viral surface glycoproteins in carbohydrate recognition structure and modelling

... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with...

Ngày tải lên: 14/02/2014, 19:20

... [20]; and finally the C-terminal domain is rich in arginin and glycin residues and is able to interact with ribosomal proteins [21] or ligands like lactoferrin [13] Interestingly, the central domain ... cell surface signaling receptors following ligand binding [14] This implies a redistribution of nucleolin at the surface of cells following ligand binding, and a possible role of glycosylation in ... central domain is involved in the protein self-interactions Interestingly, preliminary studies show that N-glycosylation may modulate binding of the nucleolin-derived protein to heparin (data not...

Ngày tải lên: 14/03/2014, 23:20

... the cooked kernels and a tunable (see below) cooking behavior; and can experience industrial processes like cooking, freezing and canning without significant loss of kernel integrity [5,6,7] Parboiled ... 10 sliding blades (HDP/KS10, StableMicroSystems, UK) A 100 g lot of rice was previously cooked in boiling water for the desired cooking time and then drained for a given time (6 min) and finally ... Results and Discussion Starch Gelatinization White rice: Starch gelatinization in white rice is sustained by the water intake and progresses from the kernel surface toward the core The water intake...

Ngày tải lên: 19/03/2015, 13:36

... tests by using some modification measures, such as improving the cell design and testing methodology, employing novel experimental techniques and computerized testing machine, and taking the wall ... usually very complex and not straightforward in physical meaning Since manufacturers and plant engineers often prefer a more direct or simple methodology in designing and sizing filtration equipment, ... Chemical and Environmental Engineering, National University of Singapore Without their kind and helpful support, I would not be able to carry out my work smoothly and in good order And, my special...

Ngày tải lên: 17/09/2015, 17:18

... of Water and Environment Technology, Vol.2, No.2, 2004 Therefore, we investigated successional development of nitrifying bacterial community structure and in situ nitrifying activities in biofilms ... activity Combination of FISH and microsensor technology became a powerful and reliable tool during the last two decades The structure and principle of commonly used amperometric and potentiometric ... (PCR) using a diverse set of primers and separated by several fingerprinting techniques including denaturing gradient gel electrophoresis (DGGE) (Muyzer and Smalla, 1998), temperature gradient...

Ngày tải lên: 05/09/2013, 08:40

... employed in the kitchen for food washing, cooking, dish washing, and boiling water, 27% for showering and foot washing, 18% for toilet flushing, and 14% for the washing machine Water use in washing and ... water, face washing, tooth-brushing, using washing machine, showering, foot washing, toilet flushing, and cleaning According to Murakawa et al (1999), water use patterns differ according to the purpose ... Water use for cooking in Beijing (31%) is 1.3 times that of Tokyo (23%) It is assumed that water use for cooking in Beijing includes food washing, cooking, dish washing, and boiling water The use...

Ngày tải lên: 05/09/2013, 10:17

... especially financial accounting reports businesses often grasp of existing capital, both in value and in kind, forming the source and the increased volatility, reduced capital during the period, ... settlement and clearing system, shortening transaction time, establishing independent depository institutions It also should develop market intermediaries and institutional investors including stock ... supply, in effect expanding money or contracting the money supply This involves meeting the demand of base money at the target interest rate by buying and selling government securities, or other financial...

Ngày tải lên: 26/12/2013, 15:19

... in elderly patients taking risperidone in clinical studies † The US Food and Drug Administration issued a health advisory in March 2005 reporting increased mortality (1.6 -1.7 fold increase in ... Drug Interactions Major drug interactions associated with memantine include drugs which increase the pH in urine (e.g carbonic anhydrase inhibitors) Exercise caution when prescribing memantine ... contributing to cognitive decline, and; b Identify neurologic abnormalities including localizing signs, extrapyramidal signs and ataxia Cognitive Impairment in the Elderly – Recognition, Diagnosis and...

Ngày tải lên: 13/02/2014, 18:20

... with incentives for firms to reduce accounting information quality Blindly -4- adopting international accounting standards and disclosure rules without regard to the institutional environment in ... (Jacobson and Aaker, 1993; Ali and Hwang, 2000) In addition, instead of using NI in our regression model, we have also used ∆NI, change in earnings (current earnings minus lagged earnings all ... accounting earnings: international evidence Journal of Accounting and Economics 29, 1-52 Ball, R., A Robin, and J Wu, 1999 Properties of accounting earnings in four East Asian countries Working...

Ngày tải lên: 18/02/2014, 01:20

... X-ray structure with °C NMR structure using the main chain of residues Ser12fiThr70 in the structure alignment (B) Overlap of the X-ray structure with the 30 °C NMR structure using the main chain ... Ser12fiThr70 in the structure alignment (C) Overlap of X-ray structure of TmAFP with the NMR structure determined at 30 °C using the main chain of residues Gln1fiGly80 in the structure alignment Fig Insect ... interacts with the ice surface It is important to distinguish whether the mutations disrupt the ice–binding interaction by changing the surface properties of the protein, or by altering the structure...

Ngày tải lên: 19/02/2014, 16:20

... reported structure of diol dehydratase [9] In addition, aG and bG are longer by one amino acid than aD in the N-terminal and by three than bD in the C-terminal, respectively In the final structure, ... residues interacting with the substrate (green) and K+ (cyan) are shown in ball-andstick models Cobalamin, pink (B) Residues hydrogen-bonded to cobalamin The residues interacting with cobalamin from ... containing 10 mM imidazole and 300 mM KCl After washing the column with buffer A containing 10 mM imidazole and 300 mM KCl, the enzyme was eluted with buffer A containing 50–100 mM imidazole and...

