with reduced enzymatic activity
Báo cáo y học: "Serum cholesterol concentration associated with aspirin esterase activity in older people: preliminary data"
Ngày tải lên :
26/10/2012, 09:39
...
population did not include subjects with malnutrition
nor there were any with nutritional modifications
during the interventional period. More studies with
larger sample sizes and longer ... height measured
in light indoor clothing without shoes. Serum total
cholesterol and plasma glucose were enzymatically
determined. Serum aspirin esterase activity was
measured kinetically by a ... serum total cholesterol concentrations are
clearly associated with those of aspirin esterase activ-
ity during a period of physical activity modification in
an older population. This finding suggests...
4
609
1
Tài liệu Báo cáo khoa học: A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity ppt
Ngày tải lên :
19/02/2014, 02:20
... displays GPX activity
FEBS Journal 274 (2007) 3846–3854 ª 2007 The Authors Journal compilation ª 2007 FEBS 3847
A novel dicyclodextrinyl diselenide compound with
glutathione peroxidase activity
Shao-Wu ... the
GPX-like activity assay. Activity was determined by the
decrease of NADPH absorption at 340 nm (e
NADPH
¼
6220 m
)1
cm
)1
). Background absorption of the noncatalytic
reaction was run without ... compounds with gluta-
thione peroxidase activity. J Am Chem Soc 111, 5936–
5939.
12 Iwaoka M & Tomoda S (1994) A model study on the
effect of an amino group on the antioxidant activity of
glutathione...
9
491
0
Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt
Ngày tải lên :
19/02/2014, 12:20
... formation is lower in the presence of the
antibody, with a decrease in the rate constant of 7% being
observed with hydroxylamine 1 andof>40%withthe
more bulky hydroxylamine 2.
The amount of ... Tresca, J.P., Ricoux, R., Pontet, M. & Engler, R. (1995) Com-
parative activity of peroxidase–antibody conjugates with periodate
and glutaraldehyde coupling according to an enzyme immuno-
assay. ... & Pratt, J.M. (1987) Hemes and
hemoproteins. 3. The reaction of microperoxidase-8 with cyanide:
comparison with aquocobalamin and hemoproteins. J. Inorg.
Biochem. 29, 77–91.
23. Dai, L. &...
7
447
0
Tài liệu Báo cáo khoa học: A selenium-containing single-chain abzyme with potent antioxidant activity docx
Ngày tải lên :
21/02/2014, 00:20
... CCO
activity. Over 60 min, CCO activity in the damage group
decreased from 0.356 ± 0.012 to 0.208 ± 0.010 U per mg
protein, i.e. by % 41.6%. Figure 3 shows that CCO
protection increased with ... liver.
Arch. Biochem. Biophys. 340, 59–63.
Ó FEBS 2003 Se-containing abzyme with potent antioxidant activity (Eur. J. Biochem. 270) 4331
with oxidation of cytochrome c in the sample cell, into
which 5 lL10m
M
K
3
Fe(CN)
6
was ... protect themselves from oxidative injury, aerobic
cells have evolved an enzymatic and nonenzymatic defense
system. The enzymatic antioxidant system is mainly
composed of glutathione peroxidase...
6
400
0
Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
Ngày tải lên :
21/02/2014, 03:20
... enzymes with high alkalinity.
Several mutant enzymes showed drastically improved
stability in solutions containing SDS (Fig. 4). ALP I
maintained 60% of enzymatic activity after 1 h, and 20%
of activity ... (3.8 · 25 cm) equili-
brated with the same buffer. The enzyme active fraction was
eluted with a 0–1.0
M
NaCl linear gradient and pooled. The
active fraction was dialysed with 10 m
M
Mes at pH 6.5
containing ... enzymes. They lost the activity within 10 min as did
wild-type ALP I.
Resistance to surfactants
Residual activities of the mutant enzymes were measured
after incubation with 0.1% SDS in 0.1
M
H
3
BO
3
/Na
2
CO
3
/
KCl...
