Proteins in food processing
... of food systems, such as solids, films, emulsions, gels The establishment in the past decade of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry ... phosphorylation site; in mouse and rat -casein, Ser18 is missing) The sequence of the phosphorylation sites is also conserved in s1 - and s2 caseins In -casein, not even the chymosin cleavage site is conserved; ... identification and analysis as well as the role of yeasts in several food products including dairy products, meat, fruit, bread and beverages Details of these books and a complete list of Woodhead' s food science, ...
Ngày tải lên: 23/05/2014, 13:34
... interacts with proteins that are described as stress granule components (SGs) We identified TcDHH1, as expected, and proteins previously identified in stress granules, such as heat shock proteins and ... USA) the supernatant (SP) were resolved by SDS ⁄ PAGE and visualized by silver staining (Fig 3A) The IP and SP samples obtained with pre-immune serum were processed in the same way and are shown ... granules Our results suggest that TcDHH1containing granules contain PABPs Indeed, PABP2 seemed to colocalize with most granules containing TcDHH1 in both unstressed epimastigotes and epimastigotes subjected...
Ngày tải lên: 15/03/2014, 23:20
... electrophoresis Mass spectrometry analysis identified six phosphorylated proteins Besides the phosphoproteins which are present in both the wild-type and mutant strains two likely substrates of StkP ... EcoRI ScaI NcoI NdeI EcoRI NaeI StuI NdeI XhoI EcoRI BamHI XbaI SacII BamHI XbaI stkP expression stkP expression stkP expression stkP mutagenesis stkP expression phpP expression phpP expression ... 5.1 4.9 5.2 5.0 spr spr spr spr spr spr Cell wall biosynthesis Transcription Proteosynthesis Glycolysis Pentose metabolism Glycolysis FEBS Journal 272 (2005) 1243–1254 ª 2005 FEBS 1417 0215 0764...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo lâm nghiệp: " Growth and mineral content of young chestnut trees under controlled conditions of nutrition" ppsx
... cut away Peatlands Silva Fen- nica 29, 21-40 lyengar ER, Rcddy MP (1993) Crop response to salt stress: seawater application and prospects In: Handbook of Plant and Crop Stress (M Pcssarakli, cd), ... le développement Forestier, Paris Brethes A, Nys C (1975) Effets des résineux sur la fertilité des sols Difficulté des reeherches et premiers résultats Sci Sol 1, 3-18 Chanson B, Leban JM, Thibaut ... stem) and root samples, whereas they are higher in bark and then in leaves for Ca and inversely for Mg, Mn and K These results are in agreement with those reported in other species such as Scots...
Ngày tải lên: 08/08/2014, 18:22
Báo cáo khoa học: "gelling agents on growth, mineral composition and naphthoquinone content of in vitro explants of hybrid walnut tree (Juglans regia x Juglans nigra)" pdf
... remained constant (table III) This suggests a substitution of K by Na, probably due to a disturbance in the K selectivity mechanisms Van Steveninck (1978) showed that exogenously applied abscissic acid ... Naphthoquinones and flavonols associated with walnut rejuvenation and involved in different physiological processes Plant Physiol (suppl) 93, 147 Plantes Tempérées et Tropicales Lavoisier, Paris, 9-74 ... embryos Ann Sci For 46 suppl, 113116 PC (1983) Effects of agar brand and concentration on the tissue culture medium Physiol Plant 59, 270-276 Debergh DiCosmo F (1984) Stress and secondary metabolism...
Ngày tải lên: 08/08/2014, 23:22
Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
... analysis as described in Materials expression and protein synthesis and methods (b) Morphometric analysis of cathepsin K immunostaining (a, b) Data are expressed as median and range and are presented ... efficiencies for the test genes were the same as those for the GAPDH gene Statistical analysis Unless otherwise specified, values are expressed as the median with the range in parentheses Statistical ... expression and protein synthesis (a) mRNA levels, as determined by real-time quantitative PCR analysis as described in Materials synthesis and methods (b) Morphometric analysis of ADAMTS-4 immunostaining...
