vip in models of autoimmunity inflammation and bone marrow transplantation

... between baseline and months decrease in the population of memory B cells both in the PB and in the BM of RA patients Depletion of total and activated B cells is a specific effect of rituximab ... B cells both in the PB and in the BM of RA patients (d) Representative flow cytometry histograms of HLA-DR expression in PB and BM CD19+-gated cells of a RA patient at baseline and after treatment ... pathogenesis is the aberrant antigen-presenting capacity of B cells, contributing to chronic T-cell activation and perpetuation of inflammation in the joint We Page of (page number not for citation purposes)...

Ngày tải lên: 09/08/2014, 14:22

... common to stem cell and bone marrow transplantation In our experience, no single clinical handbook currently provides the bone marrow transplantation clinician with comprehensive information relevant ... management following bone marrow transplant (BMT) 411 411 Page i A Clinical Guide to Stem Cell and Bone Marrow Transplantation Page Introduction to Stem Cell and Bone Marrow Transplantation The ... 97-9563 Printed in the United States of America 01 00 99 98 10 Page v Disclaimer The nature of clinical bone marrow transplantation information is that it is constantly evolving because of ongoing...

Ngày tải lên: 10/08/2014, 08:20

... nystatin, oral amphotericin, clotrimazole, and cyclosporine c) GVHD of the upper gastrointestinal tract d) Hepatic disease including GVHD e) Infections involving the esophagus, stomach, or intestine ... MB Autologous bone marrow transplantation: clinical indications, treatment process and outcomes In: Whedon MB, ed Bone Marrow Transplantation: Principles, Practice and Nursing Insights Boston: ... course and variation in pain and nausea Bone Marrow Transplant 1989;4:181–186 Epstein JB Infections prevention in bone marrow transplantation and radiation patients NCI Monogr 1990;9:73–85 Kolbinson...

Ngày tải lên: 10/08/2014, 08:20

... marrow spaces following intravenous infusion Engraftment of stem cells begins within one to two weeks after infusion in most patients Page 123 References Patterson K Bone marrow harvesting In: ... stabilized in the recovery room and then usually admitted to begin conditioning therapy III Complications of Bone Marrow Harvesting A The potential complications of bone marrow harvesting are as ... effect of prophylactic intravenous immune globulin on the incidence of septicemia in marrow transplant recipients, Bone Marrow Transplant 1987;2:141–148 Gale RP, Winston D Intravenous immunoglobulin...

Ngày tải lên: 10/08/2014, 08:20

... presumed and systemic antifungal therapy initiated in the form of amphotericin B (minimum of 0.5 mg/kg/d) Page 142 Table 5.4 Clinical Features and Common Signs and Symptoms of Infection in BMT ... conditioning regimen and other marrow- suppressive medications b) Conditioning results in an absence of WBCs including granulocytes, monocytes/macrophages, and lymphocyte (Infections resulting from ... streptomycin possible, may use capreomycin or kanamycin instead of streptomycin or Isoniazid, rifampin, pyrazinamide, & streptomycin qd for mo, then isoniazid & rifampin times/wk for 10 mo * Capreomycin,...

Ngày tải lên: 10/08/2014, 08:20

... GVHD but include diarrhea and abdominal pain Malabsorption and submucosal fibrosis are seen in advanced cases l) Liver: Increased bilirubin and elevation in alkaline phosphatase, often out of proportion ... seemingly "foreign" host, resulting in varying degrees of severity b) Damage occurs to three target organs: the skin, gastrointestinal tract, and liver c) Acute GVHD is defined as occurring within ... with a history of P aeruginosa chest infection and low serum IgG Other rare pulmonary findings Page 193 include lymphoid interstitial pneumonitis and pulmonary fibrosis n) Vagina: Inflammation, ...

Ngày tải lên: 10/08/2014, 08:20

... of bone marrow transplantation Bone Marrow Transplant 1991;8: 159–170 Bosi A, Vannucchi AM, Grosi A Serum erythropoietin levels in patients undergoing autologous bone marrow transplantation Bone ... In: Spivak JL, Barnes HV, eds Manual of Clinical Problems in Internal Medicine 4th ed Boston: Little, Brown and Co; 1990:514 Chisholm, DJ Endocrine complications In: Atkinson K, ed Clinical Bone ... Common to Bone Marrow and Stem Cell Transplants 1, 2, 3, 4, Disclaimer: Drug information is constantly evolving because of ongoing research and clinical experience and is often subject to interpretation...

