virus based nanoparticles as tools for biomedicine

... characterization of neuronally active a-conotoxins Assay -based and cDNA -based strategies The first a-conotoxins were identified using bioassays such as intraperitoneal (neuromuscular nAChRs) or intracranial ... (micromolar) affinity As the bioassays on state, whereas [A10L]PnIA stabilizes a desensitized state fish and insects as well as intracranial injections into rats which, in the case of the a7[L247] ... caused an increase in potency as arise primarily from a3b2 and a3b4 nAChRs Activity on well as a shift in selectivity: while PnIA inhibited an a-BTXa7 nAChRs was excluded for two reasons: (a) EpI...

Ngày tải lên: 19/02/2014, 12:20

... 0.25 °CÆmin)1 was therefore preferred for subsequent experiments with GT TFOs Padlock formation with TC oligonucleotides The use of TC TFOs has not been previously described for the formation of ... 5350 Cleveland, OH, USA) (1 U for XmnI, 12 U for DraIII, 2.5 U for XbaI) and incubating for h at 37 °C Phosphatase was inactivated for 20 at 65 °C DNA was then ethanol precipitated and resuspended ... 3B), as observed for the GT oligonucleotides However, we noticed that a long heating in the acidic buffer resulted in plasmid nicking Therefore, a cooling rate of °CÆmin)1 was prefered for subsequent...

Ngày tải lên: 20/02/2014, 03:20

... micelles, the ethaselen-loaded micelle solution was lyophilized using PEG6000 as a lyoprotectant, and then dissolved in DMSO by ultrasonication for 15 min, and ethaselen content was measured with ... were then calculated based on the following formula: LCð%Þ mass of ethaselen extracted from freezeÀdried micelles ¼ total mass of freezeÀdried micelle Â100% EE ð%Þ mass of ethaselen extracted from ... compatibility between the micelle core-forming PLA and the ethaselen molecules In previous work, our group has prepared micellar ethaselen formulation employing PLA -based copolymers and characterized...

Ngày tải lên: 22/06/2014, 00:20

... significantly increased frequency of CD19+/CD27high plasma cells and had a predictive value for disease activity that was greater than traditional humoral/clinical measurements (for example, anti-dsDNA, ... association with lupus disease activity indices or other clinical features was determined High IFN scores were positively associated with increased disease activity as indicated by the SLEDAI ... and none has as yet been qualified as a biomarker that can predict clinical outcomes Autoantibody profiles A number of previous studies reported that SLE patients can be dissected based on their...

Ngày tải lên: 09/08/2014, 14:22

... were first perfused with PBS for 15 and then with 4% formalin in PBS for 30 Brains were carefully removed, post-fixed for hours in 4% formalin in PBS, equilibrated for 24 h in 30% sucrose in PBS ... glioblastoma multiforme: biological and clinical results Cancer gene therapy 2005, 12(10):835-848 doi:10.1186/1743-422X-7-298 Cite this article as: Redaelli et al.: Bovine herpesvirus based vector ... bregma, 3.7 mm below the pial surface Injection was carried out for 16 minutes and was performed using a Hamilton syringe Animals were monitored daily for neurological signs and weight loss At the...

Ngày tải lên: 12/08/2014, 02:20

... Nanoparticles as Adsorbents for Metal Ion Removal 12 2.2.1 Iron -based Nanoparticles 13 2.2.1.1 Introduction 13 2.2.1.2 Pristine zero-valent iron (Fe0) nanoparticles 16 2.2.1.3 Modified iron -based ... iron -based nanoparticles have been extensively studied in their synthesis methods and structure-property relationships The reliable performance and wide acceptance has rendered iron -based nanoparticles ... iron oxide nanoparticles For example, a Ni(II) sequestration capacity of 130 mg/g was obtained for the core/shell iron nanoparticles, whereas a modest value of 25 mg/g was reported for maghemite...

Ngày tải lên: 10/09/2015, 15:54

... between GF and XML was seen from the beginning, and GF was designed as a formalism for multilingual rendering of semantic content (Dymetman and al 2000) XML originated as a format for structuring ... languages, separate for each pair of languages Being interlingua -based, GF translation scales up linearly to new languages without the quadratic blowup of transfer -based systems In transfer -based sys4 ... framework is sufficient for both kinds of tasks One important source of inspiration for GF was the WYSIWYM system (Power and Scott 1998), which used domain-specific interlinguas and produced excellent...

Ngày tải lên: 17/03/2014, 00:20

... facts in the market, and based on a commitment to transparency, they will create more powerful, lasting impacts For Further Information The staff at the Purdue Center for Regional Development ... of the workforce resides outside of the city’s borders Understanding and developing the workforce requires a regional perspective A Knowledge -Based, Innovation-Driven Economy The four tools are ... improvisations are not free-form and chaotic; they are based on a foundation The structure gives players a focus within which to develop their ideas In the end, sound strategies adapt as circumstances change...

Ngày tải lên: 17/03/2014, 10:20

... maintained for at least weeks with no appearance of new disease CR + PR was rated as the overall response rate Stable disease (SD) was defined as a less than 50% reduction or less than 25% increase ... complete response (CR) was defined as the disappearance of all lesions and no appearance of new disease for at least weeks Partial response (PR) was defined as a reduction by at least 50% in the sum ... dexamethasone mg bid 24 hours before each infusion of docetaxel up to a total of six doses Treatment was given for a maximum of cycles and was discontinued in cases of unacceptable toxicity, disease...