Ngày tải lên: 09/08/2014, 06:23
báo cáo khoa học: " Effect of filtration on morphine and particle content of injections prepared from slow-release oral morphine tablets" ppt
... glass microscope slide or cover, since they did not form on acid-washed glass or plastic slides However, there is a significant risk of formation of morphine crystals in the tablet extracts,, and ... Figure Particles less than μm in injection mixtures Numbers of particles (in thousands) estimated to be in the total injection volumes The unfiltered extracts were passed successively through ... Acknowledgements The authors are grateful to Inn Chua for assistance in preparing the photomicrographs, and to Tania Hunt from the Tasmanian Council on AIDS, Hepatitis and Related Diseases for assisting...
Ngày tải lên: 11/08/2014, 18:20
Báo cáo y học: " Elevated expression of both mRNA and protein levels of IL-17A in sputum of stable Cystic Fibrosis patients" potx
... controls Cytokine levels in sputum Protein measurements in sputum samples The BD™ CBA Flex Set System and buffer master kit (BD Biosciences) was used to measure protein levels of IL-17A in the sputum ... Primers and probes for IL-17A and IL-23 were previously published (IL-17A [9]; IL-23 [10]) cDNA plasmids expressing linear amounts of the target gene, were used as a standard 1 8S rRNA was used as ... the percentages on cytospins with the total cell count number counted after isolation Statistical analysis mRNA measurements in sputum samples Results RNA was isolated using the RNeasy mini kit...
Ngày tải lên: 12/08/2014, 13:22
Timing and information content of insider trades, before and after the sarbanes oxley act of 2002
... purchases (1.48%) This suggests that, after SOX, disclosures of insider purchases in financially distressed firms signal good news Overall, for purchases, the multivariate results suggest that ... dates The analysis so far ignores stock price patterns between insider transactions and their filings, especially in the pre-SOX period The next set of results addresses this issue Figure plots ... post-SOX, which suggests that the greater dispersion of post-SOX filings partly explains the less negative returns observed around Form filings of sales after SOX Finally, I investigate cross-sectional...
Ngày tải lên: 30/09/2015, 17:19
Báo cáo sinh học: "Genetic parameters for lactation traits of milking ewes: protein content and composition, fat, somatic cells and individual laboratory cheese yield" ppt
... estimates for milk composition traits in Sarda dairy sheep using a bivariate animal model, Small Ruminant Res 25 (1997) 7984 [43] SASđ Users Guide: Statistics, Version 8, SASđ Inst Inc., Cary, USA, ... Ph.D Diss., Leún University, Leún, 2000 [39] Pellegrini O., ẫtudes des relations entre les caractộristiques physico-chimiques du lait de brebis et ses aptitudes fromagốres, Ph.D Diss., Institut ... farmers may also consider including this trait when selecting service sires for their ocks, as is usually done in dairy cattle (removal of sires when their daughters are predisposed to high SCC)...
Ngày tải lên: 14/08/2014, 13:21
Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... Turkey-Kramer post test All analyses were performed using SPSS version 11.0 (SPSS Inc., USA) P
Ngày tải lên: 25/10/2012, 11:10
Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf
... and after immersion in hot water or soup for In this method, texture was assessed as a single score representing a balance of textural properties: springiness, firmness or hardness, smoothness, ... viscosity and holding strength; final viscosity (FV), highest viscosity during 50°C cooling stage; and setback (SB), difference between final viscosity and holding strength FSV Tests FSV was ... previous pass The sheet was then passed through a no 20 cutting roll to produce noodle strands with cross-sectional dimensions of 1.5 × 1.4 mm The strands were cut into 25-cm lengths, dusted with starch,...
