whole cell or whole animal studies for protein function

... Valarie Tan, for performing the mice embryo microinjections; Sumantra Chatterjee and Adrian Lim, for performing the zebrafish embryo microinjections; Yang Sun, for technical support for the microarrays; ... are: Whole organs, tissues or biopsies Microdissected tissue Primary cell cultures Established immortal cell lines Fluorescence Activated Cell Sorted (FACS) cells Magnetic Activated Cell Sorting ... the majority of liver tissue and hence whole liver can be used as a good proxy, the same cannot be argued for lens or beta-islet cells Lens cells and beta-islet cells form a small proportion...

Ngày tải lên: 11/09/2015, 09:59

... overlaps For instance, a book for the Toll Interleukin Receptor (TIR) domain (PhyloFacts book ID: Genome Biology 2006, 7:R83 information The 'Plant Disease Resistance Phylogenomic Explorer' forms ... evidence codes are provided for each subfamily separately as well as for the family as a whole Phylogenetic trees are constructed for each protein family, using Neighbor-Joining, Maximum Likelihood ... evidence codes for sequences in the family as a whole and for individual subfamilies their predicted domain structures using, for example, PFAM or Conserved Domain [45] analysis, or by pairwise...

Ngày tải lên: 14/08/2014, 17:22

... for function prediction np is the no of known functions of protein p; mp,β is the no of functions predicted for protein p at threshold β; and kp,β is the no of functions predicted correctly for ... of information can differ substantially in nature and representation, I propose a graph-based framework to combine them for protein function prediction Each source of information is transformed ... known antibodies for a target protein and hence cannot provide interaction information for all proteins Even with complete sequence information, sequence homology can only associate functional similarity...

Ngày tải lên: 12/09/2015, 08:18

... translation for protein engineering functional protein synthesis occurs inside liposomes, it provides a platform for simulating a complex cellular activity because the product of the system is the protein, ... a strong tool for producing proteins Production of biologically active proteins In order for the cell- free translation system to produce biologically active proteins, additional proteins such ... A system for protein evolution based on cell- free translation An initial DNA library is used as the template for cell- free translation Following genotype–phenotype (RNA protein or DNA protein) ...

Ngày tải lên: 19/02/2014, 06:20

... (‘‘GATATC’’ for EcoRV and ‘‘GGATCC’’ for BamHI) which was registered by a sudden drop in the tension This study showed that the cleavage rate for EcoRV decreases with DNA tension whereas that for BamHI ... directions Two types of force, namely gradient and scattering forces, are produced since the laser light can be both transmitted and reflected from the particle The gradient force is proportional to the ... allowing the DNA to be stretched while monitoring the force (Fig 1A) using the trap potential At low external forces (< 10 pN), the DNA extension versus force curve can be well fitted by the WLC model...

Ngày tải lên: 22/06/2014, 18:20

... TPR For the melanoma data set, we obtained FPR of 1.33% for S2 and 16.8% for S3, based on the 10 ions selected by our model For the cord blood data set, we achieved FPR of 7.23% and 8% for L ... bespoke algorithms are robust for handling noisy data and cost effective for candidate marker selection For future work, studies of biomarker validation on the identified panels will be performed ... 60:20:20 A 60% of the data is used for training the network, 20% for testing (i.e early stopping criteria based on mean squared error (MSE) for ANN) and the remaining 20% for validating the model We...

Ngày tải lên: 13/08/2014, 13:21

Ngày tải lên: 16/08/2013, 10:44

... the end of the cell width of the electrode For optimal fuel cell performance, a uniform current density generation is desirable, and this could only be achieved with a non-uniform catalyst distribution, ... permeability for the gas phase (KP ) The relative permeability accounts for the reduction in pore space available for one phase due to the existence of the second phase [7] The momentum equation for the ... ⋅ u g + εDiT T ⎥ ⎦ ( ) (13) In order to account for geometric constraints of the porous media, the diffusivities are corrected using the Bruggemann correction formula [8, 9]; eff Dij = Dij ×...

Ngày tải lên: 05/09/2013, 14:58

... the formula (2N2) The letter N represents the number of the orbit, or shell For example, the first orbit can hold no more than two electrons ؋ (1)2 or 2؋1‫2؍‬ The second orbit can hold no more ... well For this reason, the grounding prong of a plug should never be cut off or bypassed THE VOLT electromotive force volt Voltage is actually defined as electromotive force or EMF It is the force ... transmitted, stored, or used in any form or by any means graphic, electronic, or mechanical, including but not limited to photocopying, recording, scanning, digitizing, taping, Web distribution, information...

Ngày tải lên: 30/12/2013, 22:11

... below 311K (100 F) For the most severe postulated accident, the volume of water in the cavity provides a passive ultimate heat sink for or more days, permitting time for restoration of the active ... conductor that would provide a path for cooling the primary system Such an effective heat transfer mechanism may obviate the need for a sump makeup valve Therefore, a calculation was performed ... valves are required for inventory for the situation where minimum inventory makeup capability is available Main Steam Line Break A Main Steam Line Break calculation was performed to investigate...

Ngày tải lên: 16/01/2014, 22:20

... EcR-encoding vectors described below; and (d) one of the USP-encoding vectors described below After transfection for h, cells were incubated with or without agonists and ⁄ or JHIII for 24 h, cells were ... quantitative differences may prove important for in vivo functions In Manduca, the presence of a B-isoform increases transcriptional activity normally mediated by the A-isoform alone, heightening the possible ... ecdysteroid receptors from various insect species suggests a basis for identifying and screening for compounds that perturb normal receptor function [12,13,15,16] Ecdysteroid receptor-mediated transcriptional...

