wharton s jelly mesenchymal stem cell

... J Med Sci 2011, ligands expressed in injured tissues play important roles (2) Stem cells circulate among tissues, and stem cells migrate to the injured tissues once injury occurs Stem cell homing ... deviation (SD) Statistical analysis was performed with SAS6.12 statistic software package and Student t test was carried out for comparisons A value of P

Ngày tải lên: 25/10/2012, 11:18

... immunosorbent assay (ELISA) analysis To determine the reason for this lack of IL-1b and TNF-a secretion by MSCs, changes in these factors’ translation in hypoxia ⁄ SD-stimulated MSCs were investigated ... results of ELISA analysis of supernatants from MSCs after hypoxia ⁄ SD stimulation for 12 h in the presence and absence of ATP and LPS (B) Representative western blots for TNF-a expression in MSCs ... and TNFa transcriptional upregulation However, these two factors are not secreted from hypoxia ⁄ SD-stimulated MSCs unless a second signalling stimulus is present This finding suggests that the...

Ngày tải lên: 18/02/2014, 04:20

... metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING ... of Sciences, Hue University Ministry of Health and the Ministry of Higher Education and Research, The Paris VI University Abstract This paper presents some characteristics of mesenchymal stem cells ... at tumors in mice injected with mysenchymal stem cells at the doses of 107cells/ml is statistically less than that of the control group (p

Ngày tải lên: 20/03/2014, 09:21

... test systems Although primary human hepatocytes are the best source of cells for such systems, their use for this purpose is limited Transcriptome in hepatic induction of AT-MSCs by donor shortage ... determining stem cell transdifferentiation Discussion AT-MSCs may be good candidates as stem cells for cell transplantation and tissue engineering in regenerative medicine, as a large number of AT-MSCs ... neurogenic mesenchymal stem cells in human scalp tissue Stem Cells 23, 1012–1020 In’t Anker PS, Scherjon SA, Kleijburg-van der Keur C, de Groot-Swings GM, Claas FH, Fibbe WE & Kanhai HH (2004) Isolation...

Ngày tải lên: 30/03/2014, 04:20

... and spleen cells Cell Tissue Kinet 1970, 3:393-403 da Silva Meirelles L, Chagastelles PC, Nardi NB: Mesenchymal stem cells reside in virtually all post-natal organs and tissues J Cell Sci 2006, ... fragments that are usually discarded in surgical procedures may represent important sources of stem cells and their use does not pose ethical problems This is the first study to demonstrate the isolation, ... analysis of freshly digested and not cultured hFT, where we used mesenchymal stem cells markers (CD13, CD29, CD44, CD73, CD90, Stro-1, SH2, SH3 and SH4), as well as tissue specific markers (CD14,...

Ngày tải lên: 18/06/2014, 15:20

... of stroke has seldom been discussed [19] Compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, ADMSCs have the distinct advantages of being abundant, easy to obtain ... excised Then 200-300 μL of sterile saline was added to every 0.5 g of tissue to prevent dehydration The tissue was cut into < mm3 size pieces using a sharp, sterile surgical scissors Sterile saline ... multiple-comparison post hoc test Statistical analyses were performed using SAS statistical software for Windows version 8.2 (SAS institute, Cary, Page of 16 NC) A probability value < 0.05 was considered statistically...

Ngày tải lên: 18/06/2014, 16:20

... tissue As compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy ... cut into < mm3 size pieces using a pair of sharp, sterile surgical scissors Sterile saline (37°C) was added to the homogenized adipose tissue in a ratio of 3:1 (saline: adipose tissue), followed ... multiple comparison procedure SAS statistical software for Windows version 8.2 was utilized (SAS institute, Cary, NC) A probability value

Ngày tải lên: 18/06/2014, 16:20

... multiple-comparison post hoc test Statistical analysis was performed using SAS statistical software for Windows version 8.2 (SAS institute, Cary, NC) A probability value

Ngày tải lên: 18/06/2014, 19:20

... transduction was analyzed by FACS: (E) Control MSCs, (F) GFP-MSCs, (G) SVV/GFP-MSCs (C-D): SVV expression in gene modified MSCs, (C): no SVV expression in GFP-MSCs, (D): stronger SVV expression ... passes, and then used for transplantation The efficiency of gene transduction was assessed with FACS SVV expression in modified MSCs The survivin expression was detected by immunofluorescence staining ... bottle was covered with long-spindle cells Passaged cells (mostly spindle cells) were uniformly distributed, and covered the bottom every ~ days The 3rd Passage MSCs highly expressed the surface...

Ngày tải lên: 18/06/2014, 19:20

... expressed as means ± SD Statistical analysis was adequately performed by ANOVA followed by Bonferroni multiple-comparison post hoc test Statistical analysis was performed using SAS statistical software ... marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated ... Windows version 8.2 (SAS institute, Cary, NC) A probability value

Ngày tải lên: 18/06/2014, 22:20

... MannWhitney test using SPSS software 13.0 (SPSS Inc., Chicago, IL, USA) The results are expressed as the mean value ± standard of deviation Statistical significance was set at P < 0.05 Tissue preparation ... close association between the adipogenesis of MSCs and steroid-associated ON development during early stage A close association between elevated adipogenesis of MSCs and steroid-associated osteonecrosis ... adipogenesis of MSCs was associated with steroid-associated ON extension 10 11 12 There were few published works exploring the relationship between adipogenesis of MSCs and steroid-associated ON Lee studied...

