virus scanners by platform
Báo cáo sinh học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps" pptx
Ngày tải lên :
18/06/2014, 18:20
... vaccinia viruses, alpha-viruses or phages have been developed The latter are also used for generation of recombinant vesicular stomatitis virus (VSV) [6,7] and respiratory syncytial virus (RSV) [8] by ... in nature or be targeted by innate viral defence mechanisms The precise reason why genes of RNA viruses are inefficiently expressed is still poorly understood For lentiviruses, which were studied ... 4:51 genomic RNA Therefore, these viruses are not adapted to the complex nuclear milieu of the eukaryotic host cell Inefficient expression of genes from RNA viruses by RNA polymerase II (Pol II)...
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Báo cáo hóa học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps" ppt
Ngày tải lên :
20/06/2014, 01:20
... vaccinia viruses, alpha-viruses or phages have been developed The latter are also used for generation of recombinant vesicular stomatitis virus (VSV) [6,7] and respiratory syncytial virus (RSV) [8] by ... in nature or be targeted by innate viral defence mechanisms The precise reason why genes of RNA viruses are inefficiently expressed is still poorly understood For lentiviruses, which were studied ... 4:51 genomic RNA Therefore, these viruses are not adapted to the complex nuclear milieu of the eukaryotic host cell Inefficient expression of genes from RNA viruses by RNA polymerase II (Pol II)...
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Báo cáo hóa học: " In vitro inhibition of human influenza A virus replication by chloroquine" pot
Ngày tải lên :
20/06/2014, 01:20
... monolayer and viewed under an ultraviolet light microscope Controls consisting of virus and cell only, cell only, virus only and serial 2-fold dilution of amantadine in place of chloroquine were ... has a clinically useful inhibitory activity against influenza A virus replication The IC50 and IC90 for both the H1N1 and H3N2 viruses are lower than the plasma concentration of chloroquine that ... malaria [12] This suggests that the dosages needed to inhibit human influenza A virus replication should be well tolerated by patients An added effect of chloroquine is its immunomodulatory activity...
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Báo cáo hóa học: "Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay" pptx
Ngày tải lên :
21/06/2014, 19:20
... and other honeybee viruses (deformed wing virus (DWV), chronic bee paralysis virus (CBPV), Kashmir bee virus (KBV)) All SBV strains were positive while all other honeybee viruses were negative ... a color change brought on by the addition of nucleic acid stain SYBR Green Conclusions The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity ... high sensitivity and analytic specificity Keywords Sacbrood virus, Loop-mediated isothermal amplification, SYBR Green, Honeybee Sacbrood virus (SBV) primarily affects honeybee broods, and results...
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Báo cáo khoa hoc:" Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry" pdf
Ngày tải lên :
11/08/2014, 08:20
... of the 1000-fold diluted H5N2 virus lysate (~107.8 EID50/mL, Fig 5B) As low as 0.1 µL of virus lysate, corresponding to ~103.8 EID50 of H5N2 viruses, was detected by SDS-PAGE using silver staining ... scores of the eight matched sequences Figure Virus screening by incubation of AbH5N2@Fe3O4 MNP with the lysate of different viruses The examined viruses (40 µL) include H5N2 (A/Duck/Taiwan/3233/04) ... glycoproteins on the surface of influenza virus with critical roles in virus infection and transmission To date, 16 HA (H1–H16) and NA (N1–N9) subtypes in influenza A viruses have been isolated from avian...
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Báo cáo y học: "Profiling of cellular proteins in porcine reproductive and respiratory syndrome virus virions by proteomics analysis" pps
Ngày tải lên :
12/08/2014, 01:22
... HCMV, HIV, influenza virus particles, and herpes simplex virus [18,20,44,45] HCMV-associated annexin A2 contributes to cell penetration by the virus, and is acquired during virus egress from infected ... human immunodeficiency virus (HIV), emiliania huxleyi virus 86 (EhV-86) virions, kaposi’s sarcoma-associated herpesvirus (KSHV) and influenza virus, that shows the presence of lots of cellular proteins ... PRRSV envelope By doing so, the microvesicles become lighter than the virions and virions can be isolated by density centrifugation Proteins present inside the virion are protected by lipid envelope...
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Báo cáo y học: "Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon a" pptx
Ngày tải lên :
12/08/2014, 01:22
... transcription by HBc protein influences the inhibition of HBV replication by IFN-a Materials and methods Plasmid constructs The parental plasmid for pU19-1.24HBV was kindly provided by Dr Mizokami ... detection of HBc protein expression by western blot as described below, and used for extraction of total RNA by Trizol (Invitrogen) Levels of MxA mRNA were assessed by real-time PCR as described below ... HBV replication by IFN-a In order to evaluate whether the inhibition of MxA transcription by HBc protein could decrease the 2.1 IFN A inhibitory effect on HBV replication induced by IFN-a, pU19-1.24HBV...
