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... accessed here: http://www.biomedcentral.com/1471-244X/9/25/pre pub Publish with Bio Med Central and every scientist can read your work free of charge "BioMed Central will be the most significant ... acceptance cited in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page 12 of 12 ... was not demonstrated statistically because our study was not sufficiently powered Using less restrictive ttests to examine group differences at specific points of time, we demonstrated a statistically...

Ngày tải lên: 11/08/2014, 17:20

... a lot of other people, we managed to survive the recession a Contrary to b In contrast to c On the contrary with d In contrast with > b 212 If you me, just say so a disagree with b differ ... necessary forms and for the job a appealed b asked c requested d applied > d 170 She is traveling to work by train today because her car is being a stopped b broken c serviced d rented >c 171 ... of b takes track of c falls in love with d gets in touch with > c 227 Do you the long distance telephone calls which you make in your office each month? a pay attention to b keep track of c...

Ngày tải lên: 05/11/2012, 09:18

... recreation, perhaps because the Sunday is "the day of rest." Holiday is also a common time for recreation Traditionally, music and dance serve as recreation in many cultures Playing sports, hobbies, games ... printing B guest C nursing D private The Americans may be much more concerned physical attractiveness than the Indians and the Chinese when choosing a wife or a husband A of B on C in D...

Ngày tải lên: 02/07/2013, 01:25

... IL-1b, TNF-a and IL-10 secretion by MSCs The MSCs-CM was concentrated 20 · by ultrafiltration using 10 kDa molecular mass cut-off ultrafiltration membranes (Millipore) following the manufacturer’s ... induced significant TNF-a transcription, the translation of TNF-a remained unchanged even when TNF-a transcription was inhibited by MG132 or BAY 11-7082 The exact reason for the translational repression ... SD-induced IL-1b and TNF-a transcription, also abolished pro-IL-1b translational upregulation (Fig 4B,C) but failed to affect TNF-a translation A B C Fig IL-1b and TNF-a transcriptional induction...

Ngày tải lên: 18/02/2014, 04:20

... determined using the articial substrate 4MU-(GlcNAc)3 (Sigma) The maximum substrate concentration used had to be limited to about twice Km because of the occurrence of substrate inhibition (which is ... conceivable that the real natural substrates of the CBP proteins differ from the substrates used here and in other studies There may exist composite natural chitinous substrates that are more susceptible ... solid substrates by boiling in SDS-PAGE sample buffer after the substrates had been washed to remove nonspecically bound protein Note that the samples in (B) are approximately sixfold concentrated...

Ngày tải lên: 18/02/2014, 08:20

... primers were used: upper strand primer, 5¢-CCTTCACCATCCT CTCTTTCCCCCAC-3¢, corresponding to nucleotides 262– 237 of the reverse strand of the b3Gal-T5 coding sequence; lower strand primer, 5¢-CAGGTTCAGGGGGAGGTGT ... chose BxPC3 cells for transfection as they express low levels of the transcript (0.2 fgÆpg)1 b-actin) but still well detectable amounts of b1,3Gal-T activity (16.0 nmol transferred GalÆmg protein)1Æh)1) ... pancreatic adenocarcinoma cell line BxPC3 (A) Total RNA was extracted from BxPC3 cells and T5AS clone, reverse transcribed, and the first-strand cDNA obtained was diluted : 20, v/v, with water PCR...

Ngày tải lên: 19/02/2014, 12:20

... concentration of lm for a 10 day treatment, replaced with fresh medium every days Transient transfections and luciferase assay Cells were plated days before transfection We performed transient transfection ... demonstrated to bind to ERRa and the complex ERRa–PGC1a [24,25] No effect on reporter activity was observed after transfection with PRC alone Forced overexpression of ERRa, without PRC transfection, ... PRC–ERRa complex activates transcription directly through a consensus estrogen response element (ERE) To determine whether PRC can function as a coactivator of ERRa, transient transfections into RO...

