what is required for hospital admission of a minor with a psychiatric emergency
Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
Ngày tải lên :
07/03/2014, 02:20
... for a specific IMP [2] MscL pore assembly depends on YidC Experimental procedures and 5¢-CGCGCGGGATCCTTAAGCATAATCAGGAAC ATCATAAGGATAACCACCAGGAGAGCGGTTATTC TGCTCTTTC-3¢ (reverse) The EcoRI ⁄ BamHI-digested ... QuikChange site-directed mutagenesis (Stratagene, La Jolla, CA, USA) The mutagenic primers used to construct MscL R135C were 5¢-AGCAGAATAA CTGCTCTCCTGGTG-3¢ (forward) and 5¢-CACCAGGAG AGCAGTTATTCTGCT-3¢ ... characteristics of the transmembrane pairs are also critical for the conditions of membrane insertion Analysis of the biogenesis of more and more IMPs has revealed many different requirements for targeting,...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
Ngày tải lên :
16/03/2014, 13:20
... 5¢-CGCTGCAGTAAGAGCAGTAAACCCG-3¢ 5¢-GAGAATCAGTGTCGACTTCATCTATAAAAATAATAGAAGGTTTATT-3¢ 5¢-GCCCATATTCGTCGACGCGCTAACAGGTACCAGAGGAGAAGGAGAGAGCGAAGCAAGTAG-3¢ 5¢-GGGCGGATCCTCTGCTTTTCTTTATC-3¢ 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢ ... 5¢-GCGCTAATGCAACCGTAGTCAATTGATTAACGTGCT-3¢ 5¢-AGCACGTTAATCAATTGACTACGGTTGCATTAGCGC-3¢ 5¢-TTAAAAGAACCAGATTAGTCAATTGATTAACGTGCT-3¢ 5¢-AGCACGTTAATCAATTGACTAATCTGGTTCTTTTAA-3¢ 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢ ... 5¢-AAGCAAGAACAGTTCACTGCAGCTTTTGGTCAAGCAGGTAACTAGTCAATTGAT-3¢ 5¢-ATCAATTGACTAGTTACCTGCTTGACCAAAAGCTGCAGTGAACTGTTCTTGCTT-3¢ 5¢-GCGCTAATGCAACCGATAGATGTCTCTACGGAGGAC-3¢ 5¢-GTCCTCCGTAGAGACATCTATCGGTTGCATTAGCGC-3¢ 5¢-GACGACGAAACAAGAAAAGATGGCGCCATCGAGATG-3¢...
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Báo cáo khoa học: Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system potx
Ngày tải lên :
30/03/2014, 14:20
... ng of the Ad-MLP promoter and the amounts of RNAPII holoenzyme, RNAPII, activators, and GTFs indicated at the bottom of each panel (A) Analysis of the amount of transcriptional activation by Gal4-AP2 ... (D) Analysis of the amount of transcriptional activation achieved by Gal4-VP16 with purified RNAPII and the RNAPII holoenzyme (E) Analysis of the transcriptional activation function of Gal4-CTF and ... was also able to support transcriptional activation (at least fivefold, as measured with a Phosphoimager) by the mammalian Gal4-AP2 transcriptional activator protein (lane 11), indicating that...
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Báo cáo y học: "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis" pdf
Ngày tải lên :
09/08/2014, 10:20
... Proc Natl Acad Sci USA 1998, 95:13859-13864 Fujisawa K, Asahara H, Okamoto K, Aono H, Hasunuma T, Kobata T, Iwakura Y, Yonehara S, Sumida T, Nishioka N: Therapeutic effect of the anti-Fas antibody ... Yonekura A, Matsuzaki S, Yamashita S, Iwasaki K: Localization of apoptosis and expression of apoptosis related proteins in the synovium of patients with rheumatoid arthritis Ann Rheum Dis 1996, ... rheumatoid arthritis Arthritis Rheum 2004, 50:1457-1467 53 Kawanaka N, Yamamura M, Aita T, Morita Y, Okamoto A, Kawashima M, Iwahashi M, Ueno A, Ohmoto Y, Makino H: CD14+, Page of 10 (page number...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
Ngày tải lên :
15/03/2014, 10:20
... mutagenesis using HA-RPTPa WT as the template and the following forward and reverse oligonucleotides: 5¢- ATG AAG AAG AAC CAT GTT TTA CAG ATC -3¢ and 5¢ - GAT CTG TAA AAC ATG GTT CTT CTT CAT - 3¢ ... distinctive form of Noonan syndrome Nat Genet 39, 75–79 37 Razzaque MA, Nishizawa T, Komoike Y, Yagi H, Furutani M, Amo R, Kamisago M, Momma K, Katayama H, Nakagawa M et al (2007) Germline gain -of- function ... substrate, Src, and to dephosphorylate and activate it Active RPTPa-D2 is required for Src activation Materials and methods Materials and antibodies Anti-HA-tag (12CA5), anti-Src (327) Igs and anti-RPTPa...