Ngày tải lên: 16/12/2012, 15:25
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... measurements based on four time samples using the DE-81 (DEAE-cellulose) filter paper assay adopted from the deoxynucleoside kinase assay method [44] with tritium-labeled bases as substrates The standard ... alignments were performed using clustalw [39] and ESPript [40] Superpositions used in the text and figures were made using the ssm superposition algorithm in coot [33,41] The coordinates and structure ... substrate specificity of PRTFDC1 was further characterized using a radiochemical assay with tritium-labeled bases as substrates, whereas the structural basis for substrate recognition and activity was...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... and cytokine-stimulated cells, as described previously [28] The first strand of cDNA was synthesized from lg of total RNA using MMLV reverse transcriptase (Promega, Madison, WI, USA) and oligo(dT) ... blotting and measurements of luciferase activity were examined for statistical significance using Student s t test All values are means ± SD (n = 3) 7398 Acknowledgments The study was supported ... endogenous IL-1b transcript level, we used primary cultures of human fibroblasts These cells are a good model to study inflammatory processes, because, in contrast to HepG2 cells, expression of...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc
... was expressed and puried as described elsewhere [32] Protein was more than 99% pure as judged by SDS protein-denaturing gels Also, mass spectrometry analysis was performed in a MALDITOF instrument, ... the studies with this construction as its structure, as observed by NMR (J L Neira, unpublished results), is similar to that found in other members of the HPr family and the His6-tag is disordered ... structures of HPr proteins from several species have been described by NMR spectroscopy [1719 and references therein] and X-ray diffraction [20,21] Those structures show a classical open-face b-sandwich...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... L-glutamine and 100 mgÆL)1 of penicillin/streptomycin Caco-2 cells were used between passages 50 and 60 Sheep blood in Alsever s solution was purchased from TCS Biosciences Ltd., Singapore Code Name Sequence ... with a sweep width of 5000 Hz ROE cross-peak volumes were measured using ROESY spectra with 300 ms spin-lock times and NOESY cross-peak volumes for hexapeptides were measured at 200 ms mixing ... mean square deviations (rmsd) between each possible pair of structures, and was divided into several conformational families The average structure was taken from each family and chosen as starting...
Ngày tải lên: 19/02/2014, 13:20
Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... exchange factors (GEFs) and GTPase-activating proteins Mammalian Son of sevenless (Sos1) is a ubiquitous Ras GEF and activates Ras following the stimulation of receptor tyrosine kinases, e.g the ... Various GPCRs can also induce Ras activation via several classes of GEFs [2,3] Activation of phospholipase C through Gq-coupled receptors, with subsequent increased levels of inositol1,4,5-trisphosphate, ... of Ras-GRF1 [12,13] The residues Ser916 and Ser898 in the mouse and rat sequences, respectively, are homologous PKA phosphorylation sites [14] Although forskolin-induced phosphorylation of Ser916...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... (30 steps) The relaxed structure obtained was nally minimized using steepest descents and conjugate gradients algorithms This nal position for the P-pyridoxyl group was used as a starting structure ... the presence of Mg2+ Steady state and time resolved uorescence quenching Time resolved emission experiments were carried in the presence of several combinations of substrates and metal ions In ... of substrate, and s1 ẳ 6.10 ns (51%), and s2 ẳ 1.14 ns in the presence of PEP and Mn2+ Bottom: distribution of residuals using the crystalline coordinates of the free E coli PEP carboxykinase...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... condition was compared to its corresponding control a Students pooled two-sample t-test was used In all cases signicance was assumed if P < 0.05 RESULTS Formation of peptide and protein peroxides Photolysis ... N-Ac-Trp-OMe, and 130180 lM for lysozyme Data are means SD; where no error bar is visible it is obscured by the symbol Statistical analysis between conditions was by a Students pooled twosample t-test, ... Cys residues A previous study has shown that peroxides formed by O2 on peptides and proteins are not removed by catalase [25], and this has been conrmed in the present study These peroxides are...
Ngày tải lên: 21/02/2014, 03:20