vitro+ex+vivo+strain+gauge+analysis

... constructs was measured by firefly luciferase expression and normalized by the Renilla luciferase expression Both pGL3-2.6k and pGL3-2.2k constructs had luciferase expression levels 5fold higher than that ... gambiae renders the mosquito an ability to repress ONNV replication [23,24] These in vivo findings and our current ex vivo characterization of the hsc70B regulatory region unequivocally indicate that ... replicates The largest and the smallest values from these replicates were excluded from the analysis Viruses The SG650 strain of ONNV has previously been described [23] The WNV isolate (LA-11-2005)...

Ngày tải lên: 20/06/2014, 01:20

... the second day while the tumor uptake Table In vitro analysis of panitumumab F(ab’)2 conjugated with CHX-A"-DTPA Chelate:mAb ratio (molar excess) Analysis Panitumumab F(ab’)2 5× 10× 20× IC50 (nM) ... wells In vivo studies Quantitation of tumor targeting All animal care and experimental protocols were approved by the National Cancer Institute Animal Care and Use Committee The in vivo behavior ... determined by imaging and ex vivo quantitation (Figure 5c and Table 4) As a prelude to: (1) utilizing the panitumumab F(ab’)2 for monitoring response to radioimmunotherapy and (2) exploiting the panitumumab...

Ngày tải lên: 21/06/2014, 02:20

... acquisition, analysis and interpretation of data of erythrocyte aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments ... interval (n = 6) Degree of significance of the difference between groups: * p < 0.05 Ex vivo experiments Previous analysis [16] performed on erythrocytes from active RA patients (DAS 28-4 > 2,6) ... Rigidity Index 30 25 20 15 10 0 50 100 150 200 [HA] ( g/ml) Figure Rigidity Index (RI) of erythrocytes incubated in vitro with variable HA concentrations, and reversibility of HA effect In vitro effect...

Ngày tải lên: 10/08/2014, 05:21

... perform subsequent experiments at ≤ 26 μM WR1065 For the Annexin V assay, drug-exposed cultures ranged from 75% to 100% Annexin V-negative (non-apoptotic), with the majority of experiments showing ... WR1065, yielding a therapeutic index of 0.25 for the cell culture studies However, this relatively-poor in vitro therapeutic index is not relevant for the in vivo potential because the parent ... with drug, we assayed for Annexin V (as previously described [22]) Cells taken from the wells used for p24 protein analysis were subjected to flow cytometry for this analysis and Page of 10 (page...

Ngày tải lên: 10/08/2014, 05:21

... cyclooxygenases ex vivo; and (b) inhibit IL-1β-induced production of nitric oxide (NO) and PGE2 by rabbit articular cartilage chondrocytes in vitro Extraction of anthocyanins from blood and HPLC analysis ... evidence from in vitro studies does not prove in vivo biological activity, these provide a rationale and support for the use of pomegranate fruit or its extract to suppress inflammation in vivo However, ... that deserve an explanation and require caution in interpreting the data obtained from in vitro studies One question often raised is whether the concentration of a plant or fruit extract constituent...

Ngày tải lên: 11/08/2014, 08:22

... tissue specimens Percentages of culture period ex vivo Percentages of dead cells in human NSCLC tissue specimens following 16 h culture period ex vivo Viability was also assessed without cultivation ... was to examine the reliability of a novel ex vivo tissue model (STST) and to evaluate multiple aspects of human lung tumor behavior in response to conventional chemotherapy With such an experimental ... of the vitality of these tissue samples is crucial for ex vivo cultivation As a consequence of extensive testing the culture period did not exceed 16 h, limiting also the chemotherapeutical treatment...

Ngày tải lên: 12/08/2014, 15:20

... to switch templates both in vivo and ex vivo [1620] While template switches of retroviral RT's are critical for retrovirus biology, RT template switches occuring ex vivo and thus producing sequence ... tissue sample Recombinations are very likely due to ex vivo recombination There are two opportunities for ex vivo recombination in the course of the experiment First, during cDNA generation, reverse ... a combination of both mechanisms In this context, we were able to produce 10.5% of recombinant gag sequences in a separate ex vivo experiment by in vitro transcribing different HML-2 gag sequences,...

