vpr s effects as an adjuvant therapy to cart for hiv
Báo cáo y học: "Antiviral activity of a-helical stapled peptides designed from the HIV-1 capsid dimerization domain" pps
... posttransfection and RTnormalized virus was used for infectivity assays In vitro experiments were performed as follows: 293T cells in 60-mm dishes were transfected as above and the virus supernatant was collected ... (Gag), respectively Virus assembly and release assays and immunoprecipitation analysis For metabolic radiolabeling assays, 293T cells (plated at 2.5 × 10 cells/well in 12-well dishes) were transfected ... core assembly being reversible by VSV-G pseudotyping [75] Anti -HIV- 1 activity and cytotoxicity of stapled peptides in cell-based assays We measured the anti -HIV- 1 activity of NYAD-201 and its analogs...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... electrospray mass spectrometer Esquire 3000+ series (Bruker Daltonics) coupled to an online 1100 series HPLC (Agilent Technologies, Santa Clara, CA, USA) Nebulization was assisted by N2 gas (99.8%) ... Acknowledgements We are grateful to Professor N V Joshi for the analysis of TIM sequences and several illuminating discussions The mass spectral facility was supported under the Proteomics program ... active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources facilitate an analysis of mutational effects Most importantly,...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... Arg75, and the predicted mass of this peptide is thus 6073 Da The measured mass is 363 mass units higher than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides ... full mass spectrum and ETD MS ⁄ MS spectra on the six most abundant ions every s The reaction time with fluoranthene radical anions was set to 30 ms, and PTR was not implemented Three ETD scans (300 ... instrument, ion signals are resolved into 12C and 13C isotope peaks separated by ⁄ Da This indicates that the charge on the ions in both mass windows must be +5 and allows them to be assigned to...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... International) was used as a positive control Median values of fluorescence intensity were taken as the binding intensities As many as 10 000 cells were counted for every sample during acquisition, and each ... cEL and cYT (Table 1) were designed and purchased from Multiple Peptide Systems (San Diego, CA, USA) The pure product was analyzed by HPLC and fast atom bombardment mass spectrometry (FABMS) The ... Coupling constants (3JHNa) were measured from the DQF-COSY spectrum Intensities were assigned as strong, medium and weak with upper and lower boundaries of distance for dNa (i, i), daN (i, i +1) and...
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Báo cáo khóa học: Antimicrobial activities of heparin-binding peptides pdf
... Sweden The purity and molecular mass of these peptides was confirmed by mass spectral analysis (MALDI.TOF Voyager) Microorganisms Enterococcus faecalis 2374, Escherichia coli 37.4, Pseudomonas ... comprising multiples of AKKARA or ARKKAAKA [15,20] were synthesized and tested in bactericidal assays using E faecalis as the test organism, and the results demonstrate that these peptides are also ... these peptides and proteins include growth stimulus and angiogenesis (various growth factors, angiogenins) [12,39], protease inhibition [40], antiangiogenesis (endostatin) [41], chemotaxis (chemokines,...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... plates was sufficient for further analyses Unfortunately, X-ray analyses of such plates were unsuccessful because the diffraction of the crystals decreased very rapidly during the measurements This ... Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 533 (projects A1, A2, A3) The authors wish to thank K.-H Mann for N-terminal amino acid analyses and F Siedler and S Korner for mass spectrometry ... delay was set to s The 90° pulse was determined to be 8.4 ls The spectra were processed by fast Fourier transformation including a Gaussian window function and digital filtering of low frequencies...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... different tissues analysed, penaeidin mRNAs were detected in circulating haemocytes in blood vessels and sinuses and in cells present within most tissues The shape of the positive cells suggests that ... investigate the ability of tissues other than haemocytes to express penaeidins and to study the distribution of both transcripts and peptides in response to challenge, shrimp tissues were analysed ... well as a massive release of penaeidin which spread into muscle tissue around the injection site, as a local antimicrobial response As previously demonstrated, penaeidins are released, upon stimulation,...
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Báo cáo khoa học: Non-hydrolytic functions of acetylcholinesterase The significance of C-terminal peptides pptx
... amplified into a sustained process for neurogenesis Such a system could be valuable in, say, the hippocampus, where adult neurogenesis has been reported as a basis for cognitive prowess [40,41] ... populations are the very nuclei linked to primary vulnerability in the neurodegenerative diseases: Alzheimer s disease (basal forebrain, raphe nuclei, locus coeruleus); Parkinson s disease (substantia ... Grisaru D, Hemo Y, Golan-Hadari D, Grant A, Eldor A & Soreq H (2002) The stress-associated acetylcholinesterase variant AChE-R is expressed in human CD34(+) hematopoietic progenitors and its C-terminal...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... also involve extracellular heparan sulfate and various endocytosis and macropinocytosis pathways [16–23] It is also suggested that classical and nonclassical endocytosis pathways may be associated ... was loaded along with 200 ng of pure plasmid DNA) DNaseI protection assay Tat2–plasmid DNA complex was prepared as described for the gel retardation assay For DNaseI assay, lL of DNaseI (RNase-Free ... were chosen as the system for investigation because they are an important tissue in which to study various biochemical processes during seed development They also serve as a model tissue for genetic...
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Báo cáo khoa học: Zinc potentiates the antibacterial effects of histidine-rich peptides against Enterococcus faecalis pot
... contrast (Nomarski) imaging was used to visualize bacterial cells Nomarski imaging is a modification phase microscopy in which samples are visualized in phase microscopy by producing contrast from ... Gram-positive and Gram-negative bacteria for susceptibility to Zn2+ As shown in Table 2, among the bacteria tested, the Gram-positive E faecalis demonstrated least sensitivity to Zn2+, and was therefore ... concentrations (Fig 1B), as well as using a constant molar excess of Zn2+ to peptide (Fig 1C), is compatible with this hypothesis In contrast to experiments with the AHH peptides, the AKK24 and ARK24...