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Báo cáo y học: " Induction of the HIV-1 Tat co-factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs" pptx
Ngày tải lên :
13/08/2014, 09:20
... β-actin: AGAAAGGGTGTAACGCAACTAAGTC (reverse), CSF1R(forward): TTCTGCTGCTCCTGCTGGTG, CSF1R(reverse): ACCGTTGCTCCTGGCTTCAC, LOX1(forward): ACTGTGAAGGACCAGCCTGATG, LOX1(reverse): CCTAGAGTCGCAGCAGCCAG, ... Validation of the microarray data Cell cultures were infected with indicated shRNA lentiviral vectors for five days, treated with PMA for 24 hours, and total RNA was isolated Quantitative real-time ... microarray data are in general reliable Affymetrix microarray data were processed in three steps: 1) normalization and derivation of expression measures; 2) analysis of expression measures with a...
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Báo cáo y học: " A suboptimal 5'''' splice site downstream of HIV-1 splice site A1 is required for unspliced viral mRNA accumulation and efficient virus replication" pptx
Ngày tải lên :
13/08/2014, 09:21
... TTA TGT CAC3', A2 D3 sense, 5'GTC GAA TTC AGT AGA CCC TGA CCT AGC3', A2 D3 antisense, 5'TCA AAG CTT AAC ACT AGG CAA AGG TGG3' Amplification of viral DNA containing exon or was performed in × AmpliTaq ... reaction was extracted with phenol-chloroform, the RNA in the aqueous phase was precipitated with ethanol, and the RNA was fractionated on a 5% polyacrylamide gel containing 7M urea and 1/2X TBE at ... correlated with an approximately ten-fold decrease in p24 Gag accumulation in cell-free supernatants as measured by Western blot analysis of serial dilutions of viral supernatants (Fig 2B) As observed...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... TTC CAT GGC TTG ATC 3¢ 5¢ ATA CAC ACA AAC TCA CAA GG 3¢ 5¢ CAA CAT ATT TTA AAT AAA ATT GAC AAT 3¢ 5¢ ATA AAA ATG GTA TAC CTC ACT TGT*C 3¢ 5¢ TAA CAA TGA TTC TCT TTC TTT TAG 3¢ 5¢ AAT CTT TAT TTT ... TGG TAG GTA 3¢ 5¢ TGG CAC AAC ATT GTA CAT TCT TGG G 3¢ 5¢ GTA TGG AAG TGT GGA ATA GTT T 3¢ 5¢ GGA CAC CAC AGA TAT CCA ATA G 3¢ 5¢ CAT AAA ACT AAC AAT ACA TGT 3¢ 5¢ TAG CAG ATA TTG AAA AGG AG 3¢ ... TAC TAC AAC TT 3¢ 5¢ CAT GAA AAG CAT TTC AAA AA 3¢ 5¢ TAA AAA ACA GTG GTT ACC AA 3¢ 5¢ CTA AAG AGT ACA CTA TCC TCA C 3¢ 5¢ TTG CTG TGG TTG CCT ATC CTA 3¢ Sequencing of amplified genomic DNA fragments...
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Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
Ngày tải lên :
22/02/2014, 07:20
... phosphorylation during differentiation and IRES activation, we also used a plasmid encoding a variant form of eIF 2a in which Ser51 is replaced by an alanine residue (eIF 2a- S5 1A) As this variant protein ... phosphorylation of the alpha subunit of eukaryotic initiation factor J Virol 66, 1943–1950 27 Nagata, Y., Nagahisa, H., Nagasawa, T & Todokoro, K (1997) Regulation of megakaryocytopoiesis by thrombopoietin ... generated by RT-PCR using total RNA from K562 cells and the oligonucleotides 5¢-CCCCACTA GTAATTCCAGCGAGAGGCAGA-3¢ and 5¢-AATACC ATGGTCGCGGGAGGCTGCTG-3¢, and was ligated to the StuI–NcoI 7.5-kb fragment...
