wharton s jelly mesenchymal stem cells

... Int J Med Sci 2011, ligands expressed in injured tissues play important roles (2) Stem cells circulate among tissues, and stem cells migrate to the injured tissues once injury occurs Stem cell ... VonKossa staining Distribution of MSCs in vivo Gross presentations of femoral head Kodak DXS small animal imaging system is a real-time imaging system which can be used to observe the distribution ... infected MSCs was similar to that of normal MSCs Figure Induction of osteogenesis and adipogenesis of MSCs (×100) A: One week after osteogenesis induction, alkaline phosphatase staining showed positive...

Ngày tải lên: 25/10/2012, 11:18

... immunosorbent assay (ELISA) analysis To determine the reason for this lack of IL-1b and TNF-a secretion by MSCs, changes in these factors’ translation in hypoxia ⁄ SD-stimulated MSCs were investigated ... results of ELISA analysis of supernatants from MSCs after hypoxia ⁄ SD stimulation for 12 h in the presence and absence of ATP and LPS (B) Representative western blots for TNF-a expression in MSCs ... and TNFa transcriptional upregulation However, these two factors are not secreted from hypoxia ⁄ SD-stimulated MSCs unless a second signalling stimulus is present This finding suggests that the...

Ngày tải lên: 18/02/2014, 04:20

... test systems Although primary human hepatocytes are the best source of cells for such systems, their use for this purpose is limited Transcriptome in hepatic induction of AT-MSCs by donor shortage ... determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a large number of AT-MSCs ... neurogenic mesenchymal stem cells in human scalp tissue Stem Cells 23, 1012–1020 In’t Anker PS, Scherjon SA, Kleijburg-van der Keur C, de Groot-Swings GM, Claas FH, Fibbe WE & Kanhai HH (2004) Isolation...

Ngày tải lên: 30/03/2014, 04:20

... analysis of freshly digested and not cultured hFT, where we used mesenchymal stem cells markers (CD13, CD29, CD44, CD73, CD90, Stro-1, SH2, SH3 and SH4), as well as tissue specific markers (CD14, ... fragments that are usually discarded in surgical procedures may represent important sources of stem cells and their use does not pose ethical problems This is the first study to demonstrate the isolation, ... fallopian tube, and uterosacral ligaments as controls, they could not demonstrate chondrogenesis This suggests that there may be less progenitor stem cells in these tissues due to their lower burden...

Ngày tải lên: 18/06/2014, 15:20

... of stroke has seldom been discussed [19] Compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, ADMSCs have the distinct advantages of being abundant, easy to obtain ... excised Then 200-300 μL of sterile saline was added to every 0.5 g of tissue to prevent dehydration The tissue was cut into < mm3 size pieces using a sharp, sterile surgical scissors Sterile saline ... multiple-comparison post hoc test Statistical analyses were performed using SAS statistical software for Windows version 8.2 (SAS institute, Cary, Page of 16 NC) A probability value < 0.05 was considered statistically...

Ngày tải lên: 18/06/2014, 16:20

... embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy to obtain with minimal invasiveness, ... cut into < mm3 size pieces using a pair of sharp, sterile surgical scissors Sterile saline (37°C) was added to the homogenized adipose tissue in a ratio of 3:1 (saline: adipose tissue), followed ... multiple comparison procedure SAS statistical software for Windows version 8.2 was utilized (SAS institute, Cary, NC) A probability value

Ngày tải lên: 18/06/2014, 16:20

... transduction was analyzed by FACS: (E) Control MSCs, (F) GFP-MSCs, (G) SVV/GFP-MSCs (C-D): SVV expression in gene modified MSCs, (C): no SVV expression in GFP-MSCs, (D): stronger SVV expression ... passes, and then used for transplantation The efficiency of gene transduction was assessed with FACS SVV expression in modified MSCs The survivin expression was detected by immunofluorescence staining ... bottle was covered with long-spindle cells Passaged cells (mostly spindle cells) were uniformly distributed, and covered the bottom every ~ days The 3rd Passage MSCs highly expressed the surface...

Ngày tải lên: 18/06/2014, 19:20

... expressed as means ± SD Statistical analysis was adequately performed by ANOVA followed by Bonferroni multiple-comparison post hoc test Statistical analysis was performed using SAS statistical software ... marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated ... inflammatory response and oxidative stress as well as enhancement of angiogenesis Study Limitations This study has limitations First, since the current study was only designed to investigate the...

Ngày tải lên: 18/06/2014, 22:20

... MannWhitney test using SPSS software 13.0 (SPSS Inc., Chicago, IL, USA) The results are expressed as the mean value ± standard of deviation Statistical significance was set at P < 0.05 Tissue preparation ... close association between the adipogenesis of MSCs and steroid-associated ON development during early stage A close association between elevated adipogenesis of MSCs and steroid-associated osteonecrosis ... adipogenesis of MSCs was associated with steroid-associated ON extension 10 11 12 There were few published works exploring the relationship between adipogenesis of MSCs and steroid-associated ON Lee studied...