Ngày tải lên: 13/08/2014, 05:22

... that these modifications are physiologically relevant because: (a) they occur ex vivo in human liver cell lines and in vivo in human plasma; (b) in the case of the propeptide, phosphorylation is ... sulfation at Tyr38 [4,5] Using the same technique, we next examined propeptide phosphorylation from the media of several cell lines transfected with the expression vector for a C-terminal V5-tagged wild-type ... transfected with a hPCSK9 expression vector were collected and immunoprecipitated as above for TOF-MS analysis of immuno- 3490 captured PCSK9 as previously described, except that following immunoprecipitation,...

Ngày tải lên: 16/03/2014, 06:20

... related expansion of CD4+/CD25+/ CD127-/FoxP3+ regulatory T cells IL-2 is critical for the ex- vivo expansion of suppressive regulatory cells [25] Using high doses of IL-2 in our NK cell expansion ... regulate the proliferation and the expansion of T cells was maintained in the presence or absence of exogenous IL-2 (Fig 3b) Thus, monocytes are involved in the ex- vivo priming of autologous CD4+T ... cells in the context of SCT also induces potent antitumor effects [9,11] and such therapeutic approaches are now realistic in humans [12] More generally, adoptive transfer of ex- vivo expanded NK cells...

Ngày tải lên: 18/06/2014, 15:20

... of 1:6 The QD density was *4 108 cmÀ2 In order to estimate the strain state of individual QDs, we ´ have carried out a detailed analysis of the moire fringe pattern seen in planar view TEM images ... the residual strain, f is the lattice mismatch and dInN is the natural distance between f1" g planes of InN 100 By simple substitution, we obtain d = 97 ± 6%, i.e essentially complete strain relief ... quantitative strain maps with nm resolution [14, 15] Working on the Fourier transform of the HRTEM image, we applied separate Bragg masks around the 1" 100 peaks of both InN and GaN, excluding the...

Ngày tải lên: 22/06/2014, 18:20

... sàng l c tác d ng b o v gan ex vivo Tác d ng b o v gan c a cao methanol Râu mèo ñư c trình bày hình 250 Ho t ñ ALT (U/L) 200 150 100 50 Nhóm Hình Ho t tính b o v gan exvivo c a cao chi t Râu mèo ... p, s d ng m t lư ng m u nh Các th nghi m ex vivo có ưu ñi m g n v i h th ng sinh h c nên thư ng ñư c dùng nh ng th nghi m sàng l c trư c th nghi m in vivo Trong th nghi m t bào gan tách r i, ... gian 90 phút bi n mô hình th nghi m gây t n thương ñư c trình bày hình K t qu cho th y gan ex vivo in vivo Ch t gây nên s xơ n ng ñ 1,0 1,5%, lư ng enzym gan ti t hóa gan làm thay ñ i ch s sinh...

Ngày tải lên: 27/06/2014, 10:20

... sàng l c tác d ng b o v gan ex vivo Tác d ng b o v gan c a cao methanol Râu mèo ñư c trình bày hình 250 Ho t ñ ALT (U/L) 200 150 100 50 Nhóm Hình Ho t tính b o v gan exvivo c a cao chi t Râu mèo ... p, s d ng m t lư ng m u nh Các th nghi m ex vivo có ưu ñi m g n v i h th ng sinh h c nên thư ng ñư c dùng nh ng th nghi m sàng l c trư c th nghi m in vivo Trong th nghi m t bào gan tách r i, ... gian 90 phút bi n mô hình th nghi m gây t n thương ñư c trình bày hình K t qu cho th y gan ex vivo in vivo Ch t gây nên s xơ n ng ñ 1,0 1,5%, lư ng enzym gan ti t hóa gan làm thay ñ i ch s sinh...

Ngày tải lên: 27/06/2014, 11:20

... three surgeons Statistical analysis: Fisher's exact test was used for the data analysis A level of P < 0.05 was considered to indicate statistical significance RESULTS Ex vivo hepatic venography ... age at LDLT of 59 years (range; 52-68 years) (Additional file 1, Table S1) Ex vivo hepatic venography: Using livers explanted for LDLT, hepatic venography was performed with contrast media (Urografin, ... Ex vivo hepatic venography for hepatocellular carcinoma in livers explanted for liver transplantation Kensuke Miyazaki, Akihiko Soyama,...