Ngày tải lên: 18/02/2014, 08:20

... thank S Taketani for anti-HO-1 and Y FujiiKuriyama for the HRE constructs This study was supported by Grants-in-aid for Scientific Research (B), for Scientific Research on Priority Areas, and by ... Fremont, CA, USA) Assay for HO catalytic activity YN-1, K562, HeLa and HepG2 cells (2 · 107) were incubated for 48 h under normoxia or hypoxia (1% oxygen), and harvested for the assay of HO activity, ... Western blot analysis HeLa and HepG2 cells were harvested after cultivation under normoxia (N) or hypoxia (1% oxygen) for 48 h The cell extracts were prepared for western blot analysis of HO-1 and...

Ngày tải lên: 19/02/2014, 06:20

... 1025 animal is, by definition, more than the sum of any combination of alternative approaches 1026 Therefore, for reproductive toxicity testing, animal studies will remain the last resort for 1027 ... the 7th amendment of the cosmetic directive for establishing the timetable for phasing out animal testing - Foreword AtlaAlternatives to Laboratory Animals 2005;33:1-+ 1390 1391 1392 [101] Curren ... efforts to bring together existing toxicological 773 information on reproductive toxicology in an electronic format For data that are publicly 774 available (i.e not Confidential Business Information),...

Ngày tải lên: 05/03/2014, 16:20

... were performed to investigate the effect of the phosphotyrosine product on the ability of Lck to phosphorylate tyrosine substrates The radioactivity-incorporation assay was performed for peptide ... 10)4 to 98 · 10)4 s)1 for phosphorylation of the different f ITAM tyrosines Our values are lower than the kcat value calculated from published data for the PTK c-Src for phosphorylation of enolate ... enzyme [19] It was therefore assumed that the extent of phosphorylation at 30 is directly proportional to the phosphorylation rate For each set of peptide concentrations and corresponding rate values,...

Ngày tải lên: 08/03/2014, 02:20

... "Certainly sir for how many people please" Figure 3: Structure for (1) 3.2 Splitting input into well-formed parts and ill-formed parts Item (C) splits input into well-formed parts and ill-formed parts, ... ill-formed or the translation rules are insufficient The well-formed parts can be applied patterns or they can consist of one content word The ill-formed parts, which consist of one functional word ... results for JK and KJ translations more than for JE and EJ translations 4.2 Utterance splitting into sentences In order to gain a good translation result for an utterance including more than...

Ngày tải lên: 08/03/2014, 05:21

... arises for immature animals on poor quality forage, to what extent can growth be increased by providing nutrients for the rumen and extra protein for absorption? The provision of more protein for ... availability World grain availability has been affected in the past, mostly by the demand for food for humans and feed for livestock Grain for livestock, will have to compete with increasing demand for ... some studies the escape protein supplement was found to stimulate forage intake whereas in other studies, forage intake was unchanged or reduced when protein supplements were included The studies...

Ngày tải lên: 08/03/2014, 23:20

... secondary clarification is therefore of maximum importance for overall plant performance Page | 12 New technologies or innovative treatment lines for reliable water treatment for P&P and minimization ... retentate streams for Pulp & Paper • I3.1.1.4 Assessment of technologies for the elimination of inorganic compounds for Pulp & Paper Page | New technologies or innovative treatment lines for reliable ... water quality target for water re-use and which are tailored to suit product demands and standards The work described in this report concerned the laboratory and preliminary work for the implementation...

Ngày tải lên: 09/03/2014, 00:20

... scattering; the detector voltage was set to E00 for forward scattering and 600 V for FL1, and the threshold for forward scattering was set at 52 The EGFP fluorescence signal was collected using a 530 ... sample buffer for SDS ⁄ PAGE at an A600 of 20 Fractionated cell lysates were prepared by glass bead vortex homogenization for 15 Detection system for protein protein interactions Protein extracts ... fluorescence detector (FL1, 530 ⁄ 30 nm bandpass filter) and the orange fluorescence detector (FL2, 585 ⁄ 21 nm bandpass filter) The amplifier gain was set at 1.00 for forward scattering; the detector voltage...

Ngày tải lên: 16/03/2014, 01:20

... role for QR in stabilizing transcription factors and tumour suppressors were performed with cells from a narrow range of multicellular eukaryotic organisms, i.e mammalian cells of human or murine ... proteasome antibody Therefore, these authors conclude that mammalian QR and the 20S proteasome not form a protein complex, as proposed by others [39] Unfortunately, no explanation for the copurification ... major player in cellular stress responses, including cell- cycle arrest, apoptosis, chromatin remodelling and DNA repair [53] Phosphorylation of p33ING1b at Ser126 was reported to be important for...

Ngày tải lên: 16/03/2014, 02:20

... combinations in these positions were major determinants for the susceptibility of the protein to protein N-myristoylation For postgenomic studies, reliable tools for the prediction of co- and posttranslational ... would be valuable for functional assignments of functionally unknown proteins In fact, a sophisticated program for automated prediction of protein N-myristoylation from the substrate protein sequence ... 3, 6, and For example, for the amino acid at position 6, amino acid residues Leu, Ile, and Phe are known not to be permitted for S cerevisiae NMT1p [5], but were found to be permitted for rabbit...

Ngày tải lên: 16/03/2014, 16:20