what animals live in the under canopy layer of the rainforest
Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx
... ‘assembly’domain and a proline-arginine rich domain at the extreme carboxy-terminal end. The GTPase domain is the most highly conserved domain within the members of the dynamin family. The functional ... characterization of the intrinsic structural anddynamic characteristics of the individual domains has the potential to throw valuable light on the interac-tions of the individual domains, and the mechanismand ... oligomerization [5]. In addition, the GED has also been shown to bind the middle domain of dynamin [7]. The above possibility of functional dissection of the dynamin protein into specific domains suggests...
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Báo cáo khoa học: Revisiting the 13C-label distribution of the non-oxidative branch of the pentose phosphate pathway based upon kinetic and genetic evidence doc
... rerouting of metabolicfluxes was observed. The incorporation of geneticknowledge into the metabolic network model for the nonoxidative branch of the PPP introduced the possi-bility of modeling the ... residue of the active site of the enzyme, leading to the formation of either g3p or e4p while leaving behind the bound di-hydroxyacetone (C3). The nitrogen atom of the Schiffbase (similar to the ... segregated pools, the 13C labeling of the C2andC3fragments (and, consequently, the labeling of the metabolites formed from these) can differ from the 13Clabeling of the single C2and C3fragment...
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A Compilation of the Messages and Papers of the Presidents Section 3 (of 3) of Volume 8: Grover Cleveland, First Term. pptx
... on the subject. That the exclusion of ChineseA Compilation of the Messages and Papers of the Presidents 68 Thursday, the 3d of September, being the anniversary of the signing of the treaty of ... in the courts orotherwise their right to the privilege of free ingress and egress which it was the purpose of the treaty to secure. In the language of one of the judicial determinations of the ... 1885, in relation to the management and conduct of the office of district attorney of the United States for the southern district of Alabama." The incumbent of this office on the 1st day of...
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Báo cáo khoa họcRe-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme docx
... than the true value, underlining the impact of quite asmall level of contamination on these results.Reassessment of the effects of the mutations oncoenzyme specificity In view of the dramatically ... HPLC, finding no contamination of NADP+byNAD+within the detection limits of HPLC. The authors, however, point out the presence of 2%NADP+ in the NAD+: this was claimed not to intro-duce ... thatapparent rates of NADP+utilization mainly reflected the reduction of con-taminating NAD+, creating an entirely false view of the initial coenzymespecificity and also of the effects of mutagenesis....
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Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
... electrophoresis.Another striking difference of the MALDI-TOF spec-tra from the modified Fe-protein in comparison to the unmodified Fe-protein is the decreased intensity of peak1625.4 m ⁄ z and the absence of ... Fe-proteins or other proteins.Results and DiscussionSite-directed mutagenesis of the target arginine of dinitrogenase reductaseAn indication for the covalent modification of onesubunit of dinitrogenase ... can beexplained again by the inability of trypsin to cleaveC-terminal to R102 due to the modification at this resi-due. However, the presence of peak 1625.5 m ⁄ z in the spectrum of the modified...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... Thus, we wereinterested in isolating the cDNA of PO-CHH andidentifying the native CHH neuropeptide in the ES,after the recent report of CHH cDNA from the ES[28]. Also, we examined the physiological ... decrease in CHH expres-sion in the ES is in proportion to that of CCAPexpression in the same tissue, as described in Chunget al. [47], but there is no change in CCAP of TG,indicating the tissue-specific ... targeted into the specific sites in which the nerve fibers of the PO innervate the anteriorramifications. On the other hand, as a neurohormone,PO-CHH may be released from the surface of the POand into...
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Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx
... DMT1 since the discovery of this gene, there has so far been no structuralcharacterization of either this integral protein or a segment of it. Analysis of the structure of membrane proteins eitherby ... of Gd3+at pH 4.0, but the degree to which the intensities recovered was lower in the presence of Gd3+,particularly for the C-terminal residues, than in the presence of the same amounts of ... with the anionic head group of SDS molecules.Whether the C-terminus has a regulative role in metaltransport remains to be investigated further.It is of interest to investigate whether the peptide...
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Báo cáo Y học: The role of the second binding loop of the cysteine protease inhibitor, cystatin A (stefin A), in stabilizing complexes with target proteases is exerted predominantly by Leu73 pdf
... Search FixingÕ of the side chains within the Leu73–Asn77 segment in the second binding loop of cystatin A. After each of these steps, the conformation of the second binding loop in the complex ... cystatin A in green and papain in blue.Residues in the second binding loop of cystatin A mutated in this workareinred.PapainresiduesinvolvedininteractionswiththecystatinAsecond-binding-loop ... the inhibition of papainand most likely also of cathepsin L. The side chains of Leu73 and Pro74 jointly contribute 45% of the totalunitary free energy of binding of cystatin A to cathepsin...
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Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
... confirmed the results obtained in the pull-down assays, showing that there is a physicalinteraction between the N- and C-terminal domains of SSIII in vitro.Mapping of the CD-binding region in the N-terminal ... important role in the promotion of starchbinding to CD, subsequently increasing its concentra-tion in the active site, and thus determining the cata-lytic efficiency of the protein for the polysaccharide.Although ... D3 domains,respectively, but not the individual starch-binding domains, are involved in the interaction with the catalytic domain. We also determined that the resi-dues W366 and Y394 in the D2...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... modulation of multiple genesinvolved in lipid metabolism [69]. The location of the core+1 ORF within the viralgenome, the findings that the core+1 ORF can beexpressed independently of the polyprotein ... pro-tein containing the first 8–11 amino acids of corefused to amino acids encoded by the core+1 ORF[15–17]. By contrast, internal translation initiation of core+1 can occur at the internal ... within the indicated timesat concentrations of 5 and 25 lm, respectively. The proteaseinhibitor cocktail for mammalian extracts (containingAEBSF, aprotinin, leupeptin, bestatin, pepstatin a...
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báo cáo hóa học: " Initial validation of the Argentinean Spanish version of the PedsQL™ 4.0 Generic Core Scales in children and adolescents with chronic diseases: acceptability and comprehensibility in low-income settings" pdf
... complete the instrument. Stepsto expand the use of the Argentinean Spanish PedsQL™ 4.0 include an alternative approach toscoring for the 2–4 year-olds, further understanding of how to increase ... illiterates. The mainimplications of these findings are that in order to use Ped-sQL™ in our public health setting, availability of trainedinterviewers during questionnaire administration needsto ... should include an alternative approach to scor-ing for the 2–4 year-olds, further understanding of how toincrease reliability for the 5–7 year-old self-report, andconfirmation of other aspects of...
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Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25