v d j recombination in precursor b cell acute lymphoblastic leukemia

... therapy was initiated Day four chemotherapy had to be omitted because of the patient’s bilirubin level of 11 mg/dL By day 11, his bilirubin had dropped to mg/dL, and vincristine was administered with ... they have no competing interests Received: 28 November 2010 Accepted: July 2011 Published: July 2011 References Scheimberg IB, Pollock DJ, Collins PW, Doran HM, Newland AC, van der Walt JD: Pathology ... accompanying images A copy of the written consent is available for review by the Editor -in-< /b> Chief of this journal Abbreviations Hyper-CVAD: hyperfractionated cyclophosphamide/vincristine/doxorubicin/ dexamethasone;...

Ngày tải lên: 10/08/2014, 23:21

... and Cell Cultures) or were generously provided by the original investigators Detailed references and cultivation protocols have been described previously [9] SET-NUP214 screening and breakpoint ... leukemia/ lymphoma cell lines of T-, B- and myeloid cell origin to detect SET-NUP214 positive examples A T-ALL cell line LOUCY (1/43 T cell lines tested) and an AML cell line MEGAL (1/53 myeloid cell lines ... expression was performed as follows: × 106 cells were pelleted and washed with ice-cold phosphate-buffered saline (PBS), resuspended and boiled for 10 in < /b> 25 μl SDS sample buffer containing 15% glycerol,...

Ngày tải lên: 10/08/2014, 22:20

... Previous studies [21,22] showed that the inhibition of BCL1 1B expression by siRNA selectively inhibited proliferation and effectively induced apoptosis in < /b> human T -cell acute lymphoblastic leukemia ... precisely determine the copy numbers of BCL1 1B, a duplex vector, including a fragment of the BCL1 1B and the b2 microglobulin (b2 M) genes was constructed and used as a reference (the duplex vector ... lymphoid development Nat Immunol 2003, 4:525-532 Przybylski GK, Dik WA, Wanzeck J,< /b> Grabarczyk P, Majunke S, MartinSubero JI, Siebert R, D< /b> lken G, Ludwig WD, Verhaaf B, van Dongen JJ, Schmidt CA,...

Ngày tải lên: 10/08/2014, 22:21

... Stovall M, Vik TA, Inskip PD, Robison LL, Childhood Cancer Survivor Study: Obesity in < /b> adult survivors of childhood acute lymphoblastic leukemia: A report from the childhood cancer survivor study ... contributions SS designed and coordinated the study, collected the follow-up information, performed data analysis and drafted the manuscript, PT designed biochemical methods and performed biochemical ... analysis, performed data analysis and participated in < /b> drafting of the manuscript MB-M designed genotyping methods and performed genotyping, performed data analysis and participated in < /b> drafting of the...

Ngày tải lên: 10/08/2014, 10:21

... study because BAFF level could have been modulated by disease activity and perhaps by treatment BAFF serum level remained correlated with T allele even in < /b> patients without antiSSA/SSB autoantibodies, ... and analyzed and interpreted the data JS, MI, FL, AP, HZ, CS, JS and RP performed acquisition of data Acknowledgements We are indebted to Dr Jean-Pierre Hugot (INSERM U458, Hôpital Robert Debré, ... Wheway J,< /b> et al.: Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome J < /b> Clin Invest 2002, 109:59-68 Jonsson MV, Szodoray P, Jellestad S, Jonsson...

Ngày tải lên: 09/08/2014, 07:20

... adhesion molecule capable of mediating both cell- to -cell interactions, via binding to vascular -cell adhesion molecule-1 (VCAM-1), and interactions with extracellular matrix components by binding ... segments involved in < /b> V(< /b> D)< /b> J < /b> recombination < /b> confer diversity by random and imprecise rearrangement during B- cell development in < /b> the bone marrow The consequent protein sequences mainly differ in < /b> the complementary-determining-region-3 ... several genes often involved in < /b> cell cycle and/or programmed cell death regulation such as MCL1, ETS1 and JUN [16,18-20] Additional studies are needed to identify the genes actually involved in...

Ngày tải lên: 18/06/2014, 15:20

... KG1a cells Curcumin decreased Bcl-2 mRNA and protein levels and reduced MMP in < /b> both DNR-insensitive and -sensitive AML cell lines The mechanisms underlying curcumin-induced apoptosis were investigated ... (Figure 3C) Both Annexin -V/< /b> PI and Western blotting showed that curcumin induced apoptosis in < /b> a dose-dependent manner U937cells were the most sensitive to curcumin-induced apoptosis, followed by Kasumi-1, ... U937 Annexin Daunorubicin( g/ml) + Figure CD34 KG1a and Kasumi-1cells were insensitive to DNR (A) KG1a, Kasumi-1 and U937 cells were stained with PE-conjugated CD34 antibody and subjected to flow...

