... 65.1 (0.4) 417 (47) 33 .8 4.5(Pyr1)-ONC (M23L) 58.5 (0.2) 35 5 (29) 26.7 3. 4(Met1)-ONC (M23L) 52.9 (0.08) 34 5 (32 ) 23. 2 NDaValues in parentheses are the standard errors ofthe data.bValues ... by the disappearance ofthe (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The pH was adjusted to 2.5, 8.0 and11.5 and the samples were incubated at 37 ... hydrogen bonded to the e-amino group ofthe Lys9 side-chain, which simultaneouslyinteracts with the main phosphate ofthe substrate [32 ]. The closer positioning ofthe N-terminus to the mainprotein...
... 99.9 (99 .3) 99.9 (100) 81.7 ( 83. 7)I/(I) 9.9 (1.7) 8.0 (2.4) 3. 6 (1.9) 2.8 (2.0) 2.6 (1.9) 2.4 (2.0) 3. 7 (1.9) 3. 4 (2.0)Rsymm(I)‡ (%) 5.1 (39 .9) 6.7 (30 .8) 14.7 (38 .3) 18.2 (36 .9) 23. 1 (37 .5) ... COOH-terminalend ofthe ␣ helix, contact the phosphate groups of C 735 , C 736 , and A 737 in helix 22 near the upper three-helix junction. Residue Arg64, which is located near the other end ofthe S18 ␣ ... seen in the structure of the Tth T4 RNP (Fig. 2C) and in the structure of the 30 S subunit (1).Biochemical studies ofthe S15-rRNA inter-action indicated that the upper junction andhelix 23b can...
... DNAfragment spanning the predicted first residue ofthe prodo-main (Cys22) and the last residue ofthe mature domain(Val 339 ) of brucipain was cloned in the BamHI and KpnIsites of pQE30 for expression ... of the prodomain of cathepsin F (32 %) and that of cath-epsin W (33 %) among the mammalian CPs, albeit at asignificantly lower level than with brucipain. The PCZsimilarity to the prodomains of ... Although the prodomains of cathepsins F andW share the same degree of similarity to the prodo-main of cruzipain, the similarity ofthe mature domain of cathepsin F to that of cruzipain is higher...
... by the release of the cleaved product and stabilization ofthe carbon cationby the catalytic nucleophile. The new reducing end is thentransferred to the hydroxyl group at the3 -position of the nonreducing ... followed by the transfer ofthe new reducingend t o t he nonreducing end of an acceptor molecule,with the formation of another b(1 ,3) -glycosidic bond [3] .As the anomeric configuration ofthe linkage ... study on the effects of reducing conditions on the transport of Gas1p from the ER to the G olgi, w hich had indirectly revealed the requirement of at least one disulphide bond for the exit of Gas1p...
... revised 15 January 20 03, accepted 21 January 20 03) Eur. J. Biochem. 270, 1164–1175 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 034 74.x ResultsExpression and purification ofthe NR1 domainsWe ... kinetic properties of NR1, and as we have shown for CPR and P450 BM3( 23, 24,28) the properties ofthe flavin binding domains of NR1 are likely to mimic the redox properties ofthe domainsin full-length ... 16-fold) (29). However, the domain mixture still recon-stituted only % 2% ofthe activity ofthe full length CPR. InthecaseofNR1,thekcatfor the full length enzyme is low(1 .3 s)1) and is clearly...
... dC 39 .7).Position dC dH dPGlcN I1 92.1 5.27 )1.12 54.1 3. 61 3 74.0 3. 904 71.1 2.945 71.0 3. 476a 67 .3 3.796b 67 .3 3.852 N-H 7 .32 GlcN II1Â 100.2 4.762Â 55.0 3. 54 3 72.8 3. 754Â 69.8 3. 71 ... at 33 3 K as a finer solvent for lipid A seems agood way out ofthe preparation of complicated mixtures of deuterated solvents and no degradation occurs in theseconditions. Furthermore, at 33 3 ... lacking a C10:0 (3- OH). According to the integral of the peaks in the MALDI spectrum, the pentacyl and hexacylspecies were present approximately in the same amount. The position ofthe secondary...
... March 20 03, accepted 26 March 20 03) Eur. J. Biochem. 270, 2101–2107 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 035 93. x trypsin. It is noteworthy in this respect that the majority of the proteases ... forWINDOWS, v1 .30 .00.7 (JASCO).Fig. 3. Alignment ofthe sequences ofthe second Kunitz-modules ofthe human and fugu WFIKKN proteins (wfikkn_hu_2; wfikkn_fugu_2) with the second Kunitz-domains ofthe human ... raising the possibility that the inability of WFIKKN-KU2 to inhibitthese proteases is partly due to the lack of such a favorableinteraction ofthe PÂ2 Trp with the target enzymes. The factthat the...
... 1,219 1, 038 2 ,35 1 3, 389 Maine 1 ,35 6 144 137 281 77 58 135 Maryland 74,7 23 886 730 1,616 9,422 11,640 21,062 Massachusetts 9,064 726 7 13 1, 439 37 5 431 806 Michigan 2,5 83 106 101 207201 168 36 9 Mississippi ... 27,4 63 1 13 104 217 3, 099 3, 758 6,857 Ohio 25,279 1 ,32 1 1,210 2, 531 2 ,36 6 2,624 4,990 Pennsylvania 53, 626 3, 385 3, 114 6,499 4,115 5,229 9 ,34 4** [** was 6 ,34 4 in error.**]Rhode Island 3, 670 30 4 ... as their peculiar needs demanded. In the agricultural sections the first duty ofthe teacher ofthe blacks was to show them how to get their living from the soil. This was the final test of their...
... collectionFor the Child-OIDP interview [1,25], children were firstgiven a list of common oral problems and were askedwhether they had experienced any of them within the last 3 months. Then, the single ... Validation of a French version ofthe Child-OIDPindex. Eur J Oral Sci 2005, 1 13: 355 -36 2. 32 . Bernabe E, Sheiham A, Tsakos G: A comprehensive evaluation of the validity of Child-OIDP: further evidence ... between the Child-OIDP and proxy measures were not significant. While the low value of alpha isaffected by the nature ofthe Child-OIDP index and the fact that it contains only 8 items, the lack of...