with reduced enzymatic activity

Ngày tải lên: 30/03/2014, 01:20

... population did not include subjects with malnutrition nor there were any with nutritional modifications during the interventional period. More studies with larger sample sizes and longer ... height measured in light indoor clothing without shoes. Serum total cholesterol and plasma glucose were enzymatically determined. Serum aspirin esterase activity was measured kinetically by a ... serum total cholesterol concentrations are clearly associated with those of aspirin esterase activ- ity during a period of physical activity modification in an older population. This finding suggests...

Ngày tải lên: 26/10/2012, 09:39

... displays GPX activity FEBS Journal 274 (2007) 3846–3854 ª 2007 The Authors Journal compilation ª 2007 FEBS 3847 A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity Shao-Wu ... the GPX-like activity assay. Activity was determined by the decrease of NADPH absorption at 340 nm (e NADPH ¼ 6220 m )1 cm )1 ). Background absorption of the noncatalytic reaction was run without ... compounds with gluta- thione peroxidase activity. J Am Chem Soc 111, 5936– 5939. 12 Iwaoka M & Tomoda S (1994) A model study on the effect of an amino group on the antioxidant activity of glutathione...

Ngày tải lên: 19/02/2014, 02:20

... formation is lower in the presence of the antibody, with a decrease in the rate constant of  7% being observed with hydroxylamine 1 andof>40%withthe more bulky hydroxylamine 2. The amount of ... Tresca, J.P., Ricoux, R., Pontet, M. & Engler, R. (1995) Com- parative activity of peroxidase–antibody conjugates with periodate and glutaraldehyde coupling according to an enzyme immuno- assay. ... & Pratt, J.M. (1987) Hemes and hemoproteins. 3. The reaction of microperoxidase-8 with cyanide: comparison with aquocobalamin and hemoproteins. J. Inorg. Biochem. 29, 77–91. 23. Dai, L. &...

Ngày tải lên: 19/02/2014, 12:20

... CCO activity. Over 60 min, CCO activity in the damage group decreased from 0.356 ± 0.012 to 0.208 ± 0.010 U per mg protein, i.e. by % 41.6%. Figure 3 shows that CCO protection increased with ... liver. Arch. Biochem. Biophys. 340, 59–63. Ó FEBS 2003 Se-containing abzyme with potent antioxidant activity (Eur. J. Biochem. 270) 4331 with oxidation of cytochrome c in the sample cell, into which 5 lL10m M K 3 Fe(CN) 6 was ... protect themselves from oxidative injury, aerobic cells have evolved an enzymatic and nonenzymatic defense system. The enzymatic antioxidant system is mainly composed of glutathione peroxidase...

Ngày tải lên: 21/02/2014, 00:20

... enzymes with high alkalinity. Several mutant enzymes showed drastically improved stability in solutions containing SDS (Fig. 4). ALP I maintained 60% of enzymatic activity after 1 h, and 20% of activity ... (3.8 · 25 cm) equili- brated with the same buffer. The enzyme active fraction was eluted with a 0–1.0 M NaCl linear gradient and pooled. The active fraction was dialysed with 10 m M Mes at pH 6.5 containing ... enzymes. They lost the activity within 10 min as did wild-type ALP I. Resistance to surfactants Residual activities of the mutant enzymes were measured after incubation with 0.1% SDS in 0.1 M H 3 BO 3 /Na 2 CO 3 / KCl...

Ngày tải lên: 21/02/2014, 03:20

... incubated for 48 h with 5 l M 11-deoxy- corticosterone (DOC) including 4 nCiÆmL )1 [ 14 C]DOC. Metabolites were analysed by TLC. Enzymatic activity is given as relative radio- activity. White bars ... combi- nation with [a- 35 S]dCTP followed by autoradiography with Hyperfilm TM MP (Amersham). Southern blotting Genomic DNA was digested with the appropriate enzymes, extracted twice with phenol/chloroform ... synthesized by CYP11B2 in comparison with CYP11B1 (Fig. 4C). It is noteworthy, that CYP11B2 displayed a higher enzymatic activity than CYP11B1 based on 11b-hydroxylase activity. These differences were...

Ngày tải lên: 22/02/2014, 07:20

... A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum Avital Torres 1 , Malka Hochberg 4 , ... isocratic elution with 60% EtOH – 40% MeOH, to yield 50 mg of a pale yellow powder. Purity of the isolated compound was confirmed by HPLC analysis, which showed a single sharp peak with a retention ... supplemented with 10% fetal bovine serum, L -glutamine 2 m M and antibiotics (100 U penicillin per mL and 100 mg streptomycin per mL). Cells were grown to 80% confluence in 9 cm culture dishes, overlaid with...

Ngày tải lên: 07/03/2014, 15:20

... low level of NAAG-hydrolyzing activity, < 0.01% of the 44/750 variant, was associated with the 44/750_V5-His variant, and no proteolytic activity could be detected with variants N-terminally ... were mixed with an equal volume of the denaturing SDS buffer and 10 lL of the mixture was loaded onto a single lane. Activity levels are indi- cated as follows: (+), measurable NAAG-hydrolyzing activity; ... catalytic activity, such as aminopeptidases from Streptomyces griseus and Vibrio proteolyticus [13]. Additionally, the homology of human GCPII with the transferrin receptor has been reported, with...

Ngày tải lên: 07/03/2014, 15:20

... transfected with the expression vectors encoding GRa and GPR30 (or with the corresponding control vectors) together with the reporter construct MMTV-tk-luc. MMTV-tk-luc promoter activity was ... cells, the effect of hydrocortisone on MMTV-luc activity w as almost abrogated. Without the presence of hydrocortison, GPR30 had no effect on the activity, indicating that the effect is glucocorticoid-dependent. ... treatment in control cells (Fig. 5B). When the cells were transfected with GPR30 and treated with dexamethasone, the luciferase activity was almost abolished. GPR30 did not affect the target promoter...

Ngày tải lên: 16/03/2014, 18:20

... 150. IN enzymatic activity was measured as described previously [58] and in Experimental procedures (t-test, P < 0.01). A–I with the different peptide as indicated in the figure, K without ... (Sigma), as described previously [79]. The enzymatic activity of horseradish peroxidase was estimated by monitoring the product’s absorbance at 490 nm with an ELISA plate reader (Tecan Sunrise, Ma ¨ nnedorf, ... indicates that the observed peptide activity cannot be attributed solely to the masking of a specific sequence or domain within IN. In addition, the three peptide groups, with the different kinetic profiles...

Ngày tải lên: 22/03/2014, 16:21

... with 13-cis-retinoids, although with low k cat values (Tables 2 and 3). Human ADH1 enzymes were not found to be active with 13-cis-retinoids, although a low activity had been previously reported with 13-cis-retinal ... binding pocket of ADH4 is the reason for the low activity observed with this retinal isomer. The interaction of 11-cis-retinal with ADH1B1 was also studied and compared with that of ADH4 (Fig. 2A–D). 11-cis-retinal ... by immunohistochem- istry, consistent with the ADH4 activity previously found in this epithelium [25]. Thus, the presence in the RPE, the high activity with 11-cis-retinol, and the specificity...

Ngày tải lên: 23/03/2014, 12:20

Ngày tải lên: 23/03/2014, 15:21

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 18/06/2014, 22:20

Ngày tải lên: 20/06/2014, 04:20

Ngày tải lên: 21/06/2014, 08:20

Ngày tải lên: 22/06/2014, 22:20