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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
Ngày tải lên :
16/03/2014, 11:20
... thank Ingrid Duschanek and Elli Gerick for excellent technical assistance, and Steen Malmmose for assistance with growing plants The Danish National Research Foundation, the Danish Veterinary and ... signal was passed via a preamplifier (Tektronix ADA40 0A) to an oscilloscope A total of 32 absorbance transients were collected with s interval and averaged for each decay curve The recorded absorbance ... confirmation that the aadA cassette has inserted in the psaJ gene M, marker; 1, total DNA from transgenic plant as template; 2, plasmid DNA used to transform the plants as template; 3, total DNA from...
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Báo cáo khoa học: Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation potx
Ngày tải lên :
16/03/2014, 13:20
... repeated in triplicate Northern blot analysis Plant total RNA was isolated by the method of Chomczynski & Sacchi, and 10 lg of purified total RNA per lane was loaded onto a 1% agarose denaturing ... lmolÆmin)1 (per mg of total protein) Luciferase from plasmid pLUC was included as an internal control in this assay, and statistical analysis was performed according to Breyne et al [55] All GUS experiments ... electrophoresis on a gradient gel, and western blot analysis was performed using antisera to hemagglutinin (HA) (Fig 5) Rep and RepA were easily detected from cells bombarded with p35SHA6HISRep or p35SHARepA...
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What is known about the effectiveness of economic instruments to reduce consumption of foods high in saturated fats and other energy-dense foods for preventing and treating obesity? docx
Ngày tải lên :
17/03/2014, 08:20
... in this review This synthesis is based on evidence from the main databases of biomedical and health economic literature through May 2006 as well as a small number of unpublished monographs of direct ... products are distinguished by initial capital letters The World Health Organization does not warrant that the information contained in this publication is complete and correct and shall not be liable ... are associated with many severe comorbidities (related illnesses), including cardiovascular disease, diabetes, gallbladder disease and cancers (including colon, endometrial, gallbladder, breast,...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
Ngày tải lên :
17/03/2014, 23:20
... analysis PCR products (base pairs) Target gene Forward primer Reverse primer Wilson gene 5¢-TGTTAAGTTTGACCCGGAAATTATC 5¢-CCGGTCAGCCAGCTGCTG 5¢-TGATGGTGGGCATGGGTCAG 5¢-TACATGGTGGTGCCGCCAGA a- actin ... volume of 50 lL of reaction mixture using the RT-PCR kit (Stratagene) PCR amplification was performed using LA Taq (Takara, Japan) in a total volume of 50 lL containing 55 pg of competitor At this ... (1993) Isolation and characterization of a human liver cDNA as a candidate gene for Wilson disease Biochem Biophys Res Commun 197, 271–277 Lutsenko, S & Kaplan, J.H (1995) Organization of P-type ATPases:...
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Báo cáo khoa học: Hydrogen bond residue positioning in the 599–611 loop of thimet oligopeptidase is required for substrate selection pdf
Ngày tải lên :
23/03/2014, 06:20
... CGCTCAGTACTATGCTTACTTGTGGAGTGAGGTG); RvRepG61 1A (CACCTCACTCCACAAGTAAGCATAGT ACTGAGCGTCGTA); FwRepG60 3A (CTTTTGGCCA CCTCGCTGCTGGCTACGACGCTCAGTAC); RvRepG60 3A (GTACTGAGCGTCGTAGCCAGCAGCGAGGTG 5614 GCCAAAAG); ... FwRepG60 4A (GGCCACCTCGCTGGTG CCTACGACGCTCAGTAC); RvRepG60 4A (GTACTGA GCGTCGTAGGCACCAGCGAGGTGGCC); FwRepG59 9A (CAACATGCCAGCCACTTTTGCCCACCTCGCT GGTGGCTACG); RvRepG59 9A (CGTAGCCACCAGC GAGGTGGGCAAAAGTGGCTGGCATGTTG); ... GAGGTGGGCAAAAGTGGCTGGCATGTTG); FwRepY605F (CCACCTCGCTGGTGGC TTCGACGCTCAG TACTATG); RvRepY605F (CATAGTACTGAGCGTCG AAGCCACCAGCGAGGTGG); FwRepY609F (GGCTA CGACGCTCAGTTCTATGGCTACTTGTGG); RvRepY609F (CCACAAGTAGCCATAGAACTGAGCGTCGT...