Ngày tải lên: 18/06/2014, 19:20

... 32-191005-Vysis Following hybridization, the excess and unbound probe was removed by a series of washes and the chromosomes and nuclei were counter stained with DNA specific stain DAPI (4.6 diamidino-2-phenylindole) ... platelet-derived growth factor receptor β in < /b> CMML, to the ABL tyrosine kinase in < /b> acute myeloid leukemia and ALL, and to the product of the MNI gene in < /b> myeloproliferative disorders AML-I is the DNA-binding ... JE, Downing JR, Pui CH, Shurtleff SA, Raimondi SC, Evans WE, Head DR, Crist WM, Rivera GK, Hancock ML, Boyett JM, Buijs A, Grosveld G, Behm FG: TEL gene rearrangement in < /b> acute lymphoblastic leukemia: ...

Ngày tải lên: 10/08/2014, 22:20

... treatment in < /b> developing countries N Engl J < /b> Med 2005, 352:2158-60 Rajajee S, Desikulu MV, Pushpa V:< /b> Survival of childhood acute lymphoblastic leukemia: experience in < /b> Chennai J < /b> Trop Pediatr 1999, ... second step, the Bahasa Indonesia version was translated back to English version and it was done independently by two local professional translators, native English speakers, and bilingual in < /b> Bahasa ... approved by the Medical Ethics Committees of Faculty medicine, Gadjah Mada University Informed consents were also obtained from the guardians tion (3 items) Both instruments were available for...

Ngày tải lên: 18/06/2014, 19:20

... W, van den Berg H, van den Berg E, B kkerink J,< /b> Bruin M, van den Heuvel-Eibrink M, Korbijn C, Korthof E, van der Pal K, Stijnen T, van Weel Sipman MH, van Weerden JF, van Wering ER, van der Does-van ... longitudinal associations between persistent sleep problems in < /b> childhood and anxiety and depression disorders in < /b> adulthood J < /b> Abnorm Child Psychol 2005, 33(2):157-163 12 Liu X, Hubbard J,< /b> Fabes R, Adam ... 33(3):298-306 Sadeh A, Sivan Y: Clinical practice: sleep problems during infancy Eur J < /b> Pediatr 2009, 168(10):1159-1164 Ali NJ, Pitson DJ, Stradling JR: Snoring, sleep disturbance, and behaviour in < /b> 4-5...

Ngày tải lên: 20/06/2014, 15:20

... treatment in < /b> developing countries N Engl J < /b> Med 2005, 352:2158-60 Rajajee S, Desikulu MV, Pushpa V:< /b> Survival of childhood acute lymphoblastic leukemia: experience in < /b> Chennai J < /b> Trop Pediatr 1999, ... second step, the Bahasa Indonesia version was translated back to English version and it was done independently by two local professional translators, native English speakers, and bilingual in < /b> Bahasa ... approved by the Medical Ethics Committees of Faculty medicine, Gadjah Mada University Informed consents were also obtained from the guardians tion (3 items) Both instruments were available for...

Ngày tải lên: 20/06/2014, 16:20

... lymphoblastic leukemia (ALL) patients undergoging total body irradiation (TBI) precondionting as human beings in < /b> vivo irradiation model The advantage of using this model lies in < /b> full view of in < /b> vivo ... probe conjugated with minor groove binder (MGB) groups, which is more sensitive and appropriate than nonspecific dsDNA-binding dye PCR methods previously used [16] Besides, we taken the acute lymphoblastic ... Mitochondrial DNA (mtDNA) alterations, including mtDNA copy number and mtDNA 4977bp common deletion (CD), are key indicators of irradiation-induced damage The relationship between total body irradiation...

Ngày tải lên: 09/08/2014, 09:21

... and less well-defined backup end-joining pathways, are increased in < /b> FLT3-ITD cells [50] Cells treated with an FLT3 inhibitor exhibit decreased DNA ligase IIIa expression and a reduction in < /b> DNA ... Levis M, Allebach J,< /b> Tse KF, Zheng R, Baldwin BR, Smith BD, Jones-Bolin S, Ruggeri B, Dionne C, Small D:< /b> A FLT3-targeted tyrosine kinase inhibitor is cytotoxic to leukemia cells in < /b> vitro and in < /b> ... genomic instability driven by higher endogenous ROS, increased DNA damage and decreased end-joining fidelity Further analyses from the same research group using FLT3-ITD-expressing cell lines and bone...

