validation of biochemical markers by bone histomorphometry

... the variability of FibroTest and ActiTest in relation to the method of expressing enzymatic activity; in particular, in terms of absolute values or as multiples of the upper limit of normal Since ... definitions of normal values may be inappropriate [8– 10], a major concern was the definition of ALT and GGT activity In routine practice, the definition of the upper limit of normal (ULN) of ALT ... observed a mean occurrence of pain in 30 % of patients, out of 1,000 endured severe adverse events, and out of 10,000 died [2] There is no ideal gold standard for the assessment of liver histology Even...

Ngày tải lên: 13/08/2014, 13:20

... calories of the meal was 782 kilo-calories with a maximum of 1340 kcal, it is prudent to recommend a meal of around 800 kcal Conclusions This study shows that the biochemical markers of liver ... long-term medication use of the contraceptive pill and the ingestion of a NSAID (ibuprofen) for pelvic pain 48 hours before sampling The aim of this study was to assess the impact of meal intake on ... strongly suggest that due to the limitations [4-6] and risks of biopsy [7], as well as the improvement of the diagnostic accuracy of biochemical markers [8,9], liver biopsy should no longer be considered...

Ngày tải lên: 13/08/2014, 13:20

... details were given concerning the causes of discordance between biopsy and biochemical markers However FT-AT is the only panel of markers identified by an independent overview [9], which has ... cutoff (91%), as was the negative predictive value for excluding significant activity at the 0.36 cutoff of AT (85% negative predictive value) The positive predictive value of the 0.72 cutoff of ... cut off of 0.36, the ActiTest negative predictive value for excluding significant necrosis (prevalence 0.41) was 85% (Table 2) Comparison of FT-AT diagnostic values with other biochemical markers...

Ngày tải lên: 13/08/2014, 13:20

... Fragments of CAPs markers digested with Afl III and Nde I A CAPS profile generated by digestion with restriction enzyme Afl III Representative samples of each Boehmeria lines are shown on top of the ... 100 bp Figure Quality control of admixed DNA samples A Quality test of CAPS markers Digestion pattern with restriction enzyme Afl III of the CAPS profile generated by admixed DNA samples from both ... size of DNA standards M: DNA markers B CAPS profile generated by digestion with restriction enzyme Nde I Li et al Chinese Medicine 2010, 5:40 http://www.cmjournal.org/content/5/1/40 A Page of...

Ngày tải lên: 13/08/2014, 14:20

... analyses Bone biomarkers Biochemical markers of bone turnover were assayed in archived serum and urine stored at -70°C We measured two serum bone formation markers: osteocalcin and bone- specific ... areas of bone necrosis are also present, as is scar The extensive bone remodeling seen, and the correlation of MRI bone marrow lesions with positive bone scintigraphy (a tracer that is picked up by ... MRI We hypothesized that if bone marrow lesions are areas of accelerated bone turnover, then they should be accompanied by elevation in systemic levels of bone turnover markers If our hypothesis...

Ngày tải lên: 09/08/2014, 13:21

... the coordination of the speakers and the recognised with the appearance of adjacency pairs, so it is preceded by a series of turns The control of this process is turn taking Schegloff [33] points ... the coordination of the speakers and the recognised with the appearance of adjacency pairs, so it is preceded by a series of turns The control of this process is turn taking Schegloff [33] points ... terms of the aspects mentioned in the aims and objectives In addition, because of its emphasis on in-depth knowledge and elaboration of concept as well as description of frequency of occurrence of...

Ngày tải lên: 26/11/2013, 12:42

... endotoxins), the validation of the sterility testing in compliance with eu Pharmacopoeia 2.6.1 (sterility) and the validation of the potency assay in an ATMP that is constituted of bone- marrow mononucleated ... (bioMerieux SA, Switzerland) Validation test Validation was performed by direct inoculation of TSB and FTM media with 1% of the total volume of the product under validation as stated in European Pharmacopoeia ... characterized by the presence of the CD34 cell-surface antigen (found in about 1% of human bone marrow mononucleated cells); it has therefore been considered a useful cell selection target for bone marrow...

