venue s early to cushion your budget

... required as an input, easiness to understand and use, and so forth The most essential factors for choosing the MCA technique is the easiness of understanding by the analyst, the stakeholders and use ... sediments at the bed of the river, and reduce the opportunities for the ships to pass satisfactorily This necessitates building a decision support tool so as to be used by the construction engineers ... is the human expert s opinions on which measures should be used to minimise stormwater pollution due to erosion and sedimentation generated from Malaysian construction sites Small groups of stakeholders...

Ngày tải lên: 05/09/2013, 16:11

... This command resets the switch with factory defaults All system parameters will revert to their default factory settings All static and dynamic addresses will be removed Reset system with factory ... parameters set to factory defaults All other parameters will be unchanged Reset system with VTP parameters set to factory defaults, [Y]es or [N]o? Enter y and press Enter Remove the switch startup ... Series switches 2900 and 2950 Series Switches Enter into the privileged EXEC mode by typing enable If prompted for a password, enter class (if that does not work, ask the instructor) Switch>enable...

Ngày tải lên: 05/11/2013, 12:15

... overall system performance must be considered Costs and business results are a consequence of our business system design, that is, our processes Why Do Executives Persist in Cost Cutting? The data suggest ... was not sustainable As one of them suggested, our costs are the consequence of our business system design, our processes and practices, and we need to improve those It also seemed that the businesses ... these senior managers and built into the systems already established in the company, which, in turn, suggests that changing the business system design is a better strategy Initial impressions...

Ngày tải lên: 04/12/2013, 23:54

... hosts and switches are correctly configured, ping the switches from the hosts b Were the pings successful? c If the answer is no, troubleshoot the hosts and switches ... Step Configure the hosts attached to the switches Configure the host to use the same subnet for the address, mask, and default gateway as on the switch Step Verify connectivity a To verify ... by using default values, is not the best choice It is necessary to force the “other” switch to become the root switch b In the example output given the root switch by default, is Switch_A Switch_B...

Ngày tải lên: 11/12/2013, 14:15

... } Discussion While it is possible to locate, sort, and filter records in a DataTable or DataView, there is no method in either class to select the top n rows The procedure to get the user-specified ... Convert.ToInt32(topNTextBox.Text); if(topN

Ngày tải lên: 14/12/2013, 18:16

... cathepsin B as deeply as stefin B does to cathepsin H To illustrate this, we calculated the average distances between CA atoms of the active site cysteine and histidine residues in cathepsins B and ... only as much as is mandatory Results and Discussion Crystals of the complex of stefin A and cathepsin B contain complete wild-type protein sequences The positioning of the main chains of nearly ... endogenous protein inhibitors cystatins and stefins [21] A further step in understanding of these mechanisms was provided by the crystal structures of human [22] and rat procathepsins B [23] They...

Ngày tải lên: 18/02/2014, 04:20

... restraints from NOEs Total distance restraints from disulfide bonds Total distance restraints from hydrogen bonds Total distance restraints No restraints ⁄ residue Table 1B Calculation statistics ... 3D structure that are characteristic of the ribotoxin family are present in HtA Analysis of these assignments provides some interesting clues concerning HtA structure First, several protons show ... microenvironment in a-sarcin [23,24] that is responsible for its efficient cytotoxic action Discussion As is very well documented, ribotoxins and nonspecific ribonucleases show high structural homology...

Ngày tải lên: 18/02/2014, 08:20

... interest because this residue has functional homologs in all FNR-like structures Moreover, the cysteine and glycine at this position are part of one of the consensus sequences that define the structural ... volume M A Musumeci et al Leu268 is replaced by a serine in the bacterial reductases of subclass I (Azotobacter vinelandii) and by an asparagine in the bacterial reductases of subclass II (Escherichia ... group This system has been used previously to analyze the geometry of the tyrosine stacked on the si-face of the flavin in FNRs [12] The single point energies of the flavin–tyrosine system in different...

Ngày tải lên: 18/02/2014, 17:20

... as tyrosinase Mushroom tyrosinase is included in Table because it is the most commonly used tyrosinase in model studies It is important to note that the most characteristic signatures in the sequences ... monophenolase vs o-diphenolase differences are in bold and higher size Consensus shows the concordance with the conserved positions for tyrosinases [6] A novel tyrosinase from Rastonia solanacearum ... according to Table 1, this residue is conserved only in plant catechol oxidases Tyrosinases from Streptomyces have G at that position, animal tyrosinases generally have Q, and mushroom tyrosinase has...

Ngày tải lên: 19/02/2014, 07:20

... the residues proposed to be involved – Asp75, Asn78 and Asn122 N-glycosidase activity of RTA variants with substitutions at Asp75 and Asn78 Substitution of either Asp75 or Asn78 with Ala resulted ... expression levels and purification of these variants was not achieved However, although a very low expression level was again seen on substitution of Asp75 to Ser, substitution of Asn78 to Ser ... expressed at very low levels, and purification to homogeneity, to allow quantitative activity assays of these RTA variants, was not achieved To assess whether substitution at Asn78 with Ser changed...

