the amplitude of y and its dephasing from u as function of a for large t, for 0.1 < a < Application...

Ngày tải lên: 13/08/2014, 02:21

... analytically MATLAB has a built-in command that finds all the roots (real and complex) for any polynomial equation As previously noted, the MATLAB command for finding the polynomial roots is roots: ... choose your mirrors for maximum contrast? g Plot the contrast function defined as C = © 2001 by CRC Press LLC h For ω = ω0, plot the variation of the transmission coefficient as function of θi i ... knowing the roots, we can reassemble the polynomial The command is poly poly(r) In-Class Exercise Pb 5.14 Find the roots of the polynomial p = [1 pute their sum and product 3], and com- Pb 5.15 Consider...

Ngày tải lên: 13/08/2014, 02:21

... sum phasor tude and phase for each have the functional forms An = © 2001 by CRC Press LLC Solution: Edit and execute the following script M-file: N=10; n=1:N; amplituden=1./n; phasen=n.^2; phasorn=amplituden.*exp(j.*phasen); ... phasorn=amplituden.*exp(j.*phasen); phasortot=sum(phasorn); amplitudetot=abs(phasortot) phasetot=angle(phasortot) In-Class Exercises Pb 6.36 Could you have estimated the answer to Example 6.8? Justify your reasoning ... values for the lower and upper cutoff frequencies and the sampling time: f1 = 200 Hz; f2 = 400 Hz; τ = 10–5 s find f0 and plot the gain curve as function of the normalized frequency for the bandpass...

Ngày tải lên: 13/08/2014, 02:21

... non-zero and such that the location of this non-zero element is different for each of these basis vectors This basis is not unique For example, in 4-D space, the canonical four-unit orthonormal basis ... vectors u, v , and w is found through the det([u;v;w]) command Make sure that the vectors defined as arguments of these functions are defined as 3-D vectors, so that the commands work and the results ... the basis vectors is written as: m n = δ m ,n (7.26) where the basis vectors are referred to by their indices, and where δm,n is the Kroenecker delta, equal to when its indices are equal, and...

Ngày tải lên: 13/08/2014, 02:21

... between performing an ac steady-state analysis of a circuit with purely resistive elements, as was done in Subsection 8.7.1, and performing the analysis for a circuit that includes capacitors and inductors, ... Circuit of Pb 8.6 Assuming that R1 = R2 = Ω, RL = Ω, C = 0.25 F, and L = H: a Write the equations for the phasors of the voltages and currents b Form the matrix representation for the equations ... matrices, as these form the tools for the matrix formulation of ray optics and Gaussian optics, which are two of the major sub-fields of photonics engineering Example 8.16 Find the eigenvalues and eigenvectors...

Ngày tải lên: 13/08/2014, 02:21

... employed in the handling and manipulation of digital images Let us explore and observe the structure of a matrix subjected to the above elementary trasformations For this purpose, execute and observe ... Currently more and more images are being stored or transmitted in digital form What does this mean? To simplify the discussion, consider a black and white image and assume that it has a square boundary ... efficient method for building a scrambling matrix In-Class Exercises Assume for simplicity that the 2-D array size is (10×10), and that the scrambling matrix is chosen such that each row has one element...

Ngày tải lên: 13/08/2014, 02:21

... noting that the events E and O (B and C) are disjoint and that their union spanned the set S Therefore, the probabilities for events O and C could have been deduced, as well, through the relations: ... monotonically increasing for b < 0, and monotonically decreasing for b > If b1 > b2 > 0, then everywhere on the positive x-axis, y1(x) < y2(x) The exponential function has no roots For b > 0, the ... Solution: Let O be the event that was received, and Z be the event that was received If H1 is the hypothesis that was received and H0 is the hypothesis that was received, then from the statement...

Ngày tải lên: 13/08/2014, 02:21

... software tools that use a relational database for information storage, and an XML-based file format used for transmission of this information and storage outside of databases The OME XML file format ... OME XML file format is not raw performance, but interoperability above all else, using widely accepted standards and practices for information exchange As the OME XML file format has gained acceptance, ... including databases, editing tools, and parsing libraries Finally, and perhaps most important, XML is a plain-text format As a last resort, it can be opened in any text editor and the information...

Ngày tải lên: 14/08/2014, 14:21

... ClearCanvas image-based measurement tools and the tools available with the AIM plug-in VITA parsed these annotation files using its inbuilt XML parser and generated visual reports for each exam based ... downloaded and checked in another workstation to ascertain that all the information was properly rendered and saved Figure 2.4 shows a snapshot of VITA A ClearCanvas measurement tool was 19 CHAPTER ... find sc and tc for L1 and L2 If

Ngày tải lên: 09/09/2015, 11:23