... the user interacts with the calendar_dc instance, the date information in the text fields is updated accordingly. Clicking the showToday_btn instance resets the calendar_dc instance and text field ... value of minute to convertMinute without doing anything." In the end, convertMinute has a value between 00 and 59. 7. Insert the following line after the script added in Step 6: 8. 9. var ... user's interacting with the calendar_dc instance. When this occurs, a Date object representative of today's date is placed into calendarDate. The information contained in the calendarDate...
... Chennai, IndiaPrinted on paper from sustainable resourcesPrinted in India by Replika Press Pvt. LtdFeatherstone (Working) -3556-Prelims.qxd 5/24/2007 10:39 AM Page iv2 UnderstandingMasculinitiesIntroductionThe ... argued foran understanding of masculinity that recognised dominant interpretationsand definitions of masculinity as embedded in and sustained by a range ofmale-dominated institutions such as ... Masculinity was a vital tool in the armoury of maledominance, informing the gender system while legitimising and reinforcingmale power and the institutional aspects of male power connected with...
... nevertheless I am interested in taking part in society building. I decided to get involved in local political work in the late 90:s. Even if I did not feel particularly comfortable when thinking of the ... technology, information technology as infrastructure, an interest in activities and in organization and mission, are new focuses in the new curriculum for informatics’, all included in Dahlbom’s ... Magazin for Association of Computing Machinery), 2001, p.49. BLEKINGE INSTITUTE OF TECHNOLOGY Blekinge Institute of Technology, situated on the southeast coast of Sweden, started in 1989 and in...
... short interfering RNA (siRNA) in human cells. BothHO-2 mRNA and protein are expressed in the eight human cancer cell lines examined, and HO-1 expression is detectable in five of the cell lines,including ... obtained with pGL3Basic; the normalized luciferase activity usedwas that in cells treated with siGAPDH in (A) and that in cells untreated with hemin in (B). The data are mean ± SEM from three independ-ent ... aftertreatment with hemin (Fig. 9A), whereas the hemecontent remained unchanged in HepG2 cells treated with hemin (Fig. 9B). Thus, heme may be more effi-ciently incorporated into HeLa cells than HepG2 cellsor...
... leadingto internal dissonance (Wright 2006).Toxic Interactions (TI) Theory locates the problem in theinteraction rather than the par ties involved.Conceptually the toxicinteractions occur in ... service user and gathering informationfrom what is said and from behaviour·gathering information from existing records·gathering information from other agencies·gathering information from significant ... friends·weighing up the relative accuracy and reliability of information.(Baldwin 1997; Davis et al. 1990;Social Care Institute for Excellence 2003; Trevithick 2005) In health and social care settings...
... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... the wild-typeprotein. Similar staining patterns were observed in other human cell lines, including hepatoma Hep3Bcells and fibroblast 2fTGH cells (data not shown), sug-gesting that mislocalization ... proteins wasnot cell- type-specific. Aggresomes are structuresformed in the presence of misfolded proteins and con-tain components of the protein degradation and refold-ing machinery, including...
... enterotoxin in human intestine and in ahuman intestinal cell line (Caco-2). J. Cell Physiol. 156, 138–144.20. Cohen, M.B., Hawkins, J.A. & Witte, D.P. (1998) GuanylinmRNA expression in human intestine ... earlier[31]. Cells were plated on coverslips, washed with NaCl/Piand fixed in NaCl/Picontaining 4% paraformaldehyde for20–30 min. Cells were washed and incubated with 2%bovine serum albumin and ... expression in cells wasinhibited in cycloheximide-treated cells.To determine whether inhibition of glycosylation couldprevent recovery, desensitized cells were treated with swainsonine, an inhibitor...
... FEBSVaccination with prion peptide-displayingpapillomavirus-like particles induces autoantibodiesto normal prion protein that interfere with pathologicprion protein production in infected cellsAlessandra ... before being subjected to FACS ana-lysis. After exclusion of dead cells by 7-aminoactinomycinD staining, analysis was performed using a FACScaliburflow cytometer (Becton Dickinson, Franklin Lakes, ... Mercadante AF, Zanata SM, Martins VR,Jay DG & Brentani RR (2000) Laminin-induced PC-12 cell differentiation is inhibited following laser inactiva-tion of cellular prion protein. FEBS Lett 482, 257–260.43...
... unidentified ninhydrin-positive component suggestthat the cell wall contains other polymer(s) in addition toribitol teichoic acid.The polymers present in the cell wall wereinvestigated using NMR ... modification of sialic acid, isabundant in animal tissues [4] and, presumably, plays a role in intercell interactions [5]. In the present work, we investigated cell wall polymersof yet another representative ... after preliminary disruption by sonication,and purified using 2% (w/v) SDS to avoid possiblecontamination with membrane compounds, includinglipoteichoic acids, washed several times with water,...
... the cellulose chain stiffness and contributes to load transfer along the chain, is also indicated in Fig. 2. Other crystalline forms of cellulose include cellulose II, cellulose III, and cellulose ... natural linear aminopolysaccharide of 1-4 ȕ-D-glucosamine derived from chitin by deacetylation. Chitin (linear 1-4 ȕ-N-acetyl-D-glucosamine polysaccharide) exists mainly as a structural polymer in ... end addition in the papermaking process. In the case of carboxymethyl cellulose (CMC), the latter constraint has been circumvented by modifying the fibres during pulping or bleaching, i.e. prior...
... receptor together with G proteinsincreased GTPcS binding. The cannabinoid receptor agonistWIN 55,212–2 (A) further increased GTPcS binding. This bindingwas inhibited by the cannabinoid selective ... [19] using the G proteins purified frombovine brain. The relatively small increase in GTPcSbinding to G protein on addition of the agonist WIN55,212–2 in our experiments can be explained by ... plate. After 4 days of incubation, the cellswere harvested by spinning in a centrifuge; 2 · 105cellsfrom each clone were lysed using 1% SDS in a buffer con-taining protease inhibitors and DNase....
... doxycycline-inducible protein expression by just a single transfec-tion. pEBTetD contains an Epstein–Barr virus origin of replication forstable and efficient episomal propagation in human cell lines, ... lmolÆL)1) in uptake buffer. Incubation was stoppedafter 1 min by rinsing the cells four times each with 4 mLice-cold uptake buffer. Subsequently, the cells were solubi-lized with 0.1% v ... were selected with 3 lgÆmL)1puromycin (PAA Laboratories); puromycin wasalways present in subsequent cell culture to ascertain plas-mid maintenance. To turn on protein expression, cells werecultivated...
... in nite size,including all substrings appearing in the large cor-pus in the target domain. In experiments, we esti-mated LMs from a relatively small annotated cor-pus in the general domain ... Phoneme-to -Text Transcription with an In nite VocabularyFinally, the transcription system with an in nitevocabulary enumerates candidate sentence in the descending order of the follow-ing evaluation ... corpora in two domains:one is a general domain in which we have a smallannotated corpus with word boundary informationand phoneme sequence for each word, and theother is a target domain in which...