9
489
0
Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
Ngày tải lên :
22/02/2014, 07:20
... incubated for 48 h with 5 l
M
11-deoxy-
corticosterone (DOC) including 4 nCiÆmL
)1
[
14
C]DOC. Metabolites
were analysed by TLC. Enzymatic activity is given as relative radio-
activity. White bars ... combi-
nation with [a-
35
S]dCTP followed by autoradiography with
Hyperfilm
TM
MP (Amersham).
Southern blotting
Genomic DNA was digested with the appropriate enzymes,
extracted twice with phenol/chloroform ... synthesized by CYP11B2 in comparison with
CYP11B1 (Fig. 4C). It is noteworthy, that CYP11B2
displayed a higher enzymatic activity than CYP11B1 based
on 11b-hydroxylase activity. These differences were...
9
671
0
Báo cáo khóa học: A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum docx
Ngày tải lên :
07/03/2014, 15:20
... A new UV-B absorbing mycosporine with photo protective activity
from the lichenized ascomycete
Collema cristatum
Avital Torres
1
, Malka Hochberg
4
, ... isocratic elution with 60% EtOH – 40%
MeOH, to yield 50 mg of a pale yellow powder. Purity of
the isolated compound was confirmed by HPLC analysis,
which showed a single sharp peak with a retention ... supplemented with 10% fetal bovine serum,
L
-glutamine 2 m
M
and antibiotics (100 U penicillin per
mL and 100 mg streptomycin per mL). Cells were grown to
80% confluence in 9 cm culture dishes, overlaid with...
5
450
0
Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
Ngày tải lên :
07/03/2014, 15:20
... low level
of NAAG-hydrolyzing activity, < 0.01% of the 44/750
variant, was associated with the 44/750_V5-His variant, and
no proteolytic activity could be detected with variants
N-terminally ... were
mixed with an equal volume of the denaturing SDS buffer and 10 lL
of the mixture was loaded onto a single lane. Activity levels are indi-
cated as follows: (+), measurable NAAG-hydrolyzing activity; ... catalytic activity, such as aminopeptidases
from Streptomyces griseus and Vibrio proteolyticus [13].
Additionally, the homology of human GCPII with the
transferrin receptor has been reported, with...
9
414
0
Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
Ngày tải lên :
16/03/2014, 18:20
... transfected
with the expression vectors encoding GRa and GPR30 (or
with the corresponding control vectors) together with the
reporter construct MMTV-tk-luc. MMTV-tk-luc promoter
activity was ... cells, the
effect of hydrocortisone on MMTV-luc activity w as almost
abrogated. Without the presence of hydrocortison, GPR30
had no effect on the activity, indicating that the effect is
glucocorticoid-dependent. ... treatment
in control cells (Fig. 5B). When the cells were transfected
with GPR30 and treated with dexamethasone, the luciferase
activity was almost abolished. GPR30 did not affect the
target promoter...
10
389
0
Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
Ngày tải lên :
22/03/2014, 16:21
... 150. IN enzymatic activity was measured as described previously
[58] and in Experimental procedures (t-test, P < 0.01). A–I with the different peptide as indicated in the figure, K without ... (Sigma), as described previously [79].
The enzymatic activity of horseradish peroxidase was
estimated by monitoring the product’s absorbance at
490 nm with an ELISA plate reader (Tecan Sunrise,
Ma
¨
nnedorf, ... indicates that the
observed peptide activity cannot be attributed solely to
the masking of a specific sequence or domain within
IN. In addition, the three peptide groups, with the
different kinetic profiles...
15
343
0
Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx
Ngày tải lên :
23/03/2014, 12:20
... with
13-cis-retinoids, although with low k
cat
values (Tables 2 and
3). Human ADH1 enzymes were not found to be active with
13-cis-retinoids, although a low activity had been previously
reported with 13-cis-retinal ... binding
pocket of ADH4 is the reason for the low activity observed
with this retinal isomer.
The interaction of 11-cis-retinal with ADH1B1 was also
studied and compared with that of ADH4 (Fig. 2A–D).
11-cis-retinal ... by immunohistochem-
istry, consistent with the ADH4 activity previously found in
this epithelium [25]. Thus, the presence in the RPE, the high
activity with 11-cis-retinol, and the specificity...
11
468
0
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