Ngày tải lên: 18/06/2014, 15:20

... were of a functional feature of DC maturation The amount of IL-12 in culture supernatants of BMDCs was assessed by ELISA Compared with the control, LPS Page of promoted significantly secretion of ... disintegrated by vigorous pipetting The bone marrow cell suspension was centrifuged at 300 × g for The cells were collected, suspended in PBS by addition of red blood cell lysate for depletion of erythrocytes, ... scanning size of × μm and the mature BMDCs (L) in the scanning size of × μm; F and M, Enlarged view of the center of the immature BMDCs (F) and the mature BMDCs (M) in the same scanning area of × μm;...

Ngày tải lên: 21/06/2014, 02:20

... schematic of the solid–vapour–solid mechanism of growth of 1D nano-structure from a spherical layer of atoms in a tip growth 123 This corresponds to the number of atoms in the ith segment of the rod of ... CuKa wavelength of 0.154184 nm The experimental procedure showed good reproducibility of results Lengths and corresponding diameters of the nano-wires were measured by means of a software package ... assembled into the rod of length l1, the next layer in the sphere has a radius of R0-X which forms the next segment of the rod of length l2 The subsequent layers have radii of R0 - 2X, R0 - 3X,...

Ngày tải lên: 22/06/2014, 01:20

... tissue inhibitor of metalloproteinases biochemical markers of bone metabolism are also proposed as indicators of disease progress of OA [14,15] Spector and colleagues demonstrated that bone resorption ... mostly women and characterized by the development of Heberden nodes and a specific pattern of OA showing multiple joint involvement Because levels of serum biochemical markers for arthritis depend ... 11 12 13 14 15 16 17 18 Vol No Takahashi et al tion of baseline levels of markers of bone and cartilage degradation with long-term progression of joint damage in patients with early rheumatoid...

Ngày tải lên: 09/08/2014, 01:23

... comparison of the early events of infection between E urophylla and both strains of P tinctorius shows important differences at the interface Indeed, it seems that the plant reacts to the presence of ... controlled utilization of mycorrhizal symbionts in plantation man- agement not have any knowthe nature of the signal from each organism, which initiates this succession of early events of mycorrhizal ... Lapeyrie F & Garbaye J (1989) Infectivity of two isolates of Pisolithus tincto- rius isolated from beneath pine and eucalypts respectively on roots of Eucalyptus urophylla in vitro Mycorrhizal...

Ngày tải lên: 09/08/2014, 04:20

... and bones of TNF-transgenic mice stromal cellsstromal cell-derived factor-1 expression in bone marDecreased stromal cell-derived factor-1 expression in bone marrow row stromal cells and bones of ... strongly expressed by osteoblasts on endosteal and trabecular bone surfaces of murine long bones (Figure 5, arrows) TNF treatment was associated with loss of SDF-1-positive staining Page of 10 (page ... expression by TNF injection stromal cellsstromal cell-derived factor expression marrow decreases by bone marrow stromal cells Expression of stromal cell-derived factor (SDF-1) protein by bone marrow...

Ngày tải lên: 09/08/2014, 10:23

... space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic ... determination of the threshold cycle at which exponential amplification of PCR products begins Specificities of amplicons were confirmed by melting curve analyses by gel electrophoresis of test PCR ... mL by optical density at 260 nm and standard plaque assay Culture of human bone marrow-derived MSCs and adenoviral transduction Bone marrow was harvested from the surgical waste of femurs of...