Ngày tải lên: 19/02/2014, 12:20

... Bacterial strains and plasmids used in this study Strain or plasmid Strains R capsulatus B10 BSE16 JP91 Plasmids pUC18 pFRK-I pRK2013 pSE50 pSE102 pPHU231 pOD585 pSE504 pSE103 pOD7 pOD12 pSE60 pOD15 ... possible to design a hydrogenase that exhibits high activity together with O2 insensitivity Experimental procedures Bacterial strains and plasmids The strains and plasmids used in this study are listed ... gases, they probably use the same access pathway to the hydrogenase active site The amino-acid sequences of the O2-resistant RHs have been compared with those of the O2-sensitive hydrogenases...

Ngày tải lên: 20/02/2014, 01:20

... residues in sequences in the alignment by CLUSTALW; (:), conserved substitutions; (.), semiconserved substitutions Residues conserved in all aligned sequences are printed in bold, the conserved sequence ... appears improbable This too is consistent with the E1 crystal structures in that the arginine residues corresponding to E1aR282 of the B stearothermophilus E1 are not pointing towards the active site, ... 2-oxo acid substrate thus appears to be very likely The crystal structures of E1 show the corresponding arginine residues in P putida [14] and human [15] E 1s to be in a suitable position to participate...

Ngày tải lên: 20/02/2014, 23:20

... dialysis, which contained 0.1% SDS, was not degraded at all (data not shown) SDS/PAGE analysis of the degradation of SulA by Lon protease Using SulA3±169 as substrate, its in vitro sensitivity toward ... D113 Q90 S6 1 G160 SulA, hence presumably possessing the native or native-like structure SulA23±169 also showed essentially the same CD spectrum and sensitivity toward Lon protease as SulA3± 169, ... consensus residues or sequences of residues were found around the cleavage sites However, it should be noted that as for the six major sites, Leu was predominant at the P1 position and Ser was...

Ngày tải lên: 21/02/2014, 03:20

... these approaches can be found in (Purandare and Pedersen, 2004) Thus, in total there are 22 words to be discriminated, with senses, words with senses, with senses, and words with senses Four different ... data sets, making it hard to apply a single threshold The graph of the P K1 scores shown in Figure illustrates the difficulty - the slope of these scores is nearly linear, and as such the threshold ... values, and had fewer spurious responses (very large or very small predictions) We note that the distribution of P K2 s predictions were most like those of the actual senses Conclusions This paper...

Ngày tải lên: 22/02/2014, 02:20

... involved in cytochrome c binding We also assayed the oxidase mutants under presteadystate conditions, to ensure that the observed effects indeed relate to the early phases of electron entry Using the ... ancillary subunits may contribute to some extent to the interaction domain for the substrate (see also Discussion) Kinetic parameters for both complexes under several ionic strength conditions are listed ... was 2.5 mM Tris/HCl, pH 7.5, 0.2 gÆL)1 dodecyl maltoside Stopped-flow kinetics The presteady-state kinetics were followed on a thermostated Applied Photophysics DX.117 MV stoppedflow apparatus...

Ngày tải lên: 22/02/2014, 07:20

... Women Yes Yes No Yes Yes Yes No Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No No Yes Yes Yes No Yes No Yes NO No No No No No It may therefore be concluded that direct measurements serve to perfectly ... contribute to the second J Tesedo Nieto et al TST AST BST Second factor Second factor 0.0 SuST SST -0.4 WOMEN AGE -1 MEN W -2 H -0.8 MAC -1.0 -2 -0.5 0.0 -1 0.5 First factor First factor Fig 3.—Scores ... however, there is no general consensus as to the variables that should be measured or calculated, or as to the age groups of subjects that should be considered.6 If both these issues were standardized,...

Ngày tải lên: 05/03/2014, 21:20

... unfolding As previously demonstrated by ITF, ligand-free WT hGK senses temperature shifts from to 39 °C directly by a slow (seconds to minutes) conformational change (hysteresis), with a biphasic time ... mutant, respectively c Assay with Glc as the variable substrate d Assay with ATP as the variable substrate e Obtained from equilibrium binding measurements by intrinsic Trp fluorescence spectroscopy ... interacts with the substrate and cosubstrate in a random fashion [13], enzyme kinetic studies support an ordered mechanism in which Glc binds to the enzyme before the cosubstrate [14– 16] The discussion...

Ngày tải lên: 06/03/2014, 00:20

... as the buffer The eluted peaks were collected as fractions of mL for subsequent analysis by 4–12% SDS/PAGE Synthetic peptides Peptides were synthesized as amides by solid-phase peptide synthesis ... MBL is dependent on its associated serine proteases, the mannan-binding lectin-associated serine proteases (MASPs), of which three forms (MASP-1, MASP-2 and MASP-3) have been described, together ... by two genes Immunobiology 205, 455–466 Sorensen R, Thiel S & Jensenius JC (2005) Mannanbinding-lectin-associated serine proteases, characteristics and disease associations Springer Semin Immunopathol...

Ngày tải lên: 07/03/2014, 05:20

... was seen only with selected substrates, so both kynuramine and MPTP were tested for MAO-A As some MAO-A inhibitors (such as the b-carbolines [28], also known to bind to both I2BS and MAO-A [8]) ... Lake, MN, USA) for helpful discussion and suggestions on the physicochemical properties of the guanidine derivatives The modeling work is part of Laura Giurato s PhD thesis, to be ` presented at ... and its stereoisomers, new alpha-2-antagonists J Cardiovascular Pharmacol 7, S1 27 S1 29 Tan AK, Weyler W, Salach JI & Singer TP (1991) Differences in substrate specificities of monoamine oxidase-A...

Ngày tải lên: 07/03/2014, 10:20