Ngày tải lên: 10/08/2014, 08:20

... q4–6 h (PO or IV) Continuous infusion: Begin at 0.25 mg/h and titrate for comfort (no more than incremental increases of 0.25–1 mg/h in a 24 h period) Lidocaine and prilocaine Topical cream: Children ... agranulocytosis, proteinuria, titrate dose in twofold of taste increments to max dose of mg/kg/d in 1–4 divided doses or 75 mg/d Class: antihypertensive angiatensin converting enzyme (ACE) inhibitor Use ... Cream) prilocaine lidocaine 2.5% & prilocaine 2.5% (5, 30 g with Tegaderms) SE: Contact dermatitis, burning, stinging, angio urticaria Apply thick layer of cream to intact skin and cover with...

Ngày tải lên: 10/08/2014, 08:20

... achieving effective intervention Assessment of pain should address onset, duration, location, radiation, quality, intensity, pattern, aggravating factors, and alleviating factors intensity of pain ... use of a numeric scale in adolescents and adults In children, the use of a visual scale may be helpful B In BMT pain is generally the result of the underlying disease The toxicities inherent in ... Nausea and vomiting In: Herfinadal ET, Gourley DR, eds Textbook of Therapeutics: Drugs and Disease Management 6th ed Baltimore: Williams and Wilkins; 1996 McIntyre WJ, Parr MD Infections in the...

Ngày tải lên: 10/08/2014, 08:20

... responding to call buttons in a timely fashion, waking patients up in the early hours of the morning, inability to get a central line to draw blood) Acknowledging real mistakes and apologizing minimize ... consequences of bone marrow transplantation in children: a preliminary communication J Psychosoc Oncol 1987;5:73–81 Andrykowski A Psychiatric and psychosocial aspects of bone marrow transplantation ... and patient pain complaints must be balanced against the risks of potentiating abuse, consultation with mental health professionals specializing in substance abuse is indicated Page 426 IX Interventions...

Ngày tải lên: 10/08/2014, 08:20

... the N7 position of guanine Alkylation of guanine results in depurination by excision of guanine residues, causing DNA strand breakage through scission of the sugar-phosphate backbone [3] Patients ... Use of Live Animals in Teaching and Research of the University of Hong Kong Male ICR mice (weighing 25–30 g) were reared on a standard laboratory diet (Ralston Purina, Chicago, Illinois, USA) and ... shown to protect mice against hydrocoticoid-induced inhibition of IFN-γ, IL-2, IL-4 and IL-10 transcription [24] In addition, vitamin B12, folinic acid and biotin identified in AP may also contribute...

Ngày tải lên: 02/11/2012, 10:19

... that selected sigma-2 ligands are capable of inducing apoptosis in a multitude of human and murine cancer cells lines and in animal models of cancer including pancreas cancer [12,13,15] The mechanisms ... obtained from Bryan Clary (Duke University) and maintained in supplemented RPMI 1640 containing glutamine (2 mmol/L), pyruvate (1 mmol/L), penicillin (100 IU/ mL), streptomycin (100 IU/mL), and ... pancreatic adenocarcinoma cell lines (Panc-1, AsPC-1, and CFPAC-1) were obtained from ATCC (Bethesda, MD) and maintained in Dulbecco's modified eagle's medium (DMEM) containing glutamine (2 mmol/L),...

Ngày tải lên: 18/06/2014, 15:20

... demonstrated in the analysis of the role of IFN-γ in models of autoimmunity Early attempts to define the role of IFN-γ in autoimmunity by the administration of IFN-γ or neutralizing antibodies ... data and the studies from many others using murine models of autoimmunity that cytokine circuits involved in the regulation of humoral and cell-mediated immune responses in the development of autoimmune ... functional roles of cytokines in the pathogenesis of autoimmunity The original description of Th1 and Th2 responses has provided us with a valuable framework for advancing our understanding of pathogenic...

