what animals live in the under canopy layer of the rainforest

... ‘assembly’ domain and a proline-arginine rich domain at the extreme carboxy-terminal end. The GTPase domain is the most highly conserved domain within the members of the dynamin family. The functional ... characterization of the intrinsic structural and dynamic characteristics of the individual domains has the potential to throw valuable light on the interac- tions of the individual domains, and the mechanism and ... oligomerization [5]. In addition, the GED has also been shown to bind the middle domain of dynamin [7]. The above possibility of functional dissection of the dynamin protein into specific domains suggests...

Ngày tải lên: 23/03/2014, 11:20

... rerouting of metabolic fluxes was observed. The incorporation of genetic knowledge into the metabolic network model for the nonoxidative branch of the PPP introduced the possi- bility of modeling the ... residue of the active site of the enzyme, leading to the formation of either g3p or e4p while leaving behind the bound di- hydroxyacetone (C 3 ). The nitrogen atom of the Schiff base (similar to the ... segregated pools, the 13 C labeling of the C 2 and C 3 fragments (and, consequently, the labeling of the metabolites formed from these) can differ from the 13 C labeling of the single C 2 and C 3 fragment...

Ngày tải lên: 23/03/2014, 15:21

... on the subject. That the exclusion of Chinese A Compilation of the Messages and Papers of the Presidents 68 Thursday, the 3d of September, being the anniversary of the signing of the treaty of ... in the courts or otherwise their right to the privilege of free ingress and egress which it was the purpose of the treaty to secure. In the language of one of the judicial determinations of the ... 1885, in relation to the management and conduct of the office of district attorney of the United States for the southern district of Alabama." The incumbent of this office on the 1st day of...

Ngày tải lên: 31/03/2014, 11:20

Ngày tải lên: 07/08/2014, 03:22

Ngày tải lên: 09/08/2014, 22:22

... than the true value, underlining the impact of quite a small level of contamination on these results. Reassessment of the effects of the mutations on coenzyme specificity In view of the dramatically ... HPLC, finding no contamination of NADP + by NAD + within the detection limits of HPLC. The authors, however, point out the presence of  2% NADP + in the NAD + : this was claimed not to intro- duce ... that apparent rates of NADP + utilization mainly reflected the reduction of con- taminating NAD + , creating an entirely false view of the initial coenzyme specificity and also of the effects of mutagenesis....

Ngày tải lên: 06/03/2014, 00:20

... electrophoresis. Another striking difference of the MALDI-TOF spec- tra from the modified Fe-protein in comparison to the unmodified Fe-protein is the decreased intensity of peak 1625.4 m ⁄ z and the absence of ... Fe-proteins or other proteins. Results and Discussion Site-directed mutagenesis of the target arginine of dinitrogenase reductase An indication for the covalent modification of one subunit of dinitrogenase ... can be explained again by the inability of trypsin to cleave C-terminal to R102 due to the modification at this resi- due. However, the presence of peak 1625.5 m ⁄ z in the spectrum of the modified...

Ngày tải lên: 07/03/2014, 02:20

... Thus, we were interested in isolating the cDNA of PO-CHH and identifying the native CHH neuropeptide in the ES, after the recent report of CHH cDNA from the ES [28]. Also, we examined the physiological ... decrease in CHH expres- sion in the ES is in proportion to that of CCAP expression in the same tissue, as described in Chung et al. [47], but there is no change in CCAP of TG, indicating the tissue-specific ... targeted into the specific sites in which the nerve fibers of the PO innervate the anterior ramifications. On the other hand, as a neurohormone, PO-CHH may be released from the surface of the PO and into...

Ngày tải lên: 16/03/2014, 06:20

... DMT1 since the discovery of this gene, there has so far been no structural characterization of either this integral protein or a segment of it. Analysis of the structure of membrane proteins either by ... of Gd 3+ at pH 4.0, but the degree to which the intensities recovered was lower in the presence of Gd 3+ , particularly for the C-terminal residues, than in the presence of the same amounts of ... with the anionic head group of SDS molecules. Whether the C-terminus has a regulative role in metal transport remains to be investigated further. It is of interest to investigate whether the peptide...

Ngày tải lên: 16/03/2014, 16:20

... Search FixingÕ of the side chains within the Leu73–Asn77 segment in the second binding loop of cystatin A. After each of these steps, the conformation of the second binding loop in the complex ... cystatin A in green and papain in blue. Residues in the second binding loop of cystatin A mutated in this work areinred.PapainresiduesinvolvedininteractionswiththecystatinA second-binding-loop ... the inhibition of papain and most likely also of cathepsin L. The side chains of Leu73 and Pro74 jointly contribute  45% of the total unitary free energy of binding of cystatin A to cathepsin...

Ngày tải lên: 17/03/2014, 10:20

... confirmed the results obtained in the pull-down assays, showing that there is a physical interaction between the N- and C-terminal domains of SSIII in vitro. Mapping of the CD-binding region in the N-terminal ... important role in the promotion of starch binding to CD, subsequently increasing its concentra- tion in the active site, and thus determining the cata- lytic efficiency of the protein for the polysaccharide. Although ... D3 domains, respectively, but not the individual starch-binding domains, are involved in the interaction with the catalytic domain. We also determined that the resi- dues W366 and Y394 in the D2...

Ngày tải lên: 22/03/2014, 21:20

... modulation of multiple genes involved in lipid metabolism [69]. The location of the core+1 ORF within the viral genome, the findings that the core+1 ORF can be expressed independently of the polyprotein ... pro- tein containing the first 8–11 amino acids of core fused to amino acids encoded by the core+1 ORF [15–17]. By contrast, internal translation initiation of core+1 can occur at the internal ... within the indicated times at concentrations of 5 and 25 lm, respectively. The protease inhibitor cocktail for mammalian extracts (containing AEBSF, aprotinin, leupeptin, bestatin, pepstatin a...

Ngày tải lên: 30/03/2014, 03:20

... complete the instrument. Steps to expand the use of the Argentinean Spanish PedsQL™ 4.0 include an alternative approach to scoring for the 2–4 year-olds, further understanding of how to increase ... illiterates. The main implications of these findings are that in order to use Ped- sQL™ in our public health setting, availability of trained interviewers during questionnaire administration needs to ... should include an alternative approach to scor- ing for the 2–4 year-olds, further understanding of how to increase reliability for the 5–7 year-old self-report, and confirmation of other aspects of...

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 20/06/2014, 15:20

Ngày tải lên: 09/07/2014, 08:17

Ngày tải lên: 09/08/2014, 10:20

Ngày tải lên: 12/08/2014, 01:22

Ngày tải lên: 12/08/2014, 02:20