Ngày tải lên: 09/08/2014, 02:21

... Identification of Th2-type suppressor T cells among in vivo expanded ocular T cells in mice with experimental autoimmune uveoretinitis Clin Exp Immunol 2001, 124:1-8 57 Gao Q, Rouse TM, Kazmerzak ... peripheral T cells have immune suppressive activity in vitro Eur J Immunol 2001, 31:1247-1254 Dieckmann D, Plottner H, Berchtold S, Berger T, Schuler G: Ex vivo isolation and characterization of CD4+CD25+ ... regulatory cells suppress naive and memory T cell proliferation and can be expanded in vitro without loss of function J Exp Med 2001, 193:1295-1302 16 Jonuleit H, Schmitt E, Stassen M, Tuettenberg...

Ngày tải lên: 09/08/2014, 03:24

... collection, selection and in -vitro maturation culture of mammalian gametes used for in -vitro fertilization - Establish protocols for In -vitro fertilization , embryo culture, embryo sexdetermining (PCR ... chuyển phôi in -vitro, in -vivo chuột nhắt trắng Đông lạnh phôi chuột kỹ thuật đông lạnh nhanh chậm Nitơ lỏng Quy trình thu nhận, tinh nuôi cấy tế bào mầm từ nguồn phôi chuột in vivo Ý nghĩa khoa ... thụ tinh in- vitro chuột nhắt trắng heo Quy trình xác định giới tính phôi giai đoạn sớm heo kỹ thuật sinh học phân tử Thiết lập quy trình nuôi cấy nguồn phôi chuột in -vitro, in -vivo đến giai...

Ngày tải lên: 10/08/2014, 04:21

... day-14 ex vivo expansion cultures: Liquid cultures for 14 day ex vivo expansion were established with 100,000 CD34+ cells in 50 ng/ml of SCF, TPO and Flt-3 Ligand The results of six separate experiments ... in clinical-scale transplantation using ex vivo CB products To overcome this problem, new approaches for ex vivo expansion need to be developed, aiming at expansion of the "stem" cell population ... was encountered in some earlier attempts using ex vivo expanded CB products, despite of satisfactory and impressive results from preclinical ex vivo experiments [16-18] Stiff and colleagues have...

Ngày tải lên: 10/08/2014, 22:20

... effector cells after ex vivo expansion Phenotype Phenotype analyses of IFN-γ- or CD137-selected immune effector cells after ex vivo expansion (A) Phenotype analysis after ex vivo expansion After the ... cells after ex vivo expansion Effector function analyses of IFN-γ- or CD137-selected immune effector cells after ex vivo expansion (A) and (B) Flow cytometry analysis of IFN-γ and CD137 expression ... 2009, 2:34 http://www.jhoonline.org/content/2/1/34 Figureexpansion and phenotype analyses of DC-activated immune effector cells Ex vivo Ex vivo expansion and phenotype analyses of DC-activated immune...

Ngày tải lên: 10/08/2014, 22:20

... Ex vivo effects of flavonoùds extracted from Artemisia herba alba on cytokines and nitric oxide production in Algerian ... NaNO2 standard (0-200 àmol/mL) curve Results were expressed as àM of nitrites in supernatants of PBMC cultures Statistical analysis Results were expressed as the mean standard deviation Statistical ... interests Authors' contributions MD carried out the experimental work, collected and interpreted the data BH and ML carried out most of the in vivo experiments TM and OF recruited the ABD patients...

Ngày tải lên: 11/08/2014, 03:20

... patients, using an ex vivo model of Gram negative Lipopolysaccharide (LPS) induced acute inflammation We found that COPD disease severity was associated with an enhanced ex vivo pro-inflammatory ... pg/mg of tis- In this study, we have employed an ex vivo lung explant model to investigate the initial acute inflammatory response initiated by exposure to Gram negative bacterial cell wall component ... networks involved in an exacerbation have remained unexplored The majority of clinical studies have previously concentrated on examining the acute inflammatory response during exacerbations of COPD...

Ngày tải lên: 12/08/2014, 15:21