word grammar approaches to linguistic analysis its explanatory power and applications

... fluorescently-labeled anti-phosphoserine and antiphosphotyrosine antibodies was developed Due to its sensitivity, high specificity and the short incubation time needed to detect phosphorylation, this ... immobilization onto avidin functionalized slides The advantage of avidin/biotin linkage over His-tag/Ni-NTA strategies for protein immobilization is highlighted by its ability to withstand a variety ... methods, and may find wider uses in high-throughput kinase screenings The site-specific immobilization of proteins is even more critical since their active site needs to be accessible to the substrates...

Ngày tải lên: 16/09/2015, 17:14

... MBP and GST, and probed with Cy3-anti-EGFP, Cy5-anti-MBP and FITC-anti-GST, respectively Three corresponding non-biotinylated proteins were also spotted onto the same slide, as controls, and ... 7.4, and spotted onto the avidin functionalized slides using an ESI SMA arrayer (Toronto, Canada) No incubation was necessary before the slide were further processed by washing with PBS and drying ... purification protocol has found wide applications in protein engineering where the expressed protein ligation (EPL) strategy is utilized to incorporate non coded amino acid sequences into a protein...

Ngày tải lên: 16/09/2015, 17:14

... attached the substrates to the microwells and incubated them with the kinase and radioactively-labeled ATP After phosphorylation had taken place, the kinases and ATP were washed away and the microwells ... range of substrates and kinase activity as well as antigen-antibody or ligand-receptor interaction and so on.13,14 However, the development of these technologies is still in its infancy since some ... Phosphorylated (p-Tyr) and non-phosphorylated tyrosine (Tyr) were arrayed onto amine-functionalized slides and probed with (a) FITC-labeled anti-phosphotyrosine and (b) and anti-phosphoserine...

Ngày tải lên: 16/09/2015, 17:14

... sacrificial layer Photoresist Photoresist coated UV light photomask Photoresist exposed Photoresist and sacrificial layer removed in mask pattern Glass etched Photoresist and sacrificial layer ... etched into the substrate in a dilute HF/NH4F bath Because the photoresist used to mask wet etches does not adhere well to glass, a sacrificial layer that adhere well to both glass and photoresist ... positive photoresist is spin-coated on top of the substrate, and the channel design is transferred to the substrate using a photomask Following exposure and development of the photoresist, the...

Ngày tải lên: 16/09/2015, 17:14

... work, and how cells form organisms The goal is not simply to provide a catalogue of all the genes and information about their function, but to understand how the components work together to direct ... acid to protein-based arrays A major challenge remains the rapid and efficient isolation of high affinity and specificity protein ligands In addition, on the contrary to DNA proteins tend to associate ... transferred to the microchip format in which case capillaries need to be derivatized to remove the electroosmotic flow to allow for efficient size based separation of nucleic acids Matrix-free DNA analysis...

Ngày tải lên: 16/09/2015, 17:14

... negative and positive-strand DWV RNA in tissues of symptomatic and TaqMan Real-Time qRT-PCR for quantification of negative and positive-strand DWV RNA in tissues of symptomatic and asymptomatic ... legs, head, thorax and abdomen were individually dissected out from symptomatic and asymptomatic bees and subjected to TaqMan real-time qRT-PCR coupled with biotinylated primer and magnetic beads ... of DWV RNA Total RNAs extracted from bees with deformed wings The negative and positive-strand cDNAs that were generated by biotinylated forward (Lane and 6) and reverse primers (Lane and 7) in...

Ngày tải lên: 20/12/2015, 08:30

... and (ii) are treated in Sections and 6, respectively Sections and are devoted to step (iii) Sections and 10 are concerned with Steps (iv) and (v) Section 11 deals with steps (vi) and (vii), and ... techniques with the modular approach to show that the only perfect powers in the Fibonacci sequence are 0, 1, and 144 and the only perfect powers in the Lucas sequence are and Introduction Wiles’ proof ... Theorems and are as follows: (i) We associate Frey curves to putative solutions of the equations Fn = y p and Ln = y p with even index n to Frey curves and apply level-lowering This, together...

Ngày tải lên: 06/03/2014, 08:21

... M・ADP・Pi, attaches to A (A), and exerts a power stroke, associated with release of Pi and ADP (from A to B) After the end of power stroke, M remains attached to A, taking its post -power stroke configuration ... analogous to those at the end of power stroke (B in Fig 14), and in response to applied ATP, they bind with ATP to form complex M・ADP・Pi, which is known to have average lifetime > 10s due to its slow ... configuration with bound Pi and ADP, and attaches to actin filament Current Basic and Pathological Approaches to 24 the Function of Muscle Cells and Tissues – From Molecules to Humans (solid line in...

Ngày tải lên: 28/06/2014, 15:20

... than a and less than b It | | does not include its end-points a and b An interval a b is the segment a b together with | a and b For a segment plus its end point a we use the notation a b, and when ... a ∞ and −∞ a, | | together with the point a, are respectively the infinite intervals a ∞ and −∞ a Unless otherwise specified the expressions segment and interval will be understood to refer to segments ... which is also left to the reader Let a and b be rational numbers not zero and let [x] be the set of all rational numbers between and a, and [y] be the set of all rationals between and b Then if a...

Ngày tải lên: 28/06/2014, 19:20

... 1990) Results and discussion 4.1 Landslides Inventory A landslide inventory was carried out to give a measure of the past instability of the area A total of 88 landslides were identified and mapped ... were carried out to determine the percentages of total sand and medium to fine sand which are prone to liquefaction under prolonged precipitation (Alexander 1993; Finlayson and Statham, 1980) ... contributed to and have previously caused landslides in the Ntchenachena area of northern Malawi Physical and anthropogenic factors contributed to the occurrence of landslides and rendered all the units...