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Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot
Ngày tải lên :
23/03/2014, 07:20
... were as follows: cycD-F, 5¢-GGGATCCCA CATTGTATTCG-3¢; cycD-R, 5¢-ACGGAGCTTTGAAG CCAGTA-3¢; cycE-F, 5¢-AAGGTGCAGAAGACGCA CTT-3¢; cycE-R, 5¢-AATCACCTGCCAATCCAGAC-3¢; cdk4-F, 5¢-TACAACAGCACCGTGGACAT-3¢; ... Oxford Supplementary material The following supplementary material is available online: Characterization of Drosophila jumonji Fig S1 dJmj is not required for global modification of histone tails ... Df(3L)AC1 animals also exhibited larval and pupal lethality and displayed similar phenotypes as homozygous djmje03131 mutants (Fig 5A and data not shown), confirming that djmje03131 is a loss of...
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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
Ngày tải lên :
23/03/2014, 10:21
... (a) full-length SEP53, forward 5¢)3¢ CAGTC AAGCTTATGCCTCAGTTACTGCAAAAC and reverse 5¢)3¢ CATAGCTCGAGTCATGGCTTGGTGCTTCTC; (b) DCa–SEP53, forward 5¢)3¢ TGCTAGAATTCAGATC TATGAGCGAGAGTGCTGAGGGA and ... PCR amplification of this SEP53 fragment to introduce attB sequences for cloning into the Gateway cloning system (Invitrogen) were: forward 5¢-GGGGACAAGTTTGTACAAAAAAGCAG GCTCCATGCCTCAGTTACTGCAAAACATTAATGGG ... GCTCCATGCCTCAGTTACTGCAAAACATTAATGGG ATCATCGAGGCC-3¢; reverse 5¢-GGGGACCACTTTGT ACAAGAAAGCTGGGTCGGCCAGCGGCTTAAGGTT TTATTGATGCATTAGGGTAGATGGGGC-3¢ Human SEP53 gene was subcloned into the Gateway entry vector...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
Ngày tải lên :
30/03/2014, 13:20
... immunoprecipitation with an antibody raised against PAK1 Activation of PAK1 by LPA was maximal at incubation with 7.8-fold increase, and returned to basal level at 25 (Fig 1) The data indicated that LPA ... EAGLESIGHT software v3.2 (Stratagene) for data analysis, as described previously [25] Statistical analysis Results are expressed as means ± SE and an analysis was carried out by one-way Student’s ... clearly demonstrated that PAK1 activation was required for FAK phosphorylation to increase cell motility by LPA in A2 058 human melanoma cells Activation of PAK is regulated intracellularly by various...
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Báo cáo sinh học: " A conditional-lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis" pptx
Ngày tải lên :
18/06/2014, 22:20
... cells At an MOI of 0.1 or 0.5 there was an average reduction of 99.1% of infectious virus particles (Fig 2) At an MOI of there was an average reduction of 95.7%, and at an MOI of 10 there was an average ... There appeared to be an accumulation of immature viral particles, some with nucleoids, as well as the appearance of crescent shaped particles (Fig 4, panels D-F), similar to those observed by AnsarahSobrinho ... treated with DTT and NP40 to separate the envelope fraction from the core fraction and protein from each sample was separated by SDSPAGE and detected by Western blot with anti-I7L antisera As...
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Báo cáo hóa học: " The YPLGVG sequence of the Nipah virus matrix protein is required for budding" pdf
Ngày tải lên :
20/06/2014, 01:20
... release Proc Natl Acad Sci USA 1991, 88:3195-3199 Neumann G, Ebihara H, Takada A, Noda T, Kobasa D, Jasenosky LD, Watanabe S, Kim JH, Feldmann H, Kawaoka Y: Ebola virus VP40 late domains are ... GE Healthcare, Piscataway, NJ) Proteins were analyzed by SDS-PAGE and autoradiography Ebola VP40 budding assay The expression plasmids for Ebola VP40 and glycoprotein GP have been generated as ... sera from a rabbit immunized with gamma-irradiated NiV and a MAb directed against VP40 were also used Rabbit polyclonal antisera against the green fluorescent protein (GFP) was purchased (Invitrogen,...
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báo cáo hóa học:" A conditional-lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis" potx
Ngày tải lên :
20/06/2014, 04:20
... cells At an MOI of 0.1 or 0.5 there was an average reduction of 99.1% of infectious virus particles (Fig 2) At an MOI of there was an average reduction of 95.7%, and at an MOI of 10 there was an average ... There appeared to be an accumulation of immature viral particles, some with nucleoids, as well as the appearance of crescent shaped particles (Fig 4, panels D-F), similar to those observed by AnsarahSobrinho ... treated with DTT and NP40 to separate the envelope fraction from the core fraction and protein from each sample was separated by SDSPAGE and detected by Western blot with anti-I7L antisera As...
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