Ngày tải lên: 20/06/2014, 00:20

... transduction was analyzed by FACS: (E) Control MSCs, (F) GFP-MSCs, (G) SVV/GFP-MSCs (C-D): SVV expression in gene modified MSCs, (C): no SVV expression in GFP-MSCs, (D): stronger SVV expression ... passes, and then used for transplantation The efficiency of gene transduction was assessed with FACS SVV expression in modified MSCs The survivin expression was detected by immunofluorescence staining ... bottle was covered with long-spindle cells Passaged cells (mostly spindle cells) were uniformly distributed, and covered the bottom every ~ days The 3rd Passage MSCs highly expressed the surface...

Ngày tải lên: 20/06/2014, 04:20

... of cells present on tumor surfaces Luciferase expression in tissue lysates Tumors and selected organs (liver, spleen, intestine, kidney) after imaging were used to prepare tissue lysates Organs ... MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells ... receptors was also proposed as a means to obtain targeted cell vehicles Much of the groundwork for such targeting approaches has previously been established for immune cells (T-cells, NK cells, CIK cells),...

Ngày tải lên: 20/06/2014, 07:20

... tsomla ,dnoceS ]52,32,5[ 16DC/15DC fo noisserpxe eht dna ytivitca esatahpsohp dica tnatsiser-etartrat gnorts a sa hcus stsalcoetso rof srekram desserpxe sllec esehT sllec ekil -tsalcoetso detaelcunitlum ... sllec fo sepyt owt eseht tub ,erutlucbus eht gnirud stsalcoetso dna sCSM eht neewteb dehcated emoceb ot ecnereffid emit eht si ereht esuaceb stsalcoetso morf sCSM eht etalosi ot deirt ew ,sCSM ... erew sllec ehT sCSM fo noitaitnereffid larueN )ASU ,amgiS( noitulos etartin revlis %2 a htiw dezilausiv saw seludon ])2)HO(6)4OP(01aC([ etitapayxordyh fo ecneserp ehT 41 yad no dessessa saw sisenegoetsO...

Ngày tải lên: 07/08/2014, 18:21

... expressed as the mean ± SD Statistical analysis was performed using one-way ANOVA analysis followed by a posthoc LSD test The acceptance level for statistical significance was p＜0.05 Results Establishment ... manufacturer Statistical analysis Experiments were repeated three times The band from the western blot was scanned and analyzed by densitometry using the Sigma Gel System (SPSS, USA) Density values are ... expression in PC12 cells There are several possibilities for what mediates this Discussion 380 Guang-Zhen Jin et al induction First, PC12 cells can synthesize and release dopamine [8], and MSCs...

Ngày tải lên: 07/08/2014, 20:23

... tnacifingis deredisnoc saw 50.0 < p A AVONA yaw -eno a gnisu dessessa saw snaem puorg gnoma secnereffid eht fo ecnacifingis lacitsitats ehT )ASU ,SSPS( erawtfos 0.21 SSPS gnisu tuo deirrac saw sisylana ... eht retfa skeew ta dezinahtue erew sgod lla ,segnahc lacigolohtapotsih eht ssessa oT :tnemssessa lacigolohtapotsiH yrujni eht retfa skeew dna ,0 ta senalp lattigas W2T ni desserpxe snoisel yrujni ... yrosnesotamos ehT slaitnetop dekove yrosnesotamoS ± naem eht sa desserpxe saw ataD :sisylana lacitsitatS droc lanips degamad eht fo retnecipe eht ta demrofrep erew sesylana owt esehT erawtfos emas eht...

Ngày tải lên: 07/08/2014, 23:22

... granule -cell mixture and invasion of fibrous connective tissues into the implant instead of vascular and host bone ingrowth In assessing the present observations, it was suggested that vascular ... defect Tissue Eng 1997, 3, 173-185 13 Kadiyala S, Young RG, Thiede MA, Bruder SP Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro Cell Transplant ... canine segmental bone defects J Bone Joint Surg Am 1998, 80, 985-996 Chang YJ, Shih DT, Tseng CP, Hsieh TB, Lee DC, Hwang SM Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from...

Ngày tải lên: 07/08/2014, 23:22

... CD44, CD105 Also the cUCB-MSCs strongly expressed the embryo stem cells associated surface marker Table Fluorescence-activated cell sorter analysis of canine UCB -mesenchymal stem cells Marker CD4 ... explains the possibility of stem cells possessing a neural progenitor s characteristics A similarity between various tissues has been observed in previous studies [20,21] We also found the undifferentiated ... Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one very critical issue for stem cell therapy [12,28,33] The purpose of this study was...

Ngày tải lên: 07/08/2014, 23:22