Ngày tải lên: 29/06/2014, 03:20

... cytoplasm autoantibody (MPO-ANCA) mouse model C5aR, C5a receptor; MAPK, mitogen-activated protein kinase; PI3Kγ, phosphatidylinositol kinase-gamma; Th17, T helper 17; TLR4, Toll-like receptor ... heterogeneous in their response to inflammatory stimuli, and this is most likely due to organ- and function-specific adaptations [44] With this in mind, it will be interesting to compare the phenotype ... vitro to produce oxygen radicals [51], proinflammatory cytokines, and chemokines [52] In addition, macrophages are important cellular components of the inflammatory infiltrate in vasculitic lesions and...

Ngày tải lên: 12/08/2014, 15:22

Ngày tải lên: 09/11/2015, 08:58

... environment, hydrological and biogeochemical dynamics in complex landscapes, and land-water and land-atmosphere interactions on various scales To adequately understand and predict such phenomena ... manifest itself in terms of various patterns of land use and land cover, topography, hydrology, soils, climatic conditions, and biological factors For example, extrapolation of plot-scale data to the ... scaling approaches and several more specific upscaling and downscaling methods 17 J Wu, K.B Jones, H Li, and O.L Loucks (eds.), Scaling and Uncertainty Analysis in Ecology: Methods and Applications, ...

Ngày tải lên: 17/02/2014, 19:20

... Definition of word meal - Field of word meal in English and in Vietnamese equivalents - Cultural and linguistic analysis of the English word meal Chapter III Cultural and linguistic analysis of ... of word meal In our opinion, field of word meal is all the words relating to it mainly including eating and drinking They are parts of the word meal and resemble words in that they combine together ... together To illustrate in an easy way, we would like to list field of word meal in English and Vietnamese equivalents according to its meaning and function as follow: Field of word meal in English and...

Ngày tải lên: 15/04/2013, 15:11

... knows the words bottle and factory, and that the compound bottle factory refers to a particular kind of factory, not to a particular kind of bottle: it is the rightmost of the two word constituents ... morphology and phonology Morphology and syntax: demarcation and interaction 185 Morphology and semantics V 153 207 Morphology and Mind 229 10 Morphology and psycholinguistics 231 11 Morphology and language ... between these two sets of words The word buyer is a complex word since it can be decomposed into the constituents buy and -er The word buy, on the other hand, is a simplex word, because it cannot...

Ngày tải lên: 01/09/2013, 11:31

... SELECT FROM WHERE VIEW Keywords AS siteid, docid, word, COUNT(*) AS wordcount Documents BY siteid, docid, word; DISTINCT siteid Keywords (word = ‘IBM’ OR word = ‘Google’) AND wordcount > 5; A modern ... required us to (1) write to a custom tuple object using Hadoop’s API, (2) modify our data loader program to transform records to compressed and serialized custom tuples, and (3) refactor each benchmark ... both DBMS-X and Vertica are shown in Figure 10 as stacked bars, where the bottom segment represents the time it took to execute the UDF/parser and load the data into the table and the top segment...

Ngày tải lên: 19/02/2014, 12:20

... transliterations tend to try to represent all phonemes and often add vowels to a final consonant to form a new syllable, whereas CNA transliteration tends to be shorter and may simply leave out ... extracting and comparing terms from similar linguistic context based on CGC and WSE tools This proposal has great potential of increasing robustness of future NER work by enabling discovery of new and ... CGC was segmented and POS tagged using the Academia Sinica segmentation and tagging system (Ma and Huang, 2006) http://wordsketch.ling.sinica.edu.tw 154 ference (WSDiff) function to pick the grammatical...

Ngày tải lên: 17/03/2014, 04:20

... significant words Significant words are words that are not in some predefined stoplist of words with high overall corpus frequency2 Once significant words are marked in a text, clusters of significant words ... paragraph as important, and the other sentences as not important We included this approach merely as a simple baseline 2.2 Word- based approaches Word- based approaches to summarization are based ... significant words are formed A cluster has to start and end with a significant word, and fewer than n insignificant words must separate any two significant words (we chose n = 3, cf Luhn (1958))...

Ngày tải lên: 17/03/2014, 06:20

... equal to the ratio of within-group differences in expression to within- and between-group differences in expression It ranges from 1.0 (no discriminatory power) to 0.0 (perfect discriminatory power) ... traditional analysis of differentially expressed genes and include: IL-8, MHC class I, regulators of TNF-α (e.g TGFβ1-induced anti-apoptotic factor 1) and granulocyte/macrophage-colony-stimulating factor ... peptides to present them to CTLs Signal transducer and activator of transcription 6, interleukin-4 induced; activates expression of the interleukin-4 receptor gene in response to interleukin-4 and...

Ngày tải lên: 09/08/2014, 01:23

... Figure The GSH/GSSG ratio of the prototype and expanded models The GSH/GSSG ratio of the prototype and expanded models The prototype model (A) and the expanded model (B) Page of 11 (page number ... pentose phosphate pathway The Ru5P produced was mainly converted to fructose 6-phosphate (F6P), and this metabolite consumed ATP to make fructose 1,6-diphosphate (FDP) The FDP production led to ... prototype model The prototype model was used to simulate the effects of G6PD deficiency G6PD is a key enzyme in the pentose phosphate pathway that converts glucose 6-phosphate into gluconolactone...

Ngày tải lên: 13/08/2014, 22:22