Ngày tải lên: 18/06/2014, 15:20

... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... mg/ml MTT solution was added to each well for incubation h Finally, the MTT was removed, and 200 μl DMSO was added to dissolve the MTT-formazan crystals The absorbance was measured at 490 nm by...

Ngày tải lên: 18/06/2014, 19:20

... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... mg/ml MTT solution was added to each well for incubation h Finally, the MTT was removed, and 200 μl DMSO was added to dissolve the MTT-formazan crystals The absorbance was measured at 490 nm by...

Ngày tải lên: 20/06/2014, 03:20

... water each for 30 After drying in the air, the FTO glass was immersed in Au NP colloid solution at 70°C for 30 TiO2 film was prepared by screen printing of TiO2 paste on the FTO glass, followed ... citrate tribasic dehydrate, potassium carbonate anhydrous and tannic acid [26] The concentration of the Au NPs is about 0.3 mM Prior to the fabrication of TiO2 film, FTO glass was ultrasonically ... the performance enhancement of the cell Experimental FTO glass (resistivity: 14 X/h, Nippon Sheet Glass, Japan) was used as the substrate for nanocrystalline TiO2 (P25, Degussa) electrodes Cadmium...

Ngày tải lên: 21/06/2014, 07:20

... that the iron nanoparticles act as nucleation and aggregation sites for carbon and the impending formation of diamond-type bonds The formation of a dense, mixed C phase around the Fe nanoparticles ... region) A fast Fourier transform analysis was employed to measure the spacing between the atomic planes, which were found to vary The lattice spacing in different regions was measured to be 1.1, 1.16, ... nucleation centers for subsequent growth [31] Thus the formation of a dense amorphous carbon phase is a plausible route for nucleating diamond The iron nanoparticle may act as catalyst in decomposing...

Ngày tải lên: 21/06/2014, 22:20

... method was followed in order to study the antibacterial activity of Ag nanoparticles [8] Luria–Bertani (LB) medium has been prepared as follows: g of tryptone, g of NaCl, 0.5 g of yeast extract ... agent, is necessary for the formation of Ag nanoparticle However, in air atmosphere, silver nanoparticles have not been observed When the refluxing has been Fig TEM pictures of Ag nanoparticles prepared ... [15] as well as thermal decomposition has taken place to yield Ag Nanoscale Res Lett (2007) 2:44–48 agglomeration of particles, in this case, is possible Hence, the bigger particles have been formed...

Ngày tải lên: 22/06/2014, 22:20

... was observed only for seeds of F angustifolia (for all progeny arrays) and St-Dyé and Tavaux seed lots Moreover, the mean germination rate was two-fold higher for F angustifolia seeds than for ... BLAST algorithm at http://www.ncbi.nlm.nih.gov (National Center for Biotechnology Information), with programs blastn, tblastn and tblastx New primers were redefined on the three sequences FaH299, ... population: 25.4) from three areas of sympatry between these two species (Tab II) A bi-marker phenotype was assigned to each individual (0 for the absence and for the presence of the dominant...

Ngày tải lên: 07/08/2014, 16:20

... potential of nanoparticles As such, the cytotoxicity of nanoparticles should be thoroughly tested before their application in sunscreens Due to the increased toxicity associated with internalized nanoparticles, ... detected by UV detector, and as a result, were not collected for the Proteinase K digestion The long-term objective of this study is to propose proteinbased ivy nanoparticles for UV protection Degradation ... risk for the metal -based and engineered nanoparticles in sunscreens has led to the search for alternative replacement nanoparticles In this study, naturally occurring ivy nanoparticles were investigated...

Ngày tải lên: 11/08/2014, 00:22

... existing measurement tools has confirmed differences in emphasis across the domains Measurement tools for RA and RU focus more on access to new information, leadership, and resources for change, ... from the measurement tools For two tools [105,106], the full text of each item was not available, so the names of the categories of measurement for which results were reported were used as items, ... institutional audit and comparison Tools are available to measure the organizational context for evidence -based healthcare practice [38-41], and components of evidencebased practice (EBP) including...

Ngày tải lên: 11/08/2014, 05:21

... existing measurement tools has confirmed differences in emphasis across the domains Measurement tools for RA and RU focus more on access to new information, leadership, and resources for change, ... from the measurement tools For two tools [105,106], the full text of each item was not available, so the names of the categories of measurement for which results were reported were used as items, ... institutional audit and comparison Tools are available to measure the organizational context for evidence -based healthcare practice [38-41], and components of evidencebased practice (EBP) including...

Ngày tải lên: 11/08/2014, 16:20

... expressed as mean ± SD, and in figures as mean ± SE As Dm is influenced by fibrosis, correction for fibrosis was performed by multiple regression, where Dm was the dependent variable, and disease type ... before PAH was diagnosed[2,13] However, TLCO has not been established yet as a marker for SScPAH It remains to be elucidated how interstitial lung disease influences TLCO in these patients as ... decrease in capillary flow and thus a decrease in Vc This will result in a reduction in surface area available for gas exchange, and therefore in a decrease of Dm [25] Secondly, parenchymal and vascular...

Ngày tải lên: 12/08/2014, 14:20

... cut-off values for outcome associations The optimal cut-off was defined as the value associated with the highest sum of sensitivity and specificity (Youden’s index) Analysis was performed using ... venous PCO as a surrogate for mixed venous PCO2 [21], has recently been proposed as a useful tool for GDT in ICU-septic patients to identify persistent hypoperfusion when a ScvO2 > 70% has been ... of coronary artery disease Perioperative management was similar in all patients except for the basal rate of intraoperative crystalloids Data collection and outcome measures Preoperatively, patients...

Ngày tải lên: 13/08/2014, 21:21