Ngày tải lên: 21/02/2014, 03:20

... and jcasein ROA bands are therefore assigned to PPII structure The positive ROA bands in b- and j-casein at » 1290± 1295 cm)1 may originate in other types of loops and turns A negative ROA band ... residues, as observed in the caseins [3], synucleins [10], tau [14], prothymosin a [7] and the ®bronectin-binding protein [8], and also in typical chemically denatured proteins [56±58] Over extensive ... a-synuclein and the A30P and A53T mutants, as well as in b- and c-synuclein, is PPII helix ROA measurements on tau protein Six isoforms of tau protein, ranging from 352 to 441 amino acids in length,...

Ngày tải lên: 21/02/2014, 15:20

... domains The N-terminal domain is implicated in stop codon recognition [5–14] The role of the middle (M) domain will be described in detail below The C domain of eRF1 interacts with the C domain ... may have resulted in a decrease in the overall quality of the structure Finally, the C and N domains may have structural in uences on the M domain within the whole eRF1 protein The pairwise comparison ... the protein during the docking to the binding site on the ribosome or other ligands Strikingly, the second most flexible part of the protein (if one does not take into account the N-terminal region...

Ngày tải lên: 07/03/2014, 05:20

... nucleolin) was retained on an affinity matrix containing purified Lf In view of this and of a previous observation pointing out that nucleolin serves as a binding protein for various ligands, we investigated ... crosslinking of a ligand leads to the clustering or capping of its surface receptor Accordingly, we investigated the distribution of surface nucleolin following the crosslinking of bound hLf using ... using HB-19-fluo in Ó FEBS 2003 Nucleolin is a cell surface lactoferrin-binding site (Eur J Biochem 271) 311 Fig Location of nucleolin-binding sites in both lobes, but not in the basic N-terminus...

Ngày tải lên: 07/03/2014, 15:20

... understanding in such a theory Most obviously, where in the past parsing and phonological processing have tended to deliver conflicting structural analyses, and have had to be pursued independently, ... simply that particular surface structures that are induced by the specific combinatory grammar that are postulated to explain coordination in English subsume the intonational structures that are ... (31) Anything which can coordinate can be an intonational constituent, and vice versa What the isomorphism between syntactic structure and intonational structure does mean is that simply structured...

Ngày tải lên: 08/03/2014, 18:20

... Therefore, the carbohydrate metabolic disorder and insulin resistance were proved in the STZ induced diabetic mice Table Blood glucose and insulin concentration after days of buffer or STZ injection ... diet according to National Institute of Nutrition (NIN) and Srinivasan et al [5] Animals had free access to diet and water (ad libitum) in weeks extracts, diabetic mice were divided into different ... increased slightly (about 1.5 times, p > 0.05) as compared to buffer injected ND fed mice untreated with STZ and equivalent to HFD In addition, serum insulin level in HFD mice significantly increase...

Ngày tải lên: 14/03/2014, 10:20

... households and firms are mainly confronted with retail bank interest rates when making investment and savings decisions Corporate financing is predominantly bank rather than market-based and euro ... testing and error correction modelling, Applied Economics, 31, 267-277 Wong, K.P., 1997, On the determinants of bank interest margins under credit and interest rate risks, Journal of Banking and ... continuing decline in money market interest rates during the years of nominal convergence, a reversal took place in the course of 1999 As our baseline estimation was shown to be unstable in a...

Ngày tải lên: 15/03/2014, 14:20

... domains of the proteases Intermodular and intramodular flexibilities are thought to be essential in these movements Expression of recombinant intact C1r [4] and its domain combinations in insect ... comprising mainly the b-strands The loops between strands B and C, known as the ‘hypervariable loop‘, D and E, E and F, F and G, and G and H are particularly tolerant of longer insertions [19] In ... at and near histidines (H335 and H348), these residues are strong candidates for inducing minor conformational changes and ⁄ or forming pH-dependent interaction sites on CCP1 Surface turns and...

Ngày tải lên: 15/03/2014, 23:20

... ˚ R357 (2.7 A) and points away from E53 (5 A) In the external aldimine structure, Y61 points towards E53, approaching its side-chain carboxylate group ˚ and Cb of the bound ligand, l-Ser (2.8 ... residue involved in proton abstraction from the hydroxyl group of l-Ser and other 3-hydroxy amino acids A detailed examination of the binary and ternary complexes of bsSHMT has Role of Y51 and Y61 in ... claim to original Indian government works 4607 Role of Y51 and Y61 in bsSHMT catalysis B S Bhavani et al A I external Aldimine - 425 L-Ser II Gly quinonoid - 495 nm III Carbinolamine IV Iminium cation...

Ngày tải lên: 16/03/2014, 06:20

... structure and function in several integral membrane proteins [15–20] This approach has proved to be very successful in providing qualitative structural information and in guiding complete structure ... contrast, the N-terminal residues (Val2 and Leu4–Leu9) almost retained their intensities, indicating that the N-terminus was embedded in the SDS micelles and was solvent inaccessible When Mn2+ ... (v/v) containing mM peptide and 300 mM SDS-d25 at pH 6.0, and 150 ms in TFE-d2 The NOE connectivities and numbers of NOEs per constraints for DMT1-TM4 in both solvents are summarized in Fig Except...

Ngày tải lên: 16/03/2014, 16:20