Ngày tải lên: 06/03/2014, 09:22

... Mutant strain of 8325-4 defective in protein A Strain 8325-4 with repaired defect in rsbU Mutant strain of SH1000 defective in protein A Mutant strain of SH1000 defective in FnBPs Mutant strain ... Rabbit virulent strain expressing FnBPs Mutant strain of P1 defective in protein A Mutant strain of P1 defective in FnBPs Mutant strain of P1 defective in FnBPs and protein A Mutant strain of P1 defective ... constructs were transduced from strain 4500 Source or reference 40 41 42 41 43 This work This work This work 44 41 23 This work This work This work 45 41 Stratagene Novagen RN4220 to strains P1 fnbA...

Ngày tải lên: 06/03/2014, 22:21

... limited In vitro, Cu(I) titrations of isolated MT-2 and its separate domains demonstrated that up to six Cu(I) ions can bind to each domain [26] In another extensive titration study, it was postulated ... equivalent Cu(I) was added The spectra of the samples containing three, four and five equivalents A B 20 10 -10 Zn4- -MT Fig CD spectra of Zn4aMT (A) and Zn3bMT (B) along the titration with Cu(I) Samples ... of titration One possible explanation for the disappearance of NOESY signals at high Cu(I) concentration might be that two or more Cu(I) 2352 600 / nm 650 700 Fig Luminescence emission spectra...

Ngày tải lên: 07/03/2014, 09:20

... (left trace) and after 20 exposure to 0.5 lM (middle trace) and lM a1-PTH (right trace) (C) Average of 30 sequential MEPPs recorded before (left trace) and after 20 exposure to lM PIN-a (right trace) ... of extracellular Ca2+ concentration on the effect of a1-PTH (B and C) Note that in (A) only a1-PTH produces membrane depolarization, and in (B) and (C) increasing extracellular Ca2+ concentration ... neuromuscular transmission and muscle contraction evoked by nerve stimulation In contrast, no changes were detected with PIN-a, except for hyperpolarization of the muscle membrane when high concentrations...

Ngày tải lên: 07/03/2014, 12:20

... parameters measured at different proton concentrations are given in Table In this set of experiments, the HbA concentration is 100 lm in heme At such a concentration no more than 10%, by weight, of ... concentration on the rates of O2 binding to the a and b subunits within liganded tetramer As evident from the experiment at pH 8.5 and the previous one at pH 7.4 [31], the effect of NaCl concentration ... the chloride concentration 6114 increases at pH 8.5, complete dissociation of tetrameric hemoglobin to dimer without a detectable change in the O2-binding properties of the tetramer may be inferred...

Ngày tải lên: 16/03/2014, 14:20

... exactly as calculated from the amino acid sequence The purified protein was concentrated to 60 mgÆmL)1 by ultrafiltration using kDa JumbosepTM membrane (Pall Corporation, MI) Ó FEBS 2004 4116 W.-C ... are shaded in red, green and blue for acidic, neutral and basic polar residues, and in yellow for prolines and glycines In (C) the polypeptide tracings of two SUMO-2 models from type I (12–89) ... regions of 28–43 and 71–83, that correspond to the strand b2, the N terminus of the helix a1, the helix a2, and the connecting loop to the strand b5 (Fig 3A,D) Such a large difference between...

Ngày tải lên: 16/03/2014, 18:20

... that were lm thick Transfection of EA.hy.926 cells Cells were transfected using the calcium phosphate–DNA coprecipitation method as described in Jordan et al [55] For transient transfections, cells ... dependent on an influx of extracellular calcium, consistent with previous work on HUVECs [32], which demonstrated that agonistevoked prostacyclin production is dependent on extracellular free [Ca2+] ... grown overnight One hour prior to transfection, the cells were fed with fresh medium Cells were transfected with lg plasmid DNA per coverslip Six hours post-transfection, the DNA–calcium phosphate...

Ngày tải lên: 16/03/2014, 18:20

... fragmented by nebulization • The DNA strand’s ends are made blunt with appropriate enzymes • “A” and “B” adapters are ligated to the blunt ends using DNA ligase • The strands are denatured using sodium ... • The B adapter contains a 5’ biotin tag used for mobilization • The beads are magnetized and attract the biotin in the B adaptors Filtering the Mess • There are four adaptor combinations that ... DNA rich beads only • A magnetic field filters all DNA rich beads from empty beads, and then extracts the biotin beads from the DNA rich beads • The DNA in the beads are denatured again using...