Ngày tải lên: 10/08/2014, 21:23

... Armitage JO, Carbone PP, Connors JM, Levine A, Bennett JM, Kroll S: Treatment-related myelodysplasia and acute leukemia in < /b> non-Hodgkin’s lymphoma patients J < /b> Clin Oncol 2003, 21:897-906 Macdonald DR: ... Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, Belanger K, Brandes AA, Marosi C, Bogdahn U, Curschmann J,< /b> Janzer RC, Ludwin SK, Gorlia T, Allgeier A, Lacombe D,< /b> Cairncross JG, Eisenhauer ... repair eventually lead to breaks and permanent nicks in < /b> the daughter strand, preventing mitotic division, and the cell undergoes apoptosis [7,8] The action of TMZ has been shown to be augmented in...

Ngày tải lên: 11/08/2014, 03:21

... Armitage JO, Carbone PP, Connors JM, Levine A, Bennett JM, Kroll S: Treatment-related myelodysplasia and acute leukemia in < /b> non-Hodgkin’s lymphoma patients J < /b> Clin Oncol 2003, 21:897-906 Macdonald DR: ... Mason WP, van den Bent MJ, Weller M, Fisher B, Taphoorn MJ, Belanger K, Brandes AA, Marosi C, Bogdahn U, Curschmann J,< /b> Janzer RC, Ludwin SK, Gorlia T, Allgeier A, Lacombe D,< /b> Cairncross JG, Eisenhauer ... repair eventually lead to breaks and permanent nicks in < /b> the daughter strand, preventing mitotic division, and the cell undergoes apoptosis [7,8] The action of TMZ has been shown to be augmented in...

Ngày tải lên: 11/08/2014, 07:20

... resistance Individualized medicine and childhood acute lymphoblastic leukemia for implementing individualized medicine the following issues need to be addressed: The patients exhibit variable treatment ... face in < /b> individualized medicine, and how they can be addressed Childhood acute lymphoblastic leukemia In < /b> the industrialized countries cancer is the most common medical cause of death in < /b> children ... with the administered drug dose Whereas individualized ALL therapy based on genetic markers this far has been limited to avoiding acute toxicities by adjusting 6MP doses by TPMT genotyping [12],...

Ngày tải lên: 13/08/2014, 13:20

... CCAAT/enhancer binding protein (CEBP) family such as CEBPα/β/ε in < /b> myeloid development have been suggested in < /b> mice models where various abnormalities in < /b> myeloid lineage development have been observed in < /b> CEBPα/β/ε ... LIST OF ABBREVATIONS A Alanine AML acute myeloid leukaemia APL acute promyelocytic leukaemia BSA bovine serum albumin DAPI 4,6-diamidino-2-phenylindole DMSO dimethyl sulfoxide DNA deoxyribonucleic ... classified into subtypes as determined based on morphology, cellularity, blast percentage and cytochemistry These subtypes are distinguished based on both the degree of differentiation and cell lineage...

Ngày tải lên: 09/09/2015, 18:55

... identified optimal L) boundaries to separate the samples of D0< /b> and D8< /b> , D8< /b> and D1< /b> 5, and D1< /b> 5 and D3< /b> 3, respectively 34 Figure 4.2: Leukemic genetic signatures are dissolved into the background during ... D0< /b>  D8< /b>  D1< /b> 5  D3< /b> 3 by unsupervised hierarchical clustering To quantitatively describe the significance of the observation and to find out the optimal boundaries between the adjacent time points, ... Relapses are marked in < /b> red S1, S2 and S3 are the identified optimal L) boundaries to separate the samples of D0< /b> and D8< /b> , D8< /b> and D1< /b> 5, and D1< /b> 5 and D3< /b> 3, respectively complete linkage as the linkage (Eisen...

Ngày tải lên: 10/09/2015, 15:50

... the inhibitors survivin, IAP2 and ICAD (and probably FLIP) Thus, E2A is involved in < /b> fine control of pre-mature B- cell apoptosis mediated by BCR signaling via transcriptional regulation of survivin, ... SDS–PAGE followed by immunoblotting Antibody binding was detected using secondary antibodies conjugated to horseradish peroxidase, and then data analysis was performed using a luminescent image ... Rag-1, Vj1 and jo, that are involved in < /b> D-< /b> JH rearrangement, cell survival, Igj rearrangement, etc [26,27] E2A directly controls IgH gene expression, and is involved in < /b> repressing the Nfil3 and FGFR2...

Ngày tải lên: 16/03/2014, 04:20