Ngày tải lên: 09/08/2014, 14:22

... portion of the genome plays a major role in the evolution of species [54] This is the first report of development and validation of a comprehensive set of genic-SSR markers in pigeonpea by deep ... of 20-25 bases with an optimum of 22 bases; PCR product size range of 100-200 bp; optimum annealing temperature of 50-60°C; GC content of 40-60% with an optimum of 50%; the specified number of ... were compared by BLASTX tool of NCBI at a cutoff bit score of 50 against the non-redundant protein database SSR marker scoring and data analysis The genotype profiles produced by SSR markers were...

Ngày tải lên: 11/08/2014, 11:21

... stopped with 50 μL of 2N H2SO4, and optical density (OD) was read at 490 nm Confirmation of negative-positive cutoff The negative-positive cutoff value was set by the average OD ratio of 25 field negative ... average OD of positive sera tested on GST alone was not significant different from that of negative sera tested on GST alone (P > 0.05) Confirmation of negative-positive cutoff A cutoff point ... coefficient of variation (CV) of three replicates of each test were calculated Evaluation of assay specificity and sensitivity The diagnostic sensitivity (DSn) and specificity (DSp) of the ELISA...

Ngày tải lên: 12/08/2014, 01:22

... correlation of those values in humans with a wide range of illnesses and injured lungs This correlation was also unaffected by sex, degree of LIS, pleural fluid amount, degree of CI, degree of CVP, ... correlation coefficients was tested by the z test after Gaussian transformation of the coefficients Reproducibility of EVLW measurements was assessed by the coefficient of variation (CV) and intraclass ... arterial partial pressure of oxygen/fraction of inspired oxygen Figure Correlation of extravascular lung water (EVLW) measured by single transpulmonary thermodilution and by postmortem lung weight...

Ngày tải lên: 13/08/2014, 21:21

... increased bone remodeling in DMP1-caPTHR1 is partially corrected by the removal of DCR 18 Figure 10: The high bone formation exhibit by DMP1-caPTHR1; DCR-/- was reduced by removing ... skeleton contributes up to 15 to 20 percent of the total human weight The skeleton is made of different types of bone: cancellous (trabecular) bone and cortical bone The skeleton plays an important ... concentration of these important ions in body fluids” (1) Moreover, the bones contribute in the body movement by increasing the force that is generated by contraction of the skeletal muscle Figure 1: Bone...

Ngày tải lên: 24/08/2014, 09:46

... chromatograms (a) chromatogram of blank plasma, (b) chromatogram of blank + IS, (c) chromtogram of LLOQ Development and validation of analytical method for Naftopidil in human plasma by LC–MS/MS lots were ... study of naftopidil in human plasma showed reliable stability behavior, as the mean of the results of the tested samples was within the acceptance criteria of ±15% of the initial values of the ... specificity of the method was investigated by comparing chromatograms obtained from six different sources of plasma The area observed at the retention time of naftopidil was much less than 20% of the...

Ngày tải lên: 02/09/2015, 13:48

... improved the PPV of our effectiveness algorithm by 6%, but limits generalizability as it excluded one-third of our data The strengths of our study include evaluation of a large number of patients ... characteristics of the eligible cohort remained similar (right-most column of Table 2) The primary results of the study are shown in Table Among biologic users (Table 3), a total of 28% of treatment ... independent of RA disease activity As part of a sensitivity analysis, we restricted the cohort to patients without any of these ICD-9 codes as part of a sensitivity analysis As part of two additional...

Ngày tải lên: 25/10/2012, 10:45

... toxicity of CY on bone marrow by measuring WBC number in the peripheral blood Twenty microliters of blood was mixed with 380μl of Randolph’s solution WBC counting was then performed by using an improved ... in depurination by excision of guanine residues, causing DNA strand breakage through scission of the sugar-phosphate backbone [3] Patients under chemotherapeutic regimen are often subject to ... administration This study was conducted with the consent of the Committee on the Use of Live Animals in Teaching and Research of the University of Hong Kong Male ICR mice (weighing 25–30 g) were reared...

Ngày tải lên: 02/11/2012, 10:19