whey proteins as source of bioactive peptides against hypertension

... PhD-thesis Univ of Copenhagen, Denmark 2007 doi:10.1186/1751-0147-52-15 Cite this article as: Wierup and Häggblom: An assessment of soybeans and other vegetable proteins as source of salmonella ... serovars of salmonella simultaneously occur in different parts of the food chain, which currently is the case in many Member States of the EU [1] In contrast it is easier to perform such an assessment ... ingredients to feed mills of Company A, in comparison to the mills of Company C - E, was reflected in an increased in house contamination of salmonella of the mills of Company A, also when including...

Ngày tải lên: 12/08/2014, 18:22

... and italic hybrid proteins was much lower than that of TEM– STa hybrid proteins, and the production of TEM–V3 hybrid proteins was itself much lower than that of TEM–V3P hybrid proteins It can be ... G/C ratio Proteins 0.12 Fig Enterotoxicity of the hybrid proteins measured by suckling mouse assays The suckling mouse assay was performed as described by Giannella [31] The gut ⁄ carcass ratios ... mg (TEM260–STa) of b-lactamase per liter of culture The apparent molecular masses of the different hybrid proteins as determined by SDS ⁄ PAGE were higher ( 30 000 Da) than that of TEM-1 ( 28...

Ngày tải lên: 30/03/2014, 02:20

... development of diseases Food can prevent the development of diseases I buy food that claims to contribute to my health Everyone should consider how food influence their health I only eat food for pleasure ... Opinions about food-health link Most aware of link between food and health/disease Might guide purchase and eating behaviour Results (4) Opinions & use of label health info 'Health messages can ... (X =3.6-4.0) Belief in scientific credibility of label info might contribute more regular use Results (7) Associations agreement re statements PS associations between several statements phi=0.79...

Ngày tải lên: 08/04/2014, 18:33

... IX List of Tables XI List of Figures XII List of Schemes XIV List of Abbreviations List of Publications XX XXII I Introduction: chiral organofluorine compounds I-1.Importance of organofluorine ... conclusion, as well as preliminary results and future perspective As an implication of studies towards decarboxylation/asymmetric protonation, decarboxylative addition of fluoromalonate halfesters was ... fluorine atom at C6 of the aromatic ring This substitution was found to dramatically increase potency of the leads (2  17-fold increase in DNA gyrase-inhibitory and 100-fold increase in cellular...

Ngày tải lên: 09/09/2015, 11:25

... Isolation of plasmid DNA 72 72 2.3.4.1.1 Preparation of plasmids by the modified alkaline lysis method of Birnbolm and Doly (1979) 72 2.3.4.1.2 Preparation of plasmids by the modified boiling method of ... range of clinical outcomes 1.3.3.1 Hand, foot and mouth disease (HFMD) EV71 has been increasingly recognized as the main etiological agent of HFMD which is described as a mild childhood disease ... In the last 10 years, there was an increase of epidemics and neuropathogenicity of HFMD caused by EV71, particularly in the Asia-Pacific region Before 1997, outbreaks caused by EV71 were of smaller...

Ngày tải lên: 12/09/2015, 08:19

... University of Science and Technology, Kumasi, Ghana, in partial fulfilment of the requirements for the award of the Degree of MASTER OF PHILOSOPHY IN SOIL SCIENCE BY ILIASSO ABOUBACAR DAN KASSOUA ... portion of the solution (sample) was pipetted into a volumetric flask of 25 mL 18 followed by the addition of four (4) mL of ascorbic acid solution and mixed thoroughly The volume of the solution was ... programme It was a pleasure working with you all iii TABLE OF CONTENT PAGE DECLARATION I DEDICATION II ACKNOWLEDGMENT III TABLE OF CONTENT IV LIST OF FIGURES VIII LIST OF TABLES IX LIST OF APPENDICES...

Ngày tải lên: 03/03/2016, 11:10

... was used for esterification of whey proteins fractions 2-Esterification extent The extent of esterification of proteins was quantified by the colour reaction with hydroxylamine hydrochloride as ... activity of native and esterified whey protein The antiviral activity of native and esterified whey proteins: a-lactalbumin (ALA), b-lactoglobulin (BLG) and lactoferrin (LF) was assayed against ... effect of native and esterified whey proteins fractions against H5N1 propagated in MDCK cells at 100% (1.00 MOI) level of viral infection Native proteins have exhibited different levels of inhibitory...

Ngày tải lên: 12/08/2014, 02:20

Ngày tải lên: 04/10/2012, 10:24

Ngày tải lên: 07/03/2014, 02:20

... scaffold proteins and in other proteins of multiple binding partners (without adherence to the accepted definition of scaffolds) has been suggested and analysed [2] As a result of the rapid advance of ... definition of these proteins In this article, we focus on three classes of complex-forming proteins of related function, also including anchor and docking proteins The prototype for anchor proteins ... evidence for the extensive disorder of Caskin1 and also for the class of scaffold proteins in general Overall, the level of disorder exceeds that of the functional class so far considered to be the...

Ngày tải lên: 16/03/2014, 02:20

... specificity of two antibacterial proteins involved in insect immunity Nature 292, 246–248 Zasloff M (1987) Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of ... the influence of Mg2+ and Ca2+ on the antibacterial activities of AHH peptides As shown in Fig 2B, only Zn2+ significantly increased bacterial killing Antimicrobial activities of peptides derived ... histidine-rich peptides Fig Activities of histidine-rich peptides derived from HMWK (A) Sequence of domain of HMWK and synthetic peptides used in the study are indicated (B) Heparin-binding activity of the...

