visualization of cellulose synthase demonstrates functional association with microtubules 1491

... at the beginning of day and those at the end of day (p = 0.006) 24.2 ± 4.5 Years of employment (mean ± SD) Page of 13 Characteristics of the studied male workers smokers None of the workers was ... Measurement of 1-OHP in urine The analysis of 1-OHP, a metabolite of pyrene, is proposed as a reliable biomarker of the internal dose for PAH exposure [32] However, it is not representative of genotoxic ... correlated with the within-shift variation of the reducing species (Spearman r = 0.59, p < 0.0001; Figure 2(b)) The range of variation for reducing species (-80% to +1000%) was much greater than that of...

Ngày tải lên: 20/06/2014, 00:20

... Heart Study Offspring cohort included surviving descendants (and spouses of descendants) of the original Framingham Heart Study cohort [25] As part of a study of the inheritance of OA, selected ... incident cases of hand or knee OA as compared with women without OA Of the markers we studied, NTx appears to exhibit the greatest discrimination between participants with and those without BMLs ... significant association with BMLs, with the odds of a BML being present increasing by 1.4-fold (95% confidence interval = 1.0-fold to 2.0-fold) per standard deviation increase in the LnNTx, and with...

Ngày tải lên: 09/08/2014, 13:21

... the cutoff frequency to 12 Hz, and the time constant to 0.2 seconds A constant back-scattered light of at least 30% of the emitted light indicates an adequate contact of the optical probe with ... evidence of a potentiation of vasomotion by hyperaemia During midazolam infusion, an inhibition of this increase of vasomotion induced by reactive hyperaemia was noted Frequency analysis of the ... messages • Sedation with midazolam alone or in association with sufentanil induces a deterioration of vasomotion and microvascular response to ischaemia This raises the question of whether sedation...

Ngày tải lên: 13/08/2014, 03:20

...

Ngày tải lên: 14/08/2014, 08:21

... 25 1.5 HYPOTHESIS CD44 is a molecule with a spectrum of functional effects It has been used as a marker for the identification of a subpopulation of cells with tumourigenic properties in head ... immunohistochemical expression of CD44 may be indicative of the proportion of cancer stem cells within the tumour It may therefore have prognostic significance in the clinical outcomes of patients with oral tongue ... assess association of factors with each endpoint The median follow-up since surgery was 4.2 years Intensity of CD44s staining and percentage staining varied among the samples Intensity of staining...

Ngày tải lên: 02/10/2015, 17:14

... effect of EGFP–Ki-1 ⁄ 57 in modifying the pattern of splicing of E1A mRNA in comparison with empty pEGFP vector (Fig 4B) Expression of EGFP– Ki-1 ⁄ 57 leads to formation of the 10S and 9S isoforms, ... concomitantly with a reduction of 13S isoform formation, in a dose-dependent way (Fig 4B, lanes 2–4) This finding strongly suggests the functional involvement of Ki-1 ⁄ 57 in regulatory mechanisms of pre-mRNA ... colocalization of Ki-1 ⁄ 57 with SMN ⁄ GEMS in Adox-treated cells (Fig 7Biii) Despite the diffuse nuclear distribution of EGFP–Ki-1 ⁄ 57, the majority of the red GEMS spots coincide with spots of brighter...

Ngày tải lên: 16/03/2014, 02:20

... DSTPLAN4.2 software, in order to assess the probability of detecting an association between RANTES polymorphisms and asthma at the 0.05 level of significance, assuming a genotypic risk of 2.0 and ... GSTP1 Ile105Val polymorphisms may functionally related to the risk of esophageal cancer A number of molecular epidemiology studies have been conducted to examine the association between p53 Arg72Pro, ... reached a consensus on all of the items Data extracted from these articles included the first author’s name, year of publication, country of origin, ethnicity, number of cases and controls, genotype...

Ngày tải lên: 25/10/2012, 11:40

... along with appropriate family screening In 2530% of cases, this might provide the first indicator of an underlying FAP syndrome Conflict of interest: The authors have declared that no conflict of ... mutation of the APC gene in a case of cribriformmorular variant of papillary thyroid carcinoma Am J Clin Pathol, 2001 115: 486-493 Yamashita T., et al Peculiar nuclear clearing composed of microfilaments ... tumors [4] Recently Xu B [5] suggested that accumulation of mutant β-catenin contributes to the development of C-MV of PTC A handful of sporadic cases have also been documented [6] Table summarizes...

Ngày tải lên: 03/11/2012, 10:05

... of the increasing mobility of the population, the changing environment, and the biology of bacilli, the prevalence of TB is higher in China Epidemiological studies indicate that only to 10% of ... carried out with 10 Genetics and Molecular Research 11 (2) 1370-1378 (2012) ©FUNPEC-RP www.funpecrp.com.br Association of KIR with pulmonary tuberculosis 1373 cycles at a melting temperature of 94°C ... the results of a sputum smear test (positive or negative) The average age of the subgroups was the same Blood was collected before the initiation of anti-tuberculosis therapy A total of 110 unrelated...

Ngày tải lên: 15/02/2014, 12:20

... on Ser172 of H1.4 For H1.2 and H1.3, the work of Wisniewski et al [43] agrees with that of Garcia et al [42], noting that in Table I of the former paper the phosphorylation on Thr173 of H1.2 is ... phosphorylation state of the linker histones using MS It was found that treatment with all of these compounds reduced the global level of phosphorylation of the H1.2 and H1.4 isoforms Although the ... number of cells in mitosis In addition, in MS experiments, absence of a condition is not proof of its nonexistence Whereas, initially, it was found that PTMs in the N-terminal tail of most of the...