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Báo cáo khoa học: " Inhibition of Monkeypox virus replication by RNA interference" pdf
Ngày tải lên :
12/08/2014, 04:20
... Inhibition of virus replication by RNA interference J Biomed Sci 2003, 10:607-616 Broyles SS: Vaccinia virus transcription J Gen Virol 2003, 84:2293-2303 Hsiao JC, Chung CS, Chang W: Vaccinia virus envelope ... methods Viruses and cultures Wild-type monkeypox virus, strain Katako Kombe (MPVKK) and MPV expressing green fluorescent protein (MPVGFP) were used to evaluate siRNA efficacy [19] The viruses ... viral infection Cells infection with virus To infect siRNA transfected and non transfected control cells with MPV, incubation medium was replaced by the virus inoculum diluted to produce the...
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Báo cáo y học: "Control of mucosal virus infection by influenza nucleoprotein-specific CD8+ cytotoxic T lymphocytes" ppsx
Ngày tải lên :
12/08/2014, 15:20
... Influenza viruses Virulent challenge pools of mouse-adapted influenza viruses A/Hong Kong/1/68 (A/H3N2) and A/Taiwan/1/86 (A/H1N1) viruses were prepared by serially passaging each virus in mice ... Mice were infected with live virus by administering 0.05 LD50 A/Taiwan/86 by small particle aerosolization [19,18,20] For DNA vaccination, mice were anesthetized by subcutaneous injection of ... challenged by intranasal inoculation with 10 μl containing 100–200 MID50 of A/HK/68 (H3N2) virus Lung and nasopharyngeal virus titers were assessed days later Quantitation of lung and nasopharyngeal virus...
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Báo cáo sinh học: "Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery" pdf
Ngày tải lên :
14/08/2014, 19:22
... arthropod-borne virus transmission by mosquitoes Clin Microbiol Rev 2000, 13:651-661 Adelman ZN, Blair CD, Carlson JO, Beaty BJ, Olson KE: Sindbis virus- induced silencing of dengue viruses in mosquitoes ... After days the cells were infected with DEN-2 virus at an MOI of 0.1 Five days later, the numbers of DEN-2 virus infected cells were quantified by flow cytometry using anti-DEN-envelope protein ... and after days the cells were infected with DEN-2 virus (MOI of 0.1) Five days later, the numbers of DEN-2 virus infected cells were measured by flow cytometry titer compared to control (Fig 3B)...
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Structure of hibiscus latent singapore virus determined by x ray fiber diffraction
Ngày tải lên :
10/09/2015, 15:53
... plant viruses 14 iii 1.6.2 Tobamovirus structure determination by fiber diffraction 16 1.6.3 Other filamentous virus structure by fiber diffraction 17 CHAPTER HIBISCUS LATENT SINGAPORE VIRUS ... Filamentous plant viruses Filamentous plant viruses make up almost half of the plant virus genera The Potyvirus genus alone has been described as including almost a third of known plant viruses (Riechmann ... filamentous viruses (Oda et al., 1998; Stubbs et al., 2000; Yamashita et al., 1998b) 1.6.2 Tobamovirus structure determination by fiber diffraction TMV, a rod shaped virus of the genus Tobamovirus,...
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Studies of shrimp white spot syndrome virus by protein array platform and proteomics approaches
Ngày tải lên :
11/09/2015, 16:06
... WSSV by IEM 99 Figure 5.7 Localization of wsv432 in WSSV by IEM 100 Figure 5.8 Pull down of VP24 by wsv010 103 Figure 5.9 A summary of WSSV structural proteins identified by ... ectodermal and mesodermal baculovirus (SEMBV), rod-shaped virus of Penaeus japonicus (RV-PJ), white spot baculovirus (WSBV) and hypodermal and hematopoietic necrosis baculo-like virus (HHNBV) (Wang, Poulos, ... Scale Cultured and Purified Recombinant WSSV Proteins 61 Study of White Spot Syndrome Virus by Protein Array Platform 65 4.1 Introduction 66 4.2 Material and Methods 66...
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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
Ngày tải lên :
18/02/2014, 16:20
... created by eliminating genome-length HCV RNA from the O cells by IFN treatment; and OR6c, cured cells which were created by eliminating genome-length HCV RNA from the cloned OR6 cells [44] by IFN ... Differential activation of interferon-inducible genes by hepatitis C virus core protein mediated by the interferon stimulated response element Virus Res 97, 17–30 Benech P, Vigneron M, Peretz D, ... its activation is mediated by the TLR3 but not the TLR4 signaling pathway, we examined whether or not activation of IRF-3 by M-pIC treatment is specifically mediated by the TLR3 signaling pathway...