Ngày tải lên: 23/03/2014, 11:20

... sequences of nuclear and chloroplast genomes have been accumulated since the report of De Las Rivas et al [15] which summarized the composition of the extrinsic proteins in different organisms Based ... against green algal and higher plant PsbQ, the presence of PsbQ in isolated PSII of E gracilis has been confirmed recently [6] The failure of cross-reaction of the thylakoid membranes from prasinophyte ... PsbP- and PsbQ-like proteins are a kind of lipoproteins but not characterized as the extrinsic PSII proteins [12] Thus, the PsbO, PsbV and PsbU proteins are the typical extrinsic proteins in cyanobacterial...

Ngày tải lên: 23/03/2014, 15:21

... engagement of an ensemble of cell surface receptors initiates a tyrosine kinase-based signaling cascade Tyrosine phosphorylation of the Vav-GEF results in the activation of downstream Rac1 GTPase Recently, ... that astrocytes express the full complement of NADPH oxidase subunits [69] In primary astrocytes cultures, NADPH oxidase activation was induced by exposure to fAβ peptides and this response was ... respiratory chain, xanthine oxidase, microsomal enzymes, cycloxygenase and lipoxygenase In response to fAβ; however, it is believed that the primary source of ROS and the source of widespread oxidative...

Ngày tải lên: 19/06/2014, 22:20

... definitions of persistent arthritis, such as nonremission defined by DAS28 (namely presenting a DAS28-3 score of at least 2.6) Non-remission defined by DAS28-3 was present in 45% of our aggressively ... Disease duration was set as positive when present for at least months Morning stiffness was set as positive when it lasted for at least 60 The swollen joint count represents the contribution of ... months, as well as by the marked decrease in disease activity over time, as assessed by the DAS28-3, the swollen joint counts, and the M-HAQ Possibly because of this relatively intensive use of DMARDs,...

Ngày tải lên: 09/08/2014, 06:22

... morphogenetic proteins Indirect immunofluorescence (red) in two areas of inflamed bursa (BMP)-2 (green) and BMP-7 (red) in two areas of inflamed bursa (a) and (b) are from cell-rich areas; (c) and ... signs of degeneration, depending on the primary disease, and was thus unrepresentative of a normal situation Nonetheless, the presence of bioactive BMP in itself supports the hypothesis of its ... factors of the TGF-β superfamily was found in all samples (some stratification was typical), with the following order of magnitude: TGF-β (in a ratio to GAPDH of 0.3) > BMP-7 (in a ratio to GAPDH of...

Ngày tải lên: 09/08/2014, 08:22

... histopathological aspect of the mucosal epithelium of the rabbit #436 that was treated with C14 (1 μM) for 10 days (× 300) (B) Vascular congestion of the mucosal epithelium of the rabbit #435 that was treated ... lymphocytes was evaluated the sixth day of the co-culture by measurement of HIV-1 p24 antigen in supernatants using capture ELISA Cytotoxicity assay The cytotoxicity of the C14 derivative against ... presence of increasing concentrations of C14 or an unique dose of T 20 (5 μM) for hours at 37°C Cells were then washed and cultured in fresh medium for days DNA was extracted and the viral DNA was...

Ngày tải lên: 10/08/2014, 05:21

... diminution of PMN extravasation in the ear of mice The diminution of neutrophils extravasation as suggested by blood PMN counts was confirmed by the reduction of neutrophil content in ear of 62.4% ... 100 μL of water or 100 μL of water-soluble hydrocortisone (10 mg/mL) The mouse ACD was performed as described previously and ear thickness was measured every day The mice's weight was measured ... or prevention of neutrophilic diseases such as, Sweet syndrome (a neutrophilic dermatose resulting of Crohn's disease complications) as well as chronic obstructive pulmonary disease [49,50] http://www.journal-inflammation.com/content/4/1/6...

Ngày tải lên: 11/08/2014, 08:21

... indicated as Table PCR parameters were performed with minor modifications Briefly, the PCR mixtures contained μl of PCR buffers, μl of dNTP, 0.5 μl of ExTaq polymerase, μg of cDNAs and 0.5 μl of each ... side effects of CFA, group of VP1+CFA showed a higher level of mRNAs of Th2 cytokines than other groups except groups of BoIL-2/BoGM-CSF/VP1 and BoIL-2/VP1 +BoGM-CSF/VP1 Groups of BoIL-2 and ... assessed by the thickness of left footpad and right footpad ratio As shown in Figure the highest level of DTH was observed in the group of BoIL-2/BoGM-CSF/VP1, followed by groups of VP1+CFA and BoIL-2/VP1+BoGM-CSF/...

Ngày tải lên: 11/08/2014, 21:21

... of whey proteins is considered to be the reason for their allergenicity [10] Therefore, modification of whey proteins to enhance or alter their biological and functional properties may increase ... Esterification The procedure of [12] was used for esterification of whey proteins fractions using >99.5% methyl alcohol, at 4°C for 10 h as follows: Native whey proteins fractions were dispersed ... suspension of oil (butterfat), protein (cassein), sugar (lactose) and a multitude of possibly bioactive trace ingredients, including minerals, enzymes and vitamins Possible modes of action of milkbased...

Ngày tải lên: 12/08/2014, 04:21

... was associated with a higher Sharp/van der Heijde score at all time points except baseline and was also associated with a higher rate of joint destruction over a period of years [17] There was ... among the most potent predictors of the outcome of RA, as measured by the rate of radiographic joint destruction There are at least four clinically important reasons to perform and compare ACPA ... diagnosis of RA coupled with rational use of disease-modifying antirheumatic drugs has been shown to have a favorable effect on the course of the disease Early and accurate diagnosis has therefore...

Ngày tải lên: 12/08/2014, 11:22