Ngày tải lên: 09/08/2014, 06:22

... identified citrulline-modifications in multiple proteins in inflamed CIA joint and EAE brain tissue Our results suggest that citrullination of synovial proteins in CIA and brain proteins in EAE generate ... applied synovial and myelin protein arrays to investigate the development and evolution of ACPA responses in rodent models of RA and MS, with the objective of gaining further insights into the aetiology, ... citrullinated filaggrin, a protein expressed in stratified epithelium but not in joints [4,6] It is interesting that many of the citrullinated proteins identified in inflamed CIA joint tissue and...

Ngày tải lên: 09/08/2014, 13:22

... were ligated and excised The left hindlimb was kept intact and used as the nonischemic limb Simvastatin administration and MSCs transplantation Simvastatin administration and MSCs transplantation ... higher in simvastatin + Figure Effect of simvastatin and bone marrow- derived MSCs administration on angiogenesis in ischemic limb A Representative microphotographs of the section of ischemic hindlimb ... option to induce therapeutic angiogenesis The main advantage of using bone marrow- derived MSCs in treating ischemic disease is that they can be isolated from bone marrow by aspiration and expanded...

Ngày tải lên: 10/08/2014, 05:21

... the 18 th day and Stereotaxic single dose of μl of 5-HT (10 μg/μl), GABA(10 μg/μl) and 10 μl of Bone marrow cell (BMC) (106 Cells/ml) suspension individually and in combination (combinational treatment) ... were intranigrally infused with 6-OHDA (8 μg in μl) Bone marrow cell and neurotransmitters were infused individually and in combination in denervated striatum on the 18th day Animals were injected ... of 0.25 to 50 nM at 23°C in an assay medium containing 10 mM HEPES, pH 7.0, 200 - 250 μg of protein, 100 μM glycine and 100 μM glutamate Specific [ H] MK-801 binding was obtained by subtracting...

Ngày tải lên: 10/08/2014, 05:21

... assessed the intensity of fatigue before and after a minute walk test (6MWT) using the Borg scale [20] Patients rated their feeling by the selection of one option, ranging from 0-12, with meaning no ... in the median of the inflammatory biomarkers in quartiles of physical and cognitive Borg fatigue scores at baseline had a weak positive correlation with TNF-a and CRP (rho = 0.24, p = 0.01, and ... found in multivariate analyses Discussion We explored the association between systemic inflammation and depression and fatigue using a range of inflammatory biomarkers, two depression scales and...

Ngày tải lên: 12/08/2014, 13:22

... differences in the expression of cytokines involving chemokines in the BALF As shown in Fig 4A, the expression of several cytokines and chemokines in the BALF from WT and CD69-/- mice was induced ... Role of interleukin-6 in bleomycin-induced lung interleukinbleomycinmice 38(5):566-571 inflammatory changes in mice Am J Respir Cell Mol Biol 2008, 38 34 Cavarra E, Carraro F, Fineschi S, Naldini ... role in the development of allergen-induced eosinophilic inflammation [29] These findings led us to consider that the gene disruption of CD69 could affect the pathogenesis of pulmonary inflammatory...

Ngày tải lên: 12/08/2014, 14:20

... following injection in hepatic portal vein [12] We and others have shown that removal of PIMs with gadolinium chloride or clodronate inhibits endotoxin-induced lung inflammation[ 13,14] Interestingly, ... were processed and embedded in paraffin Sections (five micron) were cut onto the polyL-Lysine coated slides and used for hematoxylin and eosin staining and immunostaining Pieces of the left lung ... or presence of PIMs PIM reduction protects against LPS-induced mortality in BDL rats We delineated role of PIMs by comparing LPS-induced lung inflammation and mortality in BDL rats and GCtreated...

Ngày tải lên: 12/08/2014, 14:20

... Burlingame, CA, USA) for 15 minutes Thereafter, the sections were rinsed in PBS (3 × minutes) before blocking of biotin-binding sites with biotin blocking solution (Vector Laboratories) for 15 minutes ... of 0.28 ml 51 mM Na2CO3, 0.20 M Na2SO4 and 0.22 M NaCl, and cells were washed in PBS and fixed in PBS containing 1% formaldehyde prior to analysis Intracellular staining was performed using IntraPrep™ ... epithelial cell line [30] In the present study, expression of TLR9 was found in the endothelial lining of small blood vessels This finding is in line with the detection of TLR9 mRNA in mouse lung...

Ngày tải lên: 12/08/2014, 15:20