Ngày tải lên: 20/06/2014, 00:20

... transduction was analyzed by FACS: (E) Control MSCs, (F) GFP-MSCs, (G) SVV/GFP-MSCs (C-D): SVV expression in gene modified MSCs, (C): no SVV expression in GFP-MSCs, (D): stronger SVV expression ... passes, and then used for transplantation The efficiency of gene transduction was assessed with FACS SVV expression in modified MSCs The survivin expression was detected by immunofluorescence staining ... bottle was covered with long-spindle cells Passaged cells (mostly spindle cells) were uniformly distributed, and covered the bottom every ~ days The 3rd Passage MSCs highly expressed the surface...

Ngày tải lên: 20/06/2014, 04:20

... of cells present on tumor surfaces Luciferase expression in tissue lysates Tumors and selected organs (liver, spleen, intestine, kidney) after imaging were used to prepare tissue lysates Organs ... MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells ... cells (MSC-AR) We have shown that these AR-expressing MSCs (MSC-AR) have enhanced binding to erbB2-expressing cells in vitro Furthermore, we tested erbB-2 targeting of MSC-AR in model systems...

Ngày tải lên: 20/06/2014, 07:20

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Ngày tải lên: 07/08/2014, 18:21

... expressed as the mean ± SD Statistical analysis was performed using one-way ANOVA analysis followed by a posthoc LSD test The acceptance level for statistical significance was p＜0.05 Results Establishment ... manufacturer Statistical analysis Experiments were repeated three times The band from the western blot was scanned and analyzed by densitometry using the Sigma Gel System (SPSS, USA) Density values are ... expression in PC12 cells There are several possibilities for what mediates this Discussion 380 Guang-Zhen Jin et al induction First, PC12 cells can synthesize and release dopamine [8], and MSCs...

Ngày tải lên: 07/08/2014, 20:23

... tnacifingis deredisnoc saw 50.0 < p A AVONA yaw -eno a gnisu dessessa saw snaem puorg gnoma secnereffid eht fo ecnacifingis lacitsitats ehT )ASU ,SSPS( erawtfos 0.21 SSPS gnisu tuo deirrac saw sisylana ... eht retfa skeew ta dezinahtue erew sgod lla ,segnahc lacigolohtapotsih eht ssessa oT :tnemssessa lacigolohtapotsiH yrujni eht retfa skeew dna ,0 ta senalp lattigas W2T ni desserpxe snoisel yrujni ... yrosnesotamos ehT slaitnetop dekove yrosnesotamoS ± naem eht sa desserpxe saw ataD :sisylana lacitsitatS droc lanips degamad eht fo retnecipe eht ta demrofrep erew sesylana owt esehT erawtfos emas eht...

Ngày tải lên: 07/08/2014, 23:22

... mixture and invasion of fibrous connective tissues into the implant instead of vascular and host bone ingrowth In assessing the present observations, it was suggested that vascular or bone ingrowth ... canine segmental bone defects J Bone Joint Surg Am 1998, 80, 985-996 Chang YJ, Shih DT, Tseng CP, Hsieh TB, Lee DC, Hwang SM Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from ... defect Tissue Eng 1997, 3, 173-185 13 Kadiyala S, Young RG, Thiede MA, Bruder SP Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro Cell Transplant...

Ngày tải lên: 07/08/2014, 23:22

... explains the possibility of stem cells possessing a neural progenitor s characteristics A similarity between various tissues has been observed in previous studies [20,21] We also found the undifferentiated ... CD44, CD105 Also the cUCB-MSCs strongly expressed the embryo stem cells associated surface marker Table Fluorescence-activated cell sorter analysis of canine UCB -mesenchymal stem cells Marker CD4 ... Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one very critical issue for stem cell therapy [12,28,33] The purpose of this study was...

Ngày tải lên: 07/08/2014, 23:22

... regeneration [134] Mesenchymal stem cells versus embryonic stem cells Embryonic stem (ES) cells are derived from the inner cell mass of the embryonic blastocyst These cells can be maintained ... These questions apply not only to MSCs, but also to the larger field of stem- cell research, since there is no current consensus as to whether all these pools of stem cells are separate entities ... Future of mesenchymal stem cells To seriously consider the applications of MSCs for regeneration and tissue engineering, two key fundamental questions regarding these cells must be addressed: what...

Ngày tải lên: 09/08/2014, 01:21

... GFP-positive cells and immunopositivity for matrix proteins in the same cells Results Identification of injected mesenchymal stem cells No GFP-positive cells were observed within control tissue ... those injected into NP tissue explants Monolayer MSCs displayed no Sox-9 immunopositivity, but upon injection into NP tissue explants MSCs were immunopositive (as assessed by immunofluorescence ... representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue explants at 48 hours, week, weeks and weeks post injection Scale bar = 570 μm...

Ngày tải lên: 09/08/2014, 01:22

... different samples of cells from each tissue ± SD by FACS analysis, suggesting that this marker was not specific for synoviocytes Expression of MHC class I molecules on BM-MSC and synovial fibroblasts ... whereas the median expression across all samples is black The software groups genes by similarities in their pattern of expression, and groups samples by similarities in their pattern of expression ... of six different samples of bone marrow mesenchymal stem cells (three normal, two osteoarthritis and one rheumatoid arthritis) and synovium mesenchymal stem cells (two rheumatoid arthritis, two...

Ngày tải lên: 09/08/2014, 07:20