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Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx
Ngày tải lên :
07/03/2014, 11:20
... Ribonuclease zymogen activation by NS3 protease R J Johnson et al Hepatitis C virus (HCV) [18,19], a positive-stranded RNA virus of the family Flaviviridae [20,21], is estimated ... activation by NS3 protease Fig Activation of 1C zymogen by the NS4A NS3 protease Activation at 37 C was monitored at different times after the addition of 0.5 molar equivalents of NS4A NS3 protease by ... RNase A zymogen that is activated by the NS3 protease of HCV The linker of our RNase A zymogen inhibits its activity by a mechanism similar to proteolytic zymogens, by sterically blocking substrate...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
Ngày tải lên :
16/03/2014, 01:20
... (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA 300 SOD2 protein level by Western blot (Arbitrary units) ... (Arbitrary units) B BetA * * CTL CREB protein level by Western blot * SOD2 mRNA level by qPCR (Arbitrary units) 150 (Arbitrary units) qPCR on SOD2 promoter by CREB ChIP A * 100 *¶ CTL BetA BetA/CREB siCREB ... and that this effect was abolished by CREB overexpression in the presence of BetA, but was mimicked by siCREB This indicates that SOD2 expression is regulated by CREB phosphorylation at Ser133...
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Báo cáo khoa học: Effects on protease inhibition by modifying of helicase residues in hepatitis C virus nonstructural protein 3 pot
Ngày tải lên :
16/03/2014, 06:20
... PY, Kao C & Luo G (2005) Stimulation of hepatitis C virus (HCV) nonstructural protein (NS3) helicase activity by the NS3 protease domain and by HCV RNA-dependent RNA polymerase J Virol 79, 86878697 ... hepatitis C virus NS3 protease are obtained by optimizing the cleavage products Biochemistry 37, 89068914 19 Morrison JF (1969) Kinetics of the reversible inhibition of enzyme-catalysed reactions by tight-binding ... found to be more diverse for the less potent compounds The inhibition by compounds and was not affected to any large extent by any of the substitutions, whereas compounds and became more effective...
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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
Ngày tải lên :
17/03/2014, 03:20
... interacts with JC virus large T-antigen Virology 219, 237–246 52 Vacante, D.A., Traub, R & Major, E.O (1989) Extension of JC virus host range to monkey cells by insertion of a simian virus 40 enhancer ... governed by a host factor such as nuclear factor I (NF-I) required to facilitate initiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting ... 270) 2035 This view is supported by the recent finding that the JC virus receptor is widely distributed on cells which contrasts with the cellular restriction of virus propagation [54] Factors involved...
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Variation of Chest Radiographic Patterns in Pulmonary Tuberculosis by Degree of Human Immunodeficiency Virus–Related Immunosuppression pot
Ngày tải lên :
29/03/2014, 03:20
... with x2 and Fisher’s exact tests Confidence intervals for unadjusted odds ratios were calculated by Woolf’s method [13] Logistic regression was done to examine the independent relationship of CD4/ ... positive smears (15% vs 4.6%; P Å 07) Table shows the frequency of specific radiographic findings by CD4/ cell count strata Among patients with abnormal chest radiographic findings, 67 (57%) had ... smear – positive, two also had extrapulmonary TB, none had endobronchial TB, and all 10 were deemed by their providers to clinically have TB on the basis of a constellation of signs and symptoms Those...
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Báo cáo khóa học: Conformational changes of Newcastle disease virus envelope glycoproteins triggered by gangliosides pdf
Ngày tải lên :
30/03/2014, 13:20
... gangliosides As deduced by PAGE analysis (data not shown), treatment of viruses with 2-mercaptoethanol led to the loss of F0 protein In another series of experiments, viruses were incubated in ... continuously, as described in Materials and methods, by measuring the dequenching of R18 (d) Control; (m) virus and gangliosides with preincubation; (j) virus and gangliosides without preincubation Fig ... although it has been established that for viruses that fuse with the target membrane through a pH-independent mechanism, such as most paramixoviruses and retroviruses, the conformational change of...
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase pot
Ngày tải lên :
30/03/2014, 20:20
... the template but not by premature termination or internal initiation, as suggested in reports for tomato bushy stunt virus, cucumber necrosis virus [38] and hepatitis C virus [39] Taken together, ... been described for some viruses [51,52] Interaction of the viral proteins NS3 and NS5 has been reported in Japanese encephalitis virus [53], Dengue virus [54] and hepatitis C virus [55,56] Nevertheless, ... hepatitis C virus and pestivirus NS5B RNA polymerase activity by GTP J Biol Chem 274, 10807–10815 Xiao, M., Zhang, C.Y., Pan, Z.S., Wu, H.X & Guo, J.Q (2002) Classical swine fever virus NS5B-GFP...
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