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Báo cáo khoa học: Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides docx
... SB & Marshall B (2004) Antibacterial resistance worldwide: causes, challenges and responses Nat Med Suppl 10, S1 22 S1 29 Walsh FM & Amyes SGB (2004) Microbiology and drug resistance mechanisms ... debated It has been thought that such amphipathic structures might strongly interact with lipids and self-associate to form pores into the membranes or may disintegrate the membranes in a detergent-like ... membrane-associated peptides has been a major challenge to most biophysical techniques Fortunately, recent NMR studies have shown that atomic-level 3D structure, membrane orientation and dynamics...
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Báo cáo khoa học: Effect of the -Gly-3(S)-hydroxyprolyl-4(R)-hydroxyprolyltripeptide unit on the stability of collagen model peptides ppt
... 3 (S) Hyp degrades much faster than 4(R)Hyp during hydrolysis in m HCl, as used in amino acid analysis [14] In fact, reported contents of 3 (S) Hyp are inconsistent even for the same tissue and species ... Discussion and analyzed by CD at 235 nm with a scanning rate of °CÆh)1 All peptides showed a cooperative transition, and little hysteresis was observed, as the heating and cooling curves almost ... position also have these properties, and therefore a smaller transition enthalpy The change in the transition enthalpy in the series of host-guest 27-residue peptides was greatest for the first...
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Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-affinity thrombopoietin antagonists and agonists pot
... remarkably resistant to serum proteases and membrane-bound proteases of the intestinal mucosa [5], which is also particularly the case for ETTP, which displays a half-life for degradation by membrane-bound ... indicated as sticks and numbered according to their occurrence in the sequence b-Strands are shown as arrows Sequences and disulfide connectivities are indicated below Loops responsible for the ... AF12505 ally similar to AGRP* but displays resistance against soluble and membrane-bound human proteases, a feature that may be useful for oral application [5] As no structural information on...
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Báo cáo khoa học: Bilayer localization of membrane-active peptides studied in biomimetic vesicles by visible and fluorescence spectroscopies pptx
... binding and penetration into lipid bilayers The lipid/PDA assembly was previously shown to organize in biomimetic bilayer domains and the assay has been used for studying diverse membrane processes ... lipid/PDA vesicle assay as a platform for studying membrane processes was based upon the unique biochromatic properties of the mixed vesicles [22,23], allowing their application as a useful tool for ... corresponding m(0) values were determined as described previously [42,44] The m(1) values were assessed by inspection of the reconstructed TRES [42] In all cases, the solvent response cannot be satisfactorily...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... nervous tissue The SHSY5Y human neuroblastoma cell line, used in this study as an in vitro model for human neurones, expressed and processed the hproNPFFA to generate SQA-NPFF, NPFF and NPAF These ... peptides from cells and tissues were extracted and purified by RP-HPLC, assessed by radioimmunoassay and identified by using on-line capillary HPLC/nanospray ion trap tandem mass spectrometry (nanospray ... This could be due, as in COS-7 cells, to the fact that a majority of peptides observed in tissues correspond to a degradation into shorter forms such as NPFF and represents for a great part an...
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mass spectrometry of proteins and peptides
... acquisition, and database searching Materials 2.1 Instruments An LCQ tandem mass spectrometer (Finnigan MAT, San Jose, CA) is used in our laboratory Other tandem mass spectrometers capable of automated ... charge state Tandem mass spectra are searched against databases using the SEQUEST search algorithm The database searching method is described in ref 10 All single tandem mass spectral matches to ... (Fig 2) Since esterification with methanol significantly shifts the masses of y′′ ions (by 14, 28, 42, mass units), it is possible to use low-resolution settings when sequencing is performed...
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Báo cáo toán học: " Measurement of beta amyloid peptides in specific cells using a photo thin-film transistor" pot
... transistor and shows the results of the photocurrents resulted from a fluorescence signal Methods Photosensitive field-effect transistor As shown in Figure 1, we propose an effective method to ... 2d), and the p-FET sensor was imaged using a fluorescent microscope and was irradiated by a 405-nm blue laser as an excitation source for FITC to measure the photocurrent The reason that we used ... and forms plaques [12] As an Aβ detection tool, highly sophisticated neuroimaging techniques have been developed such as single photon emission computed tomography [13] and positronemission tomography...
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Overview of User Interface Design docx
... so on? Task times: pros and cons • Task times can measure many usability factors – It is also the classical way of measuring usability – When we want to buy a system, we can run usability tests ... test tasks is a critical issue, just as for task time measurements 4 Keystroke counts • Keystroke counts can, to some extent, help us specify and measure task efficiency for experienced users – ... tests with it to see how fast our users can learn to perform their tasks – Usually, the system is already used somewhere else, so we might even get access to experienced users and measure how fast...
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Báo cáo y học: "Salivary gland derived peptides as a new class of anti-inflammatory agents: review of preclinical pharmacology of C-terminal peptides of SMR1 protein" pptx
... contributes to difficulties in tasting, eating, swallowing, and speaking, and results in sores of the soft tissues of the mouth and periodontal disease These pathologies also manifest in human patients ... systemic diseases including - Sjögren s syndrome, rheumatoid arthritis, juvenile idiopathic (rheumatoid) arthritis, systemic lupus erythematosus (an inflammatory connective tissue disease), systemic ... such as diabetes, respiratory diseases and osteoporosis [60] Likewise in veterinary medicine epidemiologic studies reveal that oral disease is the most common disease in all age groups of dogs and...
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