Ngày tải lên: 19/03/2014, 22:32

... ‘Molecular and Cell Biology’) References Spectral studies UV spectra were recorded with a Cary 50 Bio spectrophotometer Alternatively, the UV spectra were recorded online 4470 Grechkin AN (1998) ... species of galactolipids were separated by RP-HPLC using an online UV spectral record with a diode array detector Galactolipids extracted from unstressed flax leaves possessed a single predominant molecular ... leaves Total galactolipids were extracted from flax leaves, separated and purified as described in Materials and methods UV chromatograms (267 nm) of galactolipids extracted from: (A) unstressed plants,...

Ngày tải lên: 23/03/2014, 05:22

... often present in total cell extracts of yeast [45] (B) Levels of HSE2-LacZ reporter gene activity in strain PSY145* with wild-type Hsf1p [22] (hatched bars) or strain PSY145*HSF(1–583) with a ... constructed slt2D mutant versions of strains PP30[hHsp90a] and PP30[hHsp90b] (see Experimental procedures) These strains (PP30[hHsp90a]slt2D and PP30[hHsp90b]slt2D) were then transformed with either a control ... probed with anti-(Achlya Hsp90) and anti-tetra-His sera Control lanes (C) are the extracts from unstressed, non-ERK5-expressing cultures of the same strains Gal4p activator domain (AD)-Slt2p and...

Ngày tải lên: 23/03/2014, 07:20

... functionally exchanged with the transit peptide of pPORB and vice versa, and the transit peptides were fused to a reporter protein of mouse It was found that only the isolated transit peptide of PORA ... in the step of precursor translocation in vivo Proc Natl Acad Sci USA 93, 12026–12030 Reinbothe S, Runge S, Reinbothe C, van Cleve B & Apel K (1995) Substrate-dependent transport of the NADPH:protochlorophyllide ... low concentration or if blocked at the N-terminus [25] We show that the N-termini of PORA and PORB of barley and POR from pea are homologous, and that Pchlide cannot bind to the PORA transit peptide...

Ngày tải lên: 30/03/2014, 02:20

... catalytically active in the dephosphorylated state (phosphorylase b) at physiological AMP concentrations [16] Titrated MGP expression in hepatocytes causes inactivation of glycogen synthase and inhibition ... activity and concentration of phosphorylase-a on glycogen synthesis To test the role of the phosphorylated form of phosphorylase independently of changes in total phosphorylase concentration, we used ... glucose concentration Glycogen synthesis (A) was determined in hepatocytes from female Zucker fa ⁄ fa (filled symbols) and Fa ⁄ ? (open symbols) rats during incubation with the glucose concentrations...

Ngày tải lên: 30/03/2014, 11:20

... Tris ⁄ HCl, pH 8.0 The protein was then concentrated in a tangential flow ultrafiltration system (Pellicon 2; Millipore) and applied to an S-300 gel filtration column equilibrated in 20 mm Tris ⁄ ... recombinant cardosin A antibody (lower panel) Lane 1, whole extracts of mature pistils used as a positive control; lane 2, pistil extracts incubated with protein A–Sepharose in the absence of PLD ... the proteins were extracted in mL Tris-buffered saline (NaCl ⁄ Tris, pH 7.0) containing mm phenylmethanesulfonyl fluoride, lm pepstatin A and 200 mm octyl glucoside The extract was centrifuged...

Ngày tải lên: 30/03/2014, 11:20

... Species GenBank/EBI A transferase A transferase A transferase A (cis A/B) transferase A-likea Gal transferase Gal transferase Gal transferase Gal transferase Gal transferase Gal transferase IGb3 synthetase ... antigens of stably transfected CHO cells CHO cells previously transfected with the rat FTB cDNA encoding an a1,2fucosyltransferase were cotransfected with the rat A enzyme cDNA Stable transfectants ... detected on extracts from the control FTB transfected cells using 2¢ fucosyllactose as acceptor However, cell extracts from the double transfectants showed a high N-acetylgalactosaminyltransferase...

Ngày tải lên: 31/03/2014, 09:20