Ngày tải lên: 18/02/2014, 08:20

... the expression of more than one FLS in C unshiu Polypeptide alignments of FLSs with plant 2-oxoglutarate-dependent dioxygenases of different functionality (FHT, anthocyanidin synthase, gibberellin ... 486/33 PC and controlled by Jasco software The spectropolarimeter was equipped with a cylindrical quartz cuvette with a pathlength of 0.05 cm The temperature of the cell holder was maintained ... base searches and sequence alignments were carried out with the ENTREZ and BLAST software (National Library of Medicine and National Institute of Health, Bethesda, MD, USA) The PROSIS package (Hitachi,...

Ngày tải lên: 21/02/2014, 03:20

... failed association of Pex15p with the importomer is not yet clear Most interestingly, in contrast with the lack of other components of the peroxisomal protein import machinery, the deficiency of Pex4p ... membrane Association of the importomer and the AAA complex in import mutants To determine whether the absence of Pex1p, Pex6p or Pex15p has an influence on the association of the AAA complex with ... nor Pex6p influenced the composition of the complex obtained with Pex15p-TEV-ProtA (Fig 4) Thus, we conclude that the association of Pex15p with the components of the import machinery does not depend...

Ngày tải lên: 07/03/2014, 12:20

... fibre cellulose synthase and its inhibiting effect on glucan synthesis together with a recently discovered redox-dependent dimerization of the chain elongation enzymes of cellulose synthase strongly ... regulation of callose synthase Plant J 3, 763–772 11 Kurek, I., Kawagoe, Y., Jacob-Wilk, D., Doblin, M & Delmer, D (2002) Dimerization of cotton fiber cellulose synthase catalytic Ó FEBS 2003 2564 A Hofmann ... before with Anx24(Ca32), the globular structure of Anx(Gh1), unlike that of mammalian annexins, clearly shows separation of the two modules (I/IV and II/III) leading to greater accessibility of the...

Ngày tải lên: 17/03/2014, 03:20

... properties of paper The development of papermaking additives and the accumulation of practical experience of their use, combined with profound understanding of their action mechanisms, along with modern ... II, cellulose III, and cellulose IV, that are not native forms of cellulose but formed upon chemical processing Cellulose III and IV are mainly of scientific interest, but cellulose II is of technical ... biosynthesis of wood (Somerville et al 2004) Utilization of the specific non-electrostatic interactions of polymers with cellulose has generated novel methods of surface modification of cellulose...

Ngày tải lên: 18/03/2014, 02:20

... twice for h at °C with 100 lL of protein G-Sepharose (Sigma, Saint Louis, MO, USA) The samples were then incubated overnight at °C with 10 lg of the relevant mAbs and 100 lL of fresh protein G-Sepharose ... invert) equipped with a Planapo oil immersion lens Results Transfection of GPV in the absence of GPIb–IX results in its inefficient cell surface exposure and release of a soluble form of GPV The CHO/GPV ... transfected with full length GPV lacked surface expression of GPV as measured by flow cytometry (Fig 1A), despite selection of a strongly expressing clone by a series of amplifications in the presence of...

Ngày tải lên: 23/03/2014, 13:20

... at 42.5 2.8 kDa A 1-L culture of E coli yielded mg of pure GAPDH with a specic activity of 146 11 Uặmg)1 when NADPH was used as cofactor and a specic activity of 35 Uặmg)1 when NADH-dependent ... as the A4 GAPDH of C reinhardtii is not activated by BPGA [32] Fig Kinetic changes of recombinant GAPDH upon association with CP12 GAPDH was incubated with CP12 in a molar ratio of : (0.2 nmol ... show that upon association of CP12 with GAPDH, the kinetic parameters of the latter change in a two-step process to nally become identical to those of native GAPDH The decrease of the catalytic...

Ngày tải lên: 23/03/2014, 20:22

... spectra of H1–47 in the absence (red) and presence (black) of 1.5 equivalents of unlabelled E1–100 (A) Overlay of 2D 1H–15N-HSQC spectra of H1–47 in the absence (red) and presence (black) of 1.5 ... Previous fitting of the X-ray coordinates of the atomic models of subunit A from P horikoshii [17] and subunit B of Methanosarcina mazei Go1 [18] into the ¨ electron density map of the A-ATP synthase ... forms one of the two peripheral stalks of the enzyme, with its C-terminus connected to the N-terminal part of the catalytic A subunit and its N-terminus in close contact with the N-terminus of subunit...

Ngày tải lên: 30/03/2014, 04:20

... solubilized with NaOH and the radioactivity was counted Nonspecific binding was measured as the binding of 125I-labelled toxin in the presence of an excess of unlabeled LT-B To assay nonspecific binding of ... SB (2005) Blood group, immunity, and risk of infection with Vibrio cholerae in an area of endemicity Infect Immun 73, 7422–7427 Interaction of LT-I with differentiated HT29 cells 39 Black RE, ... for 18 days were incubated with different concentrations of CT-B or LT-B for 30 at °C and then further incubated with 125I-labeled LT-I (5.0 nM) for 60 at °C Binding of 125Ilabelled LT-I was determined...

Ngày tải lên: 30/03/2014, 10:20

Ngày tải lên: 05/08/2014, 10:20

... significantly associated with decreased functional status and worse health status when compared to those of patients without psychological symptoms, even after controlling for the effects of overall health ... the emotional moiety of the soul); thus it literally implies a deflection of emotions In general, subjects with alexithymia usually complain of somatic symptoms, regardless of their somatic ailment ... prevalence of alexithymia and its association with anxiety and depression in a sample of Greek COPD outpatients Page of Methods Sample From the outpatients list of scheduled appointments at our...

Ngày